12,748 results on '"rice blast disease"'
Search Results
2. Deciphering early responsive signature genes in rice blast disease: an integrated temporal transcriptomic study
- Author
-
Antony, Ajitha, Veerappapillai, Shanthi, and Karuppasamy, Ramanathan
- Published
- 2024
- Full Text
- View/download PDF
3. Preparation, antifungal potentiality, and mechanisms of action of agronanofungicides in controlling rice blast disease and enhancing yield of rice
- Author
-
Kutawa, Abdulaziz Bashir, Ahmad, Khairulmazmi, Hussein, Mohd Zobir, Ali, Asgar, Wahab, Mohd Aswad Abdul, Rafi, Amara, Gunasena, Mahesh Tiran, Zobir, Syazwan Afif Mohd, and Berahim, Zulkarami
- Published
- 2024
- Full Text
- View/download PDF
4. Effectors and environment modulating rice blast disease: from understanding to effective control.
- Author
-
Kou, Yanjun, Shi, Huanbin, Qiu, Jiehua, Tao, Zeng, and Wang, Wenming
- Subjects
- *
RICE blast disease , *PYRICULARIA oryzae , *PLANT diseases , *GENOME editing , *GENE silencing - Abstract
Disease outbreaks are shaped by the 'disease triangle,' comprising a virulent pathogen, a susceptible plant, and appropriate environmental conditions. Recent advances have shed light on the intricate environment–plant–pathogen interactions. Rice resistance is manipulated by cytoplasmic and apoplastic effectors secreted by Magnaporthe oryzae. Environmental factors play a significant role in both blast disease epidemics and the regulation of host (or rice) resistance. Strategies such as protein engineering, genome editing, and host-induced gene silencing have been employed effectively to engineer blast-resistant rice plants. Rice blast is a highly destructive crop disease that requires the interplay of three essential factors: the virulent blast fungus, the susceptible rice plant, and favorable environmental conditions. Although previous studies have focused mainly on the pathogen and rice, recent research has shed light on the molecular mechanisms by which the blast fungus and environmental conditions regulate host resistance and contribute to blast disease outbreaks. This review summarizes significant achievements in understanding the sophisticated modulation of blast resistance by Magnaporthe oryzae effectors and the dual regulatory mechanisms by which environmental conditions influence rice resistance and virulence of the blast fungus. Furthermore, it emphasizes potential strategies for developing blast-resistant rice varieties to effectively control blast disease. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
5. Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis.
- Author
-
Devanna, Basavantraya Navadagi, Sucharita, Sumali, Sunitha, N. C., Anilkumar, C., Singh, Pankaj K., Pramesh, D., Samantaray, Sanghamitra, Behera, Lambodar, Katara, Jawahar Lal, Parameswaran, C., Rout, Prachitara, Sabarinathan, Selvaraj, Rajashekara, Hosahatti, and Sharma, Tilak Raj
- Subjects
- *
RICE blast disease , *GENE regulatory networks , *PLANT diseases - Abstract
Rice blast disease is the most devastating disease constraining crop productivity. Vertical resistance to blast disease is widely studied despite its instability. Clusters of genes or QTLs conferring blast resistance that offer durable horizontal resistance are important in resistance breeding. In this study, we aimed to refine the reported QTLs and identify stable meta-QTLs (MQTLs) associated with rice blast resistance. A total of 435 QTLs were used to project 71 MQTLs across all the rice chromosomes. As many as 199 putative rice blast resistance genes were identified within 53 MQTL regions. The genes included 48 characterized resistance gene analogs and related proteins, such as NBS–LRR type, LRR receptor-like kinase, NB-ARC domain, pathogenesis-related TF/ERF domain, elicitor-induced defense and proteins involved in defense signaling. MQTL regions with clusters of RGA were also identified. Fifteen highly significant MQTLs included 29 candidate genes and genes characterized for blast resistance, such as Piz, Nbs-Pi9, pi55-1, pi55-2, Pi3/Pi5-1, Pi3/Pi5-2, Pikh, Pi54, Pik/Pikm/Pikp, Pb1 and Pb2. Furthermore, the candidate genes (42) were associated with differential expression (in silico) in compatible and incompatible reactions upon disease infection. Moreover, nearly half of the genes within the MQTL regions were orthologous to those in O. sativa indica, Z. mays and A. thaliana, which confirmed their significance. The peak markers within three significant MQTLs differentiated blast-resistant and susceptible lines and serve as potential surrogates for the selection of blast-resistant lines. These MQTLs are potential candidates for durable and broad-spectrum rice blast resistance and could be utilized in blast resistance breeding. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Key transcription factors required for outburst of rice blast disease in Magnaporthe oryzae
- Author
-
Wang, Qing, Huang, Zhicheng, Khan, Irshad Ali, Li, Yan, Wang, Jing, Wang, Jiaoyu, Liu, Xiao-Hong, Lin, Fucheng, and Lu, Jianping
- Published
- 2024
- Full Text
- View/download PDF
7. Impact of Epigenetic Regulatory Genes on Biological Functions in Magnaporthe oryzae the Causal Agent of Rice Blast Disease.
- Author
-
Hendy, Ahmed, Shama, Saad, Amer, Mostafa, and Xiao-Lin Chen
- Subjects
- *
RICE blast disease , *PYRICULARIA oryzae , *REGULATOR genes , *PATHOGENIC fungi , *HOST plants - Abstract
Magnaporthe oryzae is a heterotrophic plant pathogenic fungus that developed the capability to colonies of living plant cells, A lot of genes play key roles in biological function, especially during the invasion process and biotrophic growth. but the mechanisms through which they establish bio-trophic growth by modifying gene expression are still unclear, we conducted an integrated analysis involving PRC2 genes which includes this description focusing on SUZ 12, EZH2 and EED1 proteins, revealing its crucial effect in the development and infection processes of the rice blast fungus, Magnaporthe oryzae. Deletion of SUZ 12, EZH2 and EED1 in M. oryzae resulted in a modest reduction in vegetative growth and a significant decrease in conidiation. Notably, the SUZ 12, EZH2 and EED1 mutants exhibited a marked reduction in virulence towards host plants. Observation of the infection process indicated that the mutants were halted in invasive growth, leading to the accumulation of substantial host reactive oxygen species (ROS). Additionally, the SUZ 12, EZH2 and EED1 mutants displayed sensitivity to cell wall-disturbing agents, These results suggest that the PRC2 complex plays an important role in biotrophic growth, thereby facilitating invasive growth in M. oryzae. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Antifungal plant flavonoids identified in silico with potential to control rice blast disease caused by Magnaporthe oryzae.
- Author
-
Moin, Abu Tayab, Robin, Tanjin Barketullah, Patil, Rajesh B., Rani, Nurul Amin, Prome, Anindita Ash, Sakif, Tahsin Islam, Hossain, Mohabbat, Chowdhury, Dil Umme Salma, Rashid, Shah Samiur, Mollah, A. K. M. Moniruzzaman, Islam, Saiful, Uddin, Mohammad Helal, Khalequzzaman, Mohammad, Islam, Tofazzal, and Islam, Nazneen Naher
- Subjects
- *
RICE blast disease , *PYRICULARIA oryzae , *ROSMARINIC acid , *FUNGICIDE resistance , *FUNGICIDES , *GIBBS' free energy - Abstract
Rice blast disease, caused by the fungus Magnaporthe oryzae, poses a severe threat to rice production, particularly in Asia where rice is a staple food. Concerns over fungicide resistance and environmental impact have sparked interest in exploring natural fungicides as potential alternatives. This study aimed to identify highly potent natural fungicides against M. oryzae to combat rice blast disease, using advanced molecular dynamics techniques. Four key proteins (CATALASE PEROXIDASES 2, HYBRID PKS-NRPS SYNTHETASE TAS1, MANGANESE LIPOXYGENASE, and PRE-MRNA-SPLICING FACTOR CEF1) involved in M. oryzae's infection process were identified. A list of 30 plant metabolites with documented antifungal properties was compiled for evaluation as potential fungicides. Molecular docking studies revealed that 2-Coumaroylquinic acid, Myricetin, Rosmarinic Acid, and Quercetin exhibited superior binding affinities compared to reference fungicides (Azoxystrobin and Tricyclazole). High throughput molecular dynamics simulations were performed, analyzing parameters like RMSD, RMSF, Rg, SASA, hydrogen bonds, contact analysis, Gibbs free energy, and cluster analysis. The results revealed stable interactions between the selected metabolites and the target proteins, involving important hydrogen bonds and contacts. The SwissADME server analysis indicated that the metabolites possess fungicide properties, making them effective and safe fungicides with low toxicity to the environment and living beings. Additionally, bioactivity assays confirmed their biological activity as nuclear receptor ligands and enzyme inhibitors. Overall, this study offers valuable insights into potential natural fungicides for combating rice blast disease, with 2-Coumaroylquinic acid, Myricetin, Rosmarinic Acid, and Quercetin standing out as promising and environmentally friendly alternatives to conventional fungicides. These findings have significant implications for developing crop protection strategies and enhancing global food security, particularly in rice-dependent regions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. pH-Responsive Pesticide-Loaded Hollow Mesoporous Silica Nanoparticles with ZnO Quantum Dots as a Gatekeeper for Control of Rice Blast Disease.
- Author
-
Zhao, Yi, Zhang, Yanning, Yan, Yilin, Huang, Zunyao, Zhang, Yuting, Wang, Xiaoli, and Zhou, Nandi
- Subjects
- *
RICE blast disease , *MESOPOROUS silica , *SILICA nanoparticles , *QUANTUM dots , *ZINC oxide , *PESTICIDES - Abstract
Nanotechnology-enabled pesticide delivery systems have been widely studied and show great prospects in modern agriculture. Nanodelivery systems not only achieve the controlled release of agrochemicals but also possess many unique characteristics. This study presents the development of a pH-responsive pesticide nanoformulation utilizing hollow mesoporous silica nanoparticles (HMSNs) as a nanocarrier. The nanocarrier was loaded with the photosensitive pesticide prochloraz (Pro) and then combined with ZnO quantum dots (ZnO QDs) through electrostatic interactions. ZnO QDs serve as both the pH-responsive gatekeeper and the enhancer of the pesticide. The results demonstrate that the prepared nanopesticide exhibits high loading efficiency (24.96%) for Pro. Compared with Pro technical, the degradation rate of Pro loaded in HMSNs@Pro@ZnO QDs was reduced by 26.4% after 24 h ultraviolet (UV) exposure, indicating clearly improved photostability. In a weak acidic environment (pH 5.0), the accumulated release of the nanopesticide after 48 h was 2.67-fold higher than that in a neutral environment. This indicates the excellent pH-responsive characteristic of the nanopesticide. The tracking experiments revealed that HMSNs can be absorbed by rice leaves and subsequently transported to other tissues, indicating their potential for effective systemic distribution and targeted delivery. Furthermore, the bioactivity assays confirmed the fungicidal efficacy of the nanopesticide against rice blast disease. Therefore, the constructed nanopesticide holds great prospect in nanoenabled agriculture, offering a novel strategy to enhance pesticide utilization. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Refinement of rice blast disease resistance QTLs and gene networks through meta-QTL analysis
- Author
-
Basavantraya Navadagi Devanna, Sumali Sucharita, N. C. Sunitha, C. Anilkumar, Pankaj K. Singh, D. Pramesh, Sanghamitra Samantaray, Lambodar Behera, Jawahar Lal Katara, C. Parameswaran, Prachitara Rout, Selvaraj Sabarinathan, Hosahatti Rajashekara, and Tilak Raj Sharma
- Subjects
Candidate genes ,Characterized genes ,Magnaporthe oryzae ,Meta-QTL ,Orthologous genes ,Rice blast ,Medicine ,Science - Abstract
Abstract Rice blast disease is the most devastating disease constraining crop productivity. Vertical resistance to blast disease is widely studied despite its instability. Clusters of genes or QTLs conferring blast resistance that offer durable horizontal resistance are important in resistance breeding. In this study, we aimed to refine the reported QTLs and identify stable meta-QTLs (MQTLs) associated with rice blast resistance. A total of 435 QTLs were used to project 71 MQTLs across all the rice chromosomes. As many as 199 putative rice blast resistance genes were identified within 53 MQTL regions. The genes included 48 characterized resistance gene analogs and related proteins, such as NBS–LRR type, LRR receptor-like kinase, NB-ARC domain, pathogenesis-related TF/ERF domain, elicitor-induced defense and proteins involved in defense signaling. MQTL regions with clusters of RGA were also identified. Fifteen highly significant MQTLs included 29 candidate genes and genes characterized for blast resistance, such as Piz, Nbs-Pi9, pi55-1, pi55-2, Pi3/Pi5-1, Pi3/Pi5-2, Pikh, Pi54, Pik/Pikm/Pikp, Pb1 and Pb2. Furthermore, the candidate genes (42) were associated with differential expression (in silico) in compatible and incompatible reactions upon disease infection. Moreover, nearly half of the genes within the MQTL regions were orthologous to those in O. sativa indica, Z. mays and A. thaliana, which confirmed their significance. The peak markers within three significant MQTLs differentiated blast-resistant and susceptible lines and serve as potential surrogates for the selection of blast-resistant lines. These MQTLs are potential candidates for durable and broad-spectrum rice blast resistance and could be utilized in blast resistance breeding.
- Published
- 2024
- Full Text
- View/download PDF
11. Rice Blast Disease
- Author
-
Nalleli Garcia, Rachel Kalicharan, and Jessie Fernandez Garcia
- Subjects
fungal disease ,blast disease ,Magnaporthe oryzae ,rice ,fungal diseases of plants ,Agriculture (General) ,S1-972 ,Plant culture ,SB1-1110 ,Biology (General) ,QH301-705.5 - Abstract
The purpose of this document is to increase awareness of rice blast disease by describing its lifestyle, symptoms, and current disease mitigation practices. Given that the Everglades Agricultural Area (EAA) plays a vital role in rice cultivation, this publication is also intended for Florida rice growers to be used as a diagnostic field guide in the identification and management of rice blast disease. Written by Nalleli Garcia, Rachel Kalicharan, and Jessie Fernandez Garcia, and published by the UF/IFAS Department of Microbiology and Cell Science, September 2024.
- Published
- 2024
- Full Text
- View/download PDF
12. Field performance assessment of formulated Pseudomonas fluorescens for enhancing plant growth and inducing resistance against rice blast disease
- Author
-
Chakraborty, Shila, Islam, Md. Morshedul, and Khokon, Md. Atiqur Rahman
- Published
- 2024
- Full Text
- View/download PDF
13. Influence of weather parameters on rice blast disease progression in Tamil Nadu, India
- Author
-
B. JOHNSON and T. CHANDRAKUMAR
- Subjects
Blast Disease ,Weather ,Multiple linear regression ,Correlation coefficient ,Agriculture - Abstract
Rice cultivation in Madurai district, Tamil Nadu, spans distinct cropping seasons: Kar (May – Jun), Semi-dry (Jul – Aug), Samba/ Late Samba (Aug – Sep), and Navarai (Dec – Jan), each with unique weather conditions and rice varieties. This study explores the correlation between weather parameters temperature, rainfall, humidity, sunshine hours, and wind speed and rice blast disease severity from 2021 to 2023. Using multiple linear regression with ordinary least squares (OLS), the analysis achieves high predictive accuracy (R² = 0.98). Results show that the maximum temperatures correlated negatively with disease severity (r = -0.869 to -0.892), while rainfall (r = 0.768 to 0.804) and wind speed (r = 0.766 to 0.938) correlated positively during the semi-dry season. Relative humidity exhibits varying impacts across seasons. These findings underscore the importance of tailored disease management strategies, such as targeted fungicidal applications during warmer seasons and optimized water management in others. By elucidating these dynamics, the study enhances understanding of weather-disease interactions, providing actionable insights to optimize disease management and enhance crop resilience in Madurai district.
- Published
- 2024
- Full Text
- View/download PDF
14. Geographic distribution, host preference and phylogenetic relationships among Pyricularia species inciting millet and rice blast disease in India.
- Author
-
Palanna, K. B., Vinaykumar, H. D., Koti, Prasanna S., Jeevan, B., Rajashekara, H., Raveendra, H. R., Patro, T. S. S. K., Ramesh, G. V., Mudalagiriyappa, Rawat, Laxmi, Netam, Prahlad, Ekka, Savita, Rajesha, G., Das, I. K., Chandranayaka, S., Nagaraja, T. E., and Satyavathi, C. Tara
- Subjects
- *
RICE blast disease , *MILLETS , *RAGI , *FOXTAIL millet , *PEARL millet , *SPECIES , *WEEDS - Abstract
Blast disease causes significant damage to millets (pearl millet, finger millet and foxtail millet) and rice in India. This study investigates strains of Pyricularia, the causal agent of blast disease, in rice and millets in India in terms of their diversity, host preferences and phylogeny. One hundred and thirty‐six Pyricularia isolates causing rice and millet blast were collected from 46 locations in India. They displayed morphological diversity irrespective of host or location. All Pyricularia isolates were separated into two major clusters by a multilocus sequence‐based phylogenetic tree, which also demonstrated that most isolates are grouped according to their host associations. In contrast, a few finger millet isolates were found to be grouped with foxtail millet isolates. We explored how Pyricularia isolates behaved when exposed to rice and millets. Finger millet isolates were shown to be pathogenic on a wide variety of millets, whereas rice isolates were only found to infect rice and wheat. The majority of the blast isolates of millets were shown to be pathogenic on common weed species of the millet ecosystem, such as Echinochloa crusgalli, Eleusina indica and Erogrotis gagantica. Our findings emphasize the importance of pathogen surveillance in both cultivated crops and weed hosts, as well as the possible risk of blast fungus infection in Indian millets due to host expansion. Blast disease control programmes in India will be greatly enhanced by the knowledge gained in this study on the diversity and host association of Pyricularia strains. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
15. Influence of weather parameters on rice blast disease progression in Tamil Nadu, India.
- Author
-
JOHNSON, B. and CHANDRAKUMAR, T.
- Subjects
RICE blast disease ,WEATHER ,HUMIDITY ,WIND speed ,RAINFALL - Abstract
Rice cultivation in Madurai district, Tamil Nadu, spans distinct cropping seasons: Kar (May -- Jun), Semi-dry (Jul - Aug), Samba/ Late Samba (Aug - Sep), and Navarai (Dec - Jan), each with unique weather conditions and rice varieties. This study explores the correlation between weather parameters temperature, rainfall, humidity, sunshine hours, and wind speed and rice blast disease severity from 2021 to 2023. Using multiple linear regression with ordinary least squares (OLS), the analysis achieves high predictive accuracy (R² = 0.98). Results show that the maximum temperatures correlated negatively with disease severity (r = -0.869 to -0.892), while rainfall (r = 0.768 to 0.804) and wind speed (r = 0.766 to 0.938) correlated positively during the semi-dry season. Relative humidity exhibits varying impacts across seasons. These findings underscore the importance of tailored disease management strategies, such as targeted fungicidal applications during warmer seasons and optimized water management in others. By elucidating these dynamics, the study enhances understanding of weather-disease interactions, providing actionable insights to optimize disease management and enhance crop resilience in Madurai district. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
16. Exploring fungal endophytes as biocontrol agents against rice blast disease
- Author
-
Mohammad Reza Safari Motlagh, Dariusz Kulus, Behzad Kaviani, and Hadi Habibollahi
- Subjects
agriculture ,antagonistic fungi ,disease rating ,fungal diseases ,mycelial growth inhibition ,volatile metabolites ,Agriculture (General) ,S1-972 - Abstract
The rice blast disease caused by Pyricularia oryzae Cavara is among the most severe plant diseases in the world. The utilization of natural antagonists, such as fungal endophytes, can be a good method to control the spread of this disease. In the present study, 68 fungal isolates were obtained from 160 rice samples collected in Guilan province fields, Iran. Morphological and molecular characteristics were used for the identification of these fungi. According to the results, the fungal isolates were Alternaria citri (Penz.) Mussat, Alternaria infectoria Simmons, Aspergillus fumigatus Fresen, Fusarium verticillioides (Sacc.) Nirenberg, Trichoderma harzianum Rifai, Trichoderma virens (Mill., Giddens & Foster) Arx, Trichoderma viride Pers., Ulocladium consortiale (Thum) Simmons, and Pyricularia oryzae . Eight isolates that did not cause any plant disease were selected for biocontrol studies in vitro . The dual culture, culture filtrate, slide culture, and volatile metabolites methods were used in search of natural endophytic antagonists of Pyricularia oryzae . It was found that T. harzianum, T. virens, T. viride, A. fumigatus , and A. citri isolates caused the highest percentage of mycelial growth inhibition of P. oryzae in vitro . In the greenhouse experiments, all of the studied isolates reduced the P. oryzae disease rating, with T. harzianum isolate being the most effective antagonist (27.36% reduction in the disease rating), followed by T. virens, T. viride , and A. fumigatus (20.75%, 17.92%, and 16.98%, respectively). Furthermore, in greenhouse conditions, these antagonistic fungi increased the height, fresh weight, and dry weight of plants. The results of this research showed that there are various fungi in the natural flora of rice plants that possess potential biological properties against rice blast disease that can be implemented into practice.
- Published
- 2024
- Full Text
- View/download PDF
17. Progress in the Management of Rice Blast Disease: The Role of Avirulence and Resistance Genes through Gene-for-Gene Interactions.
- Author
-
Younas, Muhammad Usama, Ahmad, Irshad, Qasim, Muhammad, Ijaz, Zainab, Rajput, Nimra, Parveen Memon, Saima, UL Zaman, Waqar, Jiang, Xiaohong, Zhang, Yi, and Zuo, Shimin
- Subjects
- *
RICE blast disease , *LOCUS (Genetics) , *MOLECULAR cloning , *CROPS , *PYRICULARIA oryzae - Abstract
Rice is a vital component in the diets of many people worldwide, supplying necessary calories for subsistence. Nevertheless, the yield of this crucial agricultural crop is consistently hindered by a range of biotic stresses. Out of these, rice blast, claused mainly by the fungus Magnaporthe oryzae, poses a significant menace to worldwide rice cultivation as well as yield in recent years. The consequences are particularly crucial given the current climate change challenges. In recent decades, substantial progress has been achieved in the development of efficient ways to manage rice blast disease. These procedures entail using a variety of rice genetic resources to find, map, clone, and functionally validate individual resistance (R) genes and quantitative trait loci (QTLs) that provide long-lasting resistance to rice blast disease. Moreover, the replication and practical confirmation of homologous avirulence (Avr) genes in various M. oryzae strains have been crucial in comprehending the fundamental molecular mechanisms of host–pathogen interactions. This article offers a thorough examination of the cloning and functional verification of different R genes and QTLs linked to resistance against rice blast disease. The complex interplay between R–Avr pairings, which contributes to the development of resistance against rice blast throughout a wide range, is thoroughly explained. Finally, this study explores the most recent progress in next-generation sequencing (NGS) and genome editing technologies (GETs), examining their potential uses in improving the treatment of rice blast disease. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
18. Unveiling the Roles of LncRNA MOIRAs in Rice Blast Disease Resistance.
- Author
-
Liu, Qing, Xue, Jiao, Zhang, Lanlan, Jiang, Liqun, and Li, Chen
- Subjects
- *
RICE blast disease , *DISEASE resistance of plants , *PYRICULARIA oryzae , *LINCRNA , *CROP yields - Abstract
Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, is a major threat to rice production worldwide. This study investigates the role of long non-coding RNAs (lncRNAs) in rice's response to this destructive disease, with a focus on their impacts on disease resistance and yield traits. Three specific lncRNAs coded by M. oryzae infection-responsive lncRNAs (MOIRAs), MOIRA1, MOIRA2, and MOIRA3, were identified as key regulators of rice's response to M. oryzae infection. Strikingly, when MOIRA1 and MOIRA2 were overexpressed, they exhibited a dual function: they increased rice's susceptibility to blast fungus, indicating a negative role in disease resistance, while simultaneously enhancing tiller numbers and single-plant yield, with no adverse effects on other yield-related traits. This unexpected improvement in productivity suggests the possibility of overcoming the traditional trade-off between disease resistance and crop yield. These findings provide a novel perspective on crop enhancement, offering a promising solution to global food security challenges by developing rice varieties that effectively balance disease resistance and increased productivity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
19. The role of circadian entrainment in rice blast disease
- Author
-
Griffin, Ciaran
- Subjects
Plant pathology ,Circadian rhythms ,Fungal physiology ,Image processing ,Imaging ,Microscopy - Abstract
A circadian clock is present in some capacity in almost all forms of life, and is useful for a wide array of traits, but crucially allows organisms to predict future conditions and adapt their behaviour to synchronise with, and thrive under, their dynamic environment. Accordingly, plant environmental stress responses are gated in a circadian manner, including that for pathogenic defence and immunity. Comparatively less work has been carried out on the plant pathogens, but there are increasing reports of pathogens capable of rhythmically altering their behaviour and virulence-related traits. Magnaporthe oryzae, the fungal pathogen responsible for the destruction of enough rice to feed at least 60 M people annually, has been shown to possess some circadian clock components, and based on bioinformatic analyses, likely contains all the core, accessory, and circadian-associated genes. M. oryzae displays a conidial banding pattern, reminiscent of the model clock species, N. crassa, and (after sufficient entrainment) this pattern can continue to occur under free running conditions for a number of days, with a period of approximately 24 h. This rhythm is also presented on a range of nutrient-rich and poor media, suggesting a nutritionally-compensated circadian rhythm in M. oryzae. This onset of conidial banding is partially determined by the presence of secreted metabolites, the sensation of which is facilitated by the circadian clock, predominantly via WC2. The entraining light conditions that M. oryzae is exposed to can significantly alter its vegetative growth, conidiation and conidial development, and even pathogenicity. Further, inoculation timing (dawn or dusk) plays a role in both the virulence of M. oryzae, and in the susceptibility of the plant host, seemingly in a species-by-species manner, where rice is most susceptible at dawn, and barley most susceptible at dusk. For M. oryzae, pre-inoculation entrainment to darkness predominantly favours dawn inoculations, and those exposed to prolonged periods of light prefer dusk inoculation. Upon mutation of the core clock genes, WC2 and FRQ, vegetative growth, conidiation and conidial development, photoadaptation, and pathogenicity were all significantly altered compared to the wild type, suggesting an important role of the clock in the general fitness of M. oryzae. This work discusses how entraining light cycles and the circadian clock impacts the growth, development, conidiation, virulence, and ultimate severity in the economically important rice blast disease.
- Published
- 2023
20. Key transcription factors required for outburst of rice blast disease in Magnaporthe oryzae
- Author
-
Qing Wang, Zhicheng Huang, Irshad Ali Khan, Yan Li, Jing Wang, Jiaoyu Wang, Xiao-Hong Liu, Fucheng Lin, and Jianping Lu
- Subjects
Appressorium formation ,Conidiation ,Invasive growth ,Carbohydrate metabolism ,Lipid metabolism ,Melanin synthesis ,Plant culture ,SB1-1110 - Abstract
Abstract Rice blast is a serious threat to the safe production of grain crops such as rice and wheat. Sporulation, appressorium formation, and invasive growth of Magnaporthe oryzae are the key stages of the development and spread of rice blast epidemics. M. oryzae is a hemibiotrophic fungus that undergoes changes in available carbon sources during the infection cycle. Lipid is a major storage for M. oryzae spores and a major carbon source used in glycerol synthesis and turgor pressure generation in appressoria. The formation of a dense cell wall melanin layer is necessary for an appressorium to produce turgor and to be pathogenic. The plant cell wall is an important carbon source during the infection stage of M. oryzae. Transcription factors regulate gene expression in fungi and are key intermediates between the reception of external environmental signals and the control of development and pathogenicity in M. oryzae. The disease cycle of M. oryzae is controlled by some key transcription factors, such as sporulation by Cos1 and Hox2, appressorium formation by Sfl1, Hox7, and Vrf1, invasive growth by Mst12 and Mig1, and resistance to host basal immunity by Ap1 and Atf1. This review focuses on describing the key transcription factors of M. oryzae that regulate sporulation, appressorium formation, invasive growth, lipid metabolism, carbohydrate metabolism, melanin synthesis, oxidative response, and host basal immunity, as well as the working mechanism of the transcription factors.
- Published
- 2024
- Full Text
- View/download PDF
21. A smart dual-responsive nanoplatform for delivery of prochloraz for the control of rice blast disease
- Author
-
Zhang, Zhaoyang, Li, Donglin, Yu, Chang, Li, Jiaqing, Sun, Dan, Wang, Jiayin, Mmby, Mohamed, Li, Jianhong, You, Hong, and He, Shun
- Published
- 2024
- Full Text
- View/download PDF
22. An extension of mathematical model for severity of rice blast disease
- Author
-
Saharat Tabonglek, Amir Khan, and Usa Wannasingha Humphries
- Subjects
disease severity ,epidemic model ,plant disease ,rice blast disease ,spore dispersal ,Mathematics ,QA1-939 - Abstract
This paper aims to extend the spore dispersal model to the Healthy-Latent-Infectious-Removed (HLIR) epidemic model for assessing the severity of rice blast disease. The model was solved by the Finite Difference Method (FDM). The results of the model were compared to data from the Prachinburi Rice Research Center (PRRC) on the severity of rice blast disease. Because of a small error, the comparison results showed good agreement between the PRRC data and the simulation by looking at the value of Willmott's index of agreement (d). The first bed d was 0.7166, while the second bed d was 0.6421, indicating the model's performance. Furthermore, the optimal parameter, the fraction of spores deposited on the crop, was determined to be 0.173 and 0.016 for beds 1 and 2, respectively. The model can simulate and analyze rice blast outbreaks for educational purposes in future preparedness planning.
- Published
- 2023
- Full Text
- View/download PDF
23. Novel hybrid ARIMA–BiLSTM model for forecasting of rice blast disease outbreaks for sustainable rice production
- Author
-
Varsha, M., Poornima, B., Pavan Kumar, M. P., and Basavarajappa, S.
- Published
- 2023
- Full Text
- View/download PDF
24. Field performance assessment of formulated Pseudomonas fluorescens for enhancing plant growth and inducing resistance against rice blast disease
- Author
-
Shila Chakraborty, Md. Morshedul Islam, and Md. Atiqur Rahman Khokon
- Subjects
Disease incidence and severity ,Formulation ,H2O2 ,Phenol ,Pseudomonas fluorescens ,Science (General) ,Q1-390 - Abstract
To control blast disease in the rice field under natural conditions, three antagonistic isolates of rhizospheric P. fluorescens were formulated in talc, kaolinite, PVP, and vegetable oil using an RCBD with three replications. At various phases of the rice plants' growth, all of the products markedly accelerated plant growth and yield-contributing characteristics. Pf-8, compounded in talc (5 %) and vegetable oil (2 %) among the three isolates of P. fluorescens, significantly elevated vegetative and yield parameters with higher (2.11 and 2.08) benefit-cost ratios, respectively. At 90 days after transplanting (DAT), T11 (5 % Pf-8 Talc) and T13 (2 % Pf-8 Vegetable oil) showed a significant reduction in blast incidence (76.81 %, 75.45 %) and severity (71.57 %, 69.82 %), with the largest populations of P. fluorescens (9.60 × 1010 and 9.51 × 1010) in the rhizosphere. Moreover, a significantly increased level of phenol and hydrogen peroxide (H2O2) was found in Pseudomonas-treated leaves (Pf-8) at 30, 60, and 90 DAT indicating a strong relationship with rice blast disease reduction. Further, blast incidence and severity showed a negative correlation with vegetative parameters, yield parameters, phenol, and H2O2. Thus, it may be claimed that using formulated P. fluorescens (Pf-8) to manage rice blast in the field could be an alternate strategy to using chemicals.
- Published
- 2024
- Full Text
- View/download PDF
25. Bacillus amyloliquefaciens D203 ameliorates rice growth and resistance to rice blast disease
- Author
-
Francis Mirara, Daniel Kwadjo Dzidzienyo, and Maina Mwangi
- Subjects
Bacillus amyloliquefaciens ,magnaporthe oryzae ,germination ,seed coating ,disease management ,Manuel Tejada, Universidad de Sevilla, Spain ,Agriculture ,Food processing and manufacture ,TP368-456 - Abstract
This study evaluated the effects of Bacillus amyloliquefaciens D203 encapsulated in alginate-bentonite coating enriched with titanium nanoparticles on rice germination and Magnaporthe oryzae infection. Seed coating was achieved using the ionic gelation/extrusion method with modifications. The study investigated various concentrations of strain D203 on biomass and bacterial release from the beads, along with its influence on detached leaves. Results showed that treatment with a strain D203 cell suspension (2 × 107 CFU/ml) significantly enhanced root and shoot lengths, despite a slightly lower germination rate (90%) compared to the control. Treatment with a concentration of 1 × 107 proved to be optimum for growth because it maintained germination at 100% and boosted biomass growth of shoot and root by 49.57% and 16.44%, respectively. Colonization by strain D203, explored through seed coating and subsequent resuspension from roots and media, peaked in roots, indicating preferential root zone inhabitation. In vitro studies demonstrated strain D203’s efficacy against M. oryzae at 21 and 40 days, with the 1 × 107 CFU/ml spray significantly reducing disease symptoms. Strain D203 exhibited a preventive effect, especially in early growth stages. The encapsulated form of strain D203 had a control effect comparable to 40% Hexaconazole but less effective than 75% Tricyclazole. Uncoated and encapsulated B. amyloliquefaciens treatments significantly improved sterile rice plant growth over 21 days, with the encapsulated bead treatment surpassing both controls and free cell treatments by days 14 and 21, despite slower initial growth. Encapsulation presents a promising alternative, potentially matching the effectiveness of chemical fungicides, emphasizing the importance of environmentally friendly alternatives in sustainable disease management strategies.
- Published
- 2024
- Full Text
- View/download PDF
26. Enhancing defense against rice blast disease: Unveiling the role of leaf endophytic firmicutes in antifungal antibiosis and induced systemic resistance
- Author
-
Velmurugan, Shanmugam, Ashajyothi, Mushineni, Charishma, Krishnappa, Kumar, Shanu, Balamurugan, Alexander, Javed, Mohammed, Karwa, Sourabh, Prakash, Ganesan, Subramanian, S., Gogoi, Robin, Eke, Pierre, and Kumar, Aundy
- Published
- 2023
- Full Text
- View/download PDF
27. A Review of Conventional and Molecular Approaches for the Management of Rice Blast Disease
- Author
-
Maurya, Rashmi, primary, Singh, Munna, additional, and Srivastava, Deepti, additional
- Published
- 2023
- Full Text
- View/download PDF
28. Binding of stress-responsive OsWRKY proteins through WRKYGQK heptapeptide residue with the promoter region of two rice blast disease resistance genes Pi2 and Pi54 is important for development of blast resistance.
- Author
-
Meher, Jhumishree, Sarkar, Ankita, and Sarma, Birinchi Kumar
- Subjects
- *
RICE blast disease , *ZINC-finger proteins , *PROMOTERS (Genetics) , *MOLECULAR docking , *GENES , *PROTEINS - Abstract
Molecular docking was done to investigate the interactions between five differentially expressed rice WRKY proteins when challenged with the rice blast disease caused by Magnaporthe oryzae and drought stresses applied either individually or overlapped, with the promoter region of two blast resistance genes (Pi2 and Pi54). Molecular docking was performed using the HDOCK server. Initially, the homology models for each of the five rice WRKY proteins were prepared using I-TASSER server, and then the secondary structure as well as the DNA-binding pockets were predicted using PSIPRED and BindUP servers, respectively. The molecular docking study revealed a differential binding pattern of the rice WRKYs with the two blast resistance genes. The WRKY proteins (OsWRKY88 and OsWRKY102), whose transcript levels decrease when drought and blast stresses are overlapped, interact with the two resistance genes mostly involving the residues of the zinc finger structure. On the other hand, the WRKY proteins (OsWRKY53-1 and OsWRKY113), whose transcript levels did not reduce significantly when challenged by drought and blast overlapped condition compared to individual treatment of blast, interact mostly involving the residues of the conserved WRKYGQK heptapeptide sequence. Interestingly, the protein OsWRKY74 whose transcript levels are unaffected in both individual and overlapped stresses, interacts with both the blast resistance genes involving few residues of both WRKYGQK heptapeptide and the zinc finger structure. The findings thus indicate that the interaction of OsWRKY proteins involving the conserved WRKYGQK heptapeptide sequence with the blast resistance genes Pi2 and Pi54 is important to mitigate the blast challenge in rice even during overlapping challenges of drought. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
29. Effect of weather parameters on the development and progression of rice blast disease in Jammu plains
- Author
-
Pandit, Devanshi, Singh, A. K., Singh, S. K., and Singh, V. B.
- Published
- 2023
- Full Text
- View/download PDF
30. Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease
- Author
-
Suhas Gorakh Karkute, Vishesh Kumar, Mohd Tasleem, Dwijesh Chandra Mishra, Krishna Kumar Chaturvedi, Anil Rai, Amitha Mithra Sevanthi, Kishor Gaikwad, Tilak Raj Sharma, and Amolkumar U. Solanke
- Subjects
von Willebrand factor A ,biotic stress ,abiotic stress ,rice blast disease ,Magnaporthe oryzae ,Plant culture ,SB1-1110 - Abstract
von Willebrand factor A (vWA) genes are well characterized in humans except for few BONZAI genes, but the vWA genes are least explored in plants. Considering the novelty and vital role of vWA genes, this study aimed at characterization of vWA superfamily in rice. Rice genome was found to have 40 vWA genes distributed across all the 12 chromosomes, and 20 of the 40 vWA genes were unique while the remaining shared large fragment similarities with each other, indicating gene duplication. In addition to vWA domain, vWA proteins possess other different motifs or domains, such as ubiquitin interacting motif in protein degradation pathway, and RING finger in protein-protein interaction. Expression analysis of vWA genes in available expression data suggested that they probably function in biotic and abiotic stress responses including hormonal response and signaling. The frequency of transposon elements in the entire 3K rice germplasm was negligible except for 9 vWA genes, indicating the importance of these genes in rice. Structural and functional diversities showed that the vWA genes in a blast-resistant rice variety Tetep had huge variations compared to blast-susceptible rice varieties HP2216 and Nipponbare. qRT-PCR analysis of vWA genes in Magnaporthe oryzae infected rice tissues indicated OsvWA9, OsvWA36, OsvWA37 and OsvWA18 as the optimal candidate genes for disease resistance. This is the first attempt to characterize vWA gene family in plant species.
- Published
- 2022
- Full Text
- View/download PDF
31. Genome editing interventions to combat rice blast disease
- Author
-
Khanale, Vaishali, Bhattacharya, Anjanabha, Prashar, Mohinder, and Char, Bharat
- Published
- 2023
- Full Text
- View/download PDF
32. Application of jasmonic acid at the stage of visible brown necrotic spots in Magnaporthe oryzae infection as a novel and environment-friendly control strategy for rice blast disease
- Author
-
Wang, Yunfeng, Duan, Guihua, Li, Chunqin, Ma, Xiaoqing, and Yang, Jing
- Published
- 2021
- Full Text
- View/download PDF
33. Isolation of Bacillus siamensis B-612, a Strain That Is Resistant to Rice Blast Disease and an Investigation of the Mechanisms Responsible for Suppressing Rice Blast Fungus.
- Author
-
Yang, Yanmei, Zhang, Yifan, Zhang, Luyi, Zhou, Zhanmei, Zhang, Jia, Yang, Jinchang, Gao, Xiaoling, Chen, Rongjun, Huang, Zhengjian, Xu, Zhengjun, and Li, Lihua
- Subjects
- *
RICE blast disease , *BACILLUS (Bacteria) , *FUNGI , *FIELD research , *RICE , *INTERNAL auditing - Abstract
Rice yield can be significantly impacted by rice blast disease. In this investigation, an endophytic strain of Bacillus siamensis that exhibited a potent inhibitory effect on the growth of rice blast was isolated from healthy cauliflower leaves. 16S rDNA gene sequence analysis showed that it belongs to the genus Bacillus siamensis. Using the rice OsActin gene as an internal control, we analyzed the expression levels of genes related to the defense response of rice. Analysis showed that the expression levels of genes related to the defense response in rice were significantly upregulated 48 h after treatment. In addition, peroxidase (POD) activity gradually increased after treatment with B-612 fermentation solution and peaked 48 h after inoculation. These findings clearly demonstrated that the 1-butanol crude extract of B-612 retarded and inhibited conidial germination as well as the development of appressorium. The results of field experiments showed that treatment with B-612 fermentation solution and B-612 bacterial solution significantly reduced the severity of the disease before the seedling stage of Lijiangxintuan (LTH) was infected with rice blast. Future studies will focus on exploring whether Bacillus siamensis B-612 produces new lipopeptides and will apply proteomic and transcriptomic approaches to investigate the signaling pathways involved in its antimicrobial effects. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
34. Antifungal plant flavonoids identified in silico with potential to control rice blast disease caused by Magnaporthe oryzae.
- Author
-
Abu Tayab Moin, Tanjin Barketullah Robin, Rajesh B Patil, Nurul Amin Rani, Anindita Ash Prome, Tahsin Islam Sakif, Mohabbat Hossain, Dil Umme Salma Chowdhury, Shah Samiur Rashid, A K M Moniruzzaman Mollah, Saiful Islam, Mohammad Helal Uddin, Mohammad Khalequzzaman, Tofazzal Islam, and Nazneen Naher Islam
- Subjects
Medicine ,Science - Abstract
Rice blast disease, caused by the fungus Magnaporthe oryzae, poses a severe threat to rice production, particularly in Asia where rice is a staple food. Concerns over fungicide resistance and environmental impact have sparked interest in exploring natural fungicides as potential alternatives. This study aimed to identify highly potent natural fungicides against M. oryzae to combat rice blast disease, using advanced molecular dynamics techniques. Four key proteins (CATALASE PEROXIDASES 2, HYBRID PKS-NRPS SYNTHETASE TAS1, MANGANESE LIPOXYGENASE, and PRE-MRNA-SPLICING FACTOR CEF1) involved in M. oryzae's infection process were identified. A list of 30 plant metabolites with documented antifungal properties was compiled for evaluation as potential fungicides. Molecular docking studies revealed that 2-Coumaroylquinic acid, Myricetin, Rosmarinic Acid, and Quercetin exhibited superior binding affinities compared to reference fungicides (Azoxystrobin and Tricyclazole). High throughput molecular dynamics simulations were performed, analyzing parameters like RMSD, RMSF, Rg, SASA, hydrogen bonds, contact analysis, Gibbs free energy, and cluster analysis. The results revealed stable interactions between the selected metabolites and the target proteins, involving important hydrogen bonds and contacts. The SwissADME server analysis indicated that the metabolites possess fungicide properties, making them effective and safe fungicides with low toxicity to the environment and living beings. Additionally, bioactivity assays confirmed their biological activity as nuclear receptor ligands and enzyme inhibitors. Overall, this study offers valuable insights into potential natural fungicides for combating rice blast disease, with 2-Coumaroylquinic acid, Myricetin, Rosmarinic Acid, and Quercetin standing out as promising and environmentally friendly alternatives to conventional fungicides. These findings have significant implications for developing crop protection strategies and enhancing global food security, particularly in rice-dependent regions.
- Published
- 2024
- Full Text
- View/download PDF
35. Predicting rice blast disease: machine learning versus process based models
- Author
-
Nettleton, David F., Katsantonis, Dimitrios, Kalaitzidis, Argyris, Sarafijanovic-Djukic, Natasa, Puigdollers, Pau, and Confalonieri, Roberto
- Subjects
Quantitative Biology - Quantitative Methods ,Computer Science - Machine Learning ,Statistics - Machine Learning - Abstract
Rice is the second most important cereal crop worldwide, and the first in terms of number of people who depend on it as a major staple food. Rice blast disease is the most important biotic constraint of rice cultivation causing each year millions of dollars of losses. Despite the efforts for breeding new resistant varieties, agricultural practices and chemical control are still the most important methods for disease management. Thus, rice blast forecasting is a primary tool to support rice growers in controlling the disease. In this study, we compared four models for predicting rice blast disease, two operational process-based models (Yoshino and WARM) and two approaches based on machine learning algorithms (M5Rules and RNN), the former inducing a rule-based model and the latter building a neural network. In situ telemetry is important to obtain quality in-field data for predictive models and this was a key aspect of the RICE-GUARD project on which this study is based. According to the authors, this is the first time process-based and machine learning modelling approaches for supporting plant disease management are compared.
- Published
- 2020
- Full Text
- View/download PDF
36. Evaluation of Medicinal Plant Extracts for Rice Blast Disease Control.
- Author
-
Yanping, Tan, Shiqi, Deng, Yonghua, Qin, Xin, Xu, You, Yu, Liu, Cui, Chuntai, Wang, Changjie, Jiang, and Xinqiong, Liu
- Subjects
RICE blast disease ,PLANT extracts ,PREVENTIVE medicine - Published
- 2023
- Full Text
- View/download PDF
37. A deep convolutional neural network-based wavelength selection method for spectral characteristics of rice blast disease
- Author
-
Feng, Shuai, Zhao, Dongxue, Guan, Qiang, Li, Jinpeng, Liu, Ziyang, Jin, Zhongyu, Li, Guangming, and Xu, Tongyu
- Published
- 2022
- Full Text
- View/download PDF
38. Approaches to Reduce Rice Blast Disease Using Knowledge from Host Resistance and Pathogen Pathogenicity.
- Author
-
Younas, Muhammad Usama, Wang, Guanda, Du, Haibo, Zhang, Yi, Ahmad, Irshad, Rajput, Nimra, Li, Mingyou, Feng, Zhiming, Hu, Keming, Khan, Nasr Ullah, Xie, Wenya, Qasim, Muhammad, Chen, Zongxiang, and Zuo, Shimin
- Subjects
- *
DISEASE resistance of plants , *RICE blast disease , *RICE diseases & pests , *LOCUS (Genetics) , *CROP yields - Abstract
Rice is one of the staple foods for the majority of the global population that depends directly or indirectly on it. The yield of this important crop is constantly challenged by various biotic stresses. Rice blast, caused by Magnaporthe oryzae (M. oryzae), is a devastating rice disease causing severe yield losses annually and threatening rice production globally. The development of a resistant variety is one of the most effective and economical approaches to control rice blast. Researchers in the past few decades have witnessed the characterization of several qualitative resistance (R) and quantitative resistance (qR) genes to blast disease as well as several avirulence (Avr) genes from the pathogen. These provide great help for either breeders to develop a resistant variety or pathologists to monitor the dynamics of pathogenic isolates, and ultimately to control the disease. Here, we summarize the current status of the isolation of R, qR and Avr genes in the rice–M. oryzae interaction system, and review the progresses and problems of these genes utilized in practice for reducing rice blast disease. Research perspectives towards better managing blast disease by developing a broad-spectrum and durable blast resistance variety and new fungicides are also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
39. Importance of OsRac1 and RAI1 in signalling of nucleotide-binding site leucine-rich repeat protein-mediated resistance to rice blast disease
- Author
-
Zhou, Zhuangzhi, Pang, Zhiqian, Zhao, Shengli, Zhang, Lingli, Lv, Qiming, Yin, Dedong, Li, Dayong, Liu, Xue, Zhao, Xianfeng, Li, Xiaobing, Wang, Wenming, and Zhu, Lihuang
- Published
- 2019
40. Osa-miR398b boosts H₂O₂ production and rice blast disease-resistance via multiple superoxide dismutases
- Author
-
Li, Yan, Cao, Xiao-Long, Zhu, Yong, Yang, Xue-Mei, Zhang, Kai-Ni, Xiao, Zhi-Yuan, Wang, He, Zhao, Jing-Hao, Zhang, Ling-Li, Li, Guo-Bang, Zheng, Ya-Ping, Fan, Jing, Wang, Jing, Chen, Xiao-Qiong, Wu, Xian-Jun, Zhao, Ji-Qun, Dong, Oliver Xiaoou, Chen, Xue-Wei, Chern, Mawsheng, and Wang, Wen-Ming
- Published
- 2019
41. Weather-based rice blast disease forecasting
- Author
-
Sriwanna, Kittakorn
- Published
- 2022
- Full Text
- View/download PDF
42. Determination of the Efficacy of Different Spraying and Planting Methods on Management of Rice Blast Disease (Pyricularia oryzae) in the Mediterranean Region
- Author
-
Efkan AKÇALI and Şener KURT
- Subjects
rice ,rice blast disease ,disease management ,Agriculture (General) ,S1-972 - Abstract
Rice is one of the important food sources in human nutrition. Rice blast disease caused Pyricularia oryzae is the most destructive disease which is seen in rice growing. The aim of this study was to investigate the effect of different spraying and planting methods on disease severity and yield in rice fields in the Mediterranean Region. Field trials were conducted onto rice fields which were grown Edirne variety in the Tabaklar village, Yüregir, Adana province and Sökün village, Silifke, Mersin province in 2011 and 2012, respectively. As a result of this study, the use of treated seeds and seedlings reduced the severity of the disease. The lowest disease severity was found as 6.1% and 9.3% where it was used by years. However, the disease severity was also recorded as the lowest value by 6.6% and 4.6%, respectively through foliar spraying in the plot where the seedlings were performed from treated seeds by years. No impact on different spraying and planting methods on the yield was found.
- Published
- 2021
- Full Text
- View/download PDF
43. Rice Blast Disease Detection Using CNN Models and DCGAN
- Author
-
Al Munem, Abdullah, primary, Maha, Lamyea Tasneem, additional, Haque, Rafid Mahmud, additional, Safa, Noor Fabi Shah, additional, Khan, Mozammel H. A., additional, and Khan, Mohammad Ashik Iqbal, additional
- Published
- 2023
- Full Text
- View/download PDF
44. Cell wall polysaccharides of endophytic Pseudomonas putida elicit defense against rice blast disease.
- Author
-
Ashajyothi, Mushineni, Balamurugan, Alexander, Patel, Asharani, Krishnappa, Charishma, Kumar, Rajesh, and Kumar, Aundy
- Subjects
- *
RICE blast disease , *PSEUDOMONAS putida , *MICROBIAL polysaccharides , *POLYSACCHARIDES , *LASER microscopy - Abstract
Aims To characterize the functional role of extracellular polysaccharides and lipopolysaccharide (LPS) extracted from endophytic Pseudomonas putida BP25 (PpBP25) against rice blast. Methods and Results We profiled the transcriptome of endobacterized rice seedlings using RNA-seq. Fluorescence imaging of interaction between Magnaporthe :: gfp and P. putida :: mCherry was performed on rice phylloplane using confocal laser scanning microscopy (CLSM). Microbial polysaccharides, exopolysaccharide (EPS), and LPS extracted from PpBP25 were characterized using Fourier-transform infrared-spectroscopic analysis (FTIR). Biochemical assays and gene expression analysis were conducted on EPS- and LPS-treated rice seedlings. A detached-leaf assay was designed to test the blasticidal-effect of bacterial-endophyte, EPS, and LPS on rice phylloplane. PpBP25 elicited defense in rice with a consequently altered seedling phenotype. Rice cultivar, Pusa Basmati-1, colonized by PpBP25 showed an altered transcriptome profile displaying a total of 110-downregulated and 68-upregulated genes (P < 0.005) representing growth/development and defense pathways, respectively. CLSM of PpBP25 bacterized phylloplane showed reduced conidial-germination and mycelial-biomass of Magnaporthe oryzae. To decipher the elicitor role of polysaccharides, we purified and characterized EPS and LPS using FTIR. Rice treated with the EPS and LPS showed root-growth inhibition the phenotype of MAMP-triggered immunity. While the EPS showed blast suppressive activity at 1–20 mg mL−1 (79.80%–86.87% reduction over control), the LPS exhibited 78.0%–79.8% reduction at 20–200 μg mL−1on rice. Polysaccharides treated seedling showed elevated activities of peroxidase and polyphenol-oxidase activities, and total-phenols content. Treated plantlets showed up regulation of OsPR1.1,OsPR3, OsGLP3-3,OsZFP179 , and Oshox24 as well as downregulation of OsACS6. Conclusions We showed that P. putida Bp25 and its cell wall-associated polysaccharides could elicit defense against rice blast [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
45. pH-Responsive Pesticide-Loaded Hollow Mesoporous Silica Nanoparticles with ZnO Quantum Dots as a Gatekeeper for Control of Rice Blast Disease
- Author
-
Yi Zhao, Yanning Zhang, Yilin Yan, Zunyao Huang, Yuting Zhang, Xiaoli Wang, and Nandi Zhou
- Subjects
nanopesticide ,hollow mesoporous silica ,stimuli responsive ,Magnaporthe oryzae ,prochloraz ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Nanotechnology-enabled pesticide delivery systems have been widely studied and show great prospects in modern agriculture. Nanodelivery systems not only achieve the controlled release of agrochemicals but also possess many unique characteristics. This study presents the development of a pH-responsive pesticide nanoformulation utilizing hollow mesoporous silica nanoparticles (HMSNs) as a nanocarrier. The nanocarrier was loaded with the photosensitive pesticide prochloraz (Pro) and then combined with ZnO quantum dots (ZnO QDs) through electrostatic interactions. ZnO QDs serve as both the pH-responsive gatekeeper and the enhancer of the pesticide. The results demonstrate that the prepared nanopesticide exhibits high loading efficiency (24.96%) for Pro. Compared with Pro technical, the degradation rate of Pro loaded in HMSNs@Pro@ZnO QDs was reduced by 26.4% after 24 h ultraviolet (UV) exposure, indicating clearly improved photostability. In a weak acidic environment (pH 5.0), the accumulated release of the nanopesticide after 48 h was 2.67-fold higher than that in a neutral environment. This indicates the excellent pH-responsive characteristic of the nanopesticide. The tracking experiments revealed that HMSNs can be absorbed by rice leaves and subsequently transported to other tissues, indicating their potential for effective systemic distribution and targeted delivery. Furthermore, the bioactivity assays confirmed the fungicidal efficacy of the nanopesticide against rice blast disease. Therefore, the constructed nanopesticide holds great prospect in nanoenabled agriculture, offering a novel strategy to enhance pesticide utilization.
- Published
- 2024
- Full Text
- View/download PDF
46. Genome-Wide Analysis of von Willebrand Factor A Gene Family in Rice for Its Role in Imparting Biotic Stress Resistance with Emphasis on Rice Blast Disease.
- Author
-
Karkute, Suhas Gorakh, Kumar, Vishesh, Tasleem, Mohd, Mishra, Dwijesh Chandra, Chaturvedi, Krishna Kumar, Rai, Anil, Sevanthi, Amitha Mithra, Gaikwad, Kishor, Sharma, Tilak Raj, and Solanke, Amolkumar U.
- Subjects
RICE blast disease ,VON Willebrand factor ,GENE families ,RICE ,PROTEOLYSIS ,CHROMOSOME duplication ,PLANT genes - Abstract
von Willebrand factor A (vWA) genes are well characterized in humans except for few BONZAI genes, but the vWA genes are least explored in plants. Considering the novelty and vital role of vWA genes, this study aimed at characterization of vWA superfamily in rice. Rice genome was found to have 40 vWA genes distributed across all the 12 chromosomes, and 20 of the 40 vWA genes were unique while the remaining shared large fragment similarities with each other, indicating gene duplication. In addition to vWA domain, vWA proteins possess other different motifs or domains, such as ubiquitin interacting motif in protein degradation pathway, and RING finger in protein-protein interaction. Expression analysis of vWA genes in available expression data suggested that they probably function in biotic and abiotic stress responses including hormonal response and signaling. The frequency of transposon elements in the entire 3K rice germplasm was negligible except for 9 vWA genes, indicating the importance of these genes in rice. Structural and functional diversities showed that the vWA genes in a blast-resistant rice variety Tetep had huge variations compared to blast-susceptible rice varieties HP2216 and Nipponbare. qRT-PCR analysis of vWA genes in Magnaporthe oryzae infected rice tissues indicated OsvWA9 , OsvWA36 , OsvWA37 and OsvWA18 as the optimal candidate genes for disease resistance. This is the first attempt to characterize vWA gene family in plant species. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
47. Importance of OsRac1 and RAI1 in signalling of Pigm-1 mediated resistance to rice blast disease
- Author
-
Yang, Dewei, primary, He, Niqing, additional, Huang, Fenghuang, additional, Chen, Jialin, additional, Yu, Minxiang, additional, Jin, Yidan, additional, Lin, Shaojun, additional, and Li, Shengping, additional
- Published
- 2024
- Full Text
- View/download PDF
48. Deep Learning Analysis of Rice Blast Disease Using Remote Sensing Images.
- Author
-
Shubhajyoti Das, Arindam Biswas 0002, Vimalkumar C, and Parimal Sinha
- Published
- 2023
- Full Text
- View/download PDF
49. Novel hybrid ARIMA-BiLSTM model for forecasting of rice blast disease outbreaks for sustainable rice production.
- Author
-
M. Varsha, B. Poornima, M. P. Pavan Kumar, and S. Basavarajappa
- Published
- 2023
- Full Text
- View/download PDF
50. Rice leaf associated Chryseobacterium species: An untapped antagonistic flavobacterium displays volatile mediated suppression of rice blast disease
- Author
-
Kumar, M., Charishma, K., Sahu, K.P., Sheoran, N., Patel, A., Kundu, A., and Kumar, A.
- Published
- 2021
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.