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A continuous protein recovery and purification system based on the true moving bed concept is presented. A novel adsorbent material, in the form of an elastic and robust woven fabric, served as a moving belt following the general designs observed in known belt conveyors. The composite fibrous material that forms the said woven fabric showed high protein binding capacity, reaching a static binding capacity equal to 107.3 mg/g, as determined via isotherm experiments. Moreover, testing the same cation exchange fibrous material in a packed bed format resulted in excellent dynamic binding capacity values (54.5 mg/g) even when operating at high flow rates (480 cm/h). In a subsequent step, a benchtop prototype was designed, constructed, and tested. Results indicated that the moving belt system could recover a model protein (hen egg white lysozyme) with a productivity up to 0.5 mg/cm2/h. Likewise, a monoclonal antibody was directly recovered from unclarified CHO_K1 cell line culture with high purity, as judged by SDS-PAGE, high purification factor (5.8), and in a single step, confirming the suitability and selectivity of the purification procedure.

In this work, a novel technique for continuous purification of biologics from a crude feedstock is demonstrated with equipment referred to as Fluidized Bed Adsorption System (FBRAS). The development and validation of such unit operations were performed utilizing lysozyme as a model protein and Relisorb™ SP405/EB as a carrier. The performance of FBRAS to carry out combined clarification and purification was evaluated by capturing of antifungal peptides directly from the lysed broth. The novel technique reduced the number of process unit operations from six to three without having an impact on purity. Overall productivity increased by 250% in comparison to the existing downstream processing routine., (© 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.)

A continuous protein recovery and purification system based on the true moving bed concept is presented. A novel adsorbent material, in the form of an elastic and robust woven fabric, served as a moving belt following the general designs observed in known belt conveyors. The composite fibrous material that forms the said woven fabric showed high protein binding capacity, reaching a static binding capacity equal to 107.3 mg/g, as determined via isotherm experiments. Moreover, testing the same cation exchange fibrous material in a packed bed format resulted in excellent dynamic binding capacity values (54.5 mg/g) even when operating at high flow rates (480 cm/h). In a subsequent step, a benchtop prototype was designed, constructed, and tested. Results indicated that the moving belt system could recover a model protein (hen egg white lysozyme) with a productivity up to 0.5 mg/cm 2 /h. Likewise, a monoclonal antibody was directly recovered from unclarified CHO_K1 cell line culture with high purity, as judged by SDS-PAGE, high purification factor (5.8), and in a single step, confirming the suitability and selectivity of the purification procedure., Competing Interests: Author CZ was employed by MDX Biotechnik International GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Guo, Kangwa, Ali, Mayer-Gall, Gutmann, Zenneck, Winter, Jungbauer and Fernandez Lahore.)

A fluidized‐bed‐riser adsorption system for continuous bioproduct recovery from crude feedstock. Herlevi, Lisa‐Marie; Fernandez‐Lahore, Marcelo; Ferreira, Guilherme. 2023; 120:10. https://doi.org/10.1002/bit.28458 Performance of a New Family of Modular, Bed‐Supported, Chromatography Devices. Platteau, Gerald; Stroehlein, Guido; Alstine, James Van; Nagaya, Masayoshi. 2023; 120:10. https://doi.org/10.1002/bit.28463 NIR Spectroscopy ‐ CNN‐enabled Chemometrics for Multi‐analyte Monitoring in Microbial Fermentation. Banerjee, Shantanu; Mandal, Shyamapada; Jesubalan, Naveen G.; Jain, Rijul; Rathore, Anurag. 2024; 121:6. https://doi.org/10.1002/bit.28681 All articles for this special issue can be viewed online at https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/toc/10.1002/(ISSN)1097-0290.Recovery-of-Biological-Products-Past-Present-Future [ABSTRACT FROM AUTHOR]