15 results on '"Soraya Djerbi"'
Search Results
2. Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses
- Author
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Ewa J. Mellerowicz, Nobuyuki Nishikubo, Pedro M. Coutinho, Matt Geisler, Stanislaw Karpinski, Junko Takahashi, Björn Sundberg, Jane Geisler-Lee, Soraya Djerbi, Henrik Aspeborg, Sara Andersson-Gunnerås, Leszek A. Kleczkowski, Bo Segerman, Emma R. Master, Tuula T. Teeri, and Bernard Henrissat
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Populus trichocarpa ,Expressed sequence tag ,biology ,Physiology ,fungi ,food and beverages ,Plant Science ,biology.organism_classification ,Biochemistry ,Arabidopsis ,Gene expression ,Glycosyltransferase ,Genetics ,biology.protein ,Sucrose synthase ,Glycoside hydrolase ,Gene - Abstract
Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and expansins. Poplar (Populus spp.) had approximately 1.6 times more CAZyme genes than Arabidopsis (Arabidopsis thaliana). Whereas most families were proportionally increased, xylan and pectin-related families were underrepresented and the GT1 family of secondary metabolite-glycosylating enzymes was overrepresented in poplar. CAZyme gene expression in poplar was analyzed using a collection of 100,000 expressed sequence tags from 17 different tissues and compared to microarray data for poplar and Arabidopsis. Expression of genes involved in pectin and hemicellulose metabolism was detected in all tissues, indicating a constant maintenance of transcripts encoding enzymes remodeling the cell wall matrix. The most abundant transcripts encoded sucrose synthases that were specifically expressed in wood-forming tissues along with cellulose synthase and homologs of KORRIGAN and ELP1. Woody tissues were the richest source of various other CAZyme transcripts, demonstrating the importance of this group of enzymes for xylogenesis. In contrast, there was little expression of genes related to starch metabolism during wood formation, consistent with the preferential flux of carbon to cell wall biosynthesis. Seasonally dormant meristems of poplar showed a high prevalence of transcripts related to starch metabolism and surprisingly retained transcripts of some cell wall synthesis enzymes. The data showed profound changes in CAZyme transcriptomes in different poplar tissues and pointed to some key differences in CAZyme genes and their regulation between herbaceous and woody plants.
- Published
- 2006
3. The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes
- Author
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Mats Lindskog, Tuula T. Teeri, Lars Arvestad, Fredrik Sterky, and Soraya Djerbi
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Expressed Sequence Tags ,Whole genome sequencing ,Genetics ,Populus trichocarpa ,DNA, Complementary ,Phylogenetic tree ,cDNA library ,Chromosome Mapping ,Plant Science ,Biology ,biology.organism_classification ,Genome ,Chromosomes, Plant ,Populus ,Glucosyltransferases ,Complementary DNA ,Gene expression ,Gene ,Conserved Sequence ,Genome, Plant ,Phylogeny - Abstract
The genome sequence of Populus trichocarpa was screened for genes encoding cellulose synthases by using full-length cDNA sequences and ESTs previously identified in the tissue specific cDNA libraries of other poplars. The data obtained revealed 18 distinct CesA gene sequences in P. trichocarpa. The identified genes were grouped in seven gene pairs, one group of three sequences and one single gene. Evidence from gene expression studies of hybrid aspen suggests that both copies of at least one pair, CesA3-1 and CesA3-2, are actively transcribed. No sequences corresponding to the gene pair, CesA6-1 and CesA6-2, were found in Arabidopsis or hybrid aspen, while one homologous gene has been identified in the rice genome and an active transcript in Populus tremuloides. A phylogenetic analysis suggests that the CesA genes previously associated with secondary cell wall synthesis originate from a single ancestor gene and group in three distinct subgroups. The newly identified copies of CesA genes in P. trichocarpa give rise to a number of new questions concerning the mechanism of cellulose synthesis in trees.
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- 2005
4. Carbohydrate-Active Enzymes Involved in the Secondary Cell Wall Biogenesis in Hybrid Aspen
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Pedro M. Coutinho, Ewa J. Mellerowicz, Peter Nilsson, Mark Stam, Henrik Aspeborg, Bernard Henrissat, Björn Sundberg, Jarmo Schrader, Bahram Amini, Emma R. Master, Åsa M. Kallas, Soraya Djerbi, Fredrik Sterky, Göran Sandberg, Stuart E. Denman, and Tuula T. Teeri
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chemistry.chemical_classification ,Expressed sequence tag ,Physiology ,Plant Science ,Biology ,Xylan acetylation ,Enzyme ,chemistry ,Biochemistry ,Complementary DNA ,Glycosyltransferase ,Genetics ,biology.protein ,Glycoside hydrolase ,DNA microarray ,Functional genomics - Abstract
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the key structural carbohydrates in wood fibers remain obscure. We have used a functional genomics approach to identify enzymes involved in carbohydrate biosynthesis and remodeling during xylem development in the hybrid aspen Populus tremula × tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were hybridized with probes obtained from narrow tissue sections prepared by cryosectioning of the developing xylem. Bioinformatic analyses using the sensitive tools developed for carbohydrate-active enzymes allowed the identification of 25 xylem-specific glycosyltransferases belonging to the Carbohydrate-Active EnZYme families GT2, GT8, GT14, GT31, GT43, GT47, and GT61 and nine glycosidases (or transglycosidases) belonging to the Carbohydrate-Active EnZYme families GH9, GH10, GH16, GH17, GH19, GH28, GH35, and GH51. While no genes encoding either polysaccharide lyases or carbohydrate esterases were found among the secondary wall-specific genes, one putative O-acetyltransferase was identified. These wood-specific enzyme genes constitute a valuable resource for future development of engineered fibers with improved performance in different applications.
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- 2005
5. In vitro synthesis of (13)---glucan (callose) and cellulose by detergent extracts of membranes from cell suspension cultures of hybrid aspen
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Audrey Colombani, Anna B. Ohlsson, Kristina Blomqvist, Torkel Berglund, Soraya Djerbi, Laurence Bessueille, Tuula T. Teeri, and Vincent Bulone
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Materials science ,Chromatography ,Polymers and Plastics ,Callose ,Cellobiose ,MOPS ,Cell wall ,chemistry.chemical_compound ,Hydrolysis ,Digitonin ,Membrane ,chemistry ,Biochemistry ,Cellulose - Abstract
The aim of this work was to optimize the conditions for in vitro synthesis of (1→3)-β-D-glucan (callose) and cellulose, using detergent extracts of membranes from hybrid aspen (Populus tremula × tremuloides) cells grown as suspension cultures. Callose was the only product synthesized when CHAPS extracts were used as a source of enzyme. The optimal reaction mixture for callose synthesis contained 100 mM Mops buffer pH 7.0, 1 mM UDP-glucose, 8 mM Ca2+, and 20 mM cellobiose. The use of digitonin to extract the membrane-bound proteins was required for cellulose synthesis. Yields as high as 50% of the total in vitro products were obtained when cells were harvested in the stationary phase of the growth curve, callose being the other product. The optimal mixture for cellulose synthesis consisted of 100 mM Mops buffer pH 7.0, 1 mM UDP-glucose, 1 mM Ca2+, 8 mM Mg2+, and 20 mM cellobiose. The in vitroβ-glucans were identified by hydrolysis of radioactive products, using specific enzymes. 13C-Nuclear magnetic resonance spectroscopy and transmission electron microscopy were also used for callose characterization. The (1→3)-β-D-glucan systematically had a microfibrillar morphology, but the size and organization of the microfibrils were affected by the nature of the detergent used for enzyme extraction. The discussion of the results is included in a short review of the field that also compares the data obtained with those available in the literature. The results presented show that the hybrid aspen is a promising model for in vitro studies on callose and cellulose synthesis.
- Published
- 2004
6. Identification and expression analysis of genes encoding putative cellulose synthases (CesA) in the hybrid aspen, Populus tremula (L.) P. tremuloides (Michx.)
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Soraya Djerbi, Peter Nilsson, Ewa J. Mellerowicz, Tuula T. Teeri, Henrik Aspeborg, Kristina Blomqvist, and Björn Sundberg
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Polymers and Plastics ,ATP synthase ,biology ,Protein subunit ,Xylem ,Isozyme ,Cell wall ,Gene expression profiling ,chemistry.chemical_compound ,chemistry ,Biochemistry ,biology.protein ,Cellulose ,Gene - Abstract
Cellulose is synthesized in plant cell walls by large membrane-bound protein complexes proposed to contain several copies of the catalytic subunit of the cellulose synthase, CesA. Here we report identification of 10 distinct CesA genes within a database of 100,000 ESTs of the hybrid aspen, Populus tremula (L.) × P. tremuloides (Michx.). Expression analyses in normal wood undergoing xylogenesis and in tension wood indicate xylem specific expression of four putative CesA isoenzymes, PttCesA1, PttCesA3-1, PttCesA3-2 and PttCesA9. Both the protein sequences and the expression profiles of PttCesA3-1 and PttCesA3-2 are very similar, and they may thus represent redundant copies of an enzyme with essentially the same function. Further, one of the generally more constitutively expressed CesA genes, PttCesA2, seems to be activated on the opposite side of a tension wood induced stem, while PttCesA6 appears to be more specific for leaf tissues. The rest of the hybrid aspen CesA genes were found to be relatively evenly expressed over the poplar tissues hereby studied.
- Published
- 2004
7. Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis
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Vincent Bulone, Soraya Djerbi, Nicolas Sindt, Michel Guichardant, Laurence Bessueille, Tuula T. Teeri, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Régulations métaboliques, nutrition et diabètes - UM55 (RMND UM55), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), School of Biotechnology, Royal Institute of Technology [Stockholm] (KTH ), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Octoxynol ,MESH: Membrane Microdomains ,Biology ,Hybrid Cells ,Polysaccharide ,Endocytosis ,01 natural sciences ,Biochemistry ,Trees ,Cell wall ,03 medical and health sciences ,chemistry.chemical_compound ,Membrane Microdomains ,MESH: Cell Wall ,Biosynthesis ,Cell Wall ,Polysaccharides ,MESH: Octoxynol ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Molecular Biology ,Lipid raft ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,MESH: Glucosyltransferases ,Callose ,Cell Biology ,Sphingolipid ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,MESH: Trees ,Membrane ,MESH: Polysaccharides ,chemistry ,Glucosyltransferases ,MESH: Hybrid Cells ,010606 plant biology & botany - Abstract
International audience; Detergent-resistant plasma membrane microdomains [DRMs (detergent-resistant membranes)] were isolated recently from several plant species. As for animal cells, a large range of cellular functions, such as signal transduction, endocytosis and protein trafficking, have been attributed to plant lipid rafts and DRMs. The data available are essentially based on proteomics and more approaches need to be undertaken to elucidate the precise function of individual populations of DRMs in plants. We report here the first isolation of DRMs from purified plasma membranes of a tree species, the hybrid aspen Populus tremula x tremuloides, and their biochemical characterization. Plasma membranes were solubilized with Triton X-100 and the resulting DRMs were isolated by flotation in sucrose density gradients. The DRMs were enriched in sterols, sphingolipids and glycosylphosphatidylinositol-anchored proteins and thus exhibited similar properties to DRMs from other species. However, they contained key carbohydrate synthases involved in cell wall polysaccharide biosynthesis, namely callose [(1-->3)-beta-D-glucan] and cellulose synthases. The association of these enzymes with DRMs was demonstrated using specific glucan synthase assays and antibodies, as well as biochemical and chemical approaches for the characterization of the polysaccharides synthesized in vitro by the isolated DRMs. More than 70% of the total glucan synthase activities present in the original plasma membranes was associated with the DRM fraction. In addition to shedding light on the lipid environment of callose and cellulose synthases, our results demonstrate the involvement of DRMs in the biosynthesis of important cell wall polysaccharides. This novel concept suggests a function of plant membrane microdomains in cell growth and morphogenesis.
- Published
- 2009
8. Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity
- Author
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Jamel Bouzenzana, Johanna Fugelstad, Vincent Bulone, Tuula T. Teeri, Gea Guerriero, Lars Arvestad, Ines Ezcurra, and Soraya Djerbi
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Biology ,Saprolegnia ,Microbiology ,Cell wall ,chemistry.chemical_compound ,DNA, Algal ,Chitin ,Stress, Physiological ,Gene expression ,Nitriles ,Genetics ,Cellulose ,Enzyme Inhibitors ,Gene ,Oomycete ,ATP synthase ,Algal Proteins ,Congo Red ,Chitin synthase ,biology.organism_classification ,Blotting, Southern ,chemistry ,Biochemistry ,Gene Expression Regulation ,Glucosyltransferases ,biology.protein - Abstract
Cellulose biosynthesis is a vital but yet poorly understood biochemical process in Oomycetes. Here, we report the identification and characterization of the cellulose synthase genes (CesA) from Saprolegnia monoica. Southern blot experiments revealed the occurrence of three CesA homologues in this species and phylogenetic analyses confirmed that Oomycete CesAs form a clade of their own. All gene products contained the D,D,D,QXXRW signature of most processive glycosyltransferases, including cellulose synthases. However, their N-terminal ends exhibited Oomycete-specific domains, i.e. Pleckstrin Homology domains, or conserved domains of an unknown function together with additional putative transmembrane domains. Mycelial growth was inhibited in the presence of the cellulose biosynthesis inhibitors 2,6-dichlorobenzonitrile or Congo Red. This inhibition was accompanied by a higher expression of all CesA genes in the mycelium and increased in vitro glucan synthase activities. Altogether, our data strongly suggest a direct involvement of the identified CesA genes in cellulose biosynthesis.
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- 2009
9. HMG-CoA reductase expression in breast cancer is associated with a less aggressive phenotype and influenced by anthropometric factors
- Author
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Alexander Gaber, Göran Landberg, Lola Anagnostaki, Jonas Manjer, Signe Borgquist, Malin Goldman, Karin Jirström, Soraya Djerbi, and Fredrik Pontén
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Cancer Research ,medicine.medical_specialty ,medicine.drug_class ,medicine.medical_treatment ,Abdominal Fat ,Breast Neoplasms ,Gene Expression Regulation, Enzymologic ,Diet and cancer ,Breast cancer ,Risk Factors ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Proportional Hazards Models ,biology ,business.industry ,Waist-Hip Ratio ,Incidence ,Estrogen Replacement Therapy ,Cancer ,Hormone replacement therapy (menopause) ,Middle Aged ,medicine.disease ,Hydroxymethylglutaryl-CoA reductase ,Immunohistochemistry ,Gene Expression Regulation, Neoplastic ,Endocrinology ,Phenotype ,Oncology ,Estrogen ,HMG-CoA reductase ,biology.protein ,Female ,Hydroxymethylglutaryl CoA Reductases ,Breast disease ,business - Abstract
Although several studies have reported on the anti-tumoural properties exerted by 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoAR) inhibitors (statins), the in vivo expression of HMG-CoAR in human cancer has been considerably less investigated. In our study, we examined the immunohistochemical expression of HMG-CoAR in 511 incident breast cancers within the Malmö Diet and Cancer Study in order to explore its relationship to established clinicopathological and tumour biological parameters. Furthermore, the potential influence of estrogen exposure on HMG-CoAR expression was assessed by performing Cox's proportional hazards analyses of the relationship between the use of hormone replacement therapy (HRT), obesity (waist circumference) and tumour-cell specific HMG-CoAR expression. We found that HMG-CoAR was present in various fractions and intensities in the cytoplasm, sometimes with a membranous pattern, but not in the tumour cell nuclei. The expression of HMG-CoAR was associated with a smaller tumour size (p = 0.02), low histological grade (p = 0.001), low Ki67 index (p = 0.004), ERalpha+ (p = 0.02), ERbeta+ (p = 0.005), and high p27 expression (p =0.001). The incidence of tumours with a high HMG-CoAR-expression was increased among HRT-users, although this was not statistically significant in a heterogeneity analysis. Obesity was significantly associated with a high HMG-CoAR expression assessed both as a high (50%) fraction of positive cells (relative risk: 2.06; 95% confidence interval: 1.20-3.51), and a strong staining intensity (2.33: 1.08-5.02). In summary, we demonstrate that HMG-CoAR is differentially expressed in breast cancer and that a high expression is associated with prognostically favourable tumour parameters. Moreover, estrogen related life-style and anthropometric factors might indeed regulate HMG-CoAR expression.
- Published
- 2008
10. Cellulose Biosynthesis in Forest Trees
- Author
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Tuula T. Teeri, Henrik Aspeborg, Kristina Blomqvist, and Soraya Djerbi
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technology, industry, and agriculture ,Biology ,complex mixtures ,Cellulose microfibril ,Cell wall ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Botany ,Lignin ,Hemicellulose ,Cellulose ,Secondary cell wall ,Functional genomics ,Gene - Abstract
Wood formation is a fundamental biological process of significant economic andcommercial interest. During wood formation, most glucose from the carbohydratemetabolism is channeled to cellulose in the secondary cell walls. The cellulose microfibrils associate with hemicellulose, proteins, and lignin to form the strong and flexiblebiocomposite known as wood. As the main wood component, cellulose is essential forthe survival of trees and for their exploitation by man.In spite of this, the molecular details of cellulose biosynthesis have remained obscure in all plants. In particular, the toughness of wood cells makes it hard to isolateactive enzymes and study cellulose synthesis in trees. Functional genomics providespowerful new tools to study complex metabolic processes. In this way, 18 CesA geneshave been recently identified in the genome sequence of Populus trichocarpa.Expression profiling during wood formation has shown that four of these genesare specifically upregulated during xylogenesis and/or tension wood formation. Othergenes that follow the same expression pattern as the wood-related CesA genes encodethe putative Korrigan ortholog PttCel9A and a novel microtubule associated proteinPttMAP20. Cell suspension cultures of hybrid aspen with elevated expression of thesecondary cell wall specific PttCesA genes have been used for efficient in vitro synthesisof cellulose, which will facilitate future studies of this challenging process in trees.
- Published
- 2007
11. Cell suspension cultures of Populus tremula x tremuloides exhibit a high level of cellulose synthase gene expression that coincides with increased in vitro cellulose synthase activity
- Author
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Kristina Blomqvist, Laurence Bessueille, Tuula T. Teeri, Veronika Ståldal, Anders Winzell, Soraya Djerbi, Vincent Bulone, Xinguo Li, Anna B. Ohlsson, Torkel Berglund, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Centre National de la Recherche Scientifique (CNRS)-École Supérieure Chimie Physique Électronique de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon
- Subjects
0106 biological sciences ,beta-Glucans ,Cell ,Plant Science ,Biology ,Calcofluor-white ,Cellulose synthase activity ,01 natural sciences ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,chemistry.chemical_compound ,Cellulase ,Gene Expression Regulation, Plant ,Gene expression ,medicine ,RNA, Messenger ,Cellulose ,Cells, Cultured ,Plant Proteins ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Reverse Transcriptase Polymerase Chain Reaction ,Benzenesulfonates ,Callose ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,General Medicine ,Populus ,medicine.anatomical_structure ,Enzyme ,chemistry ,Biochemistry ,Glucosyltransferases ,Hybridization, Genetic ,Cell aging ,010606 plant biology & botany - Abstract
Compared to wood, cell suspension cultures provide convenient model systems to study many different cellular processes in plants. Here we have established cell suspension cultures of Populus tremula L. x P. tremuloides Michx. and characterized them by determining the enzymatic activities and/or mRNA expression levels of selected cell wall-specific proteins at the different stages of growth. While enzymes and proteins typically associated with primary cell wall synthesis and expansion were detected in the exponential growth phase of the cultures, the late stationary phase showed high expression of the secondary-cell-wall-associated cellulose synthase genes. Interestingly, detergent extracts of membranes from aging cell suspension cultures exhibited high levels of in vitro cellulose synthesis. The estimated ratio of cellulose to callose was as high as 50 : 50, as opposed to the ratio of 30 : 70 so far achieved with membrane preparations extracted from other systems. The increased cellulose synthase activity was also evidenced by higher levels of Calcofluor white binding in the cell material from the stationary-phase cultures. The ease of handling cell suspension cultures and the improved capacity for in vitro cellulose synthesis suggest that these cultures offer a new basis for studying the mechanism of cellulose biosynthesis.
- Published
- 2006
12. Plasma membrane microdomains from hybrid aspen cells are involved in cell wall polysaccharide biosynthesis.
- Author
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Laurence Bessueille, Nicolas Sindt, Soraya Djerbi, Tuula T. Teeri, and Vincent Bulone
- Subjects
PLANT plasma membranes ,POLYSACCHARIDE synthesis ,PLANT cellular signal transduction ,PROTEOMICS ,ENDOCYTOSIS ,EUROPEAN aspen - Abstract
Detergent-resistant plasma membrane microdomains [DRMs (detergent-resistant membranes)] were isolated recently from several plant species. As for animal cells, a large range of cellular functions, such as signal transduction, endocytosis and protein trafficking, have been attributed to plant lipid rafts and DRMs. The data available are essentially based on proteomics and more approaches need to be undertaken to elucidate the precise function of individual populations of DRMs in plants. We report here the first isolation of DRMs from purified plasma membranes of a tree species, the hybrid aspen Populus tremula × tremuloides, and their biochemical characterization. Plasma membranes were solubilized with Triton X-100 and the resulting DRMs were isolated by flotation in sucrose density gradients. The DRMs were enriched in sterols, sphingolipids and glycosylphosphatidylinositol-anchored proteins and thus exhibited similar properties to DRMs from other species. However, they contained key carbohydrate synthases involved in cell wall polysaccharide biosynthesis, namely callose [(1→3)-β-D-glucan] and cellulose synthases. The association of these enzymes with DRMs was demonstrated using specific glucan synthase assays and antibodies, as well as biochemical and chemical approaches for the characterization of the polysaccharides synthesized in vitro by the isolated DRMs. More than 70% of the total glucan synthase activities present in the original plasma membranes was associated with the DRM fraction. In addition to shedding light on the lipid environment of callose and cellulose synthases, our results demonstrate the involvement of DRMs in the biosynthesis of important cell wall polysaccharides. This novel concept suggests a function of plant membrane microdomains in cell growth and morphogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
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13. Cell suspension cultures of Populus tremula × P. tremuloides exhibit a high level of cellulose synthase gene expression that coincides with increased in vitro cellulose synthase activity.
- Author
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Anna B. Ohlsson, Soraya Djerbi, Anders Winzell, Laurence Bessueille, Veronika Ståldal, Xinguo Li, Kristina Blomqvist, Vincent Bulone, Tuula T. Teeri, and Torkel Berglund
- Subjects
PLANT cell culture ,EUROPEAN aspen ,MESSENGER RNA ,PLANT cell walls ,CELLULOSE - Abstract
Summary. Compared to wood, cell suspension cultures provide convenient model systems to study many different cellular processes in plants. Here we have established cell suspension cultures of Populus tremula L. × P. tremuloides Michx. and characterized them by determining the enzymatic activities and/or mRNA expression levels of selected cell wall-specific proteins at the different stages of growth. While enzymes and proteins typically associated with primary cell wall synthesis and expansion were detected in the exponential growth phase of the cultures, the late stationary phase showed high expression of the secondary-cell-wall-associated cellulose synthase genes. Interestingly, detergent extracts of membranes from aging cell suspension cultures exhibited high levels of in vitro cellulose synthesis. The estimated ratio of cellulose to callose was as high as 50 : 50, as opposed to the ratio of 30 : 70 so far achieved with membrane preparations extracted from other systems. The increased cellulose synthase activity was also evidenced by higher levels of Calcofluor white binding in the cell material from the stationary-phase cultures. The ease of handling cell suspension cultures and the improved capacity for in vitro cellulose synthesis suggest that these cultures offer a new basis for studying the mechanism of cellulose biosynthesis. [ABSTRACT FROM AUTHOR]
- Published
- 2006
14. Contents to Volume 11 (2004).
- Subjects
CELLULOSE - Abstract
The tables of contents for various numbers of Volume 11, 2004 issue of the periodical "Cellulose" are presented.
- Published
- 2004
15. Cellulose: Molecular and Structural Biology : Selected Articles on the Synthesis, Structure, and Applications of Cellulose
- Author
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R. Malcolm Jr. Brown, Inder M. Saxena, R. Malcolm Jr. Brown, and Inder M. Saxena
- Subjects
- Polymers, Biochemistry, Cellulose--Chemistry, Cellulose--Synthesis
- Abstract
Cellulose: Molecular and Structural Biology is an up-to-date treatise on the most advanced and provocative research into the biosynthesis, structure, and applications of Nature's most abundant macromolecule and renewable resource, cellulose. Molecular, biochemical, and evolutionary aspects of cellulose biosynthesis are reviewed in a variety of living organisms, including cyanobacteria, eubacteria, (Acetobacter, Salmonella, and E. coli), vascular plants (including Arabidopsis, forest trees, and maize), and tunicates. Phylogenetic analysis, molecular genetics, and the potential for metabolic engineering are also presented. Novel structural approaches include the macromolecular structure of the synthesizing units, the terminal complexes as well as the cellulose product in its many forms are also included. Novel applications using cellulose include smart materials, carbonised cellulose, and biomedical applications. First hand information from theleading researchers distinguishes this work from other books on cellulose.
- Published
- 2007
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