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Linbo Tang,1 Xinjing Li,2 Yongbin Wang,1 Yuanhe Tong1 1Department of Radiation Oncology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan, Fujian, 364000, People’s Republic of China; 2Department of Pathology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan, Fujian, 364000, People’s Republic of ChinaCorrespondence: Yuanhe Tong, Department of Radiation Oncology, Longyan First Affiliated Hospital of Fujian Medical University, Longyan, 364000, People’s Republic of China, Email tyhlongyan@126.comPurpose: Inflammatory markers in the blood have been linked to tumor prognosis, but their specific prognostic significance in nasopharyngeal carcinoma (NPC) patients undergoing intensity-modulated radiotherapy (IMRT) is not well established. This study aims to evaluate the prognostic value of the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) in this patient population.Patients and Methods: A total of 406 non-metastatic NPC patients were included in the study. NLR, PLR, and LMR were stratified according to their average values. The Kaplan-Meier method was used to estimate progression-free survival (PFS) and overall survival (OS). Cox multivariate regression analysis was performed to evaluate the associations of NLR, PLR, and LMR with PFS and OS.Results: Patients with NLR > 2.78 had worse PFS (P = 0.008) and OS (P < 0.001); PLR > 162.48 was related to lower PFS (P = 0.018) but not OS (P = 0.29); LMR > 5.05 showed no significant difference in PFS and OS compared to LMR ≤ 5.05 (P values were 0.13 and 0.94, respectively). Multivariate analysis indicated that NLR was an independent prognostic factor for PFS (HR, 1.674; 95% CI, 1.006– 2.784; P = 0.047) and OS (HR, 4.143; 95% CI, 2.111– 8.129; P = 0.000), while PLR and LMR did not demonstrate significant associations with PFS and OS.Conclusion: This study identifies NLR as a novel and independent prognostic indicator for NPC patients receiving IMRT, offering valuable insights that could inform future clinical decision-making. In contrast, PLR and LMR did not demonstrate significant prognostic value in this context.Keywords: nasopharyngeal carcinoma, inflammatory markers, neutrophil to lymphocyte ratio, platelet to lymphocyte ratio, lymphocyte to monocyte ratio, prognosis

As a feature of global warming, climate change has been a severe issue in the 21st century. A more comprehensive reconstruction is necessary in the climate assessment process, considering the heterogeneity of climate change scenarios across various meteorological elements and seasons. To better comprehend the change in minimum temperature in winter in the Jinsha River Basin (China), we built a standard tree-ring chronology from Picea likiangensis var. balfouri and reconstructed the regional mean minimum temperature of the winter half-years from 1606 to 2016. This reconstruction provides a comprehensive overview of the changes in winter temperature over multiple centuries. During the last 411 years, the regional climate has undergone seven warm periods and six cold periods. The reconstructed temperature sensitively captures the climate warming that emerged at the end of the 20th century. Surprisingly, during 1650–1750, the lowest winter temperature within the research area was about 0.44 °C higher than that in the 20th century, which differs significantly from the concept of the "cooler" Little Ice Age during this period. This result is validated by the temperature results reconstructed from other tree-ring data from nearby areas, confirming the credibility of the reconstruction. The Ensemble Empirical Mode Decomposition method (EEMD) was adopted to decompose the reconstructed sequence into oscillations of different frequency domains. The decomposition results indicate that the temperature variations in this region exhibit significant periodic changes with quasi-3a, quasi-7a, 15.5-16.8a, 29.4-32.9a, and quasi-82a cycles. Factors like El Niño–Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO), and solar activity, along with Atlantic Multidecadal Oscillation (AMO), may be important driving forces. To reconstruct this climate, this study integrates the results of three machine learning algorithms and traditional linear regression methods. This novel reconstruction method can provide valuable insights for related research endeavors. Furthermore, other global climate change scenarios can be explored through additional proxy reconstructions. [ABSTRACT FROM AUTHOR]

Colon cancer has a high mortality rate, thus there is an urgent need to develop novel therapeutic options for clinical management of the disease. Studies have revealed that chaperonin containing TCP1 subunit 6A (CCT6A) promoted the development of multiple types of cancer, and dataset analysis revealed that homeobox B2 (HOXB2) has the potential to modulate the expression of CCT6A. However, whether HOXB2 affects the proliferation, migration and invasion of colon cancer cells remains to be determined. A CCT6A knockdown colon cancer cell line was established and colony formation, wound healing and Transwell invasion assays were performed to assess proliferation, migration and invasion of the altered colon cancer cells. Subsequently, luciferase reporter gene assays and chromatin immunoprecipitation assays were performed to detect the relationship between HOXB2 and CCT6A. A HOXB2 overexpression colon cancer cell line was established and the proliferation, migration and invasion of these cells was determined using the same methods. Knockdown of CCT6A reduced the proliferation, migration and invasion of colon cancer cells. HOXB2 enhanced the expression of CCT6A in colon cancer cells by binding to the promoter of CCT6A. Overexpression of HOXB2 abolished the inhibitory effect of CCT6A knockdown on the proliferation, migration and invasion of colon cancer cells. HOXB2 increased the proliferation and invasiveness of colon cancer cells by increasing the expression of CCT6A. [ABSTRACT FROM AUTHOR]