Search

PURPOSE: The murine oxygen-induced retinopathy (OIR) model is one of the most widely used animal models of ischemic retinopathy, mimicking hallmark pathophysiology of initial vaso-obliteration (VO) resulting in ischemia that drives neovascularization (NV). In addition to NV and VO, human ischemic retinopathies, including retinopathy of prematurity (ROP), are characterized by increased vascular tortuosity. Vascular tortuosity is an indicator of disease severity, need to treat, and treatment response in ROP. Current literature investigating novel therapeutics in the OIR model often report their effects on NV and VO, and measurements of vascular tortuosity are less commonly performed. No standardized quantification of vascular tortuosity exists to date despite this metrics relevance to human disease. This proof-of-concept study aimed to apply a previously published semi-automated computer-based image analysis approach (iROP-Assist) to develop a new tool to quantify vascular tortuosity in mouse models. DESIGN: Experimental study. SUBJECTS: C57BL/6J mice subjected to the OIR model. METHODS: In a pilot study, vasculature was manually segmented on flat-mount images of OIR and normoxic (NOX) mice retinas and segmentations were analyzed with iROP-Assist to quantify vascular tortuosity metrics. In a large cohort of age-matched (postnatal day 12 [P12], P17, P25) NOX and OIR mice retinas, NV, VO, and vascular tortuosity were quantified and compared. In a third experiment, vascular tortuosity in OIR mice retinas was quantified on P17 following intravitreal injection with anti-VEGF (aflibercept) or Immunoglobulin G isotype control on P12. MAIN OUTCOME MEASURES: Vascular tortuosity. RESULTS: Cumulative tortuosity index was the best metric produced by iROP-Assist for discriminating between OIR mice and NOX controls. Increased vascular tortuosity correlated with disease activity in OIR. Treatment of OIR mice with aflibercept rescued vascular tortuosity. CONCLUSIONS: Vascular tortuosity is a quantifiable feature of the OIR model that correlates with disease severity and may be quickly and accurately quantified using the iROP-Assist algorithm. FINANCIAL DISCLOSURES: Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.

OBJECTIVE: To develop a generative adversarial network (GAN) to segment major blood vessels from retinal flat-mount images from oxygen-induced retinopathy (OIR) and demonstrate the utility of these GAN-generated vessel segmentations in quantifying vascular tortuosity. DESIGN: Development and validation of GAN. SUBJECTS: Three datasets containing 1084, 50, and 20 flat-mount mice retina images with various stains used and ages at sacrifice acquired from previously published manuscripts. METHODS: Four graders manually segmented major blood vessels from flat-mount images of retinas from OIR mice. Pix2Pix, a high-resolution GAN, was trained on 984 pairs of raw flat-mount images and manual vessel segmentations and then tested on 100 and 50 image pairs from a held-out and external test set, respectively. GAN-generated and manual vessel segmentations were then used as an input into a previously published algorithm (iROP-Assist) to generate a vascular cumulative tortuosity index (CTI) for 20 image pairs containing mouse eyes treated with aflibercept versus control. MAIN OUTCOME MEASURES: Mean dice coefficients were used to compare segmentation accuracy between the GAN-generated and manually annotated segmentation maps. For the image pairs treated with aflibercept versus control, mean CTIs were also calculated for both GAN-generated and manual vessel maps. Statistical significance was evaluated using Wilcoxon signed-rank tests (P ≤ 0.05 threshold for significance). RESULTS: The dice coefficient for the GAN-generated versus manual vessel segmentations was 0.75 ± 0.27 and 0.77 ± 0.17 for the held-out test set and external test set, respectively. The mean CTI generated from the GAN-generated and manual vessel segmentations was 1.12 ± 0.07 versus 1.03 ± 0.02 (P = 0.003) and 1.06 ± 0.04 versus 1.01 ± 0.01 (P < 0.001), respectively, for eyes treated with aflibercept versus control, demonstrating that vascular tortuosity was rescued by aflibercept when quantified by GAN-generated and manual vessel segmentations. CONCLUSIONS: GANs can be used to accurately generate vessel map segmentations from flat-mount images. These vessel maps may be used to evaluate novel metrics of vascular tortuosity in OIR, such as CTI, and have the potential to accelerate research in treatments for ischemic retinopathies. FINANCIAL DISCLOSURES: The author(s) have no proprietary or commercial interest in any materials discussed in this article.

Introduction: To ensure higher productivity, fertilizers have been excessively applied to the fruit greenhouse soil yearly, thus resulting in the increasing risks of residual nitrate leaching in the North China Plain. Methods: In this study, a water and solute transport HYDRUS-1D model was used to evaluate the effects of using sweet corn as a catch crop on deep water drainage and nitrate leaching in a sweet cherry greenhouse soil. A three-year (2019–2021) field experiment was conducted during the rainfall season from July to September in the post-harvest of sweet cherry, when the plastic cover was removed each year. In the experiment, the five treatments were designed. The three nitrate residue levels denoted by CKR, N1R, and N2R, represented nitrate residue amounts in the soil profile of three nitrogen fertilizer levels(0, 280 and 420kg ha-1) before the harvest of sweet cherry(March to June). Two other treatments with and without sweet corn as a catch crop based on the treatments of N1R and N2R were denoted by N1RC and N2RC, respectively. The data of both the spatial and temporal distribution of water and nitrate content during the rainy seasons of 2019, 2020 and 2021 in the field experiment were collected to calibrate and validate the model. Results: The simulated results have showed that using sweet corn as a catch crop increased the evapotranspiration rate, the upward flux of water and nitrate at a 100 cm soil depth reached a maximum of 1.5 mm d-1 and 1.0 kg N ha-1d-1, respectively, and the downward movement of water and nitrate leached to deeper soil layers was reduced. Compared with CKR, the treatments with catch crops (N1RC and N2RC) reduced the amount of water drainage by 16.4% -47.7% in the 0-180cm soil profile. The average amounts of nitrate leaching in the 1.8 m soil profile during the three-year experiment were 88.1, 113.3, and 58.2 kg N ha−1 for the treatment without catch crop (N1R and N2R) and 32.3, 54.8, and 31.4 kg N ha−1 for the treatment with catch crop (N1RC and N2RC), respectively. The treatments (N1RC and N2RC) with catch crops decreased the amount of nitrate leaching by 29.6%-69.1% compared with the treatments without catch crops (N1R and N2R). Discussion: Sweet corn as summer catch crop can reduce nitrate leaching in the sweet cherry greenhouses. Our study has provided an effective method to reduce the risk of nitrate leaching for sweet cherry greenhouses in the North China Plain. [ABSTRACT FROM AUTHOR]

Synergistic fermentation of coal and corn straw is an effective tool to increase biomethane production. However, a large gap exists between the biomethane production conditions of corn straw filling coal mine goafs and laboratory experiments. In order to determine the effect of the field environment on synergistic biomethane production, biomethane production experiments with coal and corn straw were carried out under different conditions to find the key factors restricting the potential of biomethane production. The obtained results showed that various bacterial sources had significant influences on the biomethane production of coal and corn straw, and domesticated bacterial sources provided fermentation systems with more efficient biomethane production capacities than mine water sources. Biomethane production of coal and corn straw was relatively high under mixed conditions, but it was also promoted under unmixed conditions. Different inorganic minerals had different effects on synergistic biomethane production, which varied. For example, calcite, montmorillonite, and kaolin are common minerals in coal‐bearing strata that significantly enhance synergistic biomethane production of coal and corn straw. However, pyrite was found to significantly inhibit the synergistic biomethane production effect of coal and corn straw. Highly metamorphosed anthracite coal also presented biomethane production potential when stimulated by corn straw as a carbon source. The obtained results revealed the influences of different field conditions on the biomethane production of coal and corn straw and provided a reference for the field application of corn straw filling in coal mine goafs. [ABSTRACT FROM AUTHOR]

This study addressed the effect of microbial methane production on the physical properties of a coal reservoir. Two kinds of coal samples before and after anaerobic degradation were tested by a low-temperature liquid nitrogen adsorption test and an isothermal adsorption and diffusion coefficient test. The influence of the characteristics of microbial gas production on the coal physical properties was analyzed. Due to the differences in the physical properties of the coal samples, the effect of microbial production is different. Coal is a macromolecular organic compound, mainly aromatic and lignin derivatives, containing carbon and nitrogen sources that can be used by microorganisms. Microorganisms secrete extracellular enzymes to decompose covalent bonds and functional groups of macromolecules in coal and eventually produce methane, which will change the physical properties of coal. It was found that microbial anaerobic degradation could increase the content of coalbed methane, change the pore structure of coal, reduce the fractal dimension of the coal surface and smooth the coal surface. At the same time, microbial degradation has changed the physical properties of coal reservoirs to some extent, increased the diffusion of coal reservoirs and improved the pore connectivity of coal reservoirs, which provides more of a scientific basis for the development of coalbed methane. [ABSTRACT FROM AUTHOR]

Selective Laser Melting (SLM) is an effective technology for fabricating new types of porous metal-bonded diamond tools with complex geometries. However, due to the high cooling rate and internal stresses during SLM fabrication, defects such as high porosities and interface gaps still need to be resolved before it can be considered for use in other applications. The influence of heat treatment temperature on internal characterization, interface microstructures, and tensile properties of AlSi7Mg-bonded diamond composites fabricated by SLM were investigated in this work. From experimental results, the porosities of HT-200, HT-350, and HT-500 specimens were 12.19%, 11.37%, and 11.14%, respectively, showing a slightly lower percentage than that of the No-HT specimen (13.34%). Here, HT represents "Heat Treatment". For No-HT specimens, an obvious un-bonding area can be seen in the interface between AlSi7Mg and diamond, whereas a relative closer interface can be observed for HT-500 specimens. After heat treatment, the elastic modulus of specimens showed a relative stable value (16.77 ± 2.79~18.23 ± 1.72 GPa), while the value of yield strength decreased from 97.24 ± 4.48 to 44.94 ± 7.06 MPa and the value of elongation increased from 1.98 ± 0.05 to 6.62 ± 0.51%. This difference can be attributed mainly to the disappearance of the solid-solution hardening effect due to the increase of Si content after heat treatment. [ABSTRACT FROM AUTHOR]