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2,389 results on '"Otten B"'

4. Detection of gluten peptides in human duodenal fluids with immuno-liquid chromatography-mass spectrometry.

Author

Dayon L, Núñez Galindo A, Chevalier J, Aquarius M, Otten B, Troost FJ, Duncan P, and Affolter M

Subjects
Humans, Chromatography, Liquid methods, Peptides analysis, Peptides chemistry, Glutens analysis, Glutens chemistry, Duodenum chemistry, Duodenum metabolism, Mass Spectrometry methods
Abstract

Gluten proteins are storage proteins in wheat that exhibit a certain resistance to gastrointestinal digestion. To explore solutions to cope with accidental ingestion of gluten in individuals suffering from gluten-related disorders, it is essential to monitor the fate of gluten peptides in biological samples, i.e. , gastrointestinal juices, blood plasma or urine. In this work, we aimed at developing a mass spectrometry (MS)-based method for measuring gluten peptides in human duodenal fluids. Seven gluten peptides, including the well-documented 33-mer gluten peptide (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF), were selected after a literature review and characterization of a gluten-containing product. Isotopically labelled peptides were used as references and a targeted liquid chromatography (LC) MS assay based on high resolution parallel reaction monitoring (PRM) was designed. Despite iterative and fine tuning of the LC-PRM-MS method, the low level of endogenous gluten peptides in human duodenal fluid samples precluded their direct detection. Thus, an initial immunoprecipitation (IP) step was included. Several antibodies were tested, and one proved reliable for the enrichment of the 33-mer gluten peptide as well as a few additional gluten peptides. Figures-of-merits of the immuno-LC-PRM-MS assay were assessed with a focus on quantification trueness and precision. We have developed an MS-based method for measuring the 33-mer gluten peptide in human duodenal fluids. Based on isotopic dilution, the method relies on the combination of IP and LC-PRM-MS analysis. Measurements were shown to be sensitive, quantitative, and reproducible.

Published
2024
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16. Designing a material bank to facilitate a circular construction industry

Author

Entrop, AG (Bram), Otten, B ( ), Willoughby , N ( ), and Poutiainen , S ( )

Abstract

In the project the students were asked to design a material bank to facilitate a circular construction industry. The insights they gained were used to create opportunities to renovate the road ' 'Griffiersveld” circularly. This research report was written by three students involved in the course "Industrial and sustainable building" of Saxion University of Applied Sciences.

Published
2020

17. Facultative educational exploration circular material usage Griffiersveld Apeldoorn

Author

Entrop, A.G, (Bram), Otten, B. (B.), Willoughby, N. (N.), and Poutiainen, S. (S.)

Subjects
Circular economy, Material bank, Constructionand Demolition Waste (CDW), Circularity
Abstract

This report was written for the municipality of Apeldoorn as a contribution to Cityloops. It consists of designing and planning tools and provides answers for the municipality of Apeldoorn to enable them to move towards a circular construction economy.

Published
2020

41. Intraoperative Quantification of MDS-UPDRS Tremor Measurements Using 3D Accelerometry: A Pilot Study.

Author

Smid A, Elting JWJ, van Dijk JMC, Otten B, Oterdoom DLM, Tamasi K, Heida T, van Laar T, and Drost G

Abstract

The most frequently used method for evaluating tremor in Parkinson’s disease (PD) is currently the internationally standardized Movement Disorder Society—Unified PD Rating Scale (MDS-UPDRS). However, the MDS-UPDRS is associated with limitations, such as its inherent subjectivity and reliance on experienced raters. Objective motor measurements using accelerometry may overcome the shortcomings of visually scored scales. Therefore, the current study focuses on translating the MDS-UPDRS tremor tests into an objective scoring method using 3D accelerometry. An algorithm to measure and classify tremor according to MDS-UPDRS criteria is proposed. For this study, 28 PD patients undergoing neurosurgical treatment and 26 healthy control subjects were included. Both groups underwent MDS-UPDRS tests to rate tremor severity, while accelerometric measurements were performed at the index fingers. All measurements were performed in an off-medication state. Quantitative measures were calculated from the 3D acceleration data, such as tremor amplitude and area-under-the-curve of power in the 4−6 Hz range. Agreement between MDS-UPDRS tremor scores and objective accelerometric scores was investigated. The trends were consistent with the logarithmic relationship between tremor amplitude and MDS-UPDRS score reported in previous studies. The accelerometric scores showed a substantial concordance (>69.6%) with the MDS-UPDRS ratings. However, accelerometric kinetic tremor measures poorly associated with the given MDS-UPDRS scores (R2 < 0.3), mainly due to the noise between 4 and 6 Hz found in the healthy controls. This study shows that MDS-UDPRS tremor tests can be translated to objective accelerometric measurements. However, discrepancies were found between accelerometric kinetic tremor measures and MDS-UDPRS ratings. This technology has the potential to reduce rater dependency of MDS-UPDRS measurements and allow more objective intraoperative monitoring of tremor.

Published
2022
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42. Growth hormone therapy with three dosage regimens in children with idiopathic short stature

Author

Rekers-Mombarg, L. T. M., Massa, G. G., Matranga, A. M. C., Bucker, J. M. H., Butenandt, O., Chaussain, J. L., Frisch, H., Leiberman, E., Yturriaga, R., Aarskog, D., Chatelain, P. G., Colle, M., Dacou-Voutetakis, C., Delemarre-van de Waal, H. A., Girard, F., Gosen, J. J., Irle, U., Jansen, M., Jean, R., Job, J. C., Kaar, M. L., Kollemann, F., Lenko, H. L., Muhlenberg, R., de Muinck Keizer-Schrama, S. M. P. F., Oostdijk, W., Otten, B. J., Piersson, M., Preece, M. A., Price, D. A., Rayner, P. H. W., Rouwe, C., Schmitt, K., Sippell, W. G., Blumel, P., Vulsma, T., and Waelkens, J. J. J.

Published
1998

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