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In mammals, complex dental microwear textures (DMT) representing differently sized and shaped enamel lesions overlaying each other have traditionally been associated with the seeds and kernels in frugivorous diets, as well as with sclerotized insect cuticles. Recently, this notion has been challenged by field observations as well as in vitro experimental data. It remains unclear to what extent each food item contributes to the complexity level and is reflected by the surface texture of the respective tooth position along the molar tooth row. To clarify the potential of seeds and other abrasive dietary items to cause complex microwear textures, we conducted a controlled feeding experiment with rats. Six individual rats each received either a vegetable mix, a fruit mix, a seed mix, whole crickets, whole black soldier fly larvae, or whole day-old-chicks. These diets were subjected to material testing to obtain mechanical properties, such as Young’s modulus, yield strength, and food hardness (as indicated by texture profile analysis [TPA] tests). Seeds and crickets caused the highest surface complexity. The fruit mix, seed mix, and crickets caused the deepest wear features. Moreover, several diets resulted in an increasing wear gradient from the first to the second molar, suggesting that increasing bite force along the tooth row affects dental wear in rats on these diets. Mechanical properties of the diets showed different correlations with DMT obtained for the first and second molars. The first molar wear was mostly correlated with maximum TPA hardness, while the second molar wear was strongly correlated with maximum yield stress, mean TPA hardness, and maximum TPA hardness. This indicates a complex relationship between chewing mechanics, food mechanical properties, and observed DMT. Our results show that, in rats, seeds are the main cause of complex microwear textures but that hard insect body parts can also cause high complexity. However, the similarity in parameter

Dental microwear texture analysis (DMTA) is widely used for diet inferences in extant and extinct vertebrates. Often, a reference tooth position is analysed in extant specimens, while isolated teeth are lumped together in fossil datasets. It is therefore important to test whether dental microwear texture (DMT) is tooth position specific and, if so, what causes the differences in wear. Here, we present results from controlled feeding experiments with 72 guinea pigs, which received either fresh or dried natural plant diets of different phytolith content (lucerne, grass, bamboo) or pelleted diets with and without mineral abrasives (frequently encountered by herbivorous mammals in natural habitats). We tested for gradients in dental microwear texture along the upper cheek tooth row. Regardless of abrasive content, guinea pigs on pelleted diets displayed an increase in surface roughness along the tooth row, indicating that posterior tooth positions experience more wear compared with anterior teeth. Guinea pigs feedings on plants of low phytolith content and low abrasiveness (fresh and dry lucerne, fresh grass) showed almost no DMT differences between tooth positions, while individuals feeding on more abrasive plants (dry grass, fresh and dry bamboo) showed a gradient of decreasing surface roughness along the tooth row. We suggest that plant feeding involves continuous intake and comminution by grinding, resulting in posterior tooth positions mainly processing food already partly comminuted and moistened. Pelleted diets require crushing, which exerts higher loads, especially on posterior tooth positions, where bite forces are highest. These differences in chewing behaviour result in opposing wear gradients for plant versus pelleted diets.

Strontium isotopes in biogenic apatite, especially enamel, are widely employed to determine provenance and track migration in palaeontology and archaeology. Body tissues record the 87Sr/86Sr of bioavailable Sr of ingested food and water. To identify non-local individuals, knowledge of the 87Sr/86Sr of a non-migratory population is required. However, varying factors such as tissue turnover rates, feeding selectivity, Sr content, digestibility of food, and the ingestion of mineral dust can influence body tissue 87Sr/86Sr. To evaluate the Sr contribution of diet and water to mammalian hard tissues 87Sr/86Sr, controlled feeding studies are necessary. Here we present 87Sr/86Sr from controlled feeding experiments with two rodent species (Rattus norvegicus, Cavia porcellus). Due to the continuous and fast incremental growth of rat and guinea pig incisors (∼0.1 – 0.5 mm/day), their enamel is expected to record isotopic dietary changes. For Experiment-1: Diet Switch, animals were switched from their respective supplier food to a pelleted experimental diet containing either insect-, plant-, or meat-meal and a staggered-sampling approach was used to monitor the 87Sr/86Sr changes in rat incisor enamel and bone over the course of the experiment. In Experiment-2: Basic Diets, separated cohorts (n = 6) of rats and guinea pigs were fed one of the three pelleted diets and received tap water for 54 days. While the rat incisors showed a complete tissue turnover, the slower-growing guinea pig incisors partially retained supplier diet-related isotopic compositions. In addition, one group of rats fed plant-meal pellets received Sr-rich mineral water, demonstrating that drinking water can be an important Sr source in addition to diet. Additionally, a leaching experiment showed that only a small fraction of diet-related Sr is bioavailable. Finally, in Experiment-3: Dust Addition, guinea pigs were fed pellets with and without addition of 4% of isotopically distinct dust (loess or kaolin). A

To differentiate the effects of internal and external abrasives on tooth wear, we performed a controlled feeding experiment in rabbits fed diets of varying phytolith content as an internal abrasive and with addition of sand as an external abrasive. 13 rabbits were each fed one of the following four pelleted diets with different abrasive characteristics (no phytoliths: lucerne L; phytoliths: grass G; more phytoliths: grass and rice hulls GR; phytoliths plus external abrasives: grass, rice hulls and sand GRS) for two weeks. At the end the feeding period, three tooth wear proxies were applied to quantify wear on the cheek teeth at macroscopic and microscopic wear scales: CT scans were obtained to quantify tooth height. Mesowear was scored adapted to this species, and 3D dental microwear texture analysis (DMTA) was performed on four antagonistic teeth. Both external and internal abrasives resulted in increased wear in all proxies compared to the phytolith and sand-free diet (L). The wear effect was more prominent on the maxillary than on the mandibular teeth. On the GRS diet, the upper third premolar had the largest decline in relative tooth height compared to others in the same tooth row. The impact of diet abrasiveness on the mesowear signal was only clearly visible for the most abrasive diet, most likely due to the limited sample size. DMTA was especially sensitive to phytolith changes in the diet, and surface roughness generally increased with increasing amounts of abrasive agents (L < G < GR < GRS) as expressed in an increase of most height and volume parameters. The fast pace of dental wear in this species led to some expected correlations between tooth height, mesowear and DMTA parameters, creating a distinct wear pattern for each diet. Animal models with high wear rates may be particularly suitable for investigations on functional interrelationships of different wear proxies.

In mammals, complex dental microwear textures (DMT) representing differently sized and shaped enamel lesions overlaying each other have traditionally been associated with the seeds and kernels in frugivorous diets, as well as with sclerotized insect cuticles. Recently, this notion has been challenged by field observations as well as in vitro experimental data. It remains unclear to what extent each food item contributes to the complexity level and is reflected by the surface texture of the respective tooth position along the molar tooth row. To clarify the potential of seeds and other abrasive dietary items to cause complex microwear textures, we conducted a controlled feeding experiment with rats. Six individual rats each received either a vegetable mix, a fruit mix, a seed mix, whole crickets, whole black soldier fly larvae, or whole day-old-chicks. These diets were subjected to material testing to obtain mechanical properties, such as Young’s modulus, yield strength, and food hardness (as indicated by texture profile analysis [TPA] tests). Seeds and crickets caused the highest surface complexity. The fruit mix, seed mix, and crickets caused the deepest wear features. Moreover, several diets resulted in an increasing wear gradient from the first to the second molar, suggesting that increasing bite force along the tooth row affects dental wear in rats on these diets. Mechanical properties of the diets showed different correlations with DMT obtained for the first and second molars. The first molar wear was mostly correlated with maximum TPA hardness, while the second molar wear was strongly correlated with maximum yield stress, mean TPA hardness, and maximum TPA hardness. This indicates a complex relationship between chewing mechanics, food mechanical properties, and observed DMT. Our results show that, in rats, seeds are the main cause of complex microwear textures but that hard insect body parts can also cause high complexity. However, the similarity in parameter

Recent studies have shown that phytoliths are softer than dental enamel but still act as abrasive agents. Thus, phytolith content should be reflected in dental wear. Because native phytoliths show lower indentation hardness than phytoliths extracted by dry ashing, we propose that the hydration state of plant tissue will also affect dental abrasion. To assess this, we performed a controlled feeding experiment with 36 adult guinea pigs, fed exclusively with three different natural forages: lucerne, timothy grass, and bamboo with distinct phytolith/silica contents (lucerne < grass < bamboo). Each forage was fed in fresh or dried state for 3 weeks. We then performed 3D surface texture analysis (3DST) on the upper fourth premolar. Generally, enamel surface roughness increased with higher forage phytolith/silica content. Additionally, fresh and dry grass feeders displayed differences in wear patterns, with those of fresh grass feeders being similar to fresh and dry lucerne (phytolith-poor) feeders, supporting previous reports that “fresh grass grazers” show less abrasion than unspecialized grazers. Our results demonstrate that not only phytolith content but also properties such as water content can significantly affect plant abrasiveness, even to such an extent that wear patterns characteristic for dietary traits (browser–grazer differences) become indistinguishable.

Dental mesowear is applied as a proxy to determine the general diet of mammalian herbivores based on tooth-cusp shape and occlusal relief. Low, blunt cusps are considered typical of grazers and high, sharp cusps typical of browsers. However, how internal or external abrasives impact mesowear, and the time frame the wear signature takes to develop, still need to be explored. Four different pelleted diets of increasing abrasiveness (lucerne, grass, grass and rice husks, and grass, rice husks and sand) were fed to four groups of a total of 28 adult goats in a controlled feeding experiment over a 6-month period. Tooth morphology was captured by medical CT scans at the beginning and end of the experiment. These scans, as well as the crania obtained post mortem, were scored using the mesowear method. Comparisons between diet groups showed few significant differences after 6 months, irrespective of whether CT scans or the real teeth were scored. Only when assessing the difference in signal between the beginning and the end of the experiment did relevant, significant diet-specific effects emerge. Diets containing lower phytolith content caused a more pronounced change inmesowear towards sharper cusps/higher reliefs, while the feed containing sand did not result in more extreme changes in mesowear when compared with the same feed without sand. Our experiment suggests that the formation of a stable and hence reliable mesowear signal requires more time to develop than 6 months.