186 results on '"Shevchenko LS"'
Search Results
2. Synthesis and Evaluation of Antimicrobial and Cytotoxic Activity of Oxathiine-Fused Quinone-Thioglucoside Conjugates of Substituted 1,4-Naphthoquinones.
- Author
-
Sabutski YE, Menchinskaya ES, Shevchenko LS, Chingizova EA, Chingizov AR, Popov RS, Denisenko VA, Mikhailov VV, Aminin DL, and Polonik SG
- Subjects
- Anti-Infective Agents chemical synthesis, Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Chemistry Techniques, Synthetic, HeLa Cells, Humans, Thioglucosides chemistry, Naphthoquinones chemistry, Oxathiins chemistry, Quinones chemistry, Thioglucosides chemical synthesis, Thioglucosides pharmacology
- Abstract
A series of new tetracyclic oxathiine-fused quinone-thioglycoside conjugates based on biologically active 1,4-naphthoquinones and 1-mercapto derivatives of per- O -acetyl d-glucose, d-galactose, d-xylose, and l-arabinose have been synthesized, characterized, and evaluated for their cytotoxic and antimicrobial activities. Six tetracyclic conjugates bearing a hydroxyl group in naphthoquinone core showed high cytotoxic activity with EC
50 values in the range of 0.3 to 0.9 μM for various types of cancer and normal cells and no hemolytic activity up to 25 μM. The antimicrobial activity of conjugates was screened against Gram-positive bacteria ( Staphylococcus aureus , Bacillus cereus ), Gram-negative bacteria ( Pseudomonas aeruginosa and Escherichia coli ), and fungus Candida albicans by the agar diffusion method. The most effective juglone conjugates with d-xylose or l-arabinose moiety and hydroxyl group at C-7 position of naphthoquinone core at concentration 10 µg/well showed antimicrobial activity comparable with antibiotics vancomicin and gentamicin against Gram-positive bacteria strains. In liquid media, juglone-arabinosidic tetracycles showed highest activity with MIC 6.25 µM. Thus, a positive effect of heterocyclization with mercaptosugars on cytotoxic and antimicrobial activity for group of 1,4-naphthoquinones was shown.- Published
- 2020
- Full Text
- View/download PDF
3. Non-Specific Porins of Yersinia pseudotuberculosis as Inductors of Experimental Hyperthyroidism in Mice.
- Author
-
Portnyagina OY, Golotin VA, Zelepuga EA, Khomenko VA, Shevchenko LS, and Novikova OD
- Subjects
- Animals, Antibodies, Bacterial administration & dosage, Antigens, Bacterial administration & dosage, Antigens, Bacterial chemistry, Female, Hyperthyroidism immunology, Hyperthyroidism metabolism, Immunization, Mice, Mice, Inbred BALB C, Porins administration & dosage, Porins chemistry, Protein Multimerization, Receptors, Thyrotropin immunology, Receptors, Thyrotropin metabolism, Recombinant Proteins administration & dosage, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Thyroxine biosynthesis, Yersinia pseudotuberculosis immunology, Antigens, Bacterial immunology, Hyperthyroidism chemically induced, Porins immunology, Yersinia pseudotuberculosis chemistry
- Abstract
In vivo experiments showed that antibodies to OmpC and OmpF porins of Yersinia pseudotuberculosis increased thyroxine (T4) level in the blood of experimental animals. The mice were immunized with different antigens: recombinant OmpF porin in a soluble monomeric form, trimers of OmpC and OmpF porins isolated from the outer membrane, or antibodies to them. The level of thyroxine in the blood of mice immunized with OmpF and OmpC porins increased by 5.47 and 22.3 times, respectively; after immunization with antibodies to these proteins, blood thyroxine increased by 9.28 and 14.29 times. Immunization with recombinant OmpF porin induced no reliable increase in thyroxine level. Hence, the serum to recombinant OmpF porin contains no antibodies specific to conformational antigenic determinants that are present in the protein trimer and, according to our previous findings from molecular docking studies, determine cross-reactions between OmpF porin of Y. pseudotuberculosis and thyroidstimulating hormone receptor.
- Published
- 2019
- Full Text
- View/download PDF
4. A view of Russian specialists on the quality control system of psychiatric care
- Author
-
Shevchenko Ls and Solokhina Ta
- Subjects
Psychiatry ,Quality Control ,medicine.medical_specialty ,business.industry ,Russia ,Psychiatry and Mental health ,Quality control system ,Physicians ,Environmental health ,Family medicine ,Humans ,Medicine ,Neurology (clinical) ,business ,Specialization - Published
- 2015
- Full Text
- View/download PDF
5. [A view of Russian specialists on the quality control system of psychiatric care].
- Author
-
Solokhina TA and Shevchenko LS
- Subjects
- Humans, Russia, Physicians, Psychiatry standards, Quality Control, Specialization
- Published
- 2015
- Full Text
- View/download PDF
6. Comprehensive evaluation of the remediation efficacy of composted and uncomposted mushroom substrate on lead-contaminated soil.
- Author
-
Yuan, Yaru, Zhu, Lin, Jin, Yu, Xu, Xiuhong, Liu, Xuesheng, and Qu, Juanjuan
- Abstract
Spent mushroom substrate (SMS) is a kind of agricultural waste currently used as amendment, however, its remediation efficacy on heavy metal-contaminated soil has not been comprehensively evaluated. In this study, the effects of SMS and its compost (CSMS) on soil environment (including lead availability, physicochemical properties, nutrient content, enzyme activity, bacterial community) and plant growth (including germination, photosynthesis, and biomass) in lead-contaminated soil were investigated. Independent germination test and pot experiments of water spinach were carried out under 600 mg/kg lead contamination and 5% amendment dosage. The results showed that SMS increased the germination rate by 45.1% and CSMS decreased it by 60.9%. Both amendments enhanced the photosynthesis and increased the fresh weight of water spinach by more than 12.6% in polluted soil, but SMS had a negative effect on photosynthesis and decreased the fresh weight in unpolluted soil. The SMS and CSMS reduced soil lead availability and thus decreased the lead content of water spinach by more than 6.1%. SMS increased soil pH, while CSMS decreased it. CSMS increased soil conductivity, organic carbon, and available N more than SMS. Both amendments consistently and positively impacted on soil enzymes and bacteria. In conclusion, composting is beneficial to increase the available N of SMS, which prevents SMS from competing with plants for N during mineralization. However, CSMS should not be directly used in the sowing period, because its alkalinity and high salt content may inhibit seed germination. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. [Psychiatric hospital: past, present and prospects].
- Author
-
Iastrebov VS, Limankin OV, Solokhina TA, Shevchenko LS, Mitikhin VG, and Mitikhina IA
- Subjects
- History, 19th Century, History, 20th Century, History, 21st Century, Humans, Hospitals, Psychiatric history, Hospitals, Psychiatric organization & administration, Hospitals, Psychiatric trends
- Abstract
Academic, clinical, social, economic, organizational and other aspects of psychiatric hospitals are discussed in the main sections of the article: development and current state of medical care, prospect of inpatient care. Main measures for the improvement of the activity of psychiatric hospital, their interaction with outpatient organizations are presented. The necessity of improving the image of a psychiatric hospital is emphasized.
- Published
- 2014
8. [Inpatient psychiatric care in the Russian Federation: organizational and economic aspects].
- Author
-
Shevchenko LS, Iastrebov VS, Solokhina TA, Mitikhin VG, and Tvorogova NA
- Subjects
- Healthcare Financing, Hospitals, Psychiatric organization & administration, Hospitals, Psychiatric statistics & numerical data, Humans, Russia, Hospitals, Psychiatric economics, Inpatients statistics & numerical data
- Published
- 2013
9. [Actual problems of inpatient psychiatric care in Russia].
- Author
-
Iastrebov VS, Mitikhin VG, Solokhina TA, Shevchenko LS, and Tvorogova NA
- Subjects
- Humans, Russia, Hospitals, Psychiatric, Inpatients, Mental Disorders therapy, Mental Health Services organization & administration, Psychiatry organization & administration
- Abstract
A comparative evaluation of inpatient psychiatric care in Russia and some other countries is presented. A systematic analysis of the performance of psychiatric hospitals is conducted. The process of the deinstitutionalization in Russian psychiatry is highlighted. A range of problems hindering a reform of inpatient psychiatric service of the country is singled out.
- Published
- 2013
10. Structure of the O-specific polysaccharide of the marine bacterium Arenibacter palladensis KMM 3961(T) containing 2-acetamido-2-deoxy-l-galacturonic acid.
- Author
-
Tomshich SV, Isakov VV, Komandrova NA, and Shevchenko LS
- Subjects
- Carbohydrate Sequence, Lipopolysaccharides metabolism, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Flavobacteriaceae metabolism, Hexuronic Acids chemistry, O Antigens chemistry
- Abstract
The O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Arenibacter palladensis type strain KMM 3961(T) and studied by chemical methods and (1)H and (13)C NMR spectroscopy including 2D COSY, TOCSY, (1)H,(13)C HSQC, and HMBC experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units containing two mannose residues (Man), one 2-acetamido-2-deoxy-D-galactose residue (D-GalNAc), and one 2-acetamido-2-deoxy-L-galacturonic acid residue (L-GalNAcA) and having the following structure: →2)-α-D-Manp-(1→6)-α-D-Manp-(1→4)-α-L-GalpNAcA-(1→3)-β-D-GalpNAc-(1→.
- Published
- 2012
- Full Text
- View/download PDF
11. [Mental health of world's population: social and economic aspects (a literature review 2000-2010)].
- Author
-
Iastrebov VS, Mitikhina IA, Mitikhin VG, Shevchenko LS, and Solokhina TA
- Subjects
- Africa epidemiology, Australia epidemiology, Europe epidemiology, Humans, North America epidemiology, Socioeconomic Factors, Global Health, Mental Disorders epidemiology, Mental Health, Population
- Abstract
The authors had analyzed current approaches and results of the evaluation of the economic burden of mental health in the world derived from foreign research in 2000-2010. Sources of information were publications of the WHO database and the WHO regional offices, the database of the National Library of Medicine U.S. "MedLine", archives of publications by leading foreign medical journals in the field of mental health. The burden of mental illnesses, especially depression and anxiety disorders, has been increasing in all regions of the world. It has been shown that obtaining reliable estimates of mental health in modern conditions is only possible on the basis of reliable epidemiological, clinical and social/economic data which requires the development and improvement of mental health services as well as relevant social, public and private entities.
- Published
- 2012
12. Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid.
- Author
-
Nazarenko EL, Perepelov AV, Shevchenko LS, Daeva ED, Ivanova EP, Shashkov AS, and Widmalm G
- Subjects
- Carbohydrate Sequence, Molecular Sequence Data, Nuclear Magnetic Resonance, Biomolecular, Shewanella immunology, Sugar Acids analysis, O Antigens chemistry, Shewanella chemistry
- Abstract
Structure of the O-specific polysaccharide chain of the lipopolysaccharide (LPS) of Shewanella japonica KMM 3601 was elucidated. The initial and O-deacylated LPS as well as a trisaccharide representing the O-deacetylated repeating unit of the O-specific polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The polysaccharide was found to contain a rare higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid (a derivative of 4-epilegionaminic acid, 4eLeg). The following structure of the trisaccharide repeating unit was established: →4)-α-4eLegp5Ac7Ac-(2→4)-β-D-GlcpA3Ac-(1→3)-β-D-GalpNAc-(1→.
- Published
- 2011
- Full Text
- View/download PDF
13. Salinimicrobium marinum sp. nov., a halophilic bacterium of the family Flavobacteriaceae, and emended descriptions of the genus Salinimicrobium and Salinimicrobium catena.
- Author
-
Nedashkovskaya OI, Vancanneyt M, Kim SB, Han J, Zhukova NV, and Shevchenko LS
- Subjects
- Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Flavobacteriaceae genetics, Flavobacteriaceae physiology, Locomotion, Molecular Sequence Data, Phylogeny, Pigments, Biological biosynthesis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sodium Chloride metabolism, Temperature, Flavobacteriaceae classification, Flavobacteriaceae isolation & purification, Seawater microbiology
- Abstract
Two novel heterotrophic, facultatively anaerobic, gliding and yellow-pigmented bacteria, designated strains KMM 6270(T) and KMM 6320, were isolated from different marine environments and studied using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis placed the strains within the family Flavobacteriaceae. Strains KMM 6270(T) and KMM 6320 were most closely related to the type strains of recognized species of the genus Salinimicrobium (95.0-96.6 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40-41 mol%. The strains grew with 0.5-15 % (w/v) NaCl (optimum 4 % NaCl) and at 4-41 °C (optimum 28-32 °C). Aesculin and gelatin were hydrolysed, but agar, casein, DNA and chitin were not. The phylogenetic data taken together with the results of the genotypic and phenotypic studies permit the classification of strains KMM 6270(T) and KMM 6320 as members of a novel species of the genus Salinimicrobium, for which the name Salinimicrobium marinum sp. nov. is proposed. The type strain is KMM 6270(T) (=KCTC 12719(T)=LMG 25395(T)).
- Published
- 2010
- Full Text
- View/download PDF
14. Pseudozobellia thermophila gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae, isolated from the green alga Ulva fenestrata.
- Author
-
Nedashkovskaya OI, Suzuki M, Lee JS, Lee KC, Shevchenko LS, and Mikhailov VV
- Subjects
- Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Fatty Acids analysis, Flavobacteriaceae genetics, Flavobacteriaceae physiology, Locomotion, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Temperature, Flavobacteriaceae classification, Flavobacteriaceae isolation & purification, Ulva microbiology
- Abstract
Two novel aerobic, dark-orange-pigmented, Gram-negative bacterial strains, designated KMM 3531(T) and KMM 3953, were isolated from the green alga Ulva fenestrata. Analysis of the 16S rRNA gene sequences showed that the strains represented a novel lineage within the family Flavobacteriaceae. The most closely related genera with validly published names were Costertonia, Flagellimonas, Kriegella, Maribacter, Muricauda and Zobellia, with 16S rRNA gene sequence similarities of 93.3-91.8 %. Cells of strains KMM 3531(T) and KMM 3953 were rod-shaped, motile by gliding and grew at temperatures up to 49 degrees C. They produced acid from carbohydrates and possessed oxidase, catalase, beta-galactosidase and agarase activities. The predominant cellular fatty acids were iso-C(15 : 0,) iso-C(17 : 0) 3-OH, iso-C(15 : 1) G, summed feature 3 (comprising C(16 : 1)omega7c and/or iso-capital ES, Cyrillic(15 : 0) 2-OH), iso-C(17 : 1)omega9c and iso-C(15 : 0) 3-OH. The DNA G+C content was 47-49 mol%. On the basis of phenotypic and genotypic characteristics, strains KMM 3531(T) and KMM 3953 represent a novel genus and species, for which the name Pseudozobellia thermophila gen. nov., sp. nov. is proposed. The type strain is KMM 3531(T) (=DSM 19858(T)=JCM 11733(T)=KCTC 22016(T)).
- Published
- 2009
- Full Text
- View/download PDF
15. Synthesis, structure-activity relationship and evaluation of antifungal activity of tryptanthrin derivatives against drug-resistant Candida albicans.
- Author
-
Wu, Yandan, Jiang, Luyi, Liu, Ruina, Yang, Lijiao, Zou, Fei, Zhang, Tianyu, Fan, Zefei, Zhang, Tianbao, Yang, Huan, Yin, Shuyun, Wang, Ruirui, Li, Ganpeng, and Ni, Guanghui
- Abstract
With the increasing of Candida albicans infections year by year, and the widespread use of azole drugs, especially fluconazole has led to the emergence of drug resistance. Therefore, new antifungal agents are urgent needed. In this work, we synthesized a series of tryptanthrin derivatives, and all compounds were evaluated for antifungal activities against Candida albicans in vitro. The results indicated that most compounds combined with fluconazole showed good antifungal activity against drug-resistant Candida albicans. Especially, compound 5b combined with fluconazole had an excellent synergistic effect, with MIC
50 value of 0.94 μg/mL, and the FICI value of 0.005. Further mechanism study demonstrated that compound 5b significantly inhibited the hyphal growth and biofilm formation of Candida albicans. Compound 5b combined with fluconazole could be considered as a novel antifungal agent. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
16. Analysis of bacterial community of Labidochromis caeruleus reared in RAS with various feeds.
- Author
-
Chengxu Ha, Yunzhong Wang, Cuihua Yang, Peng Jin, and Yi Ren
- Subjects
CICHLIDS ,FISH microbiology ,FISH feeds ,FISH growth ,DNA sequencing - Abstract
The electric yellow cichlid (Labidochromis caeruleus), renowned for its exceptional ornamental value, enjoys widespread popularity among aquarium enthusiasts and holds significant economic value in the market. This study employed high-throughput sequencing technology to analyze the microorganisms in the circulating water of the electric yellow cichlid cultivated with six different types of feeds. Statistical results showed that the compound feed group exhibited the highest specific growth and mass gain rates. In contrast, the water flea and brine shrimp groups showed lower rankings with significant differences. 51,084 to 64,626 effective sequences were obtained and clustered into 127 to 848 operational taxonomic units (OTUs). The research findings indicate a remarkable bacterial diversity in the breeding environment of electric yellow cichlids with higher levels in the water flea and brine shrimp groups, and the lowest level in the compound feed group; the dominant bacterial phyla in all six experimental groups include Proteobacteria, Actinobacteria, Parcubacteria, and Cyanobacteria; Comparative analysis using the COGs database revealed significant differences (P<0.05) in the metabolic pathways of 24 functional microbial genes among the experimental groups. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp.
- Author
-
Nedashkovskaya OI, Kim SB, Vancanneyt M, Shin DS, Lysenko AM, Shevchenko LS, Krasokhin VB, Mikhailov VV, Swings J, and Bae KS
- Subjects
- Animals, Flavobacteriaceae chemistry, Flavobacteriaceae genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S analysis, RNA, Ribosomal, 16S genetics, Flavobacteriaceae classification, Flavobacteriaceae isolation & purification, Porifera microbiology, Seawater microbiology
- Abstract
A sponge-associated strain, KMM 7019T, was investigated in a polyphasic taxonomic study. The bacterium was strictly aerobic, heterotrophic, Gram-negative, yellow-pigmented, motile by gliding and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KMM 7019T is closely related to members of the genus Salegentibacter, namely Salegentibacter holothuriorum, Salegentibacter mishustinae and Salegentibacter salegens (97.7-98 % sequence similarities). The DNA-DNA relatedness between the strain studied and Salegentibacter species ranged from 27 to 31 %, clearly demonstrating that KMM 7019T belongs to a novel species of the genus Salegentibacter, for which the name Salegentibacter agarivorans sp. nov. is proposed. The type strain is KMM 7019T (=KCTC 12560T = LMG 23205T).
- Published
- 2006
- Full Text
- View/download PDF
18. Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter.
- Author
-
Nedashkovskaya OI, Vancanneyt M, Cleenwerck I, Snauwaert C, Kim SB, Lysenko AM, Shevchenko LS, Lee KH, Park MS, Frolova GM, Mikhailov VV, Bae KS, and Swings J
- Subjects
- Aerobiosis, Bacteroidetes genetics, Bacteroidetes isolation & purification, Japan, Molecular Sequence Data, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Sequence Homology, Nucleic Acid, Species Specificity, Bacteroidetes classification, Seawater, Ulva microbiology
- Abstract
The taxonomic position of three novel, marine, heterotrophic, aerobic, pigmented, gliding bacteria, isolated from the green alga Ulva fenestrata in the Sea of Japan, was determined. 16S rRNA gene sequence analysis revealed that the strains belong to the genus Arenibacter. The results of DNA-DNA hybridization experiments supported by phenotypic and chemotaxonomic data showed that the isolates represent a novel species of the genus Arenibacter, for which the name Arenibacter palladensis sp. nov. is proposed. The type strain is KMM 3961T (= LMG 21972T = CIP 108849T).
- Published
- 2006
- Full Text
- View/download PDF
19. Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum 'Bacteroidetes', and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003.
- Author
-
Nedashkovskaya OI, Kim SB, Suzuki M, Shevchenko LS, Lee MS, Lee KH, Park MS, Frolova GM, Oh HW, Bae KS, Park HY, and Mikhailov VV
- Subjects
- Bacteroidetes chemistry, Bacteroidetes genetics, Bacteroidetes isolation & purification, Base Composition, DNA, Bacterial analysis, DNA, Ribosomal analysis, Fatty Acids analysis, Molecular Sequence Data, RNA, Ribosomal, 16S analysis, RNA, Ribosomal, 16S genetics, Bacteroidetes classification
- Abstract
The taxonomic position of a marine, gliding, pink-pigmented, aerobic, heterotrophic and Gram-negative bacterium was established using a polyphasic approach. 16S rRNA gene sequence analysis indicated that the strain was a member of the phylum 'Bacteroidetes' in which it occupied a separate lineage. The predominant cellular fatty acids were C15 : 0 iso, C17 : 0 iso 3-OH, summed feature 3 and summed feature 4. The DNA G+C content was 48.7 mol%. Phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses strongly support the assignment of the newly isolated bacterium as a member of a novel genus and species, for which the name Pontibacter actiniarum gen. nov., sp. nov. is proposed. The type strain is KMM 6156T (=KCTC 12367T=LMG 23027T). It is also proposed that the illegitimate names Reichenbachia and Reichenbachia agariperforans are replaced with Reichenbachiella and Reichenbachiella agariperforans, respectively.
- Published
- 2005
- Full Text
- View/download PDF
20. Oceanimonas smirnovii sp. nov., a novel organism isolated from the Black Sea.
- Author
-
Ivanova EP, Onyshchenko OM, Christen R, Zhukova NV, Lysenko AM, Shevchenko LS, Buljan V, Hambly B, and Kiprianova EA
- Subjects
- Aeromonadaceae chemistry, Aeromonadaceae genetics, Aeromonadaceae isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial analysis, DNA, Ribosomal analysis, Fatty Acids analysis, Genes, rRNA, Lipids analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Ukraine, Aeromonadaceae classification, Seawater microbiology
- Abstract
A slightly creamy, melanogenic, gram-negative, aerobic bacterium was isolated from seawater sample collected in the Karadag Natural Reserve of the Eastern Crimea, the Black Sea. The novel organism was chemoorganotrophic, had no obligate requirement in NaCl, tolerated to 12% NaCl, grew between 10 and 45 degrees C, was slightly alkaliphilic, and was not able to degrade starch, gelatin, agar, and Tween 80. 16S rRNA gene sequence-based analyses of the new organism revealed that Oceanimonas doudoroffii ATCC 27123T, Oceanimonas baumanii ATCC 700832T, and Oceanisphaera litoralis DSM 15406T were the closest relatives (similarity around 97%-96%). The G + C content of the DNA of the strain 31-13T was 55.5mol%. Phosphatidylethanolamine (49.0%), phosphatidylglycerol (41.8%), and diphosphatidylglycerol (9.2%) were the predominant phospholipids. The major fatty acids were 16:0 (24.1%), 16:1omega7 (40.3%), and 18:1omega7 (29.2%). On the basis of the significant differences demonstrated in the phenotypic and chemotaxonomic characteristics, it is suggested that the bacterium be classified as a novel species; the name Oceanimonas smirnovii sp. nov. is proposed. The type strain is 31-13T (UCM B-11076T = LMG 22147T = ATCC BAA-899T).
- Published
- 2005
- Full Text
- View/download PDF
21. Erythrobacter vulgaris sp. nov., a novel organism isolated from the marine invertebrates.
- Author
-
Ivanova EP, Bowman JP, Lysenko AM, Zhukova NV, Gorshkova NM, Kuznetsova TA, Kalinovskaya NI, Shevchenko LS, and Mikhailov VV
- Subjects
- Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial analysis, DNA, Ribosomal analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Seawater, Sequence Analysis, DNA, Sphingomonadaceae chemistry, Sphingomonadaceae genetics, Sphingomonadaceae isolation & purification, Anthozoa microbiology, Sphingomonadaceae classification, Starfish microbiology
- Abstract
Four yellow-pigmented, gram-negative, chemoorganotrophic aerobic bacteria were isolated from starfish Stellaster equestris (strains 022-2-10T, 022-2-9, and 022-2-12) and soft coral (unidentified species) (strain 022-4-7) collected in the South China Sea. 16S rRNA gene sequence-based analyses of the new organisms revealed that Erythrobacter spp. were the closest relatives and shared the highest similarity of 98.7% to E. citreus, 98.5% to E. flavus, 97.9% to E. litoralis and 97.6% to E. longus. The novel organisms were tolerant to 3-6% NaCl, grew between 10 degrees C and 40 degrees C, and were not able to degrade gelatin, casein, and agar, while degraded Tween 80. Two strains (022-2-9 and 022-2-12) could weakly degrade starch. All strains produced a large pool of carotenoids and did not have Bacteriochlorophyll a. Phosphatidylethanolamine (30-36%), phosphatidylglycerol (39-46%), and phosphatidylcholine (21-27%) were the predominant phospholipids. Sphingoglycolipid was not detected. The major fatty acids were 16:0 (6-11%), 16:1omega7 (12-15%), and 18:1omega7 (46-49%). The two-hydroxy fatty acids, 13:0-2OH, 14:0-2OH, 15:0-2OH, 16:0-2OH were also present. The G + C content of the DNAs ranged from 61 to 62 mol%. The level of DNA similarity among four strains was conspecific and ranged from 94% to 98%. Even though new strains and other species of the genus had rather high level of 16S rRNA gene sequence similarities, DNA-DNA hybridization experiments showed only 33-39% of binding with the DNA of the type strains. On the basis of these results and the significant differences demonstrated in the phenotypic and chemotaxonomic characteristics, it is suggested that the new organisms be classified as a novel species; the name Erythrobacter vulgaris sp. nov. is proposed. The type strain is 022-2-10T (= KMM 3465T = CIP 107841T).
- Published
- 2005
- Full Text
- View/download PDF
22. Marinomonas pontica sp. nov., isolated from the Black Sea.
- Author
-
Ivanova EP, Onyshchenko OM, Christen R, Lysenko AM, Zhukova NV, Shevchenko LS, and Kiprianova EA
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial analysis, Genes, rRNA, Molecular Sequence Data, Oceanospirillaceae genetics, Oceanospirillaceae isolation & purification, Oceanospirillaceae physiology, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Ukraine, Oceanospirillaceae classification, Seawater microbiology
- Abstract
A Gram-negative, polarly flagellated bacterium was isolated from a sea-water sample collected from the Karadag Natural Reserve of the Eastern Crimea and characterized to clarify its taxonomic position. 16S rRNA gene sequence-based phylogenetic analysis of this novel organism revealed Marinomonas vaga, Marinomonas communis, Marinomonas mediterranea, Marinomonas primoryensis and 'Marinomonas protea' as its closest relatives (similarity 95-97 %). The G+C content of the DNA was 46.5 mol%. The organism grew between 4 and 33 degrees C, tolerated 10 % NaCl, was slightly alkaliphilic and was not able to degrade starch, gelatin, agar or Tween 80. Phosphatidylethanolamine (53.4 %) and phosphatidylglycerol (46.6 %) were the predominant phospholipids. The major fatty acids were 16 : 0 (15.5 %), 16 : 1omega7 (26.7 %) and 18 : 1omega7 (47.1 %). The phylogenetic, genetic and physiological properties of the organism placed it within a novel species, proposed as Marinomonas pontica sp. nov., the type strain of which is 46-16T (=LMG 22531T=KMM 3492T=UCM 11075T).
- Published
- 2005
- Full Text
- View/download PDF
23. Controlling production of brominated cyclic depsipeptides by Pseudoalteromonas maricaloris KMM 636T.
- Author
-
Sobolevskaya MP, Smetanina OF, Speitling M, Shevchenko LS, Dmitrenok PS, Laatsch H, Kuznetsova TA, Ivanova EP, and Elyakov GB
- Subjects
- Animals, Culture Media chemistry, Depsipeptides analysis, Depsipeptides chemistry, Depsipeptides toxicity, Female, Male, Ovum drug effects, Pigments, Biological analysis, Pigments, Biological biosynthesis, Pigments, Biological isolation & purification, Pigments, Biological toxicity, Pseudoalteromonas growth & development, Sea Urchins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spermatozoa drug effects, Bromine metabolism, Depsipeptides biosynthesis, Pseudoalteromonas metabolism
- Abstract
Aims: This study aims at evaluating the impact of the nutrient medium components on the in vitro production of the cytotoxic alterochromides., Methods and Results: The employment matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) facilitated the identification of a range of brominated cyclic depsipeptides with molecular masses of 843/845, 857/859 and 922/924/926 Da, and 936/938/940 Da produced by the marine bacterium Pseudoalteromonas maricaloris KMM 636T. The fractions of cytotoxic yellow pigments yielded after methanol extraction of P. maricaloris KMM 636T cells grown on five nutrient media were solely composed of brominated cyclic depsipeptides. Bromo-alterochromides A and B were obtained after cultivation on low nutrient media, while dibrominated derivatives were the principal components of the biosynthesis during cultivation on nutrient rich media., Conclusions: The quantity of bromo-alterochromides and their dibromo- derivates varied depending on the media composition., Significance and Impact of the Study: MALDI-TOF mass spectrometry enables to generate accurate mass analysis for the identification of peptide and its derivates which is important in controlling the production of biologically active compounds in vitro.
- Published
- 2005
- Full Text
- View/download PDF
24. Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum 'Bacteroidetes', isolated from the green alga Ulva fenestrata.
- Author
-
Nedashkovskaya OI, Kim SB, Lee DH, Lysenko AM, Shevchenko LS, Frolova GM, Mikhailov VV, Lee KH, and Bae KS
- Subjects
- Cytophagaceae chemistry, Cytophagaceae genetics, Cytophagaceae isolation & purification, DNA, Bacterial analysis, DNA, Ribosomal analysis, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Chlorophyta microbiology, Cytophagaceae classification, Seawater microbiology
- Abstract
The taxonomic position of a novel marine bacterium isolated from the green alga Ulva fenestrata collected in the Sea of Japan was established. Cells of the strain studied, designated KMM 6017T, were strictly aerobic, heterotrophic, pink-pigmented, non-motile by gliding, Gram-negative and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. 16S rRNA gene sequence analysis indicated that the strain occupied a distinct lineage within the phylum 'Bacteroidetes' and formed a cluster with [Flexibacter] tractuosus and Reichenbachia agariperforans. The G+C content of the DNA of KMM 6017T was 40.2 mol%. The major respiratory quinone was MK-7. The predominant fatty acids were i15 : 1, i15 : 0 and i17 : 0 3-OH (34.2, 24 and 7.7 %, respectively). On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacterium was assigned to the genus Roseivirga gen. nov., as Roseivirga ehrenbergii gen. nov., sp. nov. The type strain is KMM 6017T (=KCTC 12282T=LMG 22567T).
- Published
- 2005
- Full Text
- View/download PDF
25. Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [Pseudoalteromonas aurantia] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov.
- Author
-
Ivanova EP, Shevchenko LS, Sawabe T, Lysenko AM, Svetashev VI, Gorshkova NM, Satomi M, Christen R, and Mikhailov VV
- Subjects
- Animals, Australia, Base Composition, DNA, Ribosomal analysis, Fatty Acids analysis, Gammaproteobacteria genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phospholipids analysis, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Gammaproteobacteria classification, Gammaproteobacteria isolation & purification, Porifera microbiology
- Abstract
A marine, gram-negative, aerobic bacterium that produced cytotoxic, lemon-yellow, chromopeptide pigments that inhibited the development of sea urchin eggs has been isolated from the Australian sponge Fascaplysinopsis reticulata Hentschel. The cells of the organism were rod-shaped with a single polar flagellum and they required NaCl for growth (0.5-10%) with optimum growth at 1-3% NaCl. The temperature for growth was 10-37 degrees C, with optimum growth at 25-30 degrees C. Growth occurred at pH values from 6.0 to 10.0, with optimum growth at pH 6.0-8.0. Major phospholipids were phosphatidylethanolamine, phosphatidylglycerol and lyso-phosphatidylethanolamine. Of 26 fatty acids with 11-19 carbon atoms that were detected, 16:1omega7, 16:0, 17:1omega8 and 18:1omega7 were predominant. The DNA G+C content was 38.9 mol%. All of these phenotypic and chemotaxonomic characters place the organism in the genus Pseudoalteromonas (Gauthier et al, 1995). These data are consistent with the phylogenetic analyses that confirmed that strain KMM 636T is a member of the Pseudoalteromonas cluster in the gamma-subclass of the Proteobacteria. DNA-DNA hybridization experiments revealed that the levels of relatedness between the DNA of the strain studied and DNAs of type strains of the species that clustered together (on the basis of 16S rDNA sequences) and [Pseudoalteromonas aurantia] NCIMB 2033 ranged from 19 to 35%, and that the DNA-DNA homology between [P. aurantia] NCIMB 2033 and other phylogenetically and/or phenotypically similar type strains ranged from 32 to 52%. According to the polyphasic evidence presented in this study, it is proposed that strain KMM 636T (= LMG 19692T = CIP 106859T) be classified as Pseudoalteromonas maricaloris sp. nov. and [P. aurantia] NCIMB 2033 be reclassified as Pseudoalteromonas flavipulchra NCIMB 2033T (= KMM 3630T = LMG 20361T) sp. nov.
- Published
- 2002
- Full Text
- View/download PDF
26. Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas sp. KMM 634.
- Author
-
Komandrova NA, Tomshich SV, Shevchenko LS, Perepelov AV, Senchenkova SN, Shashkov AS, and Knirel YA
- Subjects
- Carbohydrate Sequence, Chromatography, Gas, Hexuronic Acids chemistry, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Sequence Data, O Antigens isolation & purification, O Antigens chemistry, Polysaccharides chemistry, Proteobacteria chemistry, Proteobacteria immunology
- Abstract
An acidic O-specific polysaccharide containing D-glucuronic acid (D-GlcA), 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid (D-GlcNAc3NAcA), 2,3-diacetamido-2,3-dideoxy-D-mannuronoyl-L-alanine (D-ManNAc3NAcA6Ala), and 2-acetamido-2,4, 6-trideoxy-4-[(S)-3-hydroxybutyramido]-D-glucose (D-QuiNAc4NAcyl) was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Pseudoalteromonas sp. KMM 634 followed by gel-permeation chromatography. The polysaccharide was cleaved selectively with a new solvolytic agent, trifluoromethanesulfonic acid, to give a disaccharide and a trisaccharide with D-GlcNAc3NAcA at the reducing end. The borohydride-reduced oligosaccharides and the initial polysaccharide were studied by GLC-MS and 1H- and 13C-NMR spectroscopy, and the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: -->3)-alpha-D-QuipNAc4Ac4NAcyl-(1-->4)-beta-D-ManpNAc3NAcA6Ala+ ++-(1-->4)-b eta-D-GlcpNAc3NAc3NAcA-(1-->4)-beta-D-GlcpA-(1-->.
- Published
- 2000
27. [Screening of marine bacteria for fucoidan hydrolases].
- Author
-
Bakunina IIu, Shevchenko LS, Nedashkovskaia OI, Shevchenko NM, Alekseeva SA, Mikhaĭlov VV, and Zviagintseva TN
- Subjects
- Animals, Eukaryota microbiology, Sea Cucumbers microbiology, Seawater, Bacteria enzymology, Hydrolases metabolism, Polysaccharides metabolism, Water Microbiology
- Abstract
Twenty-five strains of epiphytic marine bacteria isolated from the brown algae Fucus evanescens and Chorda filum and fifty-three bacteria isolated from the sea cucumber Apostichopus japonicus were screened for fucoidanases using fucoidans prepared from the brown algae F. evanescens, Laminaria cichorioides, and L. japonica. Eighteen bacterial epiphytes and thirty-eight bacterial isolates from the sea cucumber were found to contain fucoidanases, which were able to hydrolyze either all of the fucoidans studied or some of them. Bacteria of the genera Cytophaga and Alteromonas/Pseudoalteromonas exhibited the highest fucoidanase activities, which, however, did not exceed the activity of fucoidanases from the already known sources.
- Published
- 2000
28. [Structure of teichoic acids from marine microorganisms Bacillus subtilis and Bacillus licheniformis].
- Author
-
Komandrova NA, Gorshkova RP, Isakov VV, Tomshich SV, Shevchenko LS, Romanenko LA, and Nedashkovskaia OI
- Subjects
- Biopolymers chemistry, Biopolymers isolation & purification, Carbohydrate Sequence, Carbon Isotopes, Cell Wall chemistry, Glucose analysis, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Phosphoric Acids analysis, Phosphorus Isotopes, Ribitol analysis, Teichoic Acids analysis, Teichoic Acids isolation & purification, Bacillus chemistry, Bacillus subtilis chemistry, Teichoic Acids chemistry
- Abstract
Teichoic acids from the cell walls of marine bacilli Bacillus subtilis CMM (Collection of Marine Microorganisms) 234 (R-1) and B. licheniformis CMM 454 (1-1G-2) were isolated and characterized. These teichoic acids were found to have identical structures and are composed of the glucose, ribitol, and phosphoric acid residues. On the basis of 13C NMR and 31P NMR spectra of the teichoic acids and the products of their dephosphorylation, we established the following structure for the biopolymer: poly[-->5)-4-O-beta-D-glucopyranosylribitol-(1-phospho].
- Published
- 1998
29. [Quality of life of relatives of mental patients].
- Author
-
Solokhina TA, Shevchenko LS, Seĭku IuV, Rytik EG, Besedina LV, and Veshchugina TS
- Subjects
- Adult, Female, Health Status, Humans, Male, Middle Aged, Socioeconomic Factors, Surveys and Questionnaires, Family, Mental Disorders therapy, Quality of Life
- Abstract
Usage of quality of life of psychiatric patients' relatives as the parameter of estimation of the quality of psychiatric care is considered. By means of standard questionnaire elaborated by the authors as well as by means of both individual and group conversations the quality of life was analyzed in 40 relatives which lived together with psychiatric patients. Different spheres of their life and difficulties (financial, social-psychologic, production) were examined in families with such patients. The defects of psychiatric care that had direct influence on the quality of life of the psychiatric patients' relatives are outlined.
- Published
- 1998
30. [Structure of repeating unit of the O-specific polysaccharide from Yersinia mollaretii strain WS 42/89].
- Author
-
Gorshkova RP, Isakov VV, Nazarenko EL, and Shevchenko LS
- Subjects
- Heptoses analysis, Magnetic Resonance Spectroscopy, Methylation, O Antigens isolation & purification, Yersinia genetics, Yersinia enterocolitica chemistry, O Antigens chemistry, Yersinia chemistry
- Abstract
The O-specific polysaccharide was isolated from Yersinia mollaretii strain WS 42/89 and characterized. Studies of the partial hydrolysis and methylation products and the 1H and 13C NMR spectroscopy data enabled the following structure to be proposed for the repeating unit of the polysaccharide: -->2)-beta-D-Galp-(1-->3)-alpha-D-6dGulp-(1--> The same structure was ascribed to the O-specific polysaccharide from Yersinia enterocolitica serovar O:6.31. The difference between these two O-antigens from various Yersinia species is in the absence of D-glycero-D-manno-heptose residues in the structure of the lipopolysaccharide from Y. mollaretii.
- Published
- 1997
31. [A novel fungicide of the iturin group, obtained from a marine isolate of Bacillus subtilis. Isolation, physico-chemical and biochemical properties, identification].
- Author
-
Oleĭnikova GK, Shevchenko LS, Kuznetsova TA, and Mikhaĭlov VV
- Subjects
- Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Antifungal Agents chemistry, Antifungal Agents isolation & purification, Chemical Phenomena, Chemistry, Physical, Chromatography, High Pressure Liquid, Culture Media, Anti-Bacterial Agents pharmacology, Antifungal Agents pharmacology, Bacillus subtilis chemistry, Candida albicans drug effects
- Abstract
Strain KMM 457 of Bacillus subtilis was isolated from a frozen sample of the soft coral Sarcophyton sp. The samples were collected in 1989 in the South China Sea near the Vietnam Shore during an expedition on the board of R/V "Akademik Oparin". Metabolites of the isolate were investigated and it was found to produce a number of physiologically active compounds. One of them designated as OGA showed fungicidal activity. By the physicochemical properties it was referred to the group of iturins. The comparison with the described antibiotics of the iturin group suggested that it was a new representative of the iturin group.
- Published
- 1995
32. [The economic aspects of the activities of a mental health service].
- Author
-
Shevchenko LS, Solokhina TA, Seĭku IuV, Rytik EG, and Rotshteĭn VG
- Subjects
- Health Expenditures, Hospital Charges, Hospital Costs, Hospitals, Psychiatric economics, Humans, Mental Disorders economics, Moscow, USSR, Mental Health Services economics
- Abstract
Integral economic loss of the society, connected with mental disease, were calculated by the author and presented. The author determined "direct" and "connected" state costs for psychiatric patients, and the volume of non produced national income (indirect costs) because psychiatric patients with temporal or constant disability are excluded from producing process. Financial state of psychiatric hospitals in Moscow was analysed and reserves for improvement of money spending were found out. Calculations of expenses of admission of patients with different diseases to Psychiatric Hospital N 1 by P.P. Kashenko in 1988 were performed. Principles of financing of psychiatric hospitals on the basis of clinical-statistic groups are discussed. The method of determination of prices for medical and diagnostic services to patients is presented.
- Published
- 1995
33. [The socioeconomic problems of a mental health service].
- Author
-
Iastrebov VS, Shevchenko LS, Solokhina TA, Rotshteĭn VG, and Evtushenko VIa
- Subjects
- Delivery of Health Care economics, Delivery of Health Care statistics & numerical data, Delivery of Health Care trends, Financing, Government economics, Financing, Government statistics & numerical data, Financing, Government trends, Humans, Insurance, Psychiatric economics, Insurance, Psychiatric statistics & numerical data, Insurance, Psychiatric trends, Mental Health Services statistics & numerical data, Mental Health Services trends, Russia, Socioeconomic Factors, USSR, Mental Health Services economics
- Abstract
The state and prospects of the development of mental health service are discussed. Special attention is drawn to the economic aspects of mental health service and financial problems, in particular. The principles of creation and utilization of financial resources for psychiatric facilities in conditions of medical insurance are given. Social and economic aspects of mental diseases and their cost for the society are considered. The conclusion is made on the priority of psychiatric care programmes among other programmes of health care, that must be solved on the state level.
- Published
- 1993
34. [A method for integral assessment of the effect of the industrial environment on miners' bodies].
- Author
-
Goretskiĭ OS, Maksimovich VA, Shevchenko LS, and Mal'tsev VA
- Subjects
- Adult, Humans, Male, Mathematics, Coal Mining, Models, Biological, Occupational Exposure
- Published
- 1991
35. [Structure of the O-specific polysaccharide chain of the Yersinia pseudotuberculosis lipopolysaccharide (serovar II C)].
- Author
-
Gorshkova RP, Isakov VV, Shevchenko LS, and Ovodov IuS
- Subjects
- Carbohydrate Conformation, Hydrogen-Ion Concentration, Hydrolysis, Magnetic Resonance Spectroscopy, Oxidation-Reduction, Lipopolysaccharides chemistry, Polysaccharides, Bacterial chemistry, Yersinia pseudotuberculosis metabolism
- Abstract
An O-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from Yersinia pseudotuberculosis serovar IIc and shown to consist of abequose, D-mannose and 2-acetamido-2-deoxy-D-galactose residues in the ratio 0.8:3:1. From the results of acid hydrolysis, 13C NMR, methylation and periodate oxidation studies the structure of the repeating unit of the O-specific polysaccharide is deduced as follows: (formula; see text)
- Published
- 1991
36. [The financing characteristics of psychiatric hospitals based on the principle of diagnosis-related (clinico-statistical) groups (a review)].
- Author
-
Rytik EG, Shevchenko LS, Solokhina TA, and Seĭku IuV
- Subjects
- Bed Occupancy economics, Cost Control economics, Prospective Payment System economics, USSR, United States, Diagnosis-Related Groups economics, Financing, Organized economics, Hospitals, Psychiatric economics
- Published
- 1991
37. New investigation of encoding secondary metabolites gene by genome mining of a marine bacterium, Pseudoalteromonas viridis BBR56.
- Author
-
Handayani, Desy Putri, Isnansetyo, Alim, and Istiqomah, Indah
- Subjects
NUCLEIC acid hybridization ,METABOLITES ,OCEAN mining ,MARINE bacteria ,GENOMES ,TRANSFER RNA ,POLYKETIDE synthases - Abstract
Pseudoalteromonas viridis strain BBR56 was isolated from seawater at Dutungan Island, South Sulawesi, Indonesia. Bacterial DNA was isolated using Promega Genomic DNA TM050. DNA purity and quantity were assessed using NanoDrop spectrophotometers and Qubit fluorometers. The DNA library and sequencing were prepared using Oxford Nanopore Technology GridION MinKNOW 20.06.9 with long read, direct, and comprehensive analysis. High accuracy base calling was assessed with Guppy version 4.0.11. Filtlong and NanoPlot were used for filtering and visualizing the FASTQ data. Flye (2.8.1) was used for de novo assembly analysis. Variant calls and consensus sequences were created using Medaka. The annotation of the genome was elaborated by DFAST. The assembled genome and annotation were tested using Busco and CheckM. Herein, we found that the highest similarity of the BBR56 isolate was 98.37% with the 16 S rRNA gene sequence of P. viridis G-1387. The genome size was 5.5 Mb and included chromosome 1 (4.2 Mbp) and chromosome 2 (1.3 Mbp), which encoded 61 pseudogenes, 4 noncoding RNAs, 113 tRNAs, 31 rRNAs, 4,505 coding DNA sequences, 4 clustered regularly interspaced short palindromic repeats, 4,444 coding genes, and a GC content of 49.5%. The sequence of the whole genome of P. viridis BBR56 was uploaded to GenBank under the accession numbers CP072425–CP072426, biosample number SAMN18435505, and bioproject number PRJNA716373. The sequence read archive (SRR14179986) was successfully obtained from NCBI for BBR56 raw sequencing reads. Digital DNA–DNA hybridization results showed that the genome of BBR56 had the potential to be a new species because no other bacterial genomes were similar to the sample. Biosynthetic gene clusters (BGCs) were assessed using BAGEL4 and the antiSMASH bacterial version. The genome harbored diverse BGCs, including genes that encoded polyketide synthase, nonribosomal peptide synthase, RiPP-like, NRP-metallophore, hydrogen cyanide, betalactone, thioamide-NRP, Lant class I, sactipeptide, and prodigiosin. Thus, BBR56 has considerable potential for further exploration regarding the use of its secondary metabolite products in the human and fisheries sectors. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. Structural tailoring of fucoidan backbones for maximizing their benefits: Enzymatic, chemical, and physical strategies.
- Author
-
Zayed, Ahmed, Cao, Hang Thi Thuy, Trang, Vo Thi Dieu, and Ulber, Roland
- Abstract
Fucoidans are a heterogenous class of sulfated, fucose-rich, and high molecular weight polysaccharides derived mainly from brown seaweeds. They have attracted particular interest in different scientific and industrial fields. Nevertheless, their applications are affected by various chemical properties, including molecular weight, sulfation pattern, and degree of sulphation. For instance, most of the reported bioactivities (e.g., immunomodulatory and cytotoxic activities) have been enhanced with more sulfated and low molecular weight fractions, in contrast to the anticoagulant effect. Additionally, high molecular weight fucoidans are unsuitable for nanomedicine formulation, oral absorption, and distribution to various body organs. Hence, different strategies have been developed as a subsequent stage of processing to modify the native fucoidans in order to produce more bioactive and efficiently formulated products. The current article highlights the various enzymatic (e.g., fucoidanase and fucoidan sulfatase), chemical, and other strategies used for structural modifications of native fucoidans accompanied by varied bioactivities. Interestingly, the enzymatic method has proved to be more specific and selective retaining the native sulfated chemical backbone efficiently. Hence, this review may help in tayloring new fucoidan derivatives for targeted homogenous production and maximized applications of fucoidans in the medical and pharmaceutical fields. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
39. [Results and objectives of developing research on occupational hygiene in the coal mining industry].
- Author
-
Kobets GP and Shevchenko LS
- Subjects
- Ukraine, Anthracosilicosis prevention & control, Coal Mining standards, Occupational Medicine standards
- Published
- 1988
40. [Adaptation of the human body to muscular work under warm and humid microclimate conditions].
- Author
-
Maksimovich VA, Shevchenko LS, Kudinova TV, and Ostapenko VI
- Subjects
- Adult, Humans, Male, Mathematics, Adaptation, Physiological, Climate, Heart, Humidity, Physical Exertion
- Published
- 1977
41. [Characteristics of body reactions of mine rescue workers to the combined effects of hyperthermia and hyperoxia].
- Author
-
Maksimovich VA, Shevchenko LS, Goretskiĭ OS, and Mironiuk DIa
- Subjects
- Adult, Body Temperature Regulation, Humans, Middle Aged, Hot Temperature, Mining, Oxygen pharmacology, Physical Endurance
- Published
- 1987
42. [Ecological and epidemiological problems of the natural type of rabies].
- Author
-
Shcherbak IuN, Riaboshapka AP, and Shevchenko LS
- Subjects
- Animal Diseases epidemiology, Animals, Animals, Wild, Bites and Stings microbiology, Cats, Disease Reservoirs veterinary, Dogs, Ecology, Foxes, Humans, Mice, Rabies transmission, Rabies veterinary, Rabies virus pathogenicity, Rats, Rodentia, Ukraine, Virulence, Rabies epidemiology
- Published
- 1978
43. [Predicting human physical work capacity at high environmental temperatures].
- Author
-
Goretskiĭ OS, Maksimovich VA, Shevchenko LS, and Mioniuk DIa
- Subjects
- Adult, Humans, Male, Hot Temperature, Physical Endurance
- Published
- 1985
44. Prediction of human physical working capacity in high ambient temperatures.
- Author
-
Goretskii OS, Maksimovich VA, Shevchenko LS, and Mironyuk DYa
- Subjects
- Adult, Erythrocytes physiology, Hemolysis, Hot Temperature, Humans, Hyperbaric Oxygenation, Male, Mining, Rest, Work Capacity Evaluation, Body Temperature, Physical Exertion
- Published
- 1985
45. [On the toxicity of aerosol solutions of calcinated water and sulfonol].
- Author
-
Reshetiuk AL and Shevchenko LS
- Subjects
- Animals, Environmental Exposure, Occupational Medicine, Aerosols, Calcium toxicity, Sulfones toxicity, Surface-Active Agents toxicity
- Published
- 1968
46. [Toxicologic assessment of surface-active agents (SAA) used in suppressing coal dust in mines].
- Author
-
Reshetiuk AL and Shevchenko LS
- Subjects
- Animals, Ions, Mice, Rabbits, Rats, Coal Mining, Dust prevention & control, Surface-Active Agents toxicity
- Published
- 1968
47. Studying the discipline of free choice «Ultrasound Diagnostics in Obstetrics and Gynaecology».
- Author
-
Ancheva, I. A., Gladchuk, I. Z., Mokriienko, E. M., Movlyanova, N. V., and Lazor, N. V.
- Subjects
MOTIVATION (Psychology) ,GYNECOLOGY ,DIGITAL health ,CRITICAL thinking ,OBSTETRICS ,ABILITY ,TRAINING ,HEALTH literacy ,HEALTH ,INFORMATION resources ,TECHNOLOGY - Abstract
Today, in the education system, an important opportunity for higher education applicants is the right to form an individual educational trajectory according to the relevant qualification level. Higher education seekers have received not only academic mobility but also the opportunity to study disciplines to consciously choose their specialty and study it more deeply. The skills of ultrasound diagnostics increase the professional level of a doctor of any specialization and open wide diagnostic possibilities. The article reveals the peculiarity of studying one of the subjects of free choice by medical higher education applicants on the basis of Odesa National Medical University – “Ultrasound Diagnostics in Obstetrics and Gynaecology”. The adaptation of the educational process in a mixed format with the possibility of using digital learning technologies and mastering professional skills on ultrasound machines of different classes is described. In the course of studying the discipline, the necessary basic digital skills of using an ultrasound scanner and knowledge of the main visualization modes, adjustment principles, as well as sets of functional capabilities inherent in all modern devices are formed. The method of presentation of theoretical information and the use of case scenarios during the discussion of clinical tasks with mandatory visualization of ultrasound video recordings is described. The applicants’ digital skills are directly related to the course being studied and are necessary to master the proposed professional field. The possibility of learning using the latest medical equipment in combination with the use of digital learning tools and video resources contributes to the acquisition of professional competence and the formation of responsibility and interest of students. In the process of training manual scanning skills, the doctor’s thinking and the ability to analyze and interpret the received ultrasound data are formed. Practical classes help to discuss the most interesting issues for this specific group of higher education applicants in a comfortable and trusting environment. The use of a combined approach in the presentation of the material, the author’s style and the format of the transfer of professional skills by experienced teachers who are engaged in constant practical activities motivate, improve the training process and give a high result. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
48. Whole-genome sequencing of Pseudoalteromonas piscicida 2515 revealed its antibacterial potency against Vibrio anguillarum: a preliminary invitro study.
- Author
-
Wang F, Ghonimy A, and Wang X
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Penaeidae microbiology, Phylogeny, Base Composition, Pseudoalteromonas genetics, Vibrio genetics, Vibrio drug effects, Whole Genome Sequencing, Genome, Bacterial, Probiotics
- Abstract
Pseudoalteromonas piscicida 2515, isolated from Litopenaeus vannamei culture water, is a potential marine probiotic with broad anti-Vibrio properties. However, genomic information on P. piscicida 2515 is scarce. In this study, the general genomic characteristics and probiotic properties of the P. piscicida 2515 strain were analysed. In addition, we determined the antibacterial mechanism of this bacterial strain by scanning electron microscopy (SEM). The results indicated that the whole-genome sequence of P. piscicida 2515 contained one chromosome and one plasmid, including a total length of 5,541,406 bp with a G + C content of 43.24%, and 4679 protein-coding genes were predicted. Various adhesion-related genes, amino acid and vitamin metabolism and biosynthesis genes, and stress-responsive genes were found with genome mining tools. The presence of genes encoding chitin, bromocyclic peptides, lantibiotics, and sactipeptides showed the strong antibacterial activity of the P. piscicida 2515 strain. Moreover, in coculture with Vibrio anguillarum, P. piscicida 2515 displayed vesicle/pilus-like structures located on its surface that possibly participated in its bactericidal activity, representing an antibacterial mechanism. Additionally, 16 haemolytic genes and 3 antibiotic resistance genes, including tetracycline, fluoroquinolone, and carbapenem were annotated, but virulence genes encoding enterotoxin FM (entFM), cereulide (ces), and cytotoxin K were not detected. Further tests should be conducted to confirm the safety characteristics of P. piscicida 2515, including long-term toxicology tests, ecotoxicological assessment, and antibiotic resistance transfer risk assessment. Our results here revealed a new understanding of the probiotic properties and antibacterial mechanism of P. piscicida 2515, in addition to theoretical information for its application in aquaculture., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
- Published
- 2024
- Full Text
- View/download PDF
49. Marinigracilibium pacificum gen. nov., sp. nov., a starch-degrading bacterium isolated from tropical western Pacific.
- Author
-
Jiang, Zhichen, Li, Yangjie, and Zhang, Dechao
- Abstract
The Gram-stain-negative, golden-yellow-colored, non-spore-forming, strictly aerobic, slender rod-shaped bacterial strain, designated KN852
T , was isolated from a seamount in the tropical western Pacific. The predominant respiratory quinone was MK-7 and the polar lipid profiles contained phosphatidylethanolamine, one unidentified phospholipid and six unidentified polar lipids. The predominant cellular fatty acids were iso-C15:0 , summed feature 3(C16:1 ω7c and/or iso-C15:0 2OH), iso-C17:0 3OH and iso-C15:1 G. Phylogenetic analyses of 16S rRNA gene sequence revealed that strain KN852T was affiliated with the family Flammeovirgaceae of the phylum Bacteroidota and formed a distinct lineage. The genomic DNA G + C content of strain KN852T was 34.8%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence presented, strain KN852T represents a novel species of a novel genus of the family Flammeovirgaceae, for which the name Marinigracilibium pacificum gen. nov., sp. nov. is proposed. The type strain is KN852T (= CGMCC 1.17119T = KCTC 72433T ). [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
50. Investigation of the enzymes required for the biosynthesis of 2,3‐diacetamido‐2,3‐dideoxy‐d‐glucuronic acid in Psychrobacter cryohalolentisK5T.
- Author
-
Hofmeister, Daniel L., Seltzner, Chase A., Bockhaus, Nicholas J., Thoden, James B., and Holden, Hazel M.
- Abstract
Psychrobacter cryohalolentis K5T is a Gram‐negative bacterium first isolated from Siberian permafrost in 2006. It has a complex O‐antigen containing l‐rhamnose, d‐galactose, two diacetamido‐sugars, and one triacetamido‐sugar. The biosynthetic pathway for one of the diacetamido‐sugars, namely 2,3‐diacetamido‐2,3‐dideoxy‐d‐glucuronic acid, is presently unknown. Utilizing the published genome sequence of P. cryohalolentis K5T, we hypothesized that the genes designated Pcryo_0613, Pcryo_0614, Pcryo_0616, and Pcryo_0615 encode for a uridine dinucleotide (UDP)‐N‐acetyl‐d‐glucosamine 6‐dehydrogenase, an nicotinamide adenine dinucleotide (oxidized) (NAD+)‐dependent dehydrogenase, a pyridoxal 5′‐phosphate (PLP)‐dependent aminotransferase, and an N‐acetyltransferase, respectively, activities of which would be required for the biosynthesis of this unusual carbohydrate. Here we present the cloning, overexpression, and purification of these hypothetical proteins. Kinetic data on the enzymes encoded by Pcryo_0613, Pcryo_0614, and Pcryo_0615 confirmed their postulated biochemical activities. In addition, the high‐resolution X‐ray structures of both the internal and external aldimine forms of the aminotransferase were determined to 1.25 and 1.0 Å, respectively. Finally, the three‐dimensional architecture of the N‐acetyltransferase in complex with its substrate and coenzyme A was solved to 1.8 Å resolution. Strikingly, the N‐acetyltransferase was shown to adopt a new motif for UDP‐sugar binding. The data presented herein provide additional insight into sugar biosynthesis in Gram‐negative bacteria. PDB Code(s): 8E62, 8E75 and 8E77 [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.