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Background: Malaria continues to be among the leading causes of mortality in Africa including Uganda, with the emergence of parasite resistance to the first-line therapeutics (Artemisinin- based Combination Therapy). To find new therapeutics, this study has reported an in vivo antimalarial efficacy of combinations of Artemisia annua (Aa), Vernonia amygdalina (Va), and Microglossa pyrifolia (Mp) in mice model using factorial design., Methods: The Aa and Va were extracted by hot infusion, and Mp by cold maceration using distilled water. The dry extracts were screened for different phytochemicals, and later subjected to in vivo antimalarial activity using Peter's 4-day suppressive test. The 2 3 factorial design used Aa, Va, and Mp aqueous extracts as independent variables at two levels (-1 and 1), and the percentage chemo suppression and survival time as response variables. The data was analyzed using Design Expert 13 and GraphPad Prism employing ANOVA linear regression modelling and t-test respectively., Results: All the extracts had alkaloids, phenols, saponins, terpenoids, cardiac glycosides, tannins, steroids, and carbohydrates. The various combinations showed chemo suppression from 41.5 to 91.0% and survival time of 19 to 23 days. The first three combinations having lower levels of Aa (200 mg/kg) exhibited higher chemo suppression (> 90%) compared to Artemisinin-Lumefantrine positive control at 4 mg/kg with 87.5%. Lower levels of Aa in the combinations contributed to high chemo suppression while higher levels of Va prolonged survival times. Interactions between Aa and Mp showed higher chemo suppression, and that between Aa and Va increased survival time. An optimized prediction of 94.4% chemo suppression was made by the ANOVA model at lower levels of Aa and Va, and a higher level of Mp, which is similar to an experimental run which gave a response of 90. 6%., Conclusion: An optimum combination of the three plants as a natural herbal antimalarial therapy was obtained using factorial design, and it offers an alternative to first line Artemisinin based Combination Therapy (ACTs) as parasite resistance looms. This combination could be further developed into a standard phytopharmaceutical and subjected to Randomized Controlled Trials (RCTs)., Competing Interests: Declarations Ethics approval and consent to participate The research and animal experimental protocols used in this study were reviewed and approved at various levels at Mbarara University of Science and Technology (Uganda). The technical approvals were obtained at the department of pharmaceutical sciences and pharmacy, and Faculty Research Committee (FRC). The final research ethical approval/ permission for use of laboratory animals was obtained from Mbarara University of Science and Technology Research Ethical Committee (MUST-REC, MUST-2021–171). All the research assistants and technical staff who participated in conduct of the experiments received appropriate trainings in animal care and use, then on management of pain and distress in laboratory mice and rats. All the approved procedures in the protocol were performed and reported in accordance with Animal Research Reporting of In Vivo Experiments (ARRIVE) guidelines and regulations. Consent for publication The work does not contain any individual or personal data in form of images or videos that requires consent before publication. Competing interests The authors declare no competing interests., (© 2024. The Author(s).)

Introduction: Vernonia amygdalina Delile (VAD), also known as bitter leaf, is widely utilized in traditional medicine for the treatment of various ailments, including cancer. The presence of bioactive compounds in VAD is believed to be responsible for its characteristic bitterness. In Ghana, it is a common practice to mitigate the bitterness of VAD by combining it with Citrus aurantifolia (Christm.) Swingle (lime) juice extracts, although this method lacks scientific evidence and documentation. Therefore, the antioxidant and anticancer activities of VAD and lime juice extracts (V5) and their combined effects were evaluated in vitro. Method: The antioxidant activity and cytotoxic effects of VAD extracts were determined against Jurkat, MCF-7, HepG2, and PNT2 cells using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to quantify antioxidant activity and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess cytotoxicity. The statistical analysis of the data was conducted using Microsoft Excel and GraphPad Prism 8.0. Linear regression was employed to determine the correlation between the concentration and the percentage of antioxidant activity, while p values were calculated using Student's t -test. Results: The laboratory analysis focused on the extracts V1, V2, V3, V4, and V5. Briefly, V1 and V2 contained equal amounts of saponins and terpenoids. Among these, V2 exhibited the highest free radical scavenging activity, as indicated by an EC50 value of 2.14 ± 0.06 mg/mL. V2 also demonstrated cytotoxicity against the MCF-7, HepG2, Jurkat, and PNT2 cell lines. On the other hand, V3 and V4 did not show any cytotoxic effects across all tested cell lines. In contrast, V5 was toxic to HepG2 and MCF-7 cells but had no cytotoxic effect on Jurkat cell lines. V2 exhibited dose-dependent cytotoxicity (0-1000  μ g/mL), with the strongest inhibition observed against Jurkat cells (IC50 value = 96.341  μ g/mL) and a selective index of 3.567. The difference in activity between the extracts from different parts of the plant and the extract combined with lime juice was significant ( p < 0.05), indicating a synergistic effect of the phytochemicals in both VAD and lime juice. Conclusion: V2 and V5 demonstrated a remarkable antioxidant property, and they are effective in inhibiting cancer cell lines, respectively., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2024 Eunice E. Ampem Danso et al.)

Objective: This study is targeted at assessing the chemotherapy factor of the ethanol extract of Vernonia amygdalina Delile (VAD)., Methods: U87 glioblastoma cells were treated with extract and a fraction of Vernonia amygdalina Delile (VAD) was harvested from the herbarium. Cytotoxicity was evaluated to determine the IC50 through microscopic observation followed by an MTT assay. Subsequently, flow cytometry with a FACS type was employed to conduct cell cycle and apoptosis analyses. Annexin V/PI and PI markers were used to assess apoptosis and cell cycle progression., Result: The ethanol extract and ethyl acetate fraction of VAD showed promising effects as cancer chemotherapy in glioblastoma cells. The IC50 values for the extract and fraction were notably low, at 37.65 µg/ml and 10.12 µg/ml, respectively, for U87 cells. Analysis of apoptosis using FACS revealed a more pronounced apoptotic effect of the 15 µg/ml fraction of VAD on both early and late apoptosis compared to the 75 µg/ml extract of VAD. Although some differences in cell cycle properties were observed, there were no significant differences in cell cycle analysis between the extract and fraction., Conclusion: These findings underscore the efficacy of VAD's ethanol extract and ethyl acetate fraction as chemotherapeutic agents against U87 cancer cells. The low IC50 values and significant induction of early apoptosis highlight the cytotoxic effects of these treatments on U87 cells.

This study aimed to investigate the impact of Vernonia amygdalina leaf extract on FLT3 regulation. V. amygdalina was extracted with 96% ethanol (VAE-96), and its cytotoxicity against FLT3- cell lines (MOLM-13 and MV-4-11) was evaluated. The results indicated that VAE-96 induced apoptosis in these cells and inhibited the phosphorylation of AKT, MAPK, and FLT3. Additionally, VAE-96 substantially diminished the activity of the FLT3 promoter and the expression of FLT3 mRNA. The extract was found to contain alkaloids, saponin, reduced sugar compounds, and polyphenols, including tannins and flavonoids, as per the phytoconstituents analysis. The potential of alkaloid fractions on MOLM-13 cells was indicated by the robust cytotoxic effect of the alkaloid fractions, which resulted in over 50% cell mortality at 30 µg/ml. Our results suggest that VAE-96 may be a beneficial agent for the prevention and treatment of AML with FLT3-ITD mutation.

The fingerprint of Vernonia anthelmintica effective part (VAEP) from 15 different producing areas was established, followed by cluster analysis and principal component analysis. The relationship between the fingerprint and the melanogenesis-promoting activity of VAEP was then analyzed using the grey correlation degree and the orthogonal partial least square method. The characteristic peaks reflecting the pharmacodynamic effect of VAEP were identified as vernodalin, 3,5-O-dicaffeoyl quinic acid (3,5-diCQA), and butin. Based on the distribution characteristics of these components in plants from different habitats and the verification of results from the spectrum-effect relationship, vernodalin and 3,5-diCQA can be used as characteristic components for quality control and pharmacodynamic assessment of V. anthelmintica products. This research establishes a theoretical foundation for planting areas and provides a scientific evaluation of the melanogenesis-promoting effect of V. anthelmintica., (© 2024 John Wiley & Sons Ltd.)

Chemical investigations of a methanolic extract of the twigs of Vernonia amygdalina Delile (Asteraceae) resulted in the isolation and identification of three previously undescribed highly oxygenated Δ 7,9(11) stigmastane-type steroids namely vernonins U-W (1-3) along with six known compounds (4-9). The structural characterization of all the isolated compounds has been conducted via comprehensive 1D and 2D-NMR spectroscopy as well as HRMS. The seven steroidal derivatives 1-7 were evaluated for their antiplasmodial activity against the chloroquine-resistant strain P. falciparum Dd2 (PfDd2) and their hemolytic effect on human red blood cells (RBCs). Vernonins U (1), A (4) and stigmasterol-3-O-β-d-glucopyranoside (7) showed the highest activity with IC 50 values of (5.47 ± 0.01) μg/mL, (6.02 ± 0.13) μg/mL and (6.34 ± 0.80) μg/mL, respectively, against PfDd2, while vernonin W (3) showed moderate activity of (21.20 ± 0.40) μg/mL. None of the tested compounds displayed hemolytic effects on human RBCs up to 100 μg/mL indicating their safety. These results enrich the known chemistry of V. amygdalina and support its use in folk medicine for the treatment of malaria. This encourages further research towards new antiplasmodial drug candidates from this plant., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Ethnopharmacological Relevance: Pastoralists in Nigeria mix Garcinia kola seed (GK), Khaya senegalensis stem bark (KS), and Vernonia amygdalina leaves (VA) to treat leptospirosis., Aim: To determine the in vitro and in vivo effect on single and combination therapy on Leptospira interrogans-infected mice., Materials and Methods: Evaluation of in vitro assay for anti-leptospiral motility of the extracts was carried out in triplicates. For the in vivo assessment, 40 adult male mice inoculated with Leptospira were randomly allocated into 8 groups of 5 mice each. Groups IV-IX were treated with 800 mg/kg b.w. of KS, GK, VA, KS + GK, KS + VA, GK + VA for 5 days. Group I was negative control, II was model control, and III was treated with penicillin (3.7 mg/kg b.w.) intramuscularly., Results: In vitro, at 90 min, all the extracts at 800, 400, and 200 mg/ml showed complete cessation of motility which was significantly (p < 0.05) different when compared to the negative control. A significant (p < 0.05) IC 50 of 0.18 mg/ml was recorded with GK when compared to KS (0.40 mg/ml), VA (0.25 mg/ml), and procaine penicillin (0.31 mg/ml). Mean packed cell volume, haemoglobin concentration, and mean corpuscular haemoglobin concentration decreased significantly (p < 0.05) in all infected groups and returned to almost pre-infection values. However, significant leucocytosis (p < 0.05) was observed in group II. AST and ALP showed a significant increase (p < 0.001). Histopathological evaluation showed the extracts to prevent the distortion of normal architecture of the selected organs., Conclusion: This study demonstrates the significant potential of Garcinia kola, Khaya senegalensis, and Vernonia amygdalina extracts singly and in combination to combat leptospirosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Nine new germacranolides, sylvaticalides A-H (1-9), and three known analogues (10-12) were isolated from the aerial part of Vernonia sylvatica. Their structures were established using comprehensive spectroscopic analysis, including high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS) and 1D and 2D nuclear magnetic resonance (NMR) spectra. Their absolute configurations were determined by X-ray diffraction experiments. The anti-inflammatory activities of all isolated compounds were assessed by evaluating their inhibitory effects on the nuclear factor kappa B (NF-κB) pathway, which was activated by lipopolysaccharide (LPS)-stimulated human THP1-Dual cells, and the interferon-stimulated gene (ISG) pathway, activated by STING agonist MSA-2 in the same cell model. Compounds 1, 2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways, with IC 50 values ranging from 4.12 to 10.57 μmol·L -1 ., (Copyright © 2024 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.)

Abstract Background Malaria continues to be among the leading causes of mortality in Africa including Uganda, with the emergence of parasite resistance to the first-line therapeutics (Artemisinin- based Combination Therapy). To find new therapeutics, this study has reported an in vivo antimalarial efficacy of combinations of Artemisia annua (Aa), Vernonia amygdalina (Va), and Microglossa pyrifolia (Mp) in mice model using factorial design. Methods The Aa and Va were extracted by hot infusion, and Mp by cold maceration using distilled water. The dry extracts were screened for different phytochemicals, and later subjected to in vivo antimalarial activity using Peter’s 4-day suppressive test. The 23 factorial design used Aa, Va, and Mp aqueous extracts as independent variables at two levels (-1 and 1), and the percentage chemo suppression and survival time as response variables. The data was analyzed using Design Expert 13 and GraphPad Prism employing ANOVA linear regression modelling and t-test respectively. Results All the extracts had alkaloids, phenols, saponins, terpenoids, cardiac glycosides, tannins, steroids, and carbohydrates. The various combinations showed chemo suppression from 41.5 to 91.0% and survival time of 19 to 23 days. The first three combinations having lower levels of Aa (200 mg/kg) exhibited higher chemo suppression (> 90%) compared to Artemisinin-Lumefantrine positive control at 4 mg/kg with 87.5%. Lower levels of Aa in the combinations contributed to high chemo suppression while higher levels of Va prolonged survival times. Interactions between Aa and Mp showed higher chemo suppression, and that between Aa and Va increased survival time. An optimized prediction of 94.4% chemo suppression was made by the ANOVA model at lower levels of Aa and Va, and a higher level of Mp, which is similar to an experimental run which gave a response of 90. 6%. Conclusion An optimum combination of the three plants as a natural herbal antimalarial therapy was obtained using factorial design, and it offers an alternative to first line Artemisinin based Combination Therapy (ACTs) as parasite resistance looms. This combination could be further developed into a standard phytopharmaceutical and subjected to Randomized Controlled Trials (RCTs).

Vernonia cinerea (L.) Less. is a perennial herbaceous plant found mainly in tropical areas, particularly in Southeast Asia, South America, and India. Various parts of V. cinerea have traditionally been used in folk medicine to treat several diseases, such as malaria, fever, and liver diseases. V. cinerea has so far yielded about 92 secondary metabolites. The majority of these are sesquiterpene lactones, but triterpenes, flavonoids, steroids, phenolics, and other compounds are present as well. V. cinerea crude extracts reportedly exhibit anti-inflammatory, antiprotozoal, antidiabetic, anticancer, antimicrobial, antioxidant, and renoprotective activities. This study aims to provide the latest up-to-date information on the botanical characterization, distribution, traditional uses, phytochemistry, and pharmacological activity of V. cinerea . Information on V. cinerea was thoroughly reviewed. The literature published between 1950 and 2024 was compiled through online bibliographic databases, including SciFinder, Web of Science, Google Scholar, PubMed, ScienceDirect, Springer Link, Wiley, and the MDPI online library. The keywords used for the literature search included Vernonia cinerea (L.) Less. and the synonyms Cyanthillium cinereum (L.) H.Rob., Conyza cinerea L., and various others.

Ethnopharmacological Relevance: Sri Lankan traditional medicine uses Vernonia zeylanica and Mallotus repandus broadly for the treatment of a multitude of disease conditions, including wound healing., Aim of the Study: We aimed to scientifically validate the safety and efficacy of wound healing of an aqueous distillate of Vernonia zeylanica and Mallotus repandus (ADVM) mature leaves, tested on primary human dermal fibroblasts., Materials and Methods: Human dermal fibroblasts isolated from clinical waste from circumcision surgery were characterized by flowcytometry and trilineage differentiation. The MTT dye reduction assay, and the ex vivo wound healing scratch assay established wound healing properties of ADVM using the primary human dermal fibroblast cell line. Upregulation of genes associated with wound healing (MMP3, COL3A1, TGFB1, FGF2) were confirmed by RT qPCR. GC-MS chromatography evaluated the phytochemical composition of ADVM., Results: Compared to the synthetic stimulant, β fibroblast growth factor, ADVM at 0.25% concentration on the primary dermal fibroblast cell line exhibited significant ex vivo, (i) 1.7-fold % cell viability (178.7% vs 304.3 %, p < 0.001), (ii) twofold greater % wound closure (%WC) potential (47.74% vs 80.11%, p < 0.001), and (iii) higher rate of % WC (3.251 vs 3.456 % WC/h, p < 0.05), sans cyto-genotoxicity. Up regulated expression of FGF2, TGFB1, COL3A1 and MMP3, genes associated with wound healing, confirmed effective stimulation of pathways of the three overlapping phases of wound healing (P < 0.05). GC-MS profile of ADVM characterized four methyl esters, which may be posited as wound healing phytochemicals., Conclusions: Exceeding traditional medicine claims, the exvivo demonstration of rapid skin regeneration, reiterated by upregulated expression of genes related to wound healing pathways, sans cytotoxicity, propounds ADVM, cued from traditional medicine, as a potential safe and effective natural stimulant for rapid wound-healing. Additionally, it may serve as an effective proliferative stimulant of dermal fibroblasts for cell therapy, with potential in reparative and regenerative therapy of skin disorders., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Five unusual alkaloids featuring a pyrrolo[1,2-a]quinolone skeleton (pyrroloquinolones B-F, 1-5) were isolated from the ethanol extract of the whole plant of Vernonia glabra (Steetz) Vatke, along with sixteen known compounds. Their structures were established by means of spectroscopic (1D and 2D NMR, UV, IR, and ECD) and high resolution mass spectrometric techniques as well as by comparison of their spectroscopic data with those reported in the literature. The ethanol extract and some isolated compounds were assessed for their antibacterial activity against four bacterial strains. The extract was significantly active against Staphylococcus aureus ATCC1026 and S. epidermidis ATCC35984 (MIC = 64 μg/mL). All the tested compounds showed moderate activity against S. epidermidis (16 ≤ MIC ≤ 64 μg/mL). Furthermore, this is the first report on tricyclic pyrrolo[1,2-a]quinolone alkaloids from a plant source. A biosynthetic pathway for the formation of these compounds is also proposed., Competing Interests: Declaration of competing interest The authors declare that they have no competing interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Vernonia patula Merr. (VP) is a traditional medicine used by the Zhuang and Yao people, known for its therapeutic properties in treating anemopyretic cold and other diseases. Distinguishing VP from similar varieties such as Praxelis clematidea (PC), Ageratum conyzoides L. (AC) and Ageratum houstonianum Mill (AH) was challenging due to their similar traits and plant morphology. The HPLC fingerprints of 40 batches of VP and three similar varieties were established. SPSS 20.0 and SIMCA-P 13.0 were used to statistically analyze the chromatographic peak areas of 37 components. The results showed that the similarity of the HPLC fingerprints for each of the four varieties was >0.9, while the similarity between the control chromatogram of VP and its similar varieties was <0.678. Cluster analysis and partial least squares discriminant analysis provided consistent results, indicating that all four varieties could be individually clustered together. Through further analysis, we found isochlorogenic acid A and isochlorogenic acid C were present only in the original VP, while preconene II was present in the three similar varieties of VP. These three components are expected to be identification points for accurately distinguishing VP from PC, AC and AH.

The aim of this study was to screen the phytochemicals and assess the antibacterial properties of crude extracts from Vernonia amygdalina leaves against specific pathogenic bacteria. Qualitative and qauntitave analysis was performed on both ethanol and ethyl acetate crude extracts using standardized techniques. The analysis revealed the presence of alkaloids, phenol, flavonoids, terpenoids, saponins, tannins, and cardiac glycosides in the ethanol extract, while saponins and glycosides were absent in the ethyl acetate extract. The quantities of phenols, flavonoids, and alkaloids were determined using Gallic acid, quercetin, and atropine assays, respectively. The ethanol extract contained 154.7 ± 3.6 (mg GAE/g) of total phenols, 33.3 ± 1.8 (mg QE/g) of flavonoids, and 2.4 ± 0.08 (mg AE) of alkaloids, whereas the ethyl acetate extract contained 93.7 ± 3.9 (mg GAE/g) of total phenols, 30.7 ± 8.3 (mg QE/g) of flavonoids, and 1.57 ± 0.3 (mg GAE/g) of alkaloids. This study identified 44 components in the ethanol crude extract and 10 components in the ethyl acetate crude extract. The presence of alkanes, alkenes, amines, carboxylic acids, and alcohols was confirmed by FT-IR analysis. Each crude extract was tested at concentrations of 500 mg/mL, 250 mg/mL, 125 mg/mL, and 62.5 mg/mL using the same method. A concentration of 0.001 mg/mL of Penicillin capsule standard drugs and dimethyl sulfoxide were used as positive and negative controls, respectively. Overall, the results of the study indicated that both crude extracts exhibited antibacterial activity at higher concentrations.

Vernonia volkameriifolia DC. 1836, is a woody shrub of the subfamily Vernonioideae within the family Asteraceae. The complete chloroplast genome of V. volkameriifolia spans 152,934 bp, comprising four subregions: a large single-copy region (84,035 bp), a small single-copy region (18,543 bp), and a pair of inverted repeats (25,178 bp). Within the chloroplast genome of V. volkameriifolia, 114 unique genes were identified, including 80 unique protein-encoding genes, four ribosomal RNA (rRNA) genes, and 30 transfer RNA (tRNA) genes. Phylogenetic analysis based on the complete chloroplast genome of six related taxa in the Vernonieae tribe indicates that V. volkameriifolia show closer relationships with Vernonia parishii and Strobocalyx arborea. The first chloroplast genome of V. volkameriifolia was reported in this work, contributing to the enrichment of genomic data for the genus Vernonia.

Abstract Background Numerous plants have been explored for their potential antidiabetic properties, and Vernonia amygdalina (VA) stands among them. This study aims to investigate the antidiabetic activities of VA and validate its efficacy. Methods An aqueous extract of Vernonia amygdalina leaves was obtained through maceration. The antidiabetic effects of this plant extract were evaluated in vivo using diabetic model rats. Albino Wistar rats were induced into a diabetic state through intraperitoneal injection of streptozocin and subsequently treated with an optimal dose of 250 mg/kg aqueous extract of VA over a 21-day period. Parameters such as body weight, blood glucose levels, and serum marker enzymes were measured. Results The results demonstrated a significant reduction (p

Exopolysaccharide (EPS) is the main active metabolite of Ganoderma lingzhi in liquid fermentation. In order to increase the content of Ganoderma lingzhi EPS, this study added Vernonia amygdala leaf powder to the fermentation medium, and used single factor experiments and orthogonal experiments to optimize fermentation conditions. Fourier transform infrared spectroscopy was used to characterize the structure of Ganoderma lingzhi EPS, then its antioxidant activity was detected. The single factor experiment found that when the optimal addition amount of Vernonia amygdala leaf powder was 4g/L, the content of EPS increased by 167% compared with the control group. The optimum fermentation conditions of the orthogonal experiment were as follows: Fermentation duration was 12 d, initial pH was 5.0, the rotation speed was 120 r/min, and the addition amount of Vernonia amygdala leaf powder was 4 g/L. Under such a condition, the content of EPS could reach 13.05 g/L. The infrared spectra showed that the major absorption peaks of the Ganoderma lingzhi EPS from the addition of Vernonia amygdala leaf powder were similar to those of the EPS from the control group. The antioxidant activity assay showed that the addition of Vernonia amygdala leaf powder had less effect on the scavenging of ABTS+ radicals by EPS, but enhanced their ability to scavenge DPPH radicals, OH radicals, and ferric ion reduction. The results showed that Vernonia amygdala leaf could effectively increase the content of EPS in Ganoderma lingzhi, which provided a new idea for the efficient production of polysaccharides in Ganoderma lingzhi.

Vernonia cinerea L., also known as purple fleabane, is a plant with medicinal properties that have been traditionally used to treat respiratory infections, digestive disorders, and skin conditions. Its antimicrobial and antiinflammatory properties make it a potential candidate for treating various infections and inflammatory diseases. The plant contains alkaloids, flavonoids, and essential oils, which possess antimicrobial properties, making it a promising candidate for treating bacterial and fungal infections. Its anti-inflammatory properties have shown potential for managing inflammatory diseases like arthritis and dermatitis. Additionally, the plant's analgesic and antipyretic effects suggest its potential for pain management and fever reduction. Its antioxidant properties make it a potential candidate for preventing and treating oxidative stress-related diseases like cardiovascular disorders and neurodegenerative conditions. However, more studies are needed to determine its optimal dosage, safety profile, and potential drug interactions before widespread use in medical practice. [ABSTRACT FROM AUTHOR]

Introduction: Numerous plants in the Vernonia genus are used in Traditional Chinese Medicine (TCM) to treat a wide range of illnesses, such as pityriasis rubra pilaris, infections, wounds, dermatitis, mastitis, diarrhoea, and colds. These genera also have antibacterial, antipyretic, and anti-inflammatory properties. The current work sought to investigate the bactericidal and phytochemical characteristics of Vernonia squarrosa leaf extract and comprehend antibacterial action using molecular docking. Methods: An in vitro study was carried out to investigate the antibacterial capabilities of V. squarrosa. HRLCMS analysis was conducted to determine the phytoconstituents of the chloroform extract (LC). For molecular docking validation, PyRx, an Open Babel integrated Auto Dock programme, was used. Results: Present investigation reveals strong antibacterial activity against two tested gram-positive and gram-negative bacterial strains compared to other solvent extracts, LC at a higher concentration demonstrated a better response. Against Bacillus subtilis (20.05±0.88 mm) and Escherichia coli (20 ± 0.00 mm), the active components of the extracts formed the largest inhibitory zone followed by Pseudomonas aeruginosa (19.50±0.50 mm) and Staphylococcus aureus (14.33 ± 0.33 mm). The LC extract had lower minimum inhibitory concentrations (MIC) and stronger bactericidal activity than the other extracts. Discussion: V. squarrosa's LC showed higher levels of antibacterial activity against the tested bacterial strains than other solvent extracts, most likely as a result of the presence of 31 chemical components, including flavonoids, glycosides, alkaloids, phenolics, and triterpenoids. Developing unique TCM formulations assume significance in global healthcare scenarios to combat bacterial infections and in this context, the current paper underlines the importance of the potent antimicrobial properties of chloroform extract of V. squarrosa. This study revealed that chloroform extract of V. squarrosa has a great deal of antibacterial activity. Conclusion: According to the study, V. squarrosa leaf chloroform extract possesses antibacterial properties because of its active phytocompounds. Its wide range of bioactive components makes it a prime choice for developing novel formulations to neutralize threats of newly emerging strains of harmful bacteria. The aforementioned discoveries may deepen our comprehension of phenomenal wisdom and deep science associated with the legendary TCM.

Two new vernonioside K ( 1 ) and vernonioside L ( 2 ) and four known Δ 7,9(11) stigmastane-type steroidal saponins-vernonioside B2 ( 3 ), vernoniacum B ( 4 ), vernonioside B1 ( 5 ), and vernoamyoside A ( 6 )-were isolated from the leaves of Vernonia amygdalina . Their structures were determined by comprehensive spectroscopic analysis with one-dimensional nuclear magnetic resonance, two-dimensional nuclear magnetic resonance, and high-resolution mass spectrometry. All isolated compounds ( 1 - 6 ) were evaluated to determine their inhibitory effects on α -glucosidase and xanthine oxidase. Among them, two new compounds 1 and 2 showed significant inhibition of α -glucosidase with IC 50 values of 78.56 ± 7.28 and 14.74 ± 1.57 (μM), respectively, comparable with acarbose as a positive control (127.53 ± 1.73 μM); none of these compounds inhibited xanthine oxidase activity. Compounds 1 and 2 are promising candidates for the development of antidiabetic agents from natural sources.

Background: Triple-negative breast cancer (TNBC) is a type of breast cancer whose treatment is significantly more complicated than that for other forms of breast cancer. Chemotherapy is now the mainstay of treatment, but it often causes adverse effects for patients, making nature-based therapies an alternative. Aim of the study: Vernonia amygdalina is a medicinal plant with significant potential to be utilised as a chemo-preventive agent due to its exceptional antioxidant qualities. This research aims to evaluate the anticancer properties of this plant on Hs587t breast cancer cells. Methods: The extract was extracted using ethanol and fractionated with n-hexane and ethyl acetate, then separated by column chromatography. The toxicity was tested on the Hs587t cell line using the micro-tetrazolium technique. The three best subfractions for phytochemical constituents were analyzed using LCHRMS. Flow cytometry was used to analyze the patterns of apoptosis and cell cycle. Results: Cytotoxicity on cells showed that subfractions 1, 2, and 3 (IC50 13.76 ± 0.10, 4.57 ± 0.22, and 9.77 ± 0.27 µg/mL) were the best. This subfraction induces both early and late apoptosis while inhibiting the advancement of the G2−M phase of the cell cycle. Conclusion: The study found that subfractions 1, 2, and 3 of V. amygdalina leaves demonstrated significant anticancer properties against breast cancer. This was observed through their toxicity and ability to induce apoptosis in Hs587t cells. However, additional clinical trial investigations are necessary to obtain more dependable outcomes.

Vernonia amygdalina (VA) leaves contain many potential active ingredients and exhibit diverse pharmacological activities. The antihypertensive, anti-inflammatory, and analgesic effects of VA crude and fraction extracts were carried out using Swiss albino mice models. VAE is considered safe to be administered due to LD50 being greater than 10,000 mg/kg body weight. A dose-dependent increase in antihypertensive, anti-inflammatory, and analgesic activities was observed in both VAE and fractions, similar to the reference drugs. The antihypertensive effect of the VAE 2.0 (2000 mg/kg, SBP: ↓26.05 %, DBP: ↓34.51 %) was nearly equivalent to Captopril (100 mg/kg, SBP: ↓30.28 %, DBP: ↓40.71 %) with no statistically significant difference (p > 0.05). The VAE 1.0 (1000 mg/kg), and EA 30 (30 mg/kg) showed potent anti-inflammatory activity when reducing the total edematous paw volume significantly (p 500 mg/kg), and W 400 (water, 400 mg/kg) fraction extracts showed a potent analgesic effect equivalent to Para 50 (paracetamol 50 mg/kg) for the highest protection (>65 %) against the acetic acid-induced writhing after 35 min. Moreover, cepharanthine, cynaroside, and vernoniosides of VA leaf extract exhibited the highest affinity (>10 kcal/mol) in anti-inflammatory and analgesic targets (iNOS and COX-2) and antihypertensive targets (ACE and β1 adrenoreceptor). Therefore, the crude and fraction extracts of VA leaves from the percolation method and bioactive metabolites are a potential source that can be developed into antihypertensive, anti-inflammatory, and analgesic agents in herbal medicine.

Background: Management of wounds and healing under impaired conditions are the major challenges faced globally by healthcare workers. Phytocompounds which are anti-microbial and capable of modulating inflammation contribute to overall wound healing and regain of the lost structure and function especially in wounds impaired with polymicrobial infection., Methods: An acute cutaneous impaired wound model using adult zebrafish was validated to simulate mammalian wound pathophysiology. This model was used to evaluate phytofractions of Vernonia arborea in the present study, for reduction of infection; myeloperoxidase (MPO) as a marker of infection; neutrophil infiltration and resolution; kinetics of inflammatory cytokines; and wound repair kinetics (viz., nitrite levels and iNoS expression; reepithelisation)., Results: Four fractions which were active in-vitro against five selected wound microbes were shown to reduce ex-vivo microbial bioburden upto 96% in the infected wound tissue. The reduction in CFU correlated with the neutrophil kinetics and MPO enzyme levels in the treated, wound infected zebrafish. Expression of pro-inflammatory cytokines (IL-6 and TNF-α) was downregulated while upregulating anti-inflammatory cytokine (IL-10), and nitric oxide signalling with fourfold increase in iNOS expression. The adult zebrafish wound model could well serve as a standard tool for assessing phytoextracts such as V. arborea for wound healing with anti-microbial properties., (© 2024. The Author(s).)

The high prevalence of nutrition-related health issues has spurred a growing interest in developing healthier snack alternatives. The aim of this study therefore was to develop and assess the sensory as well as the nutritional properties of biscuits supplemented with Vernonia amygdalina leaf (VA) and Heteroclarias spp viscera oil. Four biscuit samples were developed: one without Vernonia amygdalina and fish oil (labeled BWVF) and three others containing increasing quantities of VA (BWVF-1 g, BWVF-2 g, and BWVF-3 g). A commercially purchased conventional biscuit (CB) was utilized as a control. Physicochemical parameters of fish viscera oil, proximate analysis and sensory evaluation using a 9-point hedonic scale was carried out on the formulated biscuits. Results showed that the physicochemical properties of the fish viscera oil were within range recommended for edible oil. Carbohydrate was significantly lower (p < 0.05) in BWVF- 1 g, BWVF- 2 g and BWVF- 3 g in a dose dependent manner compared to the CB, while percentage crude protein, lipid and ash contents increased significantly (p < 0.05) compared to CB. The rating of appearance, texture/consistency, crunchiness, and overall acceptability of the test biscuits were not significantly different (p > 0.05) to CB. However, there was a dose-dependent decrease in the ratings for the taste of BWVF-1 g, BWVF-2 g and BWVF-3g compared to CB and BWVF. In conclusion, Vernonia amygdalina and fish oil-supplemented biscuits have good sensory evaluation and contain nutrients that can contribute to the daily nutritional and caloric needs of consumers.

Objectives: Vernonia amygdalina (VA) is a plant that consumed as vegetable by Indonesians contained numerous secondary metabolites. VA's pharmacological action, including its antioxidant properties, anticancer, antidiabetic, and hepatoprotective. The purpose of this research is to reveal the activity of Vernonia amygdalina . leafs aqueous fraction (VALAF) as a blood pressure-lowering agent in hypertensive model., Methods: Combination of prednisone and NaCl were used as hypertensive inducer. The animals were split into five different groups, normal control group treated with distilled water, treatment VALAF groups with dose of 10; 20 and 40 mg/kg BW respectively, while the last group was treated with captopril at dose of 2.25 mg/kg BW. All animals were given an oral treatment for 15 days. On days 5, 10, and 15, systolic and diastolic blood pressure, heart rate (HR), mean arterial pressure (MAP), and blood flow (BF) were all measured. On days 0 and 15, NO level were assessed. All data were analyzed using two-way ANOVA, and Duncan Multiple Range Test., Results: The V. amygdalina leaf aqueous fraction has blood pressure lowering activity. The blood pressure parameter of the rats treated with VALAF were lower as compared to the normal control group (p<0.05). NO levels in the VALAF group were not significantly higher than in the normal control group (p>0.05). The VALAF 20 give the greatest percentage of decrease in blood pressure, heart rate and blood volume on the 15 th day of examination., Conclusions: These study indicated that V. amygdalina leaf aqueous fraction has the potential to be an alternative therapy for managing blood pressure in hypertensive animal models., (© 2023 Walter de Gruyter GmbH, Berlin/Boston.)

The utilization of natural organic materials as raw materials for standardized medicine, including the use of organic materials in medicine manufacture are currently being encouraged by the Government. Based on the information from Bentian Tribe people, Vernonia arborea leaf or Kutu Bu’ut leaf has the potential to be developed as natural medicine derived from plants. This study explored the potential of V.arborea leaf for its phytochemicals screening by Harborne, Kokate and Senthilmurugan method, antioxidant analysis was evaluated by DPPH radical scavenging assay. Antibacterial analysis was examined using agar well diffusion method against Escherichia coli and Propionibacterium acnes. The results showed that the V.arborea leaf contained alkaloid on n-hexan extract and coumarin on ethyl acetate extract. Ethanol extract from V.arborea leaf contained alkaloid, flavonoid, saponin, tannin, and carbohydrate. Antioxidant activity showed that the highest inhibition by 83% at 50 ppm consentration of ethanol solvent. Antibacterial activity of E. coli and P.acnes showed that the highest inhibition zone by 12 mm and 11 mm at 400 µg/well of ethanol extract. Based on the results, the V.arborea leaf contains natural bioactivity and has potential to be further developed as a natural traditional medicine.

Abstract Today, nanoscience explores the potential of nanoparticles due to their extraordinary properties compared to bulk materials. The synthesis of metal nanoparticles using plant extracts is a very promising method for environmental remediation, which gets global attention due to pollution-led global warming. In the present study, iron nanoparticles (FeNPs) were successfully synthesized by the green method using Vernonia amygdalina plant leaf extract as a natural reducing and capping agent. Biosynthesized FeNPs were characterized with different analytical techniques such as UV–visible, FT-IR, XRD, and SEM. The analysis revealed the formation of amorphous FeNPs with an irregular morphology and non-uniform distribution in size and shape. The average particle size was approximately 2.31 µm. According to the catalytic degradation investigation, the FeNPs produced via the green approach are highly effective in breaking down both CV and MB into non-toxic products, with a maximum degradation efficiency of 97.47% and 94.22%, respectively, when the right conditions are met. The kinetics study exhibited a high correlation coefficient close to unity (0.999) and (0.995) for the degradation of MB and CV, respectively, for the zero-order pseudo-kinetics model, which describes the model as highly suitable for the degradation of both dyes by FeNPs compared to other models. The reusability and stability of biosynthesized nano-catalysts were studied and successfully used as efficient catalysts with a slight decrease in the degradation rate more than four times. The results from this study illustrate that green synthesized FeNPs offer a cost-effective, environmentally friendly, and efficient means for the catalytic degradation of organic dyes.

Vernonia amygdalina, commonly known as bitter leaf, is a widely grown plant in Africa that has several health benefits. This study aimed to compare the antibacterial activities of ciprofloxacin with those of ethanolic and aqueous extracts of Vernonia amygdalina (bitter leaf) on selected bacteria. Swabs were collected from patients presenting with bacterial conjunctivitis at the Eye Clinic, Department of Optometry, Federal University of Technology, Owerri. The samples were transported to the Microbiology Laboratory at the Federal University Teaching Hospital, Owerri, for culturing and identification of microorganisms using standard microbiological methods. The zones of inhibition of ciprofloxacin on selected bacteria isolated from the swab samples were compared with those of the ethanolic and aqueous extracts of Vernonia amygdalina. Four bacteria were isolated from the samples: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Streptococcus pyogenes. At concentrations of 100, 50, 25 and 12.5 mg/ml respectively, there were significant differences (p=0.05) in the zones of inhibition between ciprofloxacin and the ethanolic and aqueous extracts against S. aureus, E. coli, K. pneumoniae and S. pyogenes. With 12.5 mg/ml concentration, the extracts showed no significant differences (p>0.05) in the zone of inhibition. Ciprofloxacin showed significantly higher (p=0.05) zones of inhibition, followed by Vernonia amygdalina ethanolic extract (p=0.05) and Vernonia amygdalina aqueous extract (p=0.05). The zones of inhibition produced by Vernonia amygdalina ethanolic extract on both grampositive and gram-negative bacteria highlight its antibacterial effect and its potential as an alternative treatment option for bacterial infections. [ABSTRACT FROM AUTHOR]

Abstracts: Vernonia amygdalina possess specialized metabolites which have been shown to minimize the risk of human diseases. This study was aimed to evaluate the effect of V. amygdalina extracts on Superoxide dismutase (SOD) and Glutathione s-transferase (GST) on alloxan induced diabetes in Wistar rats. Extraction of the V. amygdalina leaves was done manually and extracts were fractionated using Asymmetric Flow Field Flow Fractionation method. The fractions obtained from the crude V. amygdalina were tested to identify phytochemicals such as alkaloids, glycosides and saponins. Meanwhile, the experimental animals were divided into 2 phases. Phase 1 (non-induced) were grouped into 6 groups (control, crude, metformin, saponin, glycoside and alkaloid) of six animals. Phase 2 were grouped into 5 groups (crude, metformin, saponin, glycoside and alkaloid) of 6 animals which were induced intraperitoneally with Alloxan using injection (Alloxan-Induced). The results for the fasting blood glucose level of treated alloxan-induced diabetic showed a significant increase compared to those in control group (61.33±2.0) of the untreated. In addition, the histopathological analysis of the pancreas showed significant resurgence of destroyed pancreatic islet cells. The findings of the current research showed that Vernonia amygdalina ameliorated the enzymatic activities on alloxan induced diabetes on male Wistar rats.

This study evaluates the therapeutic efficacy and safety of Vernonia Amygdalina extract (VAEE) in treating colorectal cancer (CRC) induced by azoxymethane/dextran sulfate sodium (AOM/DSS) in mice and to elucidate the underlying mechanisms. The study utilized a mouse model of CRC to assess the impact of VAEE on pathological markers, inflammatory mediators, and oxidative stress. Phytochemical profiling of VAEE was conducted using LC-HRMS, focusing on phenolic compounds. The extract's antioxidant activity was quantified, and its safety profile was verified through acute toxicity assessments. VAEE significantly mitigated the AOM/DSS-induced reduction in colon length and weight loss in a dose-dependent manner. The treatment decreased pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) while increasing the anti-inflammatory cytokine IL-10. VAEE also demonstrated strong antioxidant activity and did not adversely affect blood, kidney, or liver biomarkers, confirming its non-toxicity. Protein-protein interaction analysis suggested VAEE's role in inhibiting inflammatory markers, including COX-2, IL-18, and NF-kB. Molecular docking results supported the extract's affinity for these proteins, indicating potential as a COX-2 inhibitor. VAEE exhibits potent anti-inflammatory, antioxidative, and anticarcinogenic properties against CRC, with a favorable safety profile. These findings advocate for the integration of VAEE as a novel therapeutic agent in CRC treatment. Further investigations into its molecular mechanisms and long-term effects are warranted to facilitate clinical translation.

Introduction: Moringa oleifera, Vernonia amygdalina, and Centella asiatica are herbs used in traditional Chinese medicine and are commonly used in Southeast Asian countries to treat a variety of diseases. The experimental evidence showed that M. oleifera leaves, V. amygdalina leaves, and C. asiatica had the effect of supporting their treatment of diabetes. This study investigated the anti-hyperglycemic effect of a novel herbal formula from M. oleifera leaf, V. amygdalina leaf, and C. asiatica aerial part extracts. Methods: The extract was examined for anti-hyperglycemic capacity in oral glucose tolerance test in normal mice. To confirm their role in a higher model, the extract was studied in the streptozotocin (STZ)-induced hyperglycemic model. Plasma glucose concentration, body weight, organ weight, malondialdehyde, glutathione in liver and kidneys, and pancreatic histology were also evaluated. Besides, in vitro properties of the extract were tested including α-amylase and α-glucosidase inhibitory, and antioxidant activities. Results: After 7-day of treatment, the extract showed strong anti-hyperglycemic activity in the STZ-induced hyperglycemic mice, plasma glucose levels were reduced by 48.75 % and 54.13 % in the group administered with 200 mg/kg and 400 mg/kg of the extract, respectively, compared with the hyperglycemic control. The extract had the effect to protect the liver and kidneys against oxidation stress by enhancing glutathione levels in the liver and decreasing malondialdehyde levels in the liver and kidneys. The pancreatic β-cell protection was also observed by improving the size and number of pancreatic islets by the extract compared to the hyperglycemic control. The extract showed a significant α-amylase and α-glucosidase inhibitory activities, and antioxidant activity through DPPH and ABTS quenching and reducing power abilities. Discussion: These results demonstrated remarkable potential of the combined extract of M. oleifera leaves, V. amygdalina leaves, and aerial part of C. asiatica in the management of diabetes.

Context: Vernonia amygdalina Delile (VAD) is known as a potential plant with a wide variety of medicinal properties, including anticancer. Aims: To evaluate the combination effect of VAD extract with cisplatin (CIS) against PANC-1 cells, focusing on cell cycle arrest and apoptosis activities. Methods: This study is an experimental study using PANC-1 cells as objects. The phytochemical compounds were analyzed with LC-MS/MS. The cytotoxic activity of extracts and their combinations was determined using the MTT assay method in the PANC-1 cell line. Apoptosis, cell cycle arrest, and PI3K/mTOR profiles were analyzed with flow cytometry. Immunocytochemistry was used to determine Bcl-2, Cyclin D1, and p53 expression. Results: The phytochemicals found were five compounds with retention times of 8.96, 9.85, 10.52, 12.59, and 10.36. The results of the MTT assay showed that the IC50 values of extract and CIS were 21.83 ± 0.46 µg/mL and 3.02 ± 0.44 µg/mL, respectively. The combination index (CI) value of extract and CIS had a synergistic effect. Combination extract and CIS induced early and late apoptosis, inhibited cell cycle progression on the G1 phase, inhibited Bcl-2 and cyclin D1 expression, induced p53 expression, and inhibited PI3K and mTOR expression. Conclusions: The combination of extract and CIS showed anticancer activity against PANC-1 cells through induction of apoptosis and cell cycle arrest via inhibition of PI3K and mTOR expressions.

Diabetes mellitus (DM), a complex heterogeneous metabolic disorder characterized by a defect in the function of insulin, is on the rapid rise globally. Sustained hyperglycemia which is a major sign of DM is linked to the generation of reactive oxygen species which promotes adverse complications of the disorder. Traditional herbal treatment of DM is a common practice in Africa and other tropical parts of the world. Vernonia amygdalina (VA), one of the highly researched species in the Asteraceae family, has proven to possess potent antidiabetic properties. Several phytochemicals identified in multiple extracts from VA are purported to be responsible for the antidiabetic potential of the plant. In this review, we discuss the therapeutic potential of VA in diabetes and its associated complications. We appraise the current evidence and further suggest potential areas that could be effectively exploited in future VA research on diabetes., Competing Interests: The authors declare that there are no conflicts of interest., (Copyright © 2023 Du-Bois Asante and Gideon Akuamoah Wiafe.)

RESUMO As propriedades antifúngicas de 2 extratos, obtidos a partir de caules de Vernonia scorpioides (Lam.) Pers., foram testadas para Penicillium citrinum, produtor de micotoxina nefrotóxica. Os extratos foram utilizados em ensaio de antibios e pelo método de difusão em agar, nas concentrações de 1 mg. 3 mg e 5 mg para cada 100 l de diluente, originando halos de inibição com 40, 50 e 80 mm, respectivamente. Tal resultado sugeriu ação fungicida, uma vez que não se conseguiu, à partir dos tratamentos, realizar-se subcultivos em meio Czapeck-Dox isento de inibidores. Foi avaliada a toxidez desses extratos ativos para camundongos albinos, aos quais ministrou-se uma dose cem vezes superior à utilizada in vitro. Não houve interferência no estado geral, ou ganho de peso, dos animais tratados durante 6 dias subsequentes a administração oral dos extratos. Através dos espectros no infravermelho dos extratas ativos, observou-se a existência de sinais, característicos do estiramento de carbonilas lactônicas (com absorção entre 1750 cm-1 e 1770 cm-1).

Every year, metabolic syndrome and cardiorenal diseases cause many deaths worldwide. African bitter leaf (Vernonia amygdalina) is known for its numerous therapeutic effects. Potentially, it can lower plasma lipid and glucose levels, which, in turn, may improve the condition of patients with the abovementioned diseases. This research featured the antihyperlipidemic and antihyperglycemic effects of methanol extract of V. amygdalina in an animal model of metabolic syndrome. Twenty albino rats were divided into four groups. Groups A to C were orally administered with ghee (3 mL/kg) + high-cholesterol diet (500 mg/kg) + high-sugar diet (10 mL/kg) to induce metabolic syndrome. Group A served as negative control and received no treatment with bitter leaf methanol extract. Groups B and C received 200 and 400 mg/kg of V. amygdalina methanol extract, respectively. Group D received no administration. The cardiorenal injuries and alterations in blood lipids and sugar levels were assessed via various biochemical analyses. The combination of ghee + high-cholesterol diet + high-sugar diet triggered a significant elevation of creatine kinase myocardial band, lactate dehydrogenase, aspartate aminotransferase, triglycerides, total cholesterol, low density lipoprotein-cholesterol, glucose, urea, creatinine, and potassium levels. The histopathological results agreed with the biochemical findings. However, the treatment with 200 and 400 mg/kg of V. amygdalina methanol extract was able to inhibit the adverse alterations causing a dosedependent significant antihyperlipidemic and antihyperglycemic effect (p < 0.05). Bitter leaf (V. amygdalina) demonstrated cardiorenal protective effects and may be used to manage metabolic syndrome.

RESUMO Com o objetivo de se verificar uma possível relação entre: atividade biológica de Vernonia scorpioides, planta medicinal de potente atividade antifúngica, e níveis de alguns elementos nutricionais, foram realizadas dosagens de N-amino, N- total, potássio e fósforo em raízes, caules e folhas através de processos analíticos clássicos. Nas raízes, onde é obtido o extrato mais ativo, foram encontrados os menores teores de nutrientes, sugerindo uma relação inversamente proporcional a atividade antifúngica para ela descrita.

Vernonia elaeagnifolia is an evergreen creeping plant belonging to the sunflower family. It has been investigated for various medicinal properties, but there is limited information on its antioxidant and anticancer properties. This study therefore aims to examine the antioxidant and anticancer potentials of the ethanol extract of Vernonia elaeagnifolia leaves (VEE). VEE was obtained by maceration in 96% ethanol. Preliminary phytochemical screening was done according to standard method. Antioxidant activity of VEE was assessed using the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+) radical scavenging assay. Cytotoxic effect against 4T1 and MCF7 breast cancer cells, as well as Vero non-cancerous cells was examined by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Phytochemical screening of VEE reveals the presence of alkaloids, flavonoids, saponins, phenolics, and tannins. VEE exhibited modest antioxidant activity with IC50 of 147.28 μg/mL, compared to ascorbic acid which had an IC50 of 5.43 μg/mL. VEE possessed moderate cytotoxic effect against 4T1, and MCF7, and a mild cytotoxic effect against Vero cells with IC50 of 165.50, 185.68, and 299.66 μg/mL, respectively. VEE was less potent than Doxorubicin which exhibited IC50 values of 0.44, 2.09, and 4.51 μg/mL, against 4T1, MCF7, and Vero cells, respectively. The selectivity index of VEE was 1.6 - 1.8, indicating limited selectivity for cancer cells. The findings from this study have shown the potentials of Vernonia elaeagnifolia leaves as an antioxidant and a mild cytotoxic agent against breast cancer cells. [ABSTRACT FROM AUTHOR]

Introduction:Vernonia anthelmintica (L.) Willd. is a traditional treatment for vitiligo in Xinjiang. However, its therapeutic mechanism remains unclear owing to its complex composition and limited research on its chemical profile.Methods: We employed a targeted metabolome approach, combining selective reaction monitoring/multiple response monitoring (SRM/MRM) with high-performance liquid chromatography and MRM mass spectrometry to quantitatively analyze the flavonoid constituents of Vernonia anthelmintica. We also used network pharmacology and molecular docking to identify potential vitiligo-linked compounds and targets of V. anthelmintica seeds. Additionally, we assessed HaCaT cell proliferation by AAPH-induced, alongside changes in SOD activity and MDA content, following treatment with V. anthelmintica components. Finally, flow cytometry was used to detect apoptosis and ROS levels.Results and Discussion: We identified 36 flavonoid compounds in V. anthelmintica seeds, with 14 compounds exhibiting druggability. AKT1, VEGFA, ESR1, PTGS2, and IL2 have been identified as key therapeutic target genes, with PI3K/AKT signaling being an important pathway. Notably, kaempferol, one of the identified compounds, exhibited high expression in network pharmacology analysis. Kaempferol exhibited a strong binding affinity to important targets. Further, kaempferol enhanced HaCaT cell viability, inhibited apoptosis, reduced MDA levels, suppressed ROS activity, and upregulated SOD activity, increase the expression of cellular antioxidant genes, including HO-1, GCLC, GCLM, Nrf2, NQO1 and Keap1, providing significant protection against oxidative stress damage in vitro. Here, we present the first comprehensive study integrating SRM/MRM approaches and network analysis to identify active flavonoid compounds within V. anthelmintica (L.) Willd. Moreover, we revealed that its active ingredient, kaempferol, offers protection against AAPH-induced damage in keratinocytes, highlighting its potential as a clinical resource.

Vernonia amygdalina is a perennial shrub that belongs to the family Asteraceae. The herb is an indigenous African plant that grows in most parts of sub-Saharan Africa. It is probably the most used medicinal plant in the genus Vernonia. Previous studies on the traditional medicinal value, nutritional composition, classes of phytochemical or compound isolation, and evaluation of their pharmacology activity are numerous. This provokes us to review and provide up-to-date evidence-based information on the study plant. A systematic online search using the databases of Google Scholar, PubMed, Science Direct, Wiley, Elsevier and Sci-Hub was carefully applied, using some important key words to get appropriate information. The leafy part of Vernonia amygdalina contributes greatly to the nutritional requirements for human health and to food security since it contains enough concentrations of proximate composition, minerals, and vitamins. The plant parts are used in traditional medicine for many human and animal healthcare purposes, including diarrhea, diabetes, wound healing, tonsillitis, evil eye, retained placenta, headache, eye disease, intestinal parasite, bloating, hepatitis, toothache, anthrax, malaria, urine retention, gastritis, stomach disorders, and snake bites. The chemical analysis revealed the presence of flavonoids, alkaloids, saponins, tannins, triterpenoids, sesquiterpene lactones, steroids, cardiac glycosides, oxalates, phytates, cyanogenic glycosides, and phenols. Additionally, various compounds such as vernolide, luteolin, vernodalol, vernoamyoside A, vernoamyoside B, isorhamnetin, glucuronolactone, and 1-Heneicosenol O-β-D-glucopyranoside were isolated. Some of the isolated compounds pharmacological activity was evaluated against some diseases and showed antioxidant, antibacterial, antifungal, antihelmintic, anticancer, and anti-inflammatory potencies. Thus, the review provides comprehensive information about ethnomedicinal value, nutritional composition, isolated classes of phytochemicals, and compounds, including an evaluation of the pharmacological activity of the isolated compounds of Vernonia amygdalina. A review with this much information could be extremely valuable for future research on developing innovative nutraceutical products.

Four polyoxygenated stigmastanes ( 1 - 4 ) alongside known analogues ( 7 - 8 ) and flavonoids ( 5 - 6 ) were isolated from a dichloromethane/methanol (1:1, v / v ) extract of the whole plant of Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae). Their structures were determined by means of spectroscopic and spectrometric analysis. The relative stereochemistry of the new compounds was established and confirmed via biosynthesis evidence and cyclization of 1 under acidic conditions. A plausible biosynthetic pathway to the new compounds and the chemophenetic significance of the isolated constituents were also discussed. The crude extract, fractions, and compounds ( 1 - 3 ) were assessed for their antibacterial activity against five highly prevalent bacterial strains. The fractions and compounds showed low to moderate activity with minimal inhibitory concentrations (MICs) > 125 µg/mL.

Phytochemical investigation of the whole plants of Vernonia gratiosa Hance. led in the isolation and identification of two new stigmastane-type steroidal glucosides ( 1 - 2 ), namely vernogratiosides A ( 1 ), and B ( 2 ). Their chemical structures were fully elucidated based on 1 D/2D NMR spectroscopic, HR-ESI-MS data analyses, and by producing derivatives by chemical reactions. The binding potential of the isolated compounds to replicase protein - main protease of SARS-CoV-2 were examined using the molecular docking simulations. Our results show that the isolated steroidal glucosides ( 1 - 2 ) bind to the substrate-binding site of SARS-CoV-2 main protease with binding affinities of -7.2 and -7.6 kcal/mol, respectively, as well as binding abilities equivalent to N3 inhibitor that has already been reported (-7.5 kcal/mol).