Search

Your search keyword '"Piu, Pietro"' showing total 180 results
Start Over "Piu, Pietro"
180 results on '"Piu, Pietro"'

4. FixaTons: A collection of Human Fixations Datasets and Metrics for Scanpath Similarity

Author

Zanca, Dario, Serchi, Valeria, Piu, Pietro, Rosini, Francesca, and Rufa, Alessandra

Subjects
Computer Science - Artificial Intelligence, Computer Science - Computer Vision and Pattern Recognition
Abstract

In the last three decades, human visual attention has been a topic of great interest in various disciplines. In computer vision, many models have been proposed to predict the distribution of human fixations on a visual stimulus. Recently, thanks to the creation of large collections of data, machine learning algorithms have obtained state-of-the-art performance on the task of saliency map estimation. On the other hand, computational models of scanpath are much less studied. Works are often only descriptive or task specific. This is due to the fact that the scanpath is harder to model because it must include the description of a dynamic. General purpose computational models are present in the literature, but are then evaluated in tasks of saliency prediction, losing therefore information about the dynamics and the behaviour. In addition, two technical reasons have limited the research. The first reason is the lack of robust and uniformly used set of metrics to compare the similarity between scanpath. The second reason is the lack of sufficiently large and varied scanpath datasets. In this report we want to help in both directions. We present FixaTons, a large collection of datasets human scanpaths (temporally ordered sequences of fixations) and saliency maps. It comes along with a software library for easy data usage, statistics calculation and implementation of metrics for scanpath and saliency prediction evaluation.

Published
2018

6. Development and characterization of high-throughput serological assays to measure magnitude and functional immune response against S. Paratyphi A in human samples.

Author

Carducci, Martina, Massai, Luisa, Lari, Elisa, Semplici, Bianca, Grappi, Silvia, Maria, Noshi, Jones, Elizabeth, Conti, Valentino, Piu, Pietro, Scorza, Francesco Berlanda, Iturriza-Gómara, Miren, Montomoli, Emanuele, Pollard, Andrew J., Rondini, Simona, and Rossi, Omar

Abstract

Typhoid and Paratyphoid fever cause a global health burden, especially for the children of Southern Asia. The impact of the disease is further exacerbated by the dramatic increase of antimicrobial resistance. While vaccines against Salmonella Typhi have been developed and successfully introduced, an effective vaccine targeting S. Paratyphi A is still lacking. Several efforts are currently ongoing to develop vaccines targeting both S. Typhi and S. Paratyphi A. In order to analyze the immune response induced by vaccination and in sero-epidemiological studies, easy to perform and high throughput immunoassays are needed. Here we present the setup and characterization of a customized ELISA assay and of a luminescent-based serum bactericidal assay (L-SBA) to measure the quantity of S. Paratyphi O antigen specific antibodies and their functional activity against S. Paratyphi A. Robust quality control criteria have been put in place both for ELISA and SBA and assays have been fully characterized in terms of quantitation limit, limit of blanks, specificity, linearity and precision. Assays are being employed to analyze samples from clinical trials, enabling the assessment of immunogenicity during clinical vaccine development. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Qualification of an enzyme-linked immunosorbent assay for quantification of anti-Vi IgG in human sera.

Author

Carducci, Martina, Massai, Luisa, Lari, Elisa, Semplici, Bianca, Aruta, Maria Grazia, De Simone, Daniele, Piu, Pietro, Montomoli, Emanuele, Berlanda Scorza, Francesco, Grappi, Silvia, Iturriza-Gómara, Miren, Canals, Rocio, Rondini, Simona, and Rossi, Omar

Subjects
TYPHOID fever, ENZYME-linked immunosorbent assay, SALMONELLA typhi, POLYSACCHARIDES, VACCINE effectiveness
Abstract

Effective vaccines against Salmonella Typhi, targeting the Vi capsular polysaccharide, have been developed and are being introduced into routine immunization in endemic countries. Vi conjugated vaccines are also being tested in new multi-component vaccine formulations. Simple, high-throughput and cost-effective assays to quantify Vi-specific IgG in clinical sera are needed. In this study we present the development and qualification of a new anti-Vi ELISA with continuous readout, which expresses results as ELISA Unit/mL (EU/mL). We have qualified the assay in terms of precision, linearity and specificity, demonstrating performance in line with a commercially available anti-Vi ELISA. We have also calibrated the assay against the 16/138 anti-Vi international standard and established conversion factor between EU/mL and international units/mL, to allow comparability of results across studies. In summary, this newassaymet all the suitability criteria and is being used to evaluate anti-Vi responses in clinical studies. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

8. A validate and standardized pseudotyped microneutralization assay as a safe and powerful tool to measure LASSA neutralising antibodies for vaccine development and comparison

Author

Antonelli, Roberta, primary, Forconi, Vittoria, additional, Molesti, Eleonora, additional, Semplici, Claudia, additional, Piu, Pietro, additional, Altamura, Maria, additional, Dapporto, Francesca, additional, Temperton, Nigel, additional, Montomoli, Emanuele, additional, and Manenti, Alessandro, additional

Published
2024
Full Text
View/download PDF

9. Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells

Author

Dapporto, Francesca, primary, De Tommaso, Domiziana, additional, Marrocco, Camilla, additional, Piu, Pietro, additional, Semplici, Claudia, additional, Fantoni, Giulia, additional, Ferrigno, Ilaria, additional, Piccini, Giulia, additional, Monti, Martina, additional, Vanni, Francesca, additional, Razzano, Ilaria, additional, Manini, Ilaria, additional, Montomoli, Emanuele, additional, and Manenti, Alessandro, additional

Published
2023
Full Text
View/download PDF

10. The Role of e-Commerce in the Success of Low-cost Carriers

Author

Ginanneschi Marco and Piu Pietro

Subjects
e-commerce, e-consumers, low-cost carrier, Ryanair, Granger-causality, L10, L93, C53, C63, Business, HF5001-6182
Abstract

This research aims to answer the question if e-commerce favoured in a special way the growth of low-cost carriers within the civil aviation market. After defining low-cost and traditional carriers’ business models, data on transported passengers were collected for three countries (Italy, Germany and Spain) and confronted with the number of e-consumers. Despite a significant correlation in all the three markets, only in Italy our hypothesis has been supported by Granger causality, and the regression analysis allows to forecast a future characterized by a growing dominance of LCCs. Although the definition of an econometric model will require further studies, the distinctive features of the Italian market might represent a starting point for future research on the complex relationship between e-commerce and air transport.

Published
2018
Full Text
View/download PDF

12. Establishment and validation of a high‐throughput micro‐neutralization assay for respiratory syncytial virus (subtypes A and B)

Author

Bonifazi, Carolina, primary, Trombetta, Claudia Maria, additional, Barneschi, Irene, additional, Latanza, Simona, additional, Leopoldi, Sara, additional, Benincasa, Linda, additional, Leonardi, Margherita, additional, Semplici, Claudia, additional, Piu, Pietro, additional, Marchi, Serena, additional, Montomoli, Emanuele, additional, and Manenti, Alessandro, additional

Published
2023
Full Text
View/download PDF

14. Characterization of Enzyme-Linked Immunosorbent Assay (ELISA) for Quantification of Antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-Antigens in Human Sera.

Author

Aruta, Maria Grazia, Lari, Elisa, De Simone, Daniele, Semplici, Bianca, Semplici, Claudia, Dale, Helen, Chirwa, Esmelda, Kadwala, Innocent, Mbewe, Maurice, Banda, Happy, Iturriza-Gomara, Miren, Gordon, Melita, Nyirenda, Tonney, Piu, Pietro, Pizza, Mariagrazia, Berlanda Scorza, Francesco, Grappi, Silvia, Canals, Rocío, and Rossi, Omar

Subjects
SALMONELLA typhimurium, ENZYME-linked immunosorbent assay, SALMONELLA enteritidis, IMMUNOGLOBULINS, VACCINE trials, VACCINE immunogenicity
Abstract

Nontyphoidal Salmonella (NTS) is a leading cause of morbidity and mortality caused by enteric pathogens worldwide in both children and adults, and vaccines are not yet available. The measurement of antigen-specific antibodies in the sera of vaccinated or convalescent individuals is crucial to understand the incidence of disease and the immunogenicity of vaccine candidates. A solid and standardized assay used to determine the level of specific anti-antigens IgG is therefore of paramount importance. In this work, we presented the characterization of a customized enzyme-linked immunosorbent assay (ELISA) with continuous readouts and a standardized definition of EU/mL. We assessed various performance parameters: standard curve accuracy, dilutional linearity, intermediate precision, specificity, limits of blanks, and quantification. The simplicity of the assay, its high sensitivity and specificity coupled with its low cost and the use of basic consumables and instruments without the need of high automation makes it suitable for transfer and application to different laboratories, including resource-limiting settings where the disease is endemic. This ELISA is, therefore, fit for purpose to be used for quantification of antibodies against Salmonella Typhimurium and Salmonella Enteritidis O-antigens in human samples, both for vaccine clinical trials and large sero-epidemiological studies. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

16. The neutralizing response to SARS-CoV-2 Omicron variants BA.1 and BA.2 in COVID-19 patients and homologous and heterologous vaccinees

Author

Dapporto, Francesca, primary, Leonardi, Margherita, additional, Trombetta, Claudia Maria, additional, Semplici, Claudia, additional, Piu, Pietro, additional, Piccini, Giulia, additional, Benincasa, Linda, additional, Marchi, Serena, additional, Andreano, Emanuele, additional, Lovreglio, Piero, additional, Buonvino, Nicola, additional, Decaro, Nicola, additional, Stufano, Angela, additional, Lorusso, Eleonora, additional, Bombardieri, Emilio, additional, Ruello, Antonella, additional, Viviani, Simonetta, additional, Rappuoli, Rino, additional, Molesti, Eleonora, additional, Manenti, Alessandro, additional, and Montomoli, Emanuele, additional

Published
2022
Full Text
View/download PDF

18. Antibody Avidity and Neutralizing Response against SARS-CoV-2 Omicron Variant after Infection or Vaccination

Author

Dapporto, Francesca, primary, Marchi, Serena, additional, Leonardi, Margherita, additional, Piu, Pietro, additional, Lovreglio, Piero, additional, Decaro, Nicola, additional, Buonvino, Nicola, additional, Stufano, Angela, additional, Lorusso, Eleonora, additional, Bombardieri, Emilio, additional, Ruello, Antonella, additional, Viviani, Simonetta, additional, Molesti, Eleonora, additional, Trombetta, Claudia Maria, additional, Manenti, Alessandro, additional, and Montomoli, Emanuele, additional

Published
2022
Full Text
View/download PDF

20. Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects

Author

Manenti, Alessandro, primary, Gianchecchi, Elena, additional, Dapporto, Francesca, additional, Leonardi, Margherita, additional, Cantaloni, Paolo, additional, Fattorini, Filippo, additional, Piu, Pietro, additional, Bollati, Valentina, additional, Pastorino, Ugo, additional, Apolone, Giovanni, additional, Sozzi, Gabriella, additional, and Montomoli, Emanuele, additional

Published
2022
Full Text
View/download PDF

22. Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies

Author

Torelli, Alessandro, primary, Gianchecchi, Elena, additional, Monti, Martina, additional, Piu, Pietro, additional, Barneschi, Irene, additional, Bonifazi, Carolina, additional, Coluccio, Rosa, additional, Ganfini, Luisa, additional, La Magra, Luciano Michele, additional, Marconi, Silvia, additional, Marzucchi, Ginevra, additional, Pace, Ramona, additional, Palladino, Laura, additional, Biagi, Bernardo, additional, and Montomoli, Emanuele, additional

Published
2021
Full Text
View/download PDF

23. Characterisation of SARS-CoV-2 Lentiviral Pseudotypes and Correlation between Pseudotype-Based Neutralisation Assays and Live Virus-Based Micro Neutralisation Assays

Author

Hyseni, Inesa, Molesti, Eleonora, Benincasa, Linda, Piu, Pietro, Casa, Elisa, Temperton, Nigel J., Manenti, Alessandro, Montomoli, Emanuele, Hyseni, Inesa, Molesti, Eleonora, Benincasa, Linda, Piu, Pietro, Casa, Elisa, Temperton, Nigel J., Manenti, Alessandro, and Montomoli, Emanuele

Abstract

The recent outbreak of a novel Coronavirus (SARS-CoV-2) and its rapid spread across the continents has generated an urgent need for assays to detect the neutralising activity of human sera or human monoclonal antibodies against SARS-CoV-2 spike protein and to evaluate the serological immunity in humans. Since the accessibility of live virus microneutralisation (MN) assays with SARS-CoV-2 is limited and requires enhanced bio-containment, the approach based on "pseudotyping" can be considered a useful complement to other serological assays. After fully characterising lentiviral pseudotypes bearing the SARS-CoV-2 spike protein, we employed them in pseudotype-based neutralisation assays in order to profile the neutralising activity of human serum samples from an Italian sero-epidemiological study. The results obtained with pseudotype-based neutralisation assays mirrored those obtained when the same panel of sera was tested against the wild type virus, showing an evident convergence of the pseudotype-based neutralisation and MN results. The overall results lead to the conclusion that the pseudotype-based neutralisation assay is a valid alternative to using the wild-type strain, and although this system needs to be optimised and standardised, it can not only complement the classical serological methods, but also allows serological assessments to be made when other methods cannot be employed, especially in a human pandemic context.

Published
2020

24. Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples

Author

Mazzini, Livia, primary, Martinuzzi, Donata, additional, Hyseni, Inesa, additional, Benincasa, Linda, additional, Molesti, Eleonora, additional, Casa, Elisa, additional, Lapini, Giulia, additional, Piu, Pietro, additional, Trombetta, Claudia Maria, additional, Marchi, Serena, additional, Razzano, Ilaria, additional, Manenti, Alessandro, additional, and Montomoli, Emanuele, additional

Published
2021
Full Text
View/download PDF

25. Electromechanical delay by speckle-tracking echocardiography: A novel tool to distinguish between Brugada syndrome and isolated right bundle branch block

Author

D'Ascenzi, Flavio, primary, Sanz-De La Garza, María, additional, Anselmi, Francesca, additional, Nunno, Loredana, additional, Arbelo, Elena, additional, Jordà, Paloma, additional, Marzotti, Tommaso, additional, Aprile, Federica, additional, Piu, Pietro, additional, Natali, Benedetta Maria, additional, Brugada, Josep, additional, Sitges, Marta, additional, and Mondillo, Sergio, additional

Published
2020
Full Text
View/download PDF

27. Serologically-Based Evaluation of Cross-Protection Antibody Responses among Different A(H1N1) Influenza Strains

Author

Marchi, Serena, primary, Manini, Ilaria, additional, Kistner, Otfried, additional, Piu, Pietro, additional, Remarque, Edmond J., additional, Manenti, Alessandro, additional, Biuso, Fabrizio, additional, Carli, Tommaso, additional, Lazzeri, Giacomo, additional, Montomoli, Emanuele, additional, and Trombetta, Claudia Maria, additional

Published
2020
Full Text
View/download PDF

30. Influenza D Virus: A Potential Threat for Humans?

Author

Trombetta, Claudia Maria, primary, Marchi, Serena, additional, Manini, Ilaria, additional, Kistner, Otfried, additional, Li, Feng, additional, Piu, Pietro, additional, Manenti, Alessandro, additional, Biuso, Fabrizio, additional, Sreenivasan, Chithra, additional, Druce, Julian, additional, and Montomoli, Emanuele, additional

Published
2020
Full Text
View/download PDF

31. Two‐point‐NGS analysis of cancer genes in cell‐free DNA of metastatic cancer patients

Author

Palmieri, Maria, primary, Baldassarri, Margherita, additional, Fava, Francesca, additional, Fabbiani, Alessandra, additional, Gelli, Elisa, additional, Tita, Rossella, additional, Torre, Pamela, additional, Petrioli, Roberto, additional, Hadijstilianou, Theodora, additional, Galimberti, Daniela, additional, Cinotti, Elisa, additional, Bengala, Carmelo, additional, Mandalà, Marco, additional, Piu, Pietro, additional, Miano, Salvatora Tindara, additional, Martellucci, Ignazio, additional, Vannini, Agnese, additional, Pinto, Anna Maria, additional, Mencarelli, Maria Antonietta, additional, Marsili, Stefania, additional, Renieri, Alessandra, additional, and Frullanti, Elisa, additional

Published
2020
Full Text
View/download PDF

33. Reference values of left atrial size and function according to age : should we redefine the normal upper limits?

Author

D'Ascenzi, Flavio, Piu, Pietro, Capone, Valentina, Sciaccaluga, Carlotta, Solari, Marco, Mondillo, Sergio, Henein, Michael, D'Ascenzi, Flavio, Piu, Pietro, Capone, Valentina, Sciaccaluga, Carlotta, Solari, Marco, Mondillo, Sergio, and Henein, Michael

Abstract

Different cut-offs have been proposed for left atrial (LA) size. Furthermore, conflicting results have been reported about the influence of age on LA size and data on the impact of age on LA myocardial function are scanty. The aim of this study was to derive references values for LA size and function in healthy subjects and to evaluate the impact of age. We conducted a systematic literature search of MEDLINE database. We included only studies evaluating healthy subjects, with age ranged between 18 and 80years. Parameters were compared among four age groups, <30, 30-45, >45-60, >60years. Three hundred twenty-six studies met the inclusion criteria and the final population consisted of 62,821 subjects. LA volume index (LAVi) did not differ among different age groups (p=0.21). The normal upper limit of LAVi was 24mL/m(2). LA reservoir function, measured by strain, did not differ among age groups (38 +/- 3%, 32-43%; p=0.74). Left ventricular (LV) size and function were not different among groups, except LV mass index. A decrease in E/A ratio and an increase in E/e ratio were found with advancing age (p<0.0001 and p=0.001, respectively). In healthy subjects the normal upper limit of LAVi was lower than that recommended and is not influenced by advancing age. Furthermore, also LA function measured by strain was not affected by age. The current reference values of LAVi should be used with caution when applied to healthy subjects.

Published
2019
Full Text
View/download PDF

40. Cardiac Magnetic Resonance Normal Reference Values of Biventricular Size and Function in Male Athlete's Heart.

Author

D'Ascenzi, Flavio, Anselmi, Francesca, Piu, Pietro, Fiorentini, Caterina, Carbone, Salvatore Francesco, Volterrani, Luca, Focardi, Marta, Bonifazi, Marco, and Mondillo, Sergio

Abstract

The aim of this meta-analysis was to derive normal reference values of biventricular size and function estimated by cardiac magnetic resonance (CMR) in competitive athletes. Exercise-induced enlargement of cardiac chambers is commonly observed in competitive athletes. However, ventricular dilatation is also a common phenotypic expression of life-threatening cardiomyopathies. The use of CMR for the exclusion of pathology is growing. However, normal reference values have not been established for athletes. The authors conducted a systematic review of English-language studies in the MEDLINE, Scopus, and Cochrane databases investigating biventricular size and function by CMR in athletes. Athletes were divided into endurance, combined, and mixed groups according to the sport practiced. The potential impact of training volume was also evaluated. Twenty-seven studies and 983 competitive athletes were included for CMR quantification of biventricular size and function. In this review, normal reference values are presented for biventricular size and function to be applied to male competitive athletes according to the disciplines practiced. A significant impact of training volume was demonstrated for the right ventricle: athletes practicing the largest number of training hours per week were those exhibiting the greatest degree of right ventricular remodeling. Notably, biventricular function was not significantly affected by training volume. The present meta-analysis defines the normal limits of biventricular size and function estimated by CMR in competitive athletes. The authors suggest using these normal reference values as an alternative to standard upper limits derived from the general population when interpreting CMR images in athletes. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

41. Next generation sequencing in sporadic retinoblastoma patients reveals somatic mosaicism

Author

Amitrano, Sara, primary, Marozza, Annabella, additional, Somma, Serena, additional, Imperatore, Valentina, additional, Hadjistilianou, Theodora, additional, De Francesco, Sonia, additional, Toti, Paolo, additional, Galimberti, Daniela, additional, Meloni, Ilaria, additional, Cetta, Francesco, additional, Piu, Pietro, additional, Di Marco, Chiara, additional, Dosa, Laura, additional, Lo Rizzo, Caterina, additional, Carignani, Giulia, additional, Mencarelli, Maria Antonietta, additional, Mari, Francesca, additional, Renieri, Alessandra, additional, and Ariani, Francesca, additional

Published
2015
Full Text
View/download PDF

42. Cerebral Circulation Time is Prolonged and Not Correlated with EDSS in Multiple Sclerosis Patients: A Study Using Digital Subtracted Angiography

Author

Monti, Lucia, primary, Donati, Donatella, additional, Menci, Elisabetta, additional, Cioni, Samuele, additional, Bellini, Matteo, additional, Grazzini, Irene, additional, Leonini, Sara, additional, Galluzzi, Paolo, additional, Severi, Sauro, additional, Burroni, Luca, additional, Casasco, Alfredo, additional, Morbidelli, Lucia, additional, Santarnecchi, Emiliano, additional, and Piu, Pietro, additional

Published
2015
Full Text
View/download PDF

43. MRI Helps Depict Clinically Undetectable Risk Factors in Advanced Stage Retinoblastomas

Author

Galluzzi, Paolo, primary, Hadjistilianou, Theodora, additional, Cerase, Alfonso, additional, Toti, Paolo, additional, Leonini, Sara, additional, Bracco, Sandra, additional, De Francesco, Sonia, additional, Galimberti, Daniela, additional, Balducci, Donatella, additional, Piu, Pietro, additional, Monti, Lucia, additional, Bellini, Matteo, additional, Caini, Mauro, additional, and Rossi, Alessandro, additional

Published
2015
Full Text
View/download PDF

48. Quantitative ColourDopplerSonography Evaluation of Cerebral Venous Outflow: A Comparative Study between Patients with Multiple Sclerosis and Controls

Author

Monti, Lucia, primary, Menci, Elisabetta, additional, Ulivelli, Monica, additional, Cerase, Alfonso, additional, Bartalini, Sabina, additional, Piu, Pietro, additional, Marotti, Nicola, additional, Leonini, Sara, additional, Galluzzi, Paolo, additional, Romano, Daniele G., additional, Casasco, Alfredo E., additional, and Venturi, Carlo, additional

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results