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17 results on '"Suga, Shinobu"'

8. Novel type aquaporin SIPs are mainly localized to the ER membrane and

Author

Ishikawa, Fumiyoshi, Suga, Shinobu, Uemura, Tomohiro, Sato, Masa H., and Maeshima, Masayoshi

Subjects
ER, Aquaporin, SIP, Arabidopsis, Water channel
Abstract

We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-β-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.

Published
2005

9. The Development Trend and the Land-Use Management in a Local Suburban Area - Based on an Analysis of Kiyotake-Town, JAPAN

Author

Yoshitake, Tetsunobu, Deguchi, Chikashi, and Suga, Shinobu

Subjects
Kiyotake, building permits, GIS, development trend, City Planning Law
Abstract

This paper aims to propose ideas to improve the land-use and management of Japanese suburban areas based on an investigation of development trends in Kiyotake town, a local town in the suburban area of a prefectural capital. Building permits during 1995 –1999 were analyzed by use of a geographic information system (GIS). The major results were as follows: 1) There were numerous undesirable developments in the urbanization control area and outside of the city planning area. 2) This was because of loose land-use management by the town authority, and loopholes in the conventional City Planning Law. 3) The City Planning Law 2000 has several features such as deregulation, decentralization and public involvement. However, the features have potentially deleterious effects. To achieve desirable land-use, stricter management should be adopted based on the strict stipulations and regulations of the Law. This management requires deliberate public involvement that attaches importance to the public interest., Proceedings of the Eastern Asia Society for Transportation Studies (EASTS '03), October 29-November 1, 2003, Fukuoka International Congress Center, Japan

Published
2003

16. Molecular Cloning, Water Channel Activity and Tissue Specific Expression of Two Isoforms of Radish Vacuolar Aquaporin1.

Author

Higuchi, Tatsuji, Suga, Shinobu, Tsuchiya, Tomohiro, Hisada, Hiromoto, Morishima, Shigeru, Okada, Yasunobu, and Maeshima, Masayoshi

Subjects
*RADISHES, *MOLECULAR cloning, *AQUAPORINS, *GENE expression in plants, *MEMBRANE proteins, *AMINO acid sequence, *XENOPUS, *COTYLEDONS
Abstract

A major membrane intrinsic protein (VM23) in vacuoles of radish (Raphanus) tap root was investigated. The cDNAs for two isoforms of VM23, γ- and δ-VM23, encode polypeptides of 253 and 248 amino acids, respectively, γ-and δ-VM23 correspond to the γ- and δ-TIP (tonoplast intrinsic protein) of Arabidopsis. The deduced amino acid sequences of the two VM23 isoforms were 60% identical. The ammo-terminal sequence of γ-VM23 showed agreement with the direct sequence of the purified VM23, suggesting that γ-VM23 is the most abundant molecule among the VM23 isoforms. When mRNAs of γ- and δ-VM23 were injected into Xenopus oocytes, the osmotic water permeability of oocytes increased 6-fold (60 to 200 μM s1) of the control oocytes. The transcripts of both isoforms were detected in a high level in growing hypocotyls and young leaves, but δ-VM23 was not detected in seedling roots. Light illumination enhanced the transcription of two genes of VM23 in cotyledons and roots but suppressed their expression in hypocotyls the growth of which was inhibited by light. These findings suggest that the expression of VM23 is tightly related to cell elongation [ABSTRACT FROM AUTHOR]

Published
1998
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17. The Development Trend and the Land-Use Management in a Local Suburban Area - Based on an Analysis of Kiyotake-Town, JAPAN

Abstract

type:Journal Article, This paper aims to propose ideas to improve the land-use and management of Japanese suburban areas based on an investigation of development trends in Kiyotake town, a local town in the suburban area of a prefectural capital. Building permits during 1995 –1999 were analyzed by use of a geographic information system (GIS). The major results were as follows: 1) There were numerous undesirable developments in the urbanization control area and outside of the city planning area. 2) This was because of loose land-use management by the town authority, and loopholes in the conventional City Planning Law. 3) The City Planning Law 2000 has several features such as deregulation, decentralization and public involvement. However, the features have potentially deleterious effects. To achieve desirable land-use, stricter management should be adopted based on the strict stipulations and regulations of the Law. This management requires deliberate public involvement that attaches importance to the public interest., Proceedings of the Eastern Asia Society for Transportation Studies (EASTS '03), October 29-November 1, 2003, Fukuoka International Congress Center, Japan

Published
2017

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