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Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level. Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 mM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plantpathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out. [ABSTRACT FROM AUTHOR]

Existing research on the reception of Fyodor Dostoevsky's works in China primarily focuses on the academic reception history, with limited attention paid to ordinary readers on the internet. This study employs the qualitative analysis software NVivo 12 to analyze comments from ordinary readers on Douban. By integrating Stanley Fish's reader-response criticism and Stuart Hall's reception theory, the study identifies three distinct reader reception models and analyzes their influencing factors. Faithful fans, influenced by the Russian classical literature horizon of expectations, offer highly positive evaluations. Rational evaluators exhibit a complex attitude of both criticism and appreciation, driven by their rational stance and cultural aesthetic differences. Critics, encounter frustration in their expectations due to conflicts between their local aesthetic perspectives and the works. This study broadens the scope of audience in the reception research of Dostoevsky's works in China, revealing the diversity and complexity of ordinary readers' reception, and provides important insights for cross-cultural reader studies. [ABSTRACT FROM AUTHOR]

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