27 results on '"Tongtong Ren"'
Search Results
2. Xuebijing Injection Maintains GRP78 Expression to Prevent Candida albicans–Induced Epithelial Death in the Kidney
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Ting Shang, Qilin Yu, Tongtong Ren, Xin-Tong Wang, Hongyan Zhu, Jia-Ming Gao, Guixiang Pan, Xiumei Gao, Yan Zhu, Yuxin Feng, and Ming-Chun Li
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fungal infection ,C. albicans ,Xuebijing injection ,endoplasmic reticulum stress ,GRP78 ,Chinese medicine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Sepsis and septic shock threaten the survival of millions of patients in the intensive care unit. Secondary fungal infections significantly increased the risk of mortality in sepsis patients. Chinese medicine Xuebijing injection (XBJ) has been routinely used as an add-on treatment to sepsis and septic shock in China. Our network pharmacology analysis predicted that XBJ also influences fungal infection, consisting with results of pioneer clinical studies. We conducted in vivo and in vitro experiments to verify this prediction. To our surprise, XBJ rescued mice from lethal Candida sepsis in a disseminated Candida albicans infection model and abolished the colonization of C. albicans in kidneys. Although XBJ did not inhibit the growth and the virulence of C. albicans in vitro, it enhanced the viability of 293T cells upon C. albicans insults. Further RNA-seq analysis revealed that XBJ activated the endoplasmic reticulum (ER) stress pathway upon C. albicans infection. Western blot confirmed that XBJ maintained the expression of GRP78 in the presence of C. albicans. Interestingly, key active ingredients in XBJ (C0127) mirrored the effects of XBJ. C0127 not only rescued mice from lethal Candida sepsis and prevented the colonization of C. albicans in kidneys, but also sustained the survival of kidney epithelial cells partially by maintaining the expression of GRP78. These results suggested that XBJ may prevent fungal infection in sepsis patients. Pre-activation of ER stress pathway is a novel strategy to control C. albicans infection. Network pharmacology may accelerate drug development in the field of infectious diseases.
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- 2020
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3. F̄-ladder knowledge and F̄-hiding knowledge discovery.
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Xiumei Hao, Tongtong Ren, and Yanli Zhang
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- 2015
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4. Performance improvement potential of integrated process of urea‐adduction and distillation for separation of 1‐octene from <scp>F–T</scp> synthetic products
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Tongtong Ren, Suli Liu, Hong Li, Jiuzhou Chen, Xingang Li, Angui Zhang, and Xin Gao
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Inorganic Chemistry ,Fuel Technology ,Renewable Energy, Sustainability and the Environment ,General Chemical Engineering ,Organic Chemistry ,Pollution ,Waste Management and Disposal ,Biotechnology - Published
- 2022
5. Research on the Integration of China's Tourism Industry and Financial Industry under the Background of 'the Belt and Road'
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Weiling Su and Tongtong Ren
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Environmental sciences ,GE1-350 - Abstract
Under the background of "the Belt and Road", the rapid development of tourism and financial industry in China has made important contributions to the economic and cultural construction of "the Belt and Road ". Through the construction of coupling correlation model and coupling index system, this paper conducts an empirical analysis on the integrated development of tourism and financial industry in China from 2013 to 2017 under the background of "the Belt and Road". The results show that China's tourism and financial industry have developed rapidly in the period of 2013-2017, and there is a significant coupling relationship between them. However, there is a lag in the development of financial industry in 2013-2014 and a lag in the development of tourism industry in 2015-2017. In general, under the background of "the Belt and Road", China's tourism and financial industry continue to develop, and the links and interactions between the two industries are getting stronger and stronger. The two industries promote each other, and finally achieve the high-quality coordinated development of tourism and financial industry.
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- 2021
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6. scWECTA: A weighted ensemble classification framework for cell type assignment based on single cell transcriptome
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Tongtong Ren, Shan Huang, Qiaoming Liu, and Guohua Wang
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Health Informatics ,Computer Science Applications - Abstract
Rapid advances in single-cell transcriptome analysis provide deeper insights into the study of tissue heterogeneity at the cellular level. Unsupervised clustering can identify potential cell populations in single-cell RNA-sequencing (scRNA-seq) data, but fail to further determine the identity of each cell. Existing automatic annotation methods using scRNA-seq data based on machine learning mainly use single feature set and single classifier. In view of this, we propose a Weighted Ensemble classification framework for Cell Type Annotation, named scWECTA, which improves the accuracy of cell type identification. scWECTA uses five informative gene sets and integrates five classifiers based on soft weighted ensemble framework. And the ensemble weights are inferred through the constrained non-negative least squares. Validated on multiple pairs of scRNA-seq datasets, scWECTA is able to accurately annotate scRNA-seq data across platforms and across tissues, especially for imbalanced data containing rare cell types. Moreover, scWECTA outperforms other comparable methods in balancing the prediction accuracy of common cell types and the unassigned rate of non-common cell types at the same time. The source code of scWECTA is freely available at https://github.com/ttren-sc/scWECTA.
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- 2022
7. A Review of the Developed New Model Biodiesels and Their Effects on Engine Combustion and Emissions
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Shenghua Liu, Wei Chen, Zengqiang Zhu, Sa Jiang, Tongtong Ren, and Hejun Guo
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biodiesel ,ethylene glycol monomethyl ether ,engine ,combustion ,emission ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Biodiesel is regarded to be a renewable, CO2 neutral and thus sustainable biological alternative diesel fuel. With attention to the reduction of petroleum import, PM 2.5 aerosol particles and the greenhouse effect gas CO2, biodiesel has drawn great research interests and efforts in the past decade in China. Generally, biodiesel refers to fatty acid methyl ether (FAME) which has a proved effect in reducing diesel emission, particularly PM. However, FAME has a limited cetane number and oxygen content, to study the effects of elevated cetane number and oxygen content on fuel properties, engine combustion and emissions, ethylene glycol monomethyl ether is used to produce a series of new models of biodiesels by transesterification method. The feedstocks are rapeseed oil, soybean oil, peanut oil, palm oil and cottonseed oil. Ether group alcohols used in this study include ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, propylene glycol monomethyl ether, diethylene glycol monomethyl ether, triethylene glycol monomethyl ether. The molecular structure was proved by FT-IR and NMR analyses. Fuel properties were measured based on the corresponding standards. The developed new model biodiesels have cetane number (CN) over 70 and oxygen content over 17% by mass, which are higher than FAME (50 CN and 11% oxygen). They have the same level of lower heating value as FAME, but have a higher density, which helps to compensate the decrease of engine power. Meanwhile, the engine tests were carried out to investigate the effects of ether ester group on engine combustion and emissions. The test results show that FAME reduced smoke 30% to 50%, while the new model biodiesel fuels reduced engine smoke as high as 80% and have the potential to decrease engine HC, CO and NOx emissions 50% or more.
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- 2018
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8. Synthesis of antimicrobial peptide-grafted graphene oxide nanosheets with high antimicrobial efficacy
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Qilin Yu, Yufan Wang, Mingchun Li, and Tongtong Ren
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Materials science ,Antimicrobial peptides ,Peptide ,02 engineering and technology ,010402 general chemistry ,medicine.disease_cause ,01 natural sciences ,Microbiology ,law.invention ,chemistry.chemical_compound ,In vivo ,law ,medicine ,General Materials Science ,Candida albicans ,Escherichia coli ,chemistry.chemical_classification ,biology ,Graphene ,Mechanical Engineering ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Antimicrobial ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,Growth inhibition ,0210 nano-technology - Abstract
Graphene nanomaterials have rapidly developed in recent years. However, its antimicrobial activity does not achieve satisfactory effect. During the past years, it has been found that antimicrobial peptides (AMP) have a good inhibitory effect on pathogenic microbes. In this study, we synthesized AMP-grafted graphene oxide (GO) nanosheets, i.e., D28@GO, against the pathogens such as Candida albicans and Escherichia coli. Growth inhibition assays showed that D28@GO had strong inhibitory effect against the pathogens and this toxicity contributes to cell membrane damage caused by interaction between D28@GO and macrobiomolecules. In vivo experiments further showed that D28@GO severely suppressed systemic infections of pathogens. This study provides novel light on development of GO-based antimicrobial materials.
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- 2019
9. Reactive distillation of tertiary olefin etherification for the purification of C8 α-olefin from F-T synthetic products
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Xingang Li, Suli Liu, Angui Zhang, Tongtong Ren, Hong Li, Wei Yuan, and Xin Gao
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Activity coefficient ,Olefin fiber ,Materials science ,Kinetics ,Filtration and Separation ,Analytical Chemistry ,Catalysis ,chemistry.chemical_compound ,chemistry ,Scientific method ,Reactive distillation ,Organic chemistry ,Methanol ,Ion-exchange resin - Abstract
Utilization of F-T synthetic to produce value-added C8 linear α-olefin is a promising alternative approach, however, the purification of α-olefin from F-T synthetic products is a great challenge, especially for removing 2-methyl-1-heptene (MH). This article addresses the novel method of etherification reaction to remove MH from F-T synthetic products, and develop by far the most efficiency reactive distillation (RD) method to intensified the etherification process. The scarcity of thermodynamic data was measured and regressed by the activity coefficient models, furthermore, kinetics of the etherification reaction of MH with methanol was explored using an ion exchange resin as catalyst, and then fitted by a pseudo-homogeneous model. The pilot-scale RD experimental results show that the conversion of MH reaches 91.83 % and the purity of α-olefin increases by 14.9 wt% (percentage points). Process analysis method was used to realize the optimization of design and operating parameters of etherification RD column based on the developed rigorous model. The optimization results show that the conversion of MH achieves 99.74 % and the purity of α-olefin achieves 96.87 wt%, which is also illustrated that the novel method for removing MH is proposed paving the way for purification of α-olefin from F-T synthetic products.
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- 2022
10. Co3O4 nanoparticles at sublethal concentrations inhibit cell growth by impairing mitochondrial function
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Mingchun Li, Nali Zhu, Tongtong Ren, Honggang Wang, and Qilin Yu
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inorganic chemicals ,0301 basic medicine ,Membrane potential ,Programmed cell death ,Chemistry ,Cell growth ,technology, industry, and agriculture ,Biophysics ,Cell Biology ,Mitochondrion ,Matrix metalloproteinase ,Biochemistry ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,Downregulation and upregulation ,Apoptosis ,Toxicity ,Molecular Biology - Abstract
Co3O4 nanoparticles (NPs) are one kind of the important nanomaterials that have the application potential in catalyst, electrochromic devices, sensors, etc. However, their biological effect remains to be detailed. In this study, we investigated the effect of the as-synthesized Co3O4 NPs (15–30 nm) on the growth of mammalian cells, and found that the NPs severely inhibited cell growth at the sublethal concentrations from 12.5 to 200 mg/L. Interestingly, the NPs did not cause obvious cell death and ROS accumulation, indicating that their inhibitory effect was not attributed to both apoptosis- or necrosis-related cell death and ROS accumulation. Transcription profiling analysis revealed that the NPs caused remarkable down regulation of the genes involved in mitochondrial functions. Transmission electron microscopy (TEM) and biochemical analysis further showed that the NPs might interact with the mitochondria, impairing the mitochondrial membrane potential (MMP) and ATP production. This study uncovers a mitochondrial respiratory chain-related and ROS-independent toxicity mechanism of Co3O4 NPs in eukaryotic cells.
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- 2018
11. A turbulent two-phase model for predicting cavitating flow based on homogenous nucleation theory
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Shenghua Liu, Li Donghua, Yanju Wei, Yonghong Tang, and Tongtong Ren
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Materials science ,Turbulence ,General Chemical Engineering ,Bubble ,Nozzle ,Nucleation ,Injector ,Mechanics ,010402 general chemistry ,Condensed Matter Physics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,010305 fluids & plasmas ,0104 chemical sciences ,law.invention ,Vortex ,Physics::Fluid Dynamics ,law ,Cavitation ,0103 physical sciences ,Classical nucleation theory - Abstract
Cavitation is important for good design and performance on engineering machinery, especially for devices such as injector nozzles. In this study, an enhanced cavitation model is proposed with the consideration of vapor transport, bubble dynamics, nucleation, turbulence and the dissolved gas. Based on the classical nucleation theory (CNT), the analysis of nucleation formation process has been carried out in this model. The bubble size distribution obtained from experimental data is also taken into account in this model. Besides, the turbulence effect on the pressure fluctuations is discussed based on the Boussinesq approximation. According to Henry's law, the dissolved gas effect is incorporated into this model by dividing dissolved gas into several different amount and types of gases. All the coefficients used in this model can be either set at recommended values or obtained from previous experiments. The validation of this model is conducted on the experiments of sharp-edged orifice and the NASA CR-2156 hydrofoil. The results show that the proposed model is in good agreement with experiments. This model is also extended into a vortex form in order to simulate the string cavitation phenomenon observed in the visualized multi-orifice diesel nozzle experiment. The proposed model has captured the string cavitation phenomenon and made a preliminary investigation on vortex cavitation inside diesel nozzle.
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- 2018
12. Candida albicans infection disturbs the redox homeostasis system and induces reactive oxygen species accumulation for epithelial cell death
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Lei Tian, Qilin Yu, Hangqi Zhu, Mingchun Li, and Tongtong Ren
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Programmed cell death ,Applied Microbiology and Biotechnology ,Microbiology ,Antioxidants ,Superoxide dismutase ,chemistry.chemical_compound ,Candida albicans ,Homeostasis ,Humans ,chemistry.chemical_classification ,Reactive oxygen species ,Cell Death ,biology ,Epithelial Cells ,General Medicine ,biology.organism_classification ,Glutathione ,Corpus albicans ,HEK293 Cells ,chemistry ,Catalase ,biology.protein ,Reactive Oxygen Species ,Oxidation-Reduction ,Nicotinamide adenine dinucleotide phosphate ,Intracellular - Abstract
Candida albicans is a common pathogenic fungus with high mortality in immunocompromised patients. However, the mechanism by which C. albicans invades host epithelial cells and causes serious tissue damage remains to be further investigated. In this study, we established the C. albicans–293T renal epithelial cell interaction model to investigate the mechanism of epithelial infection by this pathogen. It was found that C. albicans infection causes severe cell death and reactive oxygen species (ROS) accumulation in epithelial cells. Further investigations revealed that C. albicans infection might up-regulate expression of nicotinamide adenine dinucleotide phosphate (NAPDH) oxidase (NOX), inhibit the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), and suppress the p38–Nrf2–heme oxygenase-1 (HO-1) pathway which plays an important role in the elimination of intracellular ROS. Furthermore, epithelial cell death caused by the fungal infection could be strikingly alleviated by addition of the antioxidant agent glutathione, indicating the critical role of ROS accumulation in cell death caused by the fungus. This study revealed that disturbance of the redox homeostasis system and ROS accumulation in epithelial cells is involved in cell death caused by C. albicans infection, which sheds light on the application of antioxidants in the suppression of tissue damage caused by fungal infection.
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- 2019
13. The TRP Ca
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Liping, Peng, Qilin, Yu, Henan, Wei, Nali, Zhu, Tongtong, Ren, Chao, Liang, Jiachun, Xu, Lei, Tian, and Mingchun, Li
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Fungal Proteins ,Transient Receptor Potential Channels ,Candida albicans ,Hyphae ,Cell Polarity ,NADPH Oxidases ,Calcium Channels ,Reactive Oxygen Species ,Gene Deletion - Abstract
Candida albicans is an important opportunistic fungal pathogen, and hyphal polarized growth is critical for its invasive infection to the host. Both the vacuolar transient receptor potential (TRP) Ca
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- 2019
14. Transcription profiling-guided remodeling of sulfur metabolism in synthetic bacteria for efficiently capturing heavy metals
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Youjun Zhang, Rongguang Shi, Qilin Yu, Jinpeng Liu, Nali Zhu, Meiting Ju, Tongtong Ren, Ting Ma, Xiaohua Li, and Chaofeng Shao
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Environmental Engineering ,Operon ,Health, Toxicology and Mutagenesis ,0211 other engineering and technologies ,Sulfur metabolism ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Metal ,Transcription (biology) ,Metals, Heavy ,medicine ,Humans ,Environmental Chemistry ,Waste Management and Disposal ,Escherichia coli ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,biology ,Chemistry ,Cupriavidus taiwanensis ,Cupriavidus ,biology.organism_classification ,Pollution ,Sulfur ,Biochemistry ,visual_art ,visual_art.visual_art_medium ,Adsorption ,Bacteria - Abstract
Heavy metal contamination is becoming a global problem threatening human health. Heavy metal removal by engineered microbes by cellular adsorption and uptake is a promising strategy for treatment of heavy metal contamination. However, this strategy is confronted with limited heavy metal-capturing elements. In this study, we performed a transcription profiling-guided strategy for construction of heavy metal-capturing synthetic bacteria. Transcription profiling of a heavy metal-tolerating Cupriavidus taiwanensis strain revealed up-regulation of sulfur metabolism-related operons (e.g., iscSAU and moaEDAB) by Pb2+ and Cd2+. A synthetic Escherichia coli strain, EcSSMO, was constructed by design of a synthetic sulfur metabolism operon (SSMO) based on iscSAU/moaEDAB. Biochemical analysis and X-ray photoelectron spectroscopy (XPS) revealed that the synthetic bacteria had remodeled sulfur metabolism and enhanced heavy metal-tolerating capacity, with higher surviving EcSSMO cells than the surviving control cells Ec0 (not containing SSMO) at 50 mg/L of Pb2+ and Cd2+ (>92 % versus 90 % of Pb2+ and Cd2+ at 5 mg/L of Pb2+ and Cd2+, and >40 % of both heavy metals even at 50 mg/L of Pb2+ and Cd2+. This study reveals emphasizes feasibility of transcription profiling-guided construction of synthetic organisms by large-scale remodeling metabolic network.
- Published
- 2021
15. Analysis on the Effects of Morphological Traits of Lutraria sieboldii on Its Body Mass Trait.
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Dan GUO, Jian CHEN, Ziqin ZHAI, Tongtong REN, Jijin WANG, Lirong BAI, and Dahui YU
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MULTIVARIATE analysis ,SHELLFISH ,REGRESSION analysis ,PATH analysis (Statistics) ,STATISTICAL correlation ,MULTIPLE regression analysis - Abstract
[Objectives] This study was conducted to explore the relationship between morphological traits and body mass trait of Lutraria sieboldii. [Methods] 110 were randomly selected from 120 2n,i-instar L. sieboldii collected from the Tieshangang area of Beihai, Guangxi, and 132 were randomly selected from 150 shellfish at the instar of 0.6. Their morphological traits were measured: shell length (SL), shell height (SH), shell width (SW), anterior length (AL), posterior length (PL), nose length (NL) in closed shell state, and maximum open shell width (OS) between two shells in closed shell state, and the body mass trait BM was also measured. Statistical methods such as path analysis and multiple regression were used for data analysis, and the effects of these seven morphological traits on the body mass trait were studied, respectively. The correlation between the tested seven quantitative traits and one body mass trait was all positive, all reaching an extremely significant level (P <0.01). [Results] The body mass trait of the shellfish at the instar of 2 had the highest correlation coefficient with shell length (0. 922), that is, shell length had the greatest direct impact on the body mass trait; the path coefficient was 0.700; and the final multiple regression equation established was BM - -124.882 + 1.189SL + 1.551 SH+1.035SW + 0.119NL, and the total determination coefficient (ß2) on body mass was 0.849. The body mass trait of the shellfish at the instar of 0. 6 had the highest correlation coefficient with shell length (0. 859), that is, shell length had the greatest direct impact on the body mass trait; the path coefficient was 0.494; and the final multiple regression equation established was BM = -1.917 + 0.111SL +0.021 NL +0.078SW + 0.0320S, and the total determination coefficient (ß2) on body mass was 0. 828. The multivariate regression variance analysis showed that the regression between the morphological traits and body mass trait of the L. sieboldii at the instars of 2 and 0. 6 reached an extremely significant level (P <0. 01). [Conclusions] This study provides a scientific basis for the selection of broodstock in the selection and breeding of L. sieboldii. [ABSTRACT FROM AUTHOR]
- Published
- 2021
16. Chemical preparation, biological evaluation and 3D-QSAR of ethoxysulfuron derivatives as novel antifungal agents targeting acetohydroxyacid synthase
- Author
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Li Wang, Cong-Wei Niu, Jian-Guo Wang, Guo-Qing Song, Mingchun Li, Fuhang Song, Tongtong Ren, Qilin Yu, Wei-Bin Ruan, Lixin Zhang, Jieyu Gao, Ren-Jun Wu, and Zheng Yao
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Quantitative structure–activity relationship ,Antifungal Agents ,Nematoda ,medicine.drug_class ,Quantitative Structure-Activity Relationship ,Gene mutation ,01 natural sciences ,03 medical and health sciences ,Minimum inhibitory concentration ,Mice ,Drug Discovery ,Candida albicans ,medicine ,Animals ,Enzyme Inhibitors ,030304 developmental biology ,Pharmacology ,chemistry.chemical_classification ,0303 health sciences ,biology ,010405 organic chemistry ,Chemistry ,Herbicides ,Organic Chemistry ,General Medicine ,biology.organism_classification ,Sulfonylurea ,0104 chemical sciences ,Amino acid ,Molecular Docking Simulation ,Acetolactate Synthase ,Enzyme ,Sulfonylurea Compounds ,Biochemistry ,Biological target - Abstract
Accetohydroxyacid synthase (AHAS) is the first enzyme involved in the biosynthetic pathway of branched-chain amino acids. Earlier gene mutation of Candida albicans in a mouse model suggested that this enzyme is a promising target of antifungals. Recent studies have demonstrated that some commercial AHAS-inhibiting sulfonylurea herbicides exerted desirable antifungal activity. In this study, we have designed and synthesized 68 novel ethoxysulfulron (ES) derivatives and evaluated their inhibition constants (Ki) against C. albicans AHAS and cell based minimum inhibitory concentration (MIC) values. The target compounds 5-1, 5-10, 5-22, 5-31 and 5-37 displayed stronger AHAS inhibitions than ES did. Compound 5-1 had the best Ki of 6.7 nM against fungal AHAS and MIC values of 2.5 mg/L against Candida albicans and Candica parapsilosis after 72 h. A suitable nematode model was established here and the antifungal activity of 5-1 was further evaluated in vivo. A possible binding mode was simulated via molecular docking and a comparative field analysis (CoMFA) model was constructed to understand the structure-activity relationship. The current study has indicated that some ES derivatives should be considered as promising hits to develop antifungal drugs with novel biological target.
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- 2018
17. Co
- Author
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Honggang, Wang, Tongtong, Ren, Nali, Zhu, Qilin, Yu, and Mingchun, Li
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Mammals ,Membrane Potential, Mitochondrial ,Adenosine Triphosphate ,Gene Expression Profiling ,Animals ,Down-Regulation ,Nanoparticles ,Oxides ,Cobalt ,Cell Line ,Cell Proliferation ,Mitochondria - Abstract
Co
- Published
- 2018
18. The TRP Ca2+ channel Yvc1 regulates hyphal reactive oxygen species gradient for maintenance of polarized growth in Candida albicans
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Qilin Yu, Liping Peng, Henan Wei, Mingchun Li, Chao Liang, Tongtong Ren, Lei Tian, Jiachun Xu, and Nali Zhu
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Hyphal growth ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,NADPH oxidase ,030306 microbiology ,Hyphal tip ,Depolarization ,Biology ,biology.organism_classification ,Microbiology ,Cell biology ,03 medical and health sciences ,EGTA ,chemistry.chemical_compound ,Transient receptor potential channel ,chemistry ,Genetics ,biology.protein ,Candida albicans ,030304 developmental biology - Abstract
Candida albicans is an important opportunistic fungal pathogen, and hyphal polarized growth is critical for its invasive infection to the host. Both the vacuolar transient receptor potential (TRP) Ca2+ channel Yvc1 and the NADPH oxidase Fre8-governed reactive oxygen species (ROS) gradient are involved in hyphal development, but the relationship between Yvc1 and Fre8 during hyphal polarized growth remains to be investigated. Herein, we found that deletion of YVC1 led to dispersed distribution of ROS along the germ tube, while it was concentrated at the hyphal tip in WT cells. Moreover, Fre8 localization was altered as YVC1 was disrupted. Besides, similar to deletion of YVC1, addition of the Ca2+ chelating agent EGTA caused depolarization of Fre8-GFP in the wild-type cells, indicating the critical role of Yvc1-maintained Ca2+ gradient in polarized distribution of Fre8-GFP and consequent disruption of tip ROS gradient. By constructing a series of GFP-tagged polarized growth-related proteins, including Bud6, Exo70 and Lifeact, we found that these proteins, similar to Fre8 and ROS, had depolarized localization in yvc1Δ/Δ. Thus, our work provides a mechanic explanation of Yvc1-governed and ROS-related hyphal polarized growth, and shed a novel light on the role of Ca2+ signaling in maintenance of redox homeostasis and morphogenesis in the fungal pathogens.
- Published
- 2019
19. F̄-ladder knowledge and F̄-hiding knowledge discovery
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Tongtong Ren, Yanli Zhang, and Xiumei Hao
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Discrete mathematics ,Theoretical computer science ,Knowledge extraction ,Degree (graph theory) ,Rough set theory data mining ,Condensed Matter::Strongly Correlated Electrons ,Rough set ,Hardware_ARITHMETICANDLOGICSTRUCTURES ,Dual (category theory) ,Mathematics - Abstract
By employing dual of one direction S-rough sets (dual of one direction singular rough sets) and its dynamic characteristic, this paper presents the concepts of F-ladder knowledge and F-ladder degree. Using these concepts, the discernibility theorem of F-ladder knowledge, the hiding-discovery theorem of maximum F-ladder knowledge, the hiding-discovery theorem of maximum F-ladder knowledge and the dependence-filter theorem of knowledge discovery are proposed, and the interior hiding principle of F-ladder knowledge, the hiding-discovery criterion of F-ladder knowledge and its applications are given. The results given in this paper show the new characteristics of dual of one direction S-rough sets and the new applications of the dynamic characteristic of dual of one direction S-rough sets.
- Published
- 2015
20. Design and Realization of Architecture for Launch Vehicle Test and Simulation System.
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Tongtong Ren, Hui Li, Kun Zhang, and Chun Yang
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COMPUTER architecture ,COMPUTER simulation ,INDUSTRIAL management ,INDUSTRIAL productivity ,COMPUTER security - Abstract
Adopting the Space launch vehicle virtual test platform as research object, and focusing on the key functional demand of distributed virtual test based on numerical simulation, a new architecture based on the Model Driven Architecture (MDA) and High Level Architecture (HLA) is discussed. In the vertical, it separates the federate members' logic behavior and code integrated manner, so that the work of developing the platformindependent model and platform specific model can be finished at the same time. In the horizontal, the using of HLA can partially achieve the reusability of the models. So the benefit using MDA and HLA in simulation is the reusability and interoperability of the models in whole lifecycle of the software. Using the Measurement Studio to develop the virtual instruments can improve the real-time performance and the architecture based on the MDA and HLA can meet the demands of the Launch Vehicle Test and Simulation System. [ABSTRACT FROM AUTHOR]
- Published
- 2009
21. Performance improvement potential of integrated process of urea‐adduction and distillation for separation of 1‐octene from F–T synthetic products.
- Author
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Ren, Tongtong, Liu, Suli, Li, Hong, Chen, Jiuzhou, Li, Xingang, Zhang, Angui, and Gao, Xin
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SYNTHETIC products ,DISTILLATION ,UREA ,PRODUCT recovery ,ENERGY consumption ,CONSUMPTION (Economics) ,PRESSURE swing adsorption process - Abstract
BACKGROUND: C8 α‐olefins are regarded as top value‐added compounds from Fischer–Tropsch (F−T) products. However, the separation of purified C8 α‐olefins has proven to be a daunting challenge owing to their relative volatility. As the traditional methods for C8 α‐olefins purification are energy‐ and capital cost‐intensive, an efficient method for the separation of n‐hydrocarbons and iso‐hydrocarbons will provide a valuable and more sustainable alternative to address these challenges. RESULTS: In the urea‐adduction method, the purity of 1‐octene was increased from 96.02 to 98.04 wt%, and the yield was 93.62 %, which broke the limitation of traditional distillation on the purification of 1‐octene. By combining the urea‐adduction method and distillation, a promising and energy‐efficient integrated separation process was proposed. First, it recovers C8n‐hydrocarbons through adduction reactions. Second, 1‐octene and n‐octane are separated with integrated distillation technology. The results indicated that this process can be utilized to recover 1‐octene from the C8 fraction of F–T synthetic products with a recovery yield of 93.6 % and an increase in purity from 65.6 to 98.7 wt%. As shown in Fig. 6(b), process modeling demonstrates that it trims energy consumption to 50.1% and total TAC to 55.1% in comparison with the traditional separation process. CONCLUSION: It was found that the integrated process can produce high‐purity 1‐octene, indicating that the integration process demonstrates clear potential advantages, such as reducing energy consumption and increasing economic value. This approach can be adopted on an industrial scale. © 2022 Society of Chemical Industry (SCI). [ABSTRACT FROM AUTHOR]
- Published
- 2022
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- View/download PDF
22. Xuebijing Injection Maintains GRP78 Expression to Prevent Candida albicans –Induced Epithelial Death in the Kidney.
- Author
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Shang, Ting, Yu, Qilin, Ren, Tongtong, Wang, Xin-Tong, Zhu, Hongyan, Gao, Jia-Ming, Pan, Guixiang, Gao, Xiumei, Zhu, Yan, Feng, Yuxin, and Li, Ming-Chun
- Subjects
CANDIDA albicans ,MYCOSES ,SEPTIC shock ,INTENSIVE care patients ,CANDIDEMIA ,COMMUNICABLE diseases ,SEPSIS - Abstract
Sepsis and septic shock threaten the survival of millions of patients in the intensive care unit. Secondary fungal infections significantly increased the risk of mortality in sepsis patients. Chinese medicine Xuebijing injection (XBJ) has been routinely used as an add-on treatment to sepsis and septic shock in China. Our network pharmacology analysis predicted that XBJ also influences fungal infection, consisting with results of pioneer clinical studies. We conducted in vivo and in vitro experiments to verify this prediction. To our surprise, XBJ rescued mice from lethal Candida sepsis in a disseminated Candida albicans infection model and abolished the colonization of C. albicans in kidneys. Although XBJ did not inhibit the growth and the virulence of C. albicans in vitro , it enhanced the viability of 293T cells upon C. albicans insults. Further RNA-seq analysis revealed that XBJ activated the endoplasmic reticulum (ER) stress pathway upon C. albicans infection. Western blot confirmed that XBJ maintained the expression of GRP78 in the presence of C. albicans. Interestingly, key active ingredients in XBJ (C0127) mirrored the effects of XBJ. C0127 not only rescued mice from lethal Candida sepsis and prevented the colonization of C. albicans in kidneys, but also sustained the survival of kidney epithelial cells partially by maintaining the expression of GRP78. These results suggested that XBJ may prevent fungal infection in sepsis patients. Pre-activation of ER stress pathway is a novel strategy to control C. albicans infection. Network pharmacology may accelerate drug development in the field of infectious diseases. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
- View/download PDF
23. A Review of the Developed New Model Biodiesels and Their Effects on Engine Combustion and Emissions.
- Author
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Liu, Shenghua, Chen, Wei, Zhu, Zengqiang, Jiang, Sa, Ren, Tongtong, and Guo, Hejun
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BIODIESEL fuels ,AUTOMOBILE engine combustion ,CARBON dioxide mitigation - Abstract
Biodiesel is regarded to be a renewable, CO
2 neutral and thus sustainable biological alternative diesel fuel. With attention to the reduction of petroleum import, PM 2.5 aerosol particles and the greenhouse effect gas CO2 , biodiesel has drawn great research interests and efforts in the past decade in China. Generally, biodiesel refers to fatty acid methyl ether (FAME) which has a proved effect in reducing diesel emission, particularly PM. However, FAME has a limited cetane number and oxygen content, to study the effects of elevated cetane number and oxygen content on fuel properties, engine combustion and emissions, ethylene glycol monomethyl ether is used to produce a series of new models of biodiesels by transesterification method. The feedstocks are rapeseed oil, soybean oil, peanut oil, palm oil and cottonseed oil. Ether group alcohols used in this study include ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, propylene glycol monomethyl ether, diethylene glycol monomethyl ether, triethylene glycol monomethyl ether. The molecular structure was proved by FT-IR and NMR analyses. Fuel properties were measured based on the corresponding standards. The developed new model biodiesels have cetane number (CN) over 70 and oxygen content over 17% by mass, which are higher than FAME (50 CN and 11% oxygen). They have the same level of lower heating value as FAME, but have a higher density, which helps to compensate the decrease of engine power. Meanwhile, the engine tests were carried out to investigate the effects of ether ester group on engine combustion and emissions. The test results show that FAME reduced smoke 30% to 50%, while the new model biodiesel fuels reduced engine smoke as high as 80% and have the potential to decrease engine HC, CO and NOx emissions 50% or more. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
24. Congee with soy-braised beef.
- Author
-
Knott, Bill
- Subjects
BEEF ,COOKING ,EGG yolk - Abstract
A recipe of "Congee with soy-braised beef and cured egg yolk" is presented.
- Published
- 2017
25. Digest.
- Subjects
RESTAURANTS ,MERGERS & acquisitions ,BUSINESS expansion - Abstract
The article presents news briefs related to restaurant industry. Brewery BrewDog has acquired the Draft House pub chain and will also open further bars. Lima restaurant in London, England, will stop its traditional a la karte menu and offer a more accessible and affordable selection of sharing dishes. Wasabi sushi and bento chain will be investing funds for expansion.
- Published
- 2018
26. Survival comparison between open and thoracoscopic esophagectomy for node-negative esophageal squamous cell cancer: an ambispective cohort study
- Author
-
Jia, Xiaocan, Ren, Tongtong, Chen, Peinan, Xin, Xin, Zhang, Yi, and Yang, Yongli
- Published
- 2024
- Full Text
- View/download PDF
27. New Waste Management Study Findings Recently Were Reported by Researchers at Nankai University (Transcription profiling-guided remodeling of sulfur metabolism in synthetic bacteria for efficiently capturing heavy metals)
- Subjects
Waste management ,Bacteria ,Physical fitness ,Heavy metals ,Sulfur ,Transcription (Genetics) ,Sulfur compounds ,Company business management ,Health ,Nankai University -- Management - Abstract
2020 SEP 5 (NewsRx) -- By a News Reporter-Staff News Editor at Obesity, Fitness & Wellness Week -- Current study results on Public Health - Waste Management have been published. [...]
- Published
- 2020
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