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Cardiovascular disease is the leading cause of death, medical complications, and healthcare costs. Although recent advances have been in treating cardiovascular disorders linked with a reduced ejection fraction, acutely decompensate cardiac failure remains a significant medical problem. The transient receptor potential cation channel (TRPC6) family responds to neurohormonal and mechanical stress, playing critical roles in cardiovascular diseases. Therefore, TRP C6 channels have great promise as therapeutic targets. Numerous studies have investigated the roles of TRP C6 channels in pain neurons, highlighting their significance in cardiovascular research. The TRPC6 protein exhibits a broad distribution in various organs and tissues, including the brain, nerves, heart, blood vessels, lungs, kidneys, gastrointestinal tract, and other bodily structures. Its activation can be triggered by alterations in osmotic pressure, mechanical stimulation, and diacylglycerol. Consequently, TRPC6 plays a significant role in the pathophysiological mechanisms underlying diverse diseases within living organisms. A recent study has indicated a strong correlation between the disorder known as TRPC6 and the development of cardiovascular diseases. Consequently, investigations into the association between TRPC6 and cardiovascular diseases have gained significant attention in the scientific community. This review explores the most recent developments in the recognition and characterization of TRPC6. Additionally, it considers the field's prospects while examining how TRPC6 might be altered and its clinical applications., Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Elsevier Inc. All rights reserved.)

This study utilizes artificial intelligence and statistical modelling to optimize the operating parameters of a carbon-based electro-Fenton process for purifying model dye (RB19)-contaminated wastewater. Multilevel experimental Box-Behnken and uniform deisgns (BBD, UD) with four variables were analysed using polynomial regression analysis (PRA) and artificial neural networks (ANN), while the process optimisation was done using desirability function. For the given testing range but different design matrices and runs, both designs predicted a maximum RB19 removal (RB19-RR) of 90 ± 2.1% at lowest energy consumption (EC) of 0.44 ± 2.5 Wh, when voltage, Na 2 SO 4 , FeSO 4 , and time were maintained as follows: 4-5.3 V, 7-11 mM, 0.4-0.6 mM, and 35-40 min, respectively. All the design-model combinations portrayed the similar senitivity analyses, revealing that RB19 degradation and EC are primarily influenced by electrolysis time and voltage. The performance assessment demonstrated that all the design-model combinations also excellently predicted for unseen conditions as the maximum root mean squared error (RMSE) value for RB19-RR was 4.07, while it was 0.072 for EC, however, BBD-ANN performance proved to be slightly better than others. Having ∼57% less experimentation, UD based models managed to accurately predict the results for unseen conditions as the statistical errors were quite insignificant, even in some cases, RMSE found to be less for UD compared to BBD, elucidating the potential of uniform design as an alternative of conventional factorial designs. Nevertheless, the prediction accuracy is also dependent on modelling approach, as in some cases ANN failed to predict the response precisely specially when dealing with small data. Furthermore, techno-economic evaluation results spell out the efficacy of carbon felt based enhanced electro-Fenton process as promising environmental remediation technology and highlight its practical implication from view of operational cost., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The tumor microenvironment (TME), which includes both cellular and non-cellular elements, is now recognized as one of the major regulators of the development of primary tumors, the metastasis of which occurs to specific organs, and the response to therapy. Development of immunotherapy and targeted therapies have increased knowledge of cancer-related inflammation Since the blood-brain barrier (BBB) and blood-cerebrospinal fluid barrier (BCB) limit immune cells from entering from the periphery, it has long been considered an immunological refuge. Thus, tumor cells that make their way "to the brain were believed to be protected from the body's normal mechanisms of monitoring and eliminating them. In this process, the microenvironment and tumor cells at different stages interact and depend on each other to form the basis of the evolution of tumor brain metastases. This paper focuses on the pathogenesis, microenvironmental changes, and new treatment methods of different types of brain metastases. Through the systematic review and summary from macro to micro, the occurrence and development rules and key driving factors of the disease are revealed, and the clinical precision medicine of brain metastases is comprehensively promoted. Recent research has shed light on the potential of TME-targeted and potential treatments for treating Brain metastases, and we'll use that knowledge to discuss the advantages and disadvantages of these approaches., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Khan, Khan, Sala, Khan, Ud Din, Khan, Hassan, Khan and Liu.)

Acquired amegakaryocytic thrombocytopenic purpura (AATP) is a rare bone marrow disorder characterized by either a marked decrease or a complete absence of megakaryocytes with the preservation of all other cell lines. To date, more than 60 cases of AATP have been reported in the literature. Due to the rarity of this disease, no standard treatment guidelines have been established, and therapy is based on a handful of case studies and expert opinions. Herein, we provide a comprehensive review of currently utilized therapeutic options for AATP.

Legumain (LGMN) has been demonstrated to be overexpressed not just in breast, prostatic, and liver tumor cells, but also in the macrophages that compose the tumor microenvironment. This supports the idea that LGMN is a pivotal protein in regulating tumor development, invasion, and dissemination. Targeting LGMN with siRNA or chemotherapeutic medicines and peptides can suppress cancer cell proliferation in culture and reduce tumor growth in vivo . Furthermore, legumain can be used as a marker for cancer detection and targeting due to its expression being significantly lower in normal cells compared to tumors or tumor-associated macrophages (TAMs). Tumor formation is influenced by aberrant expression of proteins and alterations in cellular architecture, but the tumor microenvironment is a crucial deciding factor. Legumain (LGMN) is an in vivo-active cysteine protease that catalyzes the degradation of numerous proteins. Its precise biological mechanism encompasses a number of routes, including effects on tumor-associated macrophage and neovascular endothelium in the tumor microenvironment. The purpose of this work is to establish a rationale for thoroughly investigating the function of LGMN in the tumor microenvironment and discovering novel tumor early diagnosis markers and therapeutic targets by reviewing the function of LGMN in tumor genesis and progression and its relationship with tumor milieu., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Khan, Khan, Khan, Ud Din, Khan, Khan and Liu.)

Rational design and synthesis of multifunctional electrocatalysts with high electrochemical activity and low cost are significantly important for new-generation lithium-sulfur (Li-S) batteries. Herein, N-doped FeP nanospheres decorated N doped carbon matrix is successfully synthesized by facile one-pot pyrolysis and in-situ phosphorization technique to mitigate the conversion kinetics and suppress the shuttle effect. The large specific surface area with mesopores can incorporate up to 81.5% sulfur, with the conductive carbon and nitrogen co-matrix providing Li + /e - passage and fastening the redox kinetics. The remarkable adsorption properties and the electrocatalytic activity through physical confinement and chemical immobilization is thoroughly verified. Consequently, the FeP/CN@S deliver a high reversible capacity of 1183 mAh g -1 at 0.1C compared to Co/P/CN@S (961 mAh g -1 ); whereas, at 1C, a negligible decay rate of 0.04% is observed for 1000 cycles, possessing outstanding cycling stability and rate capability. Hence, the cost-effective in-situ phosphorization strategy to synthesize FeP/CN@S as an efficient nanoreactor is constructive to be applied in Li-S batteries., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Severe haemolytic anaemia is a rare complication of haemodialysis that is often difficult to recognize, especially when there are other potential differential diagnoses. Here, we present the case of 19-year-old man on haemodialysis who developed severe haemolytic anaemia while recovering from acute renal failure secondary to rhabdomyolysis. Other causes of haemolytic anaemia such as thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome were ruled out. As his blood counts were dropping on days following haemodialysis, haemolysis secondary to the mechanical sheering effect of the catheter was considered and his haemodialysis catheter was exchanged, which led to the resolution of anaemia., Learning Points: Haemodialysis-related haemolysis can be due to the mechanical sheering effect of the catheter/tubing, and overheating or contamination of the dialysate fluid.Most cases of catheter or faulty tubing-related haemolysis need a high level of suspicion for diagnosis and normally resolve with correction of the underlying mechanical issue.Due to the rarity of the phenomenon, it is very important to rule out other life-threatening causes of haemolytic anaemia, such as disseminated intravascular coagulation and thrombotic thrombocytopenic purpura., Competing Interests: Conflicts of Interests: The authors declare there are no competing interests., (© EFIM 2021.)

A 44-year-old woman with no significant medical history presented with a 3-week history of high-grade fevers, fatigue and shortness of breath. Laboratory investigation was significant for lymphopenia and thrombocytopenia which progressively worsened during her hospital stay, along with new-onset anaemia, and elevated ferritin, transaminase and triglycerides. A computerized tomography (CT) scan of the abdomen revealed retroperitoneal lymphadenopathy. A bone marrow biopsy confirmed the diagnosis of haemophagocytic lymphohistiocytosis (HLH). Extensive infectious work-up revealed high IgG titres for Bartonella henselae and Coxiella burnetii . Interestingly, the left supraclavicular node was negative for both microbes by polymerase chain reaction (PCR), but the biopsy revealed anaplastic large T-cell lymphoma., Learning Points: Haemophagocytic lymphohistiocytosis (HLH) is an important differential diagnosis to consider for fever of unknown origin in adults, especially in the setting of pancytopenia and hyperferritinaemia.Q fever resulting from Coxiella burnetii can cause HLH and is also postulated to increase the risk of lymphoma. Bartonella henselae infection can also trigger HLH, but the risk of lymphoma following infection by B. henselae is unknown., Competing Interests: Conflicts of Interests: The Authors declare that there are no competing interests., (© EFIM 2020.)

Designing a self-assembling structure for a Pt-based catalyst offers a great opportunity to enhance the electrocatalytic performance and maximize the use of precious metals. Herein, we report an etching method based on thermal treatment for the removal of less active metals from Pt-based alloys for the enhancement of the oxygen reduction reaction. PtPdAg nanostructures' self-assembly can be easily controlled to the dimer stage or nanowires by stirring the nanoparticles in formamide with or without potassium iodide under heat for specific times. Thus oxygen reduction reaction-favoring PtPdAg hollow nanoparticle, nanodimer and nanowire catalysts are synthesized, all of which have been demonstrated to be promoting factors for the ORR. In a Pt-based catalyst, the arrangement and configuration of the surface or topmost few layer atoms influence the adsorption of oxygen and activation for ORR. The PtPdAg dimer catalyst shows excellent ORR activity compared to other PtPdAg nanostructures and commercial Pt/C i.e. 7.2 times higher specific activity and 4.1 times higher mass activity. We further carried out DFT calculations and from the results, we conclude that the most chemically inequivalent structure such as PtPdAg/C nanodimer alloys possesses the weakest oxygen binding energy.

Two-dimensional nanomaterials, particularly multimetallic nanosheets with single or few atoms thickness, are attracting extensive research attention because they display remarkable advantages over their bulk counterparts, including high electron mobility, unsaturated surface coordination, a high aspect ratio, and distinctive physical, chemical, and electronic properties. In particular, their ultrathin thickness endows them with ultrahigh specific surface areas and a relatively high surface energy, making them highly favorable for surface active applications; for example, they have great potential for a broad range of fuel cell applications. First, the state-of-the-art research on the synthesis of nanosheets with a controlled size, thickness, shape, and composition is described and special emphasis is placed on the rational design of multimetallic nanosheets. Then, a correlation is performed with the performance of multimetallic nanosheets with modified and improved electrochemical properties and high stability, including for the oxygen reduction reaction (ORR), hydrogen evolution reaction (HER), formic acid oxidation (FAO), methanol oxidation reaction (MOR), ethanol oxidation reaction (EOR), and methanol tolerance are outlined. Finally, some perspectives and advantages offered by this class of materials are highlighted for the development of highly efficient fuel cell electrocatalysts, featuring low cost, enhanced performance, and high stability, which are the key factors for accelerating the commercialization of future promising fuel cells.

A hierarchical excavated iron-nitrogen codoped mesoporous carbon (Ex-FeN-MC) electrocatalyst has been successfully synthesized by pyrolysis of the unique ureidopyrimidinone containing organic precursor MAMIS by using silica as the mesoporous template and iron nitrate as the source of iron. The selected organic precursor has multifunctionalities with diverse distribution of nitrogen and oxygen species which constitute interesting features to prepare the Ex-FeN-MC electrocatalyst. The as-prepared Ex-FeN-MC material shows unexpectedly higher catalytic activities towards ORR/OER performances with an overpotential of 250 mV for OER in alkaline solution at a current density of 10 mA cm -2 , while the ORR activity is almost similar to that of commercial Pt/C. It is believed that the excellent electrochemical performance results from the synergic contribution of the hierarchical uniform mesoporous structure, evenly distributed iron nanoparticles and high density of Ex-FeN-MC catalytically active sites. The presence of the hierarchical uniform mesoporous structure considerably accelerates the mass transfer and efficiently promotes the full utilization of active sites. Furthermore, it also possesses amorphous carbon in coexistence with lattice graphitic carbon which also enhanced the ORR/OER performances. Our research findings can surely provide a new insight to design highly active ORR/OER electrocatalysts by utilizing more novel organic precursors to provide interesting characteristics to electrocatalysts, as well as open up a route for constructing unique multifunctional materials with mesoporous architectures for electrocatalysis and supercapacitor applications.

Development of efficient electrocatalysts for oxygen evolution reaction (OER) is of great significance for future renewable energy applications. Herein, efficient OER electrocatalysts based on iron hydroxide-modified nickel hydroxylphosphate (NiPO/Fe(OH) x ) single-wall nanotubes (SWNTs) have been prepared by a facile stepwise surfactant-free solvothermal strategy, which possess diameters of about 6 nm and lengths of about several micrometers. Benefiting from the synergistic effect between iron hydroxides and NiPO SWNTs, the as-prepared NiPO/Fe(OH) x SWNTs exhibit higher OER activity than primary NiPO SWNTs. Furthermore, the OER activity with different Fe contents displays a volcano-type shape, and the optimized NiPO/Fe(OH) x SWNTs present excellent activity with a low overpotential of 248 mV to deliver a current density of 10 mA cm -2 and 323 mV to achieve a large current density of 100 mA cm -2 , as well as a remarkably low Tafel slope of 45.4 mV dec -1 in 1 M KOH electrolyte. The present work provides valuable insights to improve the OER performance by rational surface modification.

Ultrathin nanocrystals generally provide a remarkable catalytic performance due to their high specific surface area and exposure of certain active sites. However, deactivation caused by growth and gathering limits the catalytic application of ultrathin nanocrystals. Here we report Ag nanocrystal-decorated polyoxometalate (Ag-POM) single-walled nanotubes assembled via a concise, surfactant-free soaking method as a new kind of well-defined core-sheath nanoreactor. The diameter of Ag nanocrystals inside polyoxometalate nanotubes can be controlled via simply adjusting the reactant concentration. Ag-POM provided outstanding oxidative desulfurization (ODS) catalytic performance for aromatic sulfocompounds at room temperature. It was suggested that Ag nanocrystals decorated on the inner surface played a key role in adjusting the electronic distribution and enhancing the catalytic activity. The as-prepared Ag-POM nanotubes are promising candidate catalysts with enhanced performance for practical catalytic applications in the gasoline desulfurization industry.

Mechanistic control is a powerful means for manufacturing specific shapes of metal nanostructures and optimizing their performance in a variety of applications. Thus, we successfully synthesized multimetallic nanoplates (PtAgBiCo and PtAgBi) by combining the concepts of crystal symmetry, oxidative etching and seed ratio, and tuned their activity, stability and methanol tolerance, as well as Pt utilization, for the oxygen reduction reaction in direct methanol fuel cells. Systematic studies reveal that the formation of PtAgBiCo triangular nanoplates with a high morphological yield (>90%) can be achieved by crystallinity alteration, while electrochemical measurements indicate that the PtAgBiCo nanoplates have superior electrocatalytic activity towards the oxygen reduction reaction. The specific and mass activity of the PtAgBiCo nanoplates are 8 and 5 times greater than that of the commercial Pt/C catalyst, respectively. In addition, the tetrametallic PtAgBiCo nanoplates exhibit a more positive half-wave potential for the oxygen reduction reaction and possess an excellent methanol tolerance limit compared with the commercial Pt/C catalyst.

Porous PtCuBiMn nanosheets are developed with a thickness of ≈3-4 nm. The specific and mass activities of these nanosheets are ten and seven times greater than that of commercial Pt/C catalyst, respectively. PtCuBiMn nanosheets demonstrate superior catalytic performance for the oxygen reduction reaction, as well as resistance to methanol cross-over effects, suggesting that it is a promising catalyst for direct methanol fuel cells., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Highlights: Various bismuth-based materials used in potassium-ion batteries (PIBs) anode are classified and overviewed, and the structure and potassium storage mechanism of various materials are discussed. The advantages and challenges of different PIBs anode materials are pointed out, and the existing modification strategies to improve potassium storage are summarized. The promising research directions of bismuth-based anode materials are proposed. Potassium-ion batteries (PIBs) are considered as a promising energy storage system owing to its abundant potassium resources. As an important part of the battery composition, anode materials play a vital role in the future development of PIBs. Bismuth-based anode materials demonstrate great potential for storing potassium ions (K+) due to their layered structure, high theoretical capacity based on the alloying reaction mechanism, and safe operating voltage. However, the large radius of K+ inevitably induces severe volume expansion in depotassiation/potassiation, and the sluggish kinetics of K+ insertion/extraction limits its further development. Herein, we summarize the strategies used to improve the potassium storage properties of various types of materials and introduce recent advances in the design and fabrication of favorable structural features of bismuth-based materials. Firstly, this review analyzes the structure, working mechanism and advantages and disadvantages of various types of materials for potassium storage. Then, based on this, the manuscript focuses on summarizing modification strategies including structural and morphological design, compositing with other materials, and electrolyte optimization, and elucidating the advantages of various modifications in enhancing the potassium storage performance. Finally, we outline the current challenges of bismuth-based materials in PIBs and put forward some prospects to be verified. [ABSTRACT FROM AUTHOR]

The tumor microenvironment (TME) is integral to cancer progression, impacting metastasis and treatment response. It consists of diverse cell types, extracellular matrix components, and signaling molecules that interact to promote tumor growth and therapeutic resistance. Elucidating the intricate interactions between cancer cells and the TME is crucial in understanding cancer progression and therapeutic challenges. A critical process induced by TME signaling is the epithelial-mesenchymal transition (EMT), wherein epithelial cells acquire mesenchymal traits, which enhance their motility and invasiveness and promote metastasis and cancer progression. By targeting various components of the TME, novel investigational strategies aim to disrupt the TME's contribution to the EMT, thereby improving treatment efficacy, addressing therapeutic resistance, and offering a nuanced approach to cancer therapy. This review scrutinizes the key players in the TME and the TME's contribution to the EMT, emphasizing avenues to therapeutically disrupt the interactions between the various TME components. Moreover, the article discusses the TME's implications for resistance mechanisms and highlights the current therapeutic strategies toward TME modulation along with potential caveats. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Moyamoya disease (MMD) is a chronic cerebrovascular disorder increasing stroke risk. This study presents a four‐year‐old MMD patient, discussing her multidisciplinary care plan including dental, behavioral, and medical management. It emphasizes the need for awareness among oral physicians and suggests further research due to the rising prevalence of MMD. Moyamoya disease (MMD) is a chronic cerebrovascular disorder that narrows major blood vessels in the brain, reduces blood flow, and increases the risk of stroke. The purposes of this study were to: (1) present the case of a four‐year‐old patient diagnosed with MMD; (2) discuss important aspects of her multidisciplinary care plan involving dental, behavioral, and medical management; and (3) suggest approaches and recommendations for health care providers. A four‐year‐old Middle Eastern female diagnosed with MMD, neurogenic bladder, chronic constipation, hyperactivity, and delayed milestones presented to the dental clinic with her parents seeking dental care. The child was referred by a pediatric neurologist for dental care. In the present case, there were no specific oral manifestations. However, oral physicians should be aware of possible behavioral management modalities when providing dental care to pediatric patients, including those diagnosed with MMD. [ABSTRACT FROM AUTHOR]

Bismuth microspheres@N-doped porous carbon anode (Bi@NPC-2) is rational designed and successfully prepared by an easy one-pot heat treatment process. The composite demonstrated superior sodium storage performance, including excellent cyclic stability of 326.4 mAh g−1 after 4000 cycles at 5 A g−1 with a capacity retention of 95% and ultra-high rate capability of 286.7 mAh g−1 at a high current rate of 40 A g−1. This excellent performance is mainly due to the unique material design, the three-dimensional porous structure not only helps to mitigate the volume expansion of bismuth, but also shortens the diffusion path of sodium ions. In addition, nitrogen doping improves the conductivity of the electrode and provides more active sites for sodium storage. More importantly, the preparation process is simple, and the raw materials used are cheap, thus the Bismuth microspheres@N-doped porous carbon composite shows important practical large-scale applications. This one-step sintering method can also be applied to other alloy-based sodium storage materials. [ABSTRACT FROM AUTHOR]

Seawater desalination is increasing due to the global water crisis, and velocity caps are widely used at intake entrances to protect marine life and facilitate the required flow. Despite various investigations of seawater intake, a lack of precise research on the discharge coefficient of these structures is evident. The discharge characteristics of seawater intake are investigated both analytically and experimentally. Results show that the circle velocity caps have higher capacity than square ones, about 2% to 3.5%. Also, the Froude number of approach flow and the area of the velocity caps are the two parameters with the greatest impact on discharge coefficients. However, the height of caps and the number of separator blades have the least effect. Furthermore, some equations of discharge coefficients are developed based on machine-learning techniques with appropriate accuracy. Additionally, the Taguchi method, evaluated as an economical approach, significantly reduces the number of tests while the accuracy of results decreases. [ABSTRACT FROM AUTHOR]

Pakistan has a large population of more than 150 million people with an overall carrier frequency of approximately 5.6% for beta-thalassemia. Punjab is the largest province of the country having more than 50% of the population. The state of beta-thalassemia is alarming as consanguinity is very high (>81%) and the literacy rate is low in South Punjab. A thalassemia prevention program is the need of the hour in this part of Pakistan. In this study, we initiated awareness, screening, and characterization of the mutations causing beta-thalassemia as well as a genetic counseling program mainly in the districts of Faisalabad and D.G. Khan to establish prenatal diagnosis, a facility previously unavailable in this region for disease prevention. A total of 248 unrelated transfusion-dependent children and the available members of their families were screened to characterize the mutations and identify the carriers. Genetic counseling was provided to these families and prenatal diagnosis offered. In the samples analyzed, 11 beta-thalassemia mutations and three hemoglobin variants were detected mainly by using the Monoplex and Multiplex ARMS-PCR. First-trimester prenatal diagnosis was carried out through chorionic villus sampling (CVS) in seven pregnancies at risk. As a result of our campaign, 145 carrier couples planning to have more children gave their consent to have retrospective prenatal diagnosis in every pregnancy in future. A cooperative trend and a positive attitude toward the prevention of beta-thalassemia were noticed in the families with affected children and in the general population.

We present here an analysis of 888 unrelated beta-thal chromosomes consisting of 444 transfusion dependent children from various regions of Punjab and Islamabad Pakistan. By using Multiplex ARMS- PCR, restriction endonuclease analysis, allele specific oligonucleotide (ASO) hybridization and sequencing, 17 beta-thal mutations and 3 Hb variants were detected in 99.5 % (884/888) of the chromosomes analyzed. First trimester prenatal diagnosis by chorionic villus sampling (CVS) was also carried out in seven pregnancies at risk of beta-thalassemia. Our results indicate that three most common mutations accounted for 86.8% of the beta-thal alleles in this region. These findings have important implications for prevention of beta-thalassemia through genetic counseling and prenatal diagnosis in this part of Pakistan.

Objectives: To report a rare case of moyamoya syndrome with primary antiphospholipid syndrome (APS)., Clinical Presentation and Intervention: A 41-year-old woman was admitted with sudden onset of left-sided hemiparesis. Magnetic resonance imaging and magnetic resonance angiography of the brain showed characteristic features of moyamoya vessels. Laboratory investigations revealed raised levels of anticardiolipin antibody. She made a complete and uneventful recovery with aspirin and intensive physiotherapy over a period of 2 weeks., Conclusion: The association of APS and moyamoya disease may present a dilemma in management. While APS is often considered to be an indication for giving anticoagulation, a trial of antiplatelet agents is preferable to anticoagulation because of the risk of bleeding from the fragile moyamoya vessels., (Copyright 2006 S. Karger AG, Basel.)

Ectodermal dysplasia (ED) represents a heterogeneous group of genetic disorders characterized by the absence or deformity in two or more of the ectodermal appendages. We have studied an autosomal recessive form of ED in 13 individuals over six generations from an inbred Pakistani family. The clinical features of the affected individuals include highly dystrophic nails and thin hair on scalp, fine eyebrows and eyelashes, and thin body hair. Genome-wide linkage analysis of 390 microsatellite markers mapped the ED gene to the 3.92 cM interval flanked by markers D10S1710 and D10S1741 on chromosome 10q24.32-q25.1. Multipoint linkage analysis generated a maximum logarithm of odds ratio score of 4.79 in the interval D10S1239-D10S1264, which corresponds to 6.35 Mb.

Objective: To determine the efficacy and safety of transcatheter embolization of nonvariceal gastrointestinal hemorrhage., Design: Descriptive analytical study., Place and Duration of Study: Radiology Department, The Aga Khan University Hospital, Karachi, Pakistan, from October 1999 to August 2004., Subjects and Methods: The study included 27 patients who underwent angiography for suspected gastrointestinal hemorrhage. The presumptive diagnosis was based on the findings of endoscopy in 08 patients, scintigraphy in 05 patients and on clinical findings only in 14 patients. Nine patients had gastroduodenal hemorrhage, 04 patients had hemobilia and 14 patients had lower gastrointestinal bleeding. Five patients had comorbidities. One patient had uremia, 2 had hepatitis C, 1 had cirrhosis and 1 had coagulopathy. Technical success, clinical success, complications and patient survival were assessed., Results: Nine patients had upper gastrointestinal hemorrhage, 4 patients had hemobilia and 14 patients had lower gastrointestinal bleeding. Etiology of bleeding was known in 15 patients and unknown in 12 patients. Clinical success was achieved in 23 (85.18%) of 27 patients. Two patients had early rebleeding. One of these was managed surgically and recovered. The other had severe coagulopathy and was unfit for surgery. Two patients had bowel infarction, one of these was managed surgically and recovered and the other died of complication of procedure. One patient had small splenic infarcts and was managed conservatively. Overall 85.18% success was achieved for the embolization procedure. Two of 27 patients died. One patient died because of recurrence of bleeding and other died because of infarction of right hemi-colon which occurred as complication of embolization., Conclusion: Transcatheter embolization is effective and safe in patients with nonvariceal gastrointestinal hemorrhage.

The traditional catalyst for direct methanol fuel cells (DMFC) is usually platinum, but the high price of noble metals limits the commercialization of DMFC. To develop a non-noble metals catalyst with low cost, simple process and high efficiency for DMFC, the nickel/graphene/polyaniline (NGP) composites were synthesized by one-step electrochemical method under a deposition voltage of 0.5 V for the deposition time ranged from 50 to 250 s. The NGP composite materials was characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). The XRD result showed that the crystal peaks of Ni appeared as NGP composite electrodeposited at 0.5 V for 100 s, and the FTIR result indicated that polyaniline has been successfully prepared by electrodeposition at 0.5 V for 100 s. Large Ni crystals with diameters ranging from 1 to 2 μm, which was embedded in graphene sheets coated by polyaniline, can be observed from SEM. Furthermore, their catalytic performance for the oxidation of methanol in an alkaline medium were investigated. The result showed that the NGP composite material prepared under a deposition voltage of 0.5 V and a deposition time of 200 s exhibited excellent catalytic effects for the methanol oxidation reaction. The peak current density of catalytic methanol oxidation reached 5.01 mA/cm2. According to chronoamperometric analysis, the current density was stable during 1000 s and finally remained 12.5% of the initial current density, it demonstrated the remarkable stability. [ABSTRACT FROM AUTHOR]

Background: Brain metastasis is one of the main causes of recurrence and death in non-small cell lung cancer (NSCLC). Although radiotherapy is the main local therapy for brain metastasis, it is inevitable that some cancer cells become resistant to radiation. Microglia, as macrophages colonized in the brain, play an important role in the tumor microenvironment. Radiotherapy could activate microglia to polarize into both the M1 and M2 phenotypes. Therefore, searching for crosstalk molecules within the microenvironment that can specifically regulate the polarization of microglia is a potential strategy for improving radiation resistance. Methods: We used databases to detect the expression of MIF in NSCLC and its relationship with prognosis. We analyzed the effects of targeted blockade of the MIF/CD74 axis on the polarization and function of microglia during radiotherapy using flow cytometry. The mouse model of brain metastasis was used to assess the effect of targeted blockade of MIF/CD74 axis on the growth of brain metastasis. Result: Our findings reveals that the macrophage migration inhibitory factor (MIF) was highly expressed in NSCLC and is associated with the prognosis of NSCLC. Mechanistically, we demonstrated CD74 inhibition reversed radiation-induced AKT phosphorylation in microglia and promoted the M1 polarization in combination of radiation. Additionally, blocking the MIF-CD74 interaction between NSCLC and microglia promoted microglia M1 polarization. Furthermore, radiation improved tumor hypoxia to decrease HIF-1α dependent MIF secretion by NSCLC. MIF inhibition enhanced radiosensitivity for brain metastasis via synergistically promoting microglia M1 polarization in vivo. Conclusions: Our study revealed that targeting the MIF-CD74 axis promoted microglia M1 polarization and synergized with radiotherapy for brain metastasis in NSCLC. [ABSTRACT FROM AUTHOR]

Amid the rapid evolution of renewable energy, the significance of green hydrogen is growing substantially. Hydrogen is the inevitable future of fuel, where its green production remains a challenge. Photoelectrochemical water splitting (PEC-WS) is the most promising green hydrogen production method utilizing the abundance of both Sun energy and water as the main advantages. However, studies scrutinizing PEC-WS from bibliometric analysis and visual perspectives remain scarce. This research endeavors to illuminate the prevailing landscape of PEC-WS via journal visualization analysis and to foster an enriched comprehension of the ongoing trajectory by means of a comprehensive bibliometric evaluation encompassing Scopus bibliometric databases from diverse relevant journals. Our assessment encompassed a corpus of 2903 articles sourced from 72 reputable journals spanning the years from 1981 to August 22, 2023, encapsulating a 42-year period. Over this span, we meticulously scrutinized various parameters including annual advancements, keyword co-occurrence, global participation, funding sponsors, impactful articles, and recent strides in photoanode materials. The findings reveal the growing interest in research in photoanode materials for PEC-WS since 2011. The analysis also identifies key research trends, notably the increasing focus on earth-abundant materials to achieve high solar-to-hydrogen (STH) efficiency. Additionally, this study sheds light on the growing interest in the integration of artificial intelligence (AI) to expedite the development of PEC-WS photoanodes, marking a pivotal advancement in materials discovery and optimization. In the realm of research output, China plays a prominent dual role as both the most contributive country and a leading funding sponsor. We suggest that these research findings are able to influence educational programs, industry reports, policymakers, and funding initiatives. [ABSTRACT FROM AUTHOR]

A novel hybrid ternary metallic electrocatalyst of amorphous Mo/Co oxides and crystallized Cu metal was deposited over Ni foam using a one-pot, simple, and scalable solvothermal technique. The chemical structure of the prepared ternary electrocatalyst was systematically characterized and confirmed via XRD, FTIR, EDS, and XPS analysis techniques. FESEM images of (Mo/Co)Ox–Cu@NF display the formation of 3D hierarchical structure with a particle size range of 3–5 µm. The developed (Mo/Co)Ox–Cu@NF ternary electrocatalyst exhibits the maximum activity with 188 mV and 410 mV overpotentials at 50 mA cm−2 for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), respectively. Electrochemical impedance spectroscopy (EIS) results for the (Mo/Co)Ox–Cu@NF sample demonstrate the minimum charge transfer resistance (Rct) and maximum constant phase element (CPE) values. A two-electrode cell based on the ternary electrocatalyst just needs a voltage of about 1.86 V at 50 mA cm−2 for overall water splitting (OWS). The electrocatalyst shows satisfactory durability during the OWS for 24 h at 10 mA cm−2 with an increase of only 33 mV in the cell potential. [ABSTRACT FROM AUTHOR]

Carboplatin/pegylated liposomal doxorubicin/bevacizumab is an accepted standard anti-cancer treatment option for recurrent ovarian cancer. However, the occurrence of adverse events associated with this therapeutic regimen limits its continued clinical use. Among these adverse events, acquired amegakaryocytic thrombocytopenia is a rare but often potentially life-threatening adverse effect, and is intolerant to multiple treatment approaches. We report, for the first time, the successful treatment using avatrombopag combined with cyclosporine in one case of carboplatin/pegylated liposomal doxorubicin/bevacizumab-induced acquired amegakaryocytic thrombocytopenia, which was refractory or intolerant to glucocorticoids, intravenous immunoglobulin, recombinant human thrombopoietin, androgen, and even thrombopoietin receptor receptor agonist eltrombopag and herombopag. To date, this case manifests as normal platelet counts that are independent of transfusion. Our findings suggest that this combination is a potential and valuable alternative in acquired amegakaryocytic thrombocytopenia. [ABSTRACT FROM AUTHOR]

The development of nonnoble metal oxygen reduction reaction (ORR) catalysts for fuel cells has been motivated by the high cost and limited supply of noble metals, as well as the desire to improve the performance and durability of this type of energy conversion device. In this study, nonnoble Fe–N–C catalyst was synthesized using a zeolitic imidazole framework (ZIF‐8), poly (aniline), and 10,10′‐dibromo‐9,9′‐bianthry as precursors to produce Fe–N–C with hollow sphere (HS), amorphous bulky structure (B), and sheet‐like thin sheet (N) structure. The Fe–N–C catalyst was analysed in terms of their shape, crystal structure, pore characteristics, and elemental composition. Among all the Fe–N–C catalysts, Fe–N–C_HS had the highest total surface area, followed by Fe–N–C_B and Fe–N–C_N. To evaluate their ORR catalytic activity, a half‐cell electrochemical experiment with.1 M KOH and.1 M HClO4 as the alkaline and acidic electrolytes was conducted. This study revealed that Fe–N–C_HS exhibited the highest onset potential but the Fe–N–C_B has the highest limiting current density in alkaline medium; meanwhile, in acidic media, Fe–N–C_HS shows the best ORR performance with the highest onset potential and limiting current. This highly porous Fe–N–C_HS catalyst also demonstrated active site activation and excellent stability compared with the other samples as well as commercial Pt/C in acidic electrolyte, which suggests its potential for application in proton exchange membrane fuel cells (PEMFCs). [ABSTRACT FROM AUTHOR]

Background: Bartonella henselae is a species of intracellular bacteria transmitted to humans through animal bites and scratches contaminated with the feces of arthropod vectors, and are most commonly associated with cat exposure although transmission from other mammals has been reported. Bartonella henselae infection has a spectrum of clinical manifestations and has rarely been reported as cause of hemophagocytic lymphohistiocytosis (HLH) in immunocompromised hosts. Case presentation: We present a report of Bartonella henselae infection progressing to HLH in an immunocompetent patient. The patient initially presented with regional lymphadenopathy but the diagnosis was not suspected as the patient reported no exposure to cats. On further history, he did report a scratch from a dog prior to development of symptoms. The patient was treated with methylprednisolone, intravenous immunoglobulin and anakinra for the HLH and three months of Doxycycline for Bartonella infection, with complete resolution of symptoms. Conclusions: Although commonly associated with cat exposure, Bartonella henselae transmission can occur after exposure to other animals and vectors including dogs and clinicians need to maintain an index of suspicion for timely diagnosis. Bartonella henselae is associated with a spectrum of clinical manifestations which can include disseminated infection with severe complications such as hemophagocytic lymphohistiocytosis. Prompt initiation of Bartonella treatment is essential when thought to be the trigger for hemophagocytic lymphohistiocytosis although the optimal treatment regimen is unclear. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Coincidences in medicine are not so common. We are presenting a case of a patient diagnosed with Moya‐Moya disease and antiphospholipid syndrome (APS) who presented with clinical and laboratory characteristics of catastrophic APS versus TTP. The diagnosis was a challenge because characteristics were overlapping. Nevertheless, a decision to treat the patient for TTP was made with afterward improvement. MMD has been associated with multiple immune disorders; however, only one case of acquired thrombotic thrombocytopenic purpura has been documented in association with this disease. None has been associated with catastrophic antiphospholipid syndrome. We are presenting a challenging case where all these three medical conditions were present at the same time. [ABSTRACT FROM AUTHOR]

Background: Hereditary hearing loss (HHL) is a common heterogeneous disorder affecting all ages, ethnicities, and genders. The most common form of HHL is autosomal recessive non-syndromic hearing loss (ARNSHL), in which there is no genotype–phenotype correlation in the majority of cases. This study aimed to identify the genetic causes of hearing loss (HL) in a family with Iranian Azeri Turkish ethnicity negative for gap junction beta-2 (GJB2), gap junction beta-6 (GJB6), and mitochondrially encoded 12S rRNA (MT-RNR1) deleterious mutations. Methods: Targeted genome sequencing method was applied to detect genetic causes of HL in the family. Sanger sequencing was employed to verify the segregation of the variant. Finally, we used bioinformatics tools and American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines to determine whether the detected variant might affect the corresponding protein or not. Results: A novel homozygous missense mutation, c.499G>A (p.G167R), was identified in exon 5 of the ESRRB (estrogen-related receptor beta) gene. Healthy and affected family members confirmed the co-segregation of the variant with ARNSHL. Eventually, the variant's pathogenicity was confirmed by the in silico analysis and the ACMG/AMP guidelines. Conclusion: The study suggests that the detected variant, c.499G>A, plays a crucial role in the development of ARNSHL, emphasizing the clinical significance of the ESRRB gene in ARNSHL patients. Additionally, it would be helpful for genetic counseling and clinical management of ARNSHL patients and providing preventive opportunities. [ABSTRACT FROM AUTHOR]

β-Thalassaemia, the most common monogenic disorder, is characterized by genetic heterogeneity at the molecular level. More than 300 mutations of the β globin gene have been characterized all over the world, however, few common mutations account for majority of the cases in various populations. The present study aimed to screen known cases of β-thalassaemia in the Western part of Rajasthan state for five common mutations. The study included 144 known cases of β-thalassaemia of all clinical phenotypes. Cases were diagnosed based on clinical features, haematology investigations including haemogram and Hb-HPLC. Blood samples from cases were taken for mutation analysis. After DNA extraction, mutations were characterized by the polymerase chain reaction method employing allele specific priming technique (AMRS) to study the five mutations including IVS-I-5 (G → C), IVS-I-1 (G → T), CD41/42 (–TCTT), CD 8/9 (+G) and 619 bp deletion from the 3′ end of the β-globin gene using a total of seven different primers. Of all 144 cases, 74 (51.38% of all) cases were of β-thalassaemia major, five (3.4% of all) cases were of β-thalassaemia intermedia and 65 (45.14% of all) cases were of β-thalassaemia minor. Mutation analysis revealed that five common mutations were present in 130 (90.27% of all) cases. Among identified mutations, highest frequency of mutation was of IVS-I-5 (G → C) identified in 73 cases (50.7% of all cases). In 11 (7.63% of all) cases, more than one mutation was identified. β-Thalassaemias are common in Western Rajasthan; however, there is dearth of literature from this part of the country. We observed that five common mutations are common in this part of the country also. These observations are helping us in forming the basis for comprehensive diagnostic database that would not only be useful for genetic counselling but also for prenatal diagnosis. [ABSTRACT FROM AUTHOR]

Cluster‐assembled materials (CAMs) present delicate structures and tailored properties, which have gained extensive interest in recent years. Due to the noncovalent linkage and size of sub‐1 nm, clusters present polymer‐like solution behaviors distinct from conventional nanocrystals, whereby a variety of CAMs with different constructions have been fabricated. To our knowledge, the morphologies and properties of CAMs are closely connected, which are strongly dependent on the structure and coordination state of cluster building blocks. For the CAMs with superior performances, the specific arrangement and surroundings of clusters at subnanometer scale usually result in well‐organized structures. In this review, we will summarize the recent progress of CAMs with highly ordered structures and advanced properties, where polyoxometalate cluster assemblies as well as cluster–nuclei co‐assemblies are involved. The application potentials of CAMs are then discussed, especially on the cluster arrangements and interactions at subnanometer scale. However, current researches are far from reaching the point, and future efforts are needed before the nature of cluster‐based nanostructures can be sufficiently revealed. We hope this article can offer unique insights and instructive understanding to the CAMs. [ABSTRACT FROM AUTHOR]

Zirconium (1,3,5,2λ5,4λ5,6λ5-triazatriphosphinine-2,2,4,4,6,6-hexyl) hexa(phosphate) (ZrTHPA) is synthesized by chelation combination (1,3,5,2λ5,4λ5,6λ5-triazatriphosphinine-2,2,4,4,6,6-hexyl) hexa(phosphonic acid) (THPA) with zirconium ion in a certain ratio. The THPA is synthesized by hexachlorocyclotriphosphazene with P(OEt)3, and hydrolyzed in HCl. Their structure is characterized by nuclear magnetic resonance (NMR) and infrared spectroscopy (IR), and hydrogen bonding and chelation interactions between Zr4+ and phosphonic acid are detected. X-ray diffraction and scanning electron microscopy (SEM) show that ZrTHPA has a layered structure. Thermogravimetry-differential thermal analysis (TG-DTA) shows that ZrTHPA has good thermal stability. ZrTHPA also has good oxidative stability and hydrolysis resistance. The ion exchange capacity of ZrTHPA (1:2) is 2.21 meq g−1. At 180 °C, the proton conductivity of ZrTHPA (1:2) at 100%, 50%, and 0 relative humidity is 0.128 S cm−1, 0.069 S cm−1, and 0.038 S cm−1, respectively. ZrTHPA can be used as solid acid catalyst or proton conductor which is doped into proton exchange membranes. [ABSTRACT FROM AUTHOR]

Thalassemia,is one of the most important hemolytic genetic, diseases that break down red blood cells, Children with thalassemia cannot produce. enough hemoglobin because; the bone marrow cannot produce enough red blood cells to meet their needs and the red blood cells produced,by them are almost devoid of hemoglobin, and the disease is divided into alpha, and Beta thalassemia. The current study was designed with the aim of .investigating the relationship between serum ferritin and hepatic enzymes in thalassemia. patients, iron is stored primarily in the form of,ferritin in liver cells. The study included 60 patients with thalassemia in; Dhi Qar governorate, distributed between. the two gender during the, research period, their ages ranged between (2 -26) years old, blood samples were taken in order to testing of. hemoglobin, ferritin, and liver enzymes (ATP, GOT, GPT), and the same, tests were performed on healthy people 40 person ;as they are a control group. The results recorded; a high percentage of,thalassemia incidence in males and a decrease in females. The study also showed; that the incidence of thalassemia reached its peak in the age. group (2-6) years and decreased with age, as it reached its lowest levels in the age, group (22-26) years. The study revealed .a significant decrease (0.05> P) in hemoglobin concentration in thalassemia, patients compared with the ;control group, and the results recorded a significant; increase (0.05 P <) in the concentration of ferritin in thalassemia, patients compared with the control group. The current study; also showed a significant increase (P <0.05) in GPT enzyme concentration in thalassemia. patients compared with the control group. There was a significant increase (P <0.05) of GOT enzyme; concentration in patients compared to the,control group. The results indicated. that there was a significant increase (P <0.05) in the concentration of the ALP enzyme in the patients. compared with the control group. [ABSTRACT FROM AUTHOR]

Cotton is a premium source of natural fiber and is considered “white gold” in the textile industry. Fiber length, strength and fineness are the key considerations for the industry. Longer fibers are machine friendly because they are easily spinnable. Recent advancements in genetic engineering, including the development of DNA markers and quantitative trait loci (QTLs), together with genome sequencing and gene expression profiling, have provided new avenues for improving fiber production and quality. In plants, sucrose synthase (SUS) is the key enzyme that catalyzes the reversible cleavage of sucrose and uridine diphosphate (UDP) into fructose and UDP-glucose. Sucrose is the main mobile sugar in plants moving from source to sink. It regulates resource partitioning between active sinks, especially in cotton embryos and fibers, and therefore is directly involved in determining fiber yield and seed quality. SUS actively takes part in regulating the competition for nutrients among sink tissues through balancing osmotic potentials by providing hexoses and an efficient supply of UDP-glucose the substrate for cellulose synthase. Cotton transformation has been used to improve fiber characteristics by altering cell wall properties through the manipulation of expression of fiber genes. Overexpression of the SUS gene from natural or synthetic origins in cotton can be an excellent way to solve potential problems associated with poor fiber length and other fiber quality traits. Increased SUS activity can result in more hexoses, increasing the osmotic potential and thereby driving the water influx that creates high turgor pressure in fiber cells resulting in enhanced fiber elongation. Moreover, increased SUS gene transcript levels in vegetative tissues of the plant will elevate seedling biomass and seed number. Fiber length and seed number both contribute towards final yield and the SUS genes as key regulators of sink strength in cotton perform this dual function that is directly related to cotton productivity. Hence manipulation of the SUS gene family is considered a promising approach to improve cotton fiber yield and quality. This review focuses on the biochemical and physiological roles of the SUS genes and there value for cotton fiber improvement. [ABSTRACT FROM AUTHOR]

Background: Pure hair and nail ectodermal dysplasia (PHNED) is a congenital disorder of hair abnormalities and nail dysplasia. Both autosomal recessive and dominant inheritance fashion of PHNED occurs. In literature, to date, five different forms of PHNED have been reported at molecular level, having three genes known and two loci with no gene yet. Methods: In this study, a four generations consanguineous family of Pakistani origin with autosomal recessive PHNED was investigated. Affected members exhibited PHNED phenotypes with involvement of complete hair loss and nail dysplasia. To screen for mutation in the genes (HOXC13, KRT74, KRT85), its coding exons and exons-intron boundaries were sequenced. The 3D models of normal and mutated HOXC13 were predicted by using homology modeling. Results: Through investigating the family to known loci, the family was mapped to ectodermal dysplasia 9 (ECTD9) loci with genetic address of 12q13.13. Mutation screening revealed a novel missense mutation (c.929A > C; p. Asn310Thr) in homeobox DNA binding domain of HOXC13 gene in affected members of the family. Due to mutation, loss of hydrogen bonding and difference in potential energy occurs, which may resulting in alteration of protein function. Conclusion: This is the first mutation reported in homeodomain, while 5th mutation reported in HOXC13 gene causing PHNED. [ABSTRACT FROM AUTHOR]

Pure hair and nail ectodermal dysplasia ( PHNED) is a rare disorder that presents with hypotrichosis and nail dystrophy while sparing other ectodermal structures such as teeth and sweat glands. We describe a homozygous novel missense mutation in the HOXC13 gene that resulted in autosomal recessive PHNED in a Hispanic child. The mutation c.812A>G (p.Gln271Arg) is located within the DNA-binding domain of the HOXC13 gene, cosegregates within the family, and is predicted to be maximally damaging. This is the first reported case of a missense HOXC13 mutation resulting in PHNED and the first reported case of PHNED identified in a North American family. Our findings illustrate the critical role of HOXC13 in human hair and nail development. [ABSTRACT FROM AUTHOR]

The purpose of this research is to investigate the role of information & communication technology (ICT) and e-governance in health sector of Pakistan, where these facilities are at initial stage of development. This study utilizes primary data collected from 170 patients in both public and private hospitals of district Peshawar Pakistan. This is an exploratory study and descriptive statistics are being used for the analysis of the data. The result of the study shows that the use of e-governance in health sector is at a rudimentary stage in the study area. Few people use ICT for medical purposes but the overall level is low. There is need of awareness. In addition, the facilitating conditions for the adoption of e-governance in health sector include infrastructure, electricity, proper user interface and data privacy & confidentiality. The government needs to initiate practical steps for the implementation of e-governance in health sector. This will improve the efficiency, transparency, and accountability of the system. Moreover, it will also speed up the service delivery system. The study is valuable as it explored the role of ICT and e-governance in health sector of Pakistan for the first time. Therefore, this study contributed to literature in a positive way. [ABSTRACT FROM AUTHOR]