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The impact of Stevia rebaudiana leaf on feeding preferences of an insect, a mite, and a mammal was investigated. The grasshopper, Valanga irregularis of the Acrididae family, avoided feeding on S. rebaudiana leaf, as evidenced by a decrease in animal weight. Increased mortality on S. rebaudiana feed was ascribed to feeding avoidance to the point of starvation. The extent of red spider mite ( Tetranychus urticae) damage was not proportional to leaf steviol glycoside (SG) concentration, a result ascribed to a feeding mechanism that avoids chlorenchyma cells that contain SGs. Guinea pigs ( Cavia porcellus) were presented with the choice between a control feed and feed amended to contain 5% sucrose or 0.02%, 4%, or 10% (dry weight) of S. rebaudiana leaf. Feed intake increased (39% above the control) for the diet involving high levels of SG amendment of feed (10% S. rebaudiana leaf). Encouragement of general mammalian herbivory may provide ecological fitness to S. rebaudiana if it is more tolerant of grazing pressure than other plants in its environment. Improvement in feed intake may have commercial implication for use of S. rebaudiana as an additive in stock feeds.

Steviol glycosides (SG) (with stevioside and rebaudioside A predominating) are present in wild-type Stevia rebaudiana, at approximately 10% of dry weight (dw), prompting a consideration of the autoecological role played by these compounds in terms of energy (C) storage and/or osmoregulation. The leaf starch pool was observed to change diurnally with respect to the light cycle (from 3.29% to 0.73% of leaf dw between dusk and dawn) and also to increase under constant light treatment (from 1.53% to 6.25% of leaf dw), while SG pools were relatively constant (around 6% w/dw). A similar trend was observed during exposure to elevated CO 2 (800 ppm), with starch increasing (from 10% to 15% of leaf dw), while SG pool size was constant (around 12% w/dw). For plants subject to increasing water stress over several days, an increase in leaf sap osmolality was observed in the leaves of a severely stressed group (from -1 MPa to -3 MPa, after 2 days of treatment), while stevioside and rebaudioside A leaf concentration was relatively constant (around 16% w/dw). These results are not consistent with a role for SG as either a short-term C store or osmoregulator in S. rebaudiana.

This study provides a comprehensive investigation on the impact of increasing NaCl concentrations on hydroponically grown Stevia rebaudiana cultivars (Shoutian-2 and Fengtian). Growth parameters including plant height, biomass and physiological responses including osmotic potential were measured. In addition, the levels of steviol glycosides, elements and primary metabolites were measured and statistically evaluated. The cultivar Fengtian grew faster, accumulated less Na + and compatible organic solutes, and more K + in the leaves, as compared to the cv. Shoutian-2. Metabolite analysis identified 81 differentially accumulated metabolites, indicating an alteration in the metabolite phenotype of both cultivars upon exposure to salinity A general increase in many amino acids, amines, sugars and sugar phosphates with a concurrent decrease in most organic acids; including tricarboxylic acid (TCA) cycle intermediates, was observed. In the more salt tolerant cv. Fengtian, the levels of hexose phosphates and metabolites involved in cellular protection increased in response to salinity. These metabolites remained unchanged in the sensitive cv. Shoutian-2. Interestingly, salt treatment notably increased the rebaudioside A concentration by 53% while at the same time stevioside decreased by 38% in Fengtian which has important implications for controlling the relative amounts of reboudioside A and stevioside. The findings of this study leads to the conclusion that mild salinity stress can increase the yield of sweetener compounds, which is dependent on the cultivar and the level of salinity stress., (Copyright © 2018 Elsevier Masson SAS. All rights reserved.)

Feeding treatments were imposed in two feeding trials involving Cobb broiler and Bond Brown layer birds. Three feed additives (biochar, bentonite and zeolite) were supplied at four rates (0, 1, 2 and 4% w/w) in feed, as previously considered in the context of animal production, was considered in the context of Excreta chemical and water retention properties and granulation characteristics of decomposed excreta (manure) were characterised. At field capacity (- 0.01 MPa), manure produced from control and 4% bentonite diets contained significantly (p = 0.001) more water (at 1.93 and 2.44% v/v water, respectively) than zeolite and biochar treatments. Manure mesoporosity was significantly (p = 0.015) higher in 2 and 4% bentonite treatments than other feed additives. Fresh excreta from layer birds on the control diet contained 6% w/dw N and 35% C, which was decreased to 2.6% N and 28% C after decomposition, with C:N ratio changing from 5.9 to 12.1. Ammonia loss was higher from biochar and zeolite manures than control or bentonite, associated with higher pH in the biochar and zeolite manures. More N was unaccounted from bentonite manure than other treatments, presumably lost as N 2 O or N 2 , a result linked to its higher moisture content and its enhanced rate of denitrification. The highest proportion of granules in the size class desired for fertilizer spreading was achieved using decomposed manure from the 1 and 2% w/w biochar treatments of the broiler trial, and 1 and 2% zeolite and 4% biochar treatments of the layer trial. Thus the feed amendments improved poultry manure in specific ways., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

According to the World Health Organisation, antibiotics are rapidly losing potency in every country of the world. Poultry are currently perceived as a major source of pathogens and antimicrobial resistance. There is an urgent need for new and natural ways to control pathogens in poultry and humans alike. Porous, cation rich, aluminosilicate minerals, zeolites can be used as a feed additive in poultry rations, demonstrating multiple productivity benefits. Next generation sequencing of the 16S rRNA marker gene was used to phylogenetically characterize the fecal microbiota and thus investigate the ability and dose dependency of zeolite in terms of anti-pathogenic effects. A natural zeolite was used as a feed additive in laying hens at 1, 2, and 4% w/w for a 23 week period. At the end of this period cloacal swabs were collected to sample faecal microbial communities. A significant reduction in carriage of bacteria within the phylum Proteobacteria, especially in members of the pathogen-rich family Enterobacteriaceae, was noted across all three concentrations of zeolite. Zeolite supplementation of feed resulted in a reduction in the carriage of a number of poultry pathogens without disturbing beneficial bacteria. This effect was, in some phylotypes, correlated with the zeolite concentration. This result is relevant to zeolite feeding in other animal production systems, and for human pathogenesis., (Copyright © 2016 Elsevier GmbH. All rights reserved.)

A range of feed supplements, including antibiotics, have been commonly used in poultry production to improve health and productivity. Alternative methods are needed to suppress pathogen loads and maintain productivity. As an alternative to antibiotics use, we investigated the ability of biochar, bentonite and zeolite as separate 4% feed additives, to selectively remove pathogens without reducing microbial richness and diversity in the gut. Neither biochar, bentonite nor zeolite made any significant alterations to the overall richness and diversity of intestinal bacterial community. However, reduction of some bacterial species, including some potential pathogens was detected. The microbiota of bentonite fed animals were lacking all members of the order Campylobacterales. Specifically, the following operational taxonomic units (OTUs) were absent: an OTU 100% identical to Campylobacter jejuni; an OTU 99% identical to Helicobacter pullorum; multiple Gallibacterium anatis (>97%) related OTUs; Bacteroides dorei (99%) and Clostridium aldenense (95%) related OTUs. Biochar and zeolite treatments had similar but milder effects compared to bentonite. Zeolite amended feed was also associated with significant reduction in the phylum Proteobacteria. All three additives showed potential for the control of major poultry zoonotic pathogens.

Silverleaf whitefly (SLW, Bemisia tabaci MEAM1) and aphids are sap-sucking insects, which pose a serious threat to Australian cucurbit crops and the horticulture industry. Traditional chemical control for these insect pests is becoming less effective, and there is a need to search for alternative or supplementary methods. This study aimed to manipulate the habitat of pumpkin crops in a tropical setting (Queensland, Australia), by growing pumpkins (var. Japanese pumpkin) alone and between lablab (Lablab purpureus L. Sweet). It was hypothesized that the presence of lablab will increase the populations of natural enemies, and through their control of insect pests such as SLW and aphids, will affect pumpkin yield. The population of arthropods (natural enemies and pests of pumpkin), with a focus on SLW and aphids, were sampled weekly on both lablab and pumpkin crop for a total of 21 weeks. Results showed that lablab hosted more enemies of SLW per plant than pumpkin in either treatment. In addition, adult SLW numbers were significantly higher in the pumpkin-only crop compared with the pumpkin grown between lablab, while pumpkin in the mixed plantings had significantly more ladybirds and lacewing larvae (P < 0.05). While there was no significant difference in the average fruit weight between treatments, the total weight (kg) and number of marketable pumpkins per hectare was greater (P < 0.05) for the pumpkin/lablab treatment than the pumpkin-only treatment. This study shows that growing lablab alongside a pumpkin crop may enhance natural enemies of SLW and could significantly increase the yield.

Nickel (Ni), an essential heavy metal, is transformed from an element with vital functions to a hazardous contaminant within agricultural environments, owing to its extensive utilization across diverse applications, spanning from everyday household items to various industrial processes. Understanding Ni’s bioavailability in soil, associated risks, phytodetoxification processes, and current remediation techniques is crucial for sustainable ecosystem management. Although Ni occurs naturally in very small concentrations in soil, water, and air, its absorption by plants from soil to roots is vital for both activating enzymes and supporting plant growth. Ni plays pivotal roles in numerous physiological functions, including photosynthesis, nitrogen metabolism, and growth regulation. However, excessive Ni can induce toxic effects in plants through the production of reactive oxygen species (ROS) that interferes with their biochemical, physiological, and morphological processes. Eventually, there are only a number of feasible strategies for the mitigation of Ni-polluted soils. The present review critically examines the origins and dynamics of Ni in soil–plant systems and its detrimental effects on plants at morphological, biochemical, physiological, and molecular levels. The review also explores the detoxification mechanisms in higher plants and current remediation technologies for decision-making and sustainable management of Ni contamination in the environment. The review concludes that there is a need of friendly effective and environmentally sustainable technologies for the mitigation of Ni toxicity in terrestrial ecosystems and environmental sustainability. [ABSTRACT FROM AUTHOR]

This overview focuses on the use of 15N labeled fertilizers to provide information about nitrogen (N) cycling processes in conventional vegetable production systems. Recent interest in determining fertilizer N use efficiency (FNUE) for vegetables may be due to the increase of global vegetable production, rise in N fertilizer costs, consumer preference for diversified plant-based diets, and interest in purchasing sustainably produced fresh foods. Vegetable production is known to require intensive amounts of N fertilizers, where the inefficiencies in N utilization can reach lower than 50%. To understand such systems comprehensively, the 15N technique was found helpful to investigate the co-factors involved in plant N uptake and identify sustainable solutions for economic vegetable production. Through a summary of the 15N studies, FNUE was found to range between 9 and 67%, with an average of 23% to 34%. Therefore, in most cases, over 70% of the applied N remained as residual or unaccounted for N in the soil post-harvest, which poses a significant risk for nitrate leaching and nitrous oxide emissions in vegetable systems. Despite advancements in production technologies, such as drip-irrigated plasticulture systems, inefficiencies persist due to excessive N fertilizer application and suboptimal timing of applications. While the cost of 15N-labeled fertilizers can be high, their use has provided critical insights into realistic N management scenarios, thus enhancing our understanding of important N transformation processes in vegetable systems. Additionally, the 15N natural abundance (δ15N) method facilitated the qualitative study of complex systems, where accurately labeling material such as enhanced efficiency fertilizers (EEFs), can be challenging with traditional 15N enrichment techniques. Further innovation of the 15N technique is necessary to study sustainable N management practices, including the use of EEFs, biochar, plant growth-promoting bacteria, and nitrification inhibitors, which are of increasing interest to the industry. Key takeaways from the 15N fertilizer studies emphasize the importance of improving N management strategies that optimize the source, placement, timing, and rate of N application to improve FNUE, minimize N pollution, and reduce reliance on economically prohibitive remediation approaches. This overview highlights the need for proactive and preventive N management strategies that reduce environmental impacts while enhancing the efficiency of vegetable production systems. [ABSTRACT FROM AUTHOR]

The rapid growth of Bamboo made the uptake and allocation of nitrogen much important. Nitrate is the main form that plant utilized nitrogen by nitrate transporters (NRTs) as well as ammonium salt. In this study, we identified 155 DfNRT genes which mapped to 32 chromosomes out of 35 chromosomes in Dendrocalamus farinosus. Collinearity analysis showed most NRT genes in D. farinosus paired with NRT genes in D. farinosus and P. edulis, which another two sequenced woody bamboo species, and the divergence was similar to the woody bamboo whole-genome duplication event. Through the 15N-nitrate trace analysis, we found that the nitrogen absorbed by roots in D. farinosus was preferentially distributed to above-ground parts, especially transported to leaves. DfNPF2.13 and DfNPF6.9 exhibited higher expression in leaf, and upregulated with extra N supply, suggesting they might be participating in N allocation between leaves in D. farinosus. This study provides a foundation for understanding the mechanism of nitrate transport and distribution in bamboo, and provide valuable information for improving bamboo nitrate absorption and promoting efficient nitrogen utilization. [ABSTRACT FROM AUTHOR]

In the present scenario, waste management, especially agro-waste, is one of the major challenges. India is an agrarian country and the economy depends on agriculture and a huge amount of agro-wastes are generated. In this study, biochar was prepared from paddy straw and sugarcane bagasse and was used in the feed of genetically improved farm tilapia (GIFT) at 0.5% (w/w) basis to study its effect on growth and haematological parameters. An experiment was carried out in triplicate in 500-l capacity FRP tanks comprising two treatments and one control. Tanks were filled with inland saline soil to maintain 25-cm soil's bed and water with 12 ppt salinity. A total of 22 fish having an average length of 5.14 ± 0.07 cm and weight of 4.8 ± 0.05 g were stocked in each tank; the fish were fed at apparent satiation level, twice daily for 45 days. It was observed that growth parameters like SGR% (specific growth rate), weight gain (WG%), and feed conversion ratio (FCR) were significantly improved (p < 0.05) than the control. Biochar-enriched feed also significantly improved the haematological parameters like red blood cell (RBC), white blood cell (WBC), haemoglobin (Hb) haematocrit % (HCT), mean cell haemoglobin concentration (MCH), and MCV (mean cell volume) in treatment groups than the control. Feeding biochar as feed additives was also found to decrease catalase (CAT) and enhanced amylase and lipase activities in treatments as compared to control (p < 0.05). It can be concluded that the application of biochar as a feed additive enhanced the growth and overall health of the fish, and it can enhance fish production. However, biochar from sugarcane bagasse was found to be more effective than the paddy straw biochar in the diet of tilapia (GIFT). [ABSTRACT FROM AUTHOR]

Microbially enhanced compost extracts ('compost tea') are being used in commercial agriculture as a source of nutrients and for their perceived benefit to soil microbiology, including plant disease suppression. Rumen content material is a waste of cattle abattoirs, which can be value-added by conversion to compost and 'compost tea'. A system for compost extraction and microbial enhancement was characterised. Molasses amendment increased bacterial count 10-fold, while amendment based on molasses and 'fish and kelp hydrolysate' increased fungal count 10-fold. Compost extract incubated at 1:10 (w/v) dilution showed the highest microbial load, activity and humic/fulvic acid content compared to other dilutions. Aeration increased the extraction efficiency of soluble metabolites, and microbial growth rate, as did extraction of compost without the use of a constraining bag. A protocol of 1:10 dilution and aerated incubation with kelp and molasses amendments is recommended to optimise microbial load and fungal-to-bacterial ratio for this inoculum source., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

A comparative study was performed on compost extracts prepared from cattle rumen content composted for three and nine months, nine month old compost inoculated with a Nutri-Life 4/20™ inoculum, and two commercial preparations (LivingSoil™ and Nutri-Life 4/20™), all incubated for 48h. Nutri-Life 4/20™ had the highest concentrations of NO(3)(-)-N and K(+)-K, while rumen compost extract had higher humic and fulvic acids concentration. The bacterial and fungal community level functional diversity of three month old compost extract and of LivingSoil™, assessed with Biolog™, were higher than that of nine month old rumen compost extract, with or without Nutri-Life 4/20™ inoculum, or Nutri-Life 4/20™. No difference in fungal diversity was observed between treatments, as indicated by Denaturing Gradient Gel Electrophoresis (DGGE) analysis, however, bacterial diversity was higher in all compost extracts and LivingSoil™ compared to the Nutri-Life 4/20™. Criteria for judging the quality of a microbially enhanced extract are discussed., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Physico-chemical and microbiological investigations were carried out on rumen content material composted for nine months, fresh vermicasts (obtained after passing the same compost through the guts of a mixture of three species of earthworms: Eisenia fetida, Lumbricus rubellus and Perionyx excavates) and microbially enhanced extracts derived from rumen compost, vermicast and vermicast leachate incubated for up to 48 h. Compared to composted rumen contents, vermicast was only improved in terms of microbial biomass C, while vermicast leached extract was significantly higher in NH(4)(+)-N,PO(4)(-)-P, humic acid, bacterial counts and total microbial activity compared to rumen compost extract. Although no difference between treatments was observed in genetic diversity as indicated by DGGE analysis, community level functional diversity of vermicast leached extract (Biolog™) was higher than that of composted rumen contents, vermicast and rumen compost extract indicating an enhancement of microbial activity rather than diversity due to liquid incubation., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

Cucurbit crops host a range of serious sap-sucking insect pests, including silverleaf whitefly (SLW) and aphids, which potentially represent considerable risk to the Australian horticulture industry. These pests are extremely polyphagous with a wide host range. Chemical control is made difficult due to resistance and pollution, and other side-effects are associated with insecticide use. Consequently, there is much interest in maximising the role of biological control in the management of these sap-sucking insect pests. This study aimed to evaluate companion cropping alongside cucurbit crops in a tropical setting as a means to increase the populations of beneficial insects and spiders so as to control the major sap-sucking insect pests. The population of beneficial and harmful insects, with a focus on SLW and aphids, and other invertebrates were sampled weekly on four different crops which could be used for habitat manipulation: Goodbug Mix (GBM; a proprietary seed mixture including self-sowing annual and perennial herbaceous flower species); lablab (Lablab purpureus L. Sweet); lucerne (Medicago sativa L.); and niger (Guizotia abyssinica (L.f.) Cass.). Lablab hosted the highest numbers of beneficial insects (larvae and adults of lacewing (Mallada signata (Schneider)), ladybird beetles (Coccinella transversalis Fabricius) and spiders) while GBM hosted the highest numbers of European bees (Apis mellifera Linnaeus) and spiders. Lucerne and niger showed little promise in hosting beneficial insects, but lucerne hosted significantly more spiders (double the numbers) than niger. Lucerne hosted sig-nificantly more of the harmful insect species of aphids (Aphis gossypii (Glover)) and Myzus persicae (Sulzer)) and heliothis (Heliothis armigera Hübner). Niger hosted significantly more vegetable weevils (Listroderes difficillis (Germar)) than the other three species. Therefore, lablab and GBM appear to be viable options to grow within cucurbits or as field boundary crops to attract and increase beneficial insects and spiders for the control of sap-sucking insect pests. Use of these bio-control strategies affords the opportunity to minimise pesticide usage and the risks associated with pollution.

Impacts of salinity become severe when the soil is deficient in oxygen. Oxygation (using aerated water for subsurface drip irrigation of crop) could minimize the impact of salinity on plants under oxygen-limiting soil environments. Pot experiments were conducted to evaluate the effects of oxygation (12% air volume/volume of water) on vegetable soybean (moderately salt tolerant) and cotton (salt tolerant) in a salinized vertisol at 2, 8, 14, 20 dS/m EC(e). In vegetable soybean, oxygation increased above ground biomass yield and water use efficiency (WUE) by 13% and 22%, respectively, compared with the control. Higher yield with oxygation was accompanied by greater plant height and stem diameter and reduced specific leaf area and leaf Na+ and Cl- concentrations. In cotton, oxygation increased lint yield and WUE by 18% and 16%, respectively, compared with the control, and was accompanied by greater canopy light interception, plant height and stem diameter. Oxygation also led to a greater rate of photosynthesis, higher relative water content in the leaf, reduced crop water stress index and lower leaf water potential. It did not, however, affect leaf Na+ or Cl- concentration. Oxygation invariably increased, whereas salinity reduced the K+ : Na+ ratio in the leaves of both species. Oxygation improved yield and WUE performance of salt tolerant and moderately tolerant crops under saline soil environments, and this may have a significant impact for irrigated agriculture where saline soils pose constraints to crop production.

Within-plant light and nutrient environments are spatially and temporally heterogeneous. The development of different parts of a plant is highly coordinated, which enables the efficient capture and use of resources in such heterogeneous environments. The physiological mechanisms underlying the correlative control of distantly located plant tissues and organs are still not fully understood. In this study, a mathematical model based on a self-organisation mechanism for resource allocation mediated by polar auxin transport is proposed to explain the origin of correlative effects among shoot branches. In the model, the shoot system of an individual plant is treated as a collection of relatively independent modular subunits competing for root-derived resources. The allocation of root-derived resources to different parts of the shoot is determined by their relative vascular contacts with the root system. The development of the vascular network is specified by the polar transport of auxin produced by various parts of the shoot in response to their immediate internal and external environments. The simulation results show that, by altering the amount of auxin they release individually in response to the local environment and modifying their relative vascular contact with the root system, subunits of a shoot are able to coordinate without a central controller and self-organise into functional and structural patterns such as light foraging and correlative dominance. This modelling study suggests that morphological dynamics at the whole-plant level can be understood as the sum of all modular responses to their local environments. The concept of self-organisation holds great promise for an in-depth understanding of the organisational laws that generate overall plant structure and functions.

The stability-related asymmetry in roots, trunk, and crown is always found as a typical effect of biomechanical design under heterogeneous stimulus environment. However, it appears to be a conflict between the biomechanical principle and the source-sink distance of nutrient allocation strategies when the orientational asymmetry occurs. Adaptive growth strategies associated with biomass and nutrient allocation remain to be explored. This study used both the minirhizotron and harvest methods to test the effect of trunk inclination of camphor trees (Cinnamomum camphora) and found that the asymmetry coefficient of root biomass was − 0.29, showing more root biomass distributed on the other side of trunk inclination. This side had larger surface area and volume of fine roots, the smaller in diameter and the larger in length of the first level roots, higher leaf total nitrogen (TN) and slightly higher root TN content, higher activities of antioxidant enzymes SOD, POD, and CAT in leaves, and lower soluble sugar and protein. The biomass, morphological and physiological characteristics suggest that trees may follow both the biomechanical design and source-sink distance of nutrient allocation strategies. The research results expand the connotation of root-shoot balance in the orientational allocation of biomass and physiological responses. [ABSTRACT FROM AUTHOR]

In recent decades an extensive mortality and decline of Quercus suber populations mainly caused by Phytophthora cinnamomi has been observed. In the current study, a chestnut gene homologous to ginkbilobin-2 (Cast_Gnk2-like), which in Ginkgo biloba codifies an antifungal protein, was transferred into cork oak somatic embryos of three different embryogenic lines by Agrobacterium mediated transformation. The transformation efficiency varied on the genotype from 2.5 to 9.2%, and a total of 22 independent transformed lines were obtained. The presence of Cast_Gnk2-like gene in transgenic embryos was verified in all lines by PCR. The number of transgene copies was estimated by qPCR in embryogenic lines with high proliferation ability and it varied between 1 and 5. In addition, the expression levels of Cast_Gnk2-like gene were determined in the embryogenic lines, with higher levels in lines derived from the genotype ALM6-WT. Transgenic plants were obtained from all transgenic lines and evaluated after cold storage of the somatic embryos for 2 months and subsequent transfer to germination medium. In vitro tolerance tests made under controlled conditions and following zoospore treatment showed that plants overexpressing Cast_Gnk2-like gene improved tolerance against Pc when compared to wild type ones. [ABSTRACT FROM AUTHOR]

Eleocharis dulcis (Burm. f.) Trin. ex Hensch., commonly known as Chinese water chestnut, is a traditional aquatic vegetable in China, and now is widely cultivated throughout the world because of its high nutritional value and unique tastes. Here, we report the assembly of a 493.24 Mb telomere-to-telomere (T2T) genome of E. dulcis accomplished by integrating ONT ultra-long reads, PacBio long reads and Hi-C data. The reference genome was anchored onto 111 gap-free chromosomes, containing 48.31% repeat elements and 33,493 predicted protein-coding genes. Whole genome duplication (WGD) and inter-genomic synteny analyses indicated that chromosome breakage and genome duplication in E. dulcis possibly occurred multiple times during genome evolution after its divergence from a common ancestor with Rhynchospora breviuscula at ca. 35.6 Mya. A comparative time-course transcriptome analysis of corm development revealed different patterns of gene expression between cultivated and wild accessions with the highest number of differentially expressed genes (DEGs, 15,870) at the middle swelling stage and some of the DEGs were significantly enriched for starch metabolic process. [ABSTRACT FROM AUTHOR]

The hypothesis that hyperaccumulation of certain metals in plants may play a role in osmotic adjustment under water stress (drought) was tested in the context of nickel hyperaccumulator Stackhousia tryonii. Field-collected mature plants of S. tryonii, grown in native ultramafic soil, were pruned to soil level and the re-growth exposed to five levels of water stress (20, 40, 60, 80 and 100% field capacity; FC) for 20 weeks. Water stress had significant (P<0.05) influence on growth (biomass), water potential and shoot Ni concentrations, with progressively more impact as water stress was increased from 80 to 40% FC. Shoot Ni concentration increased significantly from 3,400 microg g(-1) dry weight (at 100% FC) to 9,400 microg g(-1) dry weight (at 20% FC). Assuming that Ni is uniformly distributed through the shoot tissue, the Ni concentration could account for 100% at the 80 and 60% FC conditions, and 50% at the 40 and 20% FC conditions of plant osmotic regulation. The results are consistent with a role of Ni in osmotic adjustment and protection of S. tryonii plants against drought.

Background and Aims: Nitrate is the major nitrogen source for many plants. The first step of the nitrate assimilation pathway is the reduction of nitrate to nitrite, catalysed by nitrate reductase (NR). Circadian oscillations in expression and activity of NR have been demonstrated in many plant species. The pathway by which this circadian behaviour is regulated remains to be elucidated. In this study, based on recent experimental observations, a mathematical model is proposed to explain the origin of diurnal and circadian oscillations in NR gene expression and enzyme activity., Methods: The dynamic model is based on the feedback interconnections between NR and its substrate, nitrate. In the model, NR activity is regulated at the transcriptional level, in response to the balance between nitrate influx and reduction, and at the post-translational levels in response to signals from carbon assimilation. Conditions for the model system to generate self-sustained circadian oscillations are investigated by numerical simulations., Key Results and Conclusions: Under light/dark cycles, the simulation results are in agreement with the observed diurnal pattern of changes in leaf nitrate concentration, NR transcript level and NR activity. Within a range of kinetic parameter values, circadian oscillation behaviour persists even under constant light, with periods of approx. 24 h. These simulation results suggest that sustained circadian oscillations can originate from the feedback interactions between NR and its substrate, nitrate, without the need to postulate the existence of an endogenous 'circadian clock'.

Background and Aims: Stackhousia tryonii, a rare nickel hyperaccumulating herb, is endemic to ultramafic (serpentine) soils of central Queensland, Australia. The effects of eight dormancy-relieving treatments on germination of stored seeds of Stackhousia tryonii were investigated under controlled light and temperature conditions. *, Methods: The treatments were: untreated (control i), leached and dehydrated (primed control ii), treating with gibberellic acid (150 and 300 microM), smoke extract (5 and 10 %, v/v) and potassium cyanide (40 and 80 mM). *, Key Results: Freshly harvested seeds did not germinate. Germination percentage increased with time of storage for up to 18 months (38.3 %). Gibberellin, smoke extract and cyanide treatments did not significantly improve germination. Light did not affect seed germination and there was no interaction between dormancy-relieving treatments and light. A significant inhibition of germination occurred in seeds treated with 5 % (but not 10 %) aqueous smoke extract. Saturated fatty acids, predominantly tridecanoic (C13:0), constituted about 90 % of the total fatty acids in the oil of freshly harvested seeds. In contrast, there was increased accumulation (>75 %) of mono-unsaturated (oleic, c18:1) and poly-unsaturated (linoleic, c18:2; linolenic, c18:3) fatty acids in the oil of stored seeds. *, Conclusions: Seeds of S. tryonii require an after-ripening period for germination.

The Central Queensland University (CQU) has championed a self-contained concrete lined evapotranspiration channel. Any non-transpired effluent returns to a holding tank and is recirculated through the evapotranspiration channel until it is used. This paper examines the results from the Rockhampton trial site. Nutrient ions in the effluent were quantified over time and found not to accumulate in solution. Microbial analysis of the treated effluent was performed and was found to be within the ranges required by the relevant legislative codes. Citrus fruit grown in the evapotranspiration channel were sampled and no elevated levels of faecal coliforms were recorded. Macronutrients and micronutrients of the soil in the channels were measured over a 5-year period. No toxic accumulations or nutrient deficiencies in the soil occurred. Levels of salinity and sodicity in the evapotranspiration channel soil were quantified. Salinity rose slightly, as did sodium. Concentrations of salts and sodium did not reach unsustainable levels. The aim of the trial was to develop an on-site treatment and reuse system that is sustainable and protects public and environmental health.

Main Conclusion: The combination of water and gas at an aeration rate of 15 mg/L and irrigation amount of 0.8 Ep significantly promoted the root morphology, inter-root soil bacterial community structure and diversity of pepper, enhanced the structure of molecular symbiotic network, and stimulated the potential ecosystem function. Poor aeration adversely affects the root morphology of pepper (Capsicum annuum L.) and bacterial community. It is critical to understand the effects of water-air interactions on root morphology and bacterial community structure and diversity. A randomized block experiment was conducted under the two aeration rates of dissolved oxygen mass concentrations, including A: 15 mg/L, O: 40 mg/L, and C: non-aeration as control treatment, and two irrigation rates of W 1 and W 2 (0.8 Ep and 1.0 Ep). The results showed that aerated irrigation had a significant effect on the root morphology of pepper. Compared with treatment CW 1 , treatment AW 1 increased root dry weight, root length, root volume, and root surface area by 13.63%, 11.09%, 59.47%, and 61.67%, respectively (P < 0.05). Aerated irrigation significantly increased the relative abundance of Actinobacteria, Gemmatimonadetes, Alphaproteobacteria, Gemmatimonas, Sphingomonas, and KD4-96 aerobic beneficial bacteria. It decreased the relative abundance of Proteobacteria, Monomycetes, Bacteroidetes, Corynebacterium, Gammaproteobacteria, Anaerolineae, Subgroup&#95;6, MND1, Haliangium, and Thiobacillus. The Pielou&#95;e, Shannon and Simpson indexes of treatment AW 1 were significantly higher than treatments OW 1 and CW 1 . The results of the β-diversity of bacterial communities showed that the structure of soil bacterial communities differed significantly among treatments. Actinobacteria was a key phylum affecting root morphology, and AW 1 treatment was highly correlated with Actinobacteria. Molecular ecological network analysis showed a relatively high number of bacterial network nodes and more complex relationships among species under the aeration of level 15 mg/L and 0.8 Ep, as well as the emergence of new phylum-level beneficial species: Dependentiae, BRC1, Cyanobacteria, Deinococcus-Thermus, Firmicutes, and Planctomycetes. Therefore, the aeration of 15 mg/L and 0.8 times crop-evaporation coefficient can increase root morphology, inter-root soil bacterial community diversity and bacterial network structure, and enhance potential ecosystem functions in the rhizosphere., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Aims: The objective of this study was to propose a maize–peanut intercropping system for traditional sole maize to increase economic benefits and decrease environmental pollution for dryland farming. Methods: The treatments comprised sole maize, sole peanut, maize–peanut intercropping, maize–peanut intercropping–rotation (rotation of the maize and peanut planting strips every year), and maize–peanut intercropping–rotation with 20% and 40% N reduction in maize planting strip. Soil water and total N stock, photosynthetic traits, yield, greenhouse gas emissions, economic income, and interspecific relationships were evaluated. Results: Intercropped maize absorbed more moisture but lost more N than sole maize. Maize–peanut intercropping had higher economic benefits and less N2O emissions than sole maize, but had no intercropped advantage on land equivalent ratio. In addition, intercropping increased the continuous cropping barriers of peanuts than a sole peanut. Rotation of the planting strips in intercropping increased the peanut yield and economic benefits compared with not rotating the planting strips, and the economic benefits reached the highest level in the third planting year. A 20% N reduction in maize planting strips can decrease the global warming potential without affecting economic benefits. A 40% N reduction in maize planting strips could reduce soil N stock, maize yield, water use efficiency, N use efficiency, land equivalent ratio, and income. Conclusions: A maize–peanut intercropping–rotation system is recommended for dryland farming since it promoted efficient use of N and economic benefits and decreased N2O emission. The proper N reduction in maize planting does not lead to N stress but decreased global warming potential. [ABSTRACT FROM AUTHOR]

The micro and nano bubble (MNB) technology, due to its promising features and advantages, has become increasingly popular in agriculture. MNB-treated water positively impacts plant growth, especially when it is treated with a combination of gas-like carbon dioxide (CO 2 ), injected through the MNB generator. Therefore, this study used MNB water with CO 2 that are small bubbles of nanometer and micrometer diameters having several unique physical properties that make them useful for water treatments. This research evaluates the effect of MNBs and CO 2 -treated water on leafy vegetable Amaranth green (Amaranthus viridis). The experiment divided the Amaranth plants into three major groups, G1, G2, and G3, irrigated by MNB water with dissolved CO 2 , MNBs with only Air, and simple tap water, respectively. The first treatment group (G1) (MNBs with CO 2 ) was further divided into three sub-divisions, i.e., G1A, G1B, G1C, and the second treatment group G2 (MNBs with Air) was divided into three sub-groups, i.e., G2A, G2B, and G2C, while the third group G3 with only one category as only controlled group. These sub-divisions of treatment groups G1 and G2 were done to investigate the impact of MNBs and CO 2  treated water with different time durations. For example, in G1A, the water treatment with MNBs and CO 2 was kept five minutes, for G1B 10 minutes, and G1C 15 minutes. Similar method was adopted for G2 as well. According to the results, water treated with MNB and CO 2 has a significant (90%) impact on the Amaranth germination rate and plant growth. Specifically, pots irrigated with the MNBs + CO 2 -treated water showed better germination and plant growth rate than the MNBs + Air treated water. Overall, both treatment groups, G1 and G2, showed significantly higher impacts than the CK groups (simple water). Further, this experiment showed that the 10 and 15 minutes treatment of water (G1B, G1C and G2B, G2C) increased the stem height and root size compared to the 5 minutes treated water (G1A, G2A). This study concludes that the water with MNBs has a positive impact on the vegetables and can be an effective technology to improve crop yield., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Key Message: Candidate male sterility genes were identified in sugarcane, which interacts with kinase-related proteins, transcription factors, and plant hormone signaling pathways to regulate stamen and anther development. Saccharum officinarum is a cultivated sugarcane species that its predominant feature is high sucrose content in stems. Flowering is necessary for breeding new cultivars but will terminate plant growth and reduce sugar yield. The wild sugarcane species Saccharum spontaneum has robust and viable pollen, whereas most S. officinarum accessions are male sterile, which is a desirable trait of a maternal parent in sugarcane breeding. To study male sterility and related regulatory pathways in sugarcane, we carried out RNAseq using flowers in different developmental stages between male-sterile S. officinarum accession 'LA Purple' and fertile S. spontaneum accession 'SES208'. Gene expression profiles were used to detect how genes are differentially expressed between male sterile and fertile flowers and to identify candidate genes for male sterility. Weighted gene correlation networks analysis (WGCNA) was conducted to investigate the regulatory networks. Transcriptomic analyses showed that 988 genes and 2888 alleles were differentially expressed in S. officinarum compared to S. spontaneum. Ten differentially expressed genes and thirty alleles were identified as candidate genes and alleles for male sterility in sugarcane. The gene Sspon.03G0007630 and two alleles of the gene Sspon.08G0002270, Sspon.08G0002270-2B and Sspon.08G0014700-1A, were involved in the early stamen or carpel development stages, while the remaining genes were classified into the post-meiosis stage. Gibberellin, auxin, and jasmonic acid signaling pathways are involved in the stamen development in sugarcane. The results expanded our knowledge of male sterility-related genes in sugarcane and generated genomic resources to facilitate the selection of ideal maternal parents to improve breeding efficiency., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Hailstorms pose a direct threat to agriculture, often causing yield losses and worsening farmers' agricultural activity. Traditional methods of hail damage estimation, conducted by insurance field inspectors, have been questioned due to their complexity, partial subjectivity, and lack of accounting for spatial variability. Therefore, remote sensing integration in the estimation process could provide a valuable aid. The focus of this study was on winter wheat (Triticum aestivum L.) and its response to damage in the near-infrared (NIR) spectral region, with a particular emphasis on the study of brown pigments as a proxy for yield damage estimation and mapping. An experiment was conducted during two cropping seasons (2020–2021 and 2021–2022) at two sites, simulating hail damage at critical flowering and milky stages using a specifically designed prototype machinery with low, medium, and high damage gradients compared to undamaged conditions in plots with a minimum of 400 m2 area. After the damage simulation, hyperspectral visible-NIR reflectance was measured with Unmanned Aerial Vehicle (UAV) flights, and measurements of chlorophyll and of leaf area index (LAI) were contextually taken. Final yield per treatment was recorded using a combine. An increase in absorbance in the NIR region (780–950 nm) was observed and evaluated using a spectral mixture analysis (SMA) after selecting representative damaged and undamaged vegetation spectra to map the damage. The abundance of damaged endmember pixels per treatment resulted in a good relationship with the final yield (R2 = 0.73), identifying the most damaged areas. The absorbance feature was further analysed with a newly designed multispectral index (TAI), which was tested against a selection of indices and resulted in the highest relationship with the final yield (R2 = 0.64). Both approaches were effective in highlighting the absorbance feature over different dates and development stages, defining an effective mean for hailstorm damage mapping in winter wheat. [ABSTRACT FROM AUTHOR]

Agricultural diversification and high-quality products deriving from sustainable crops such as hemp can represent a solution to revitalize marginal areas and reverse land abandonment. This study aimed at comparing four different hemp cultivars (Carmagnola Selezionata, "CS"; Futura 75, "FUT"; Felina 32, "FEL"; Secuieni Jubileu, "JUB") to provide information to select the best suited cultivar for cultivation in mountain marginal areas and for specific end-use applications. Hemp cultivars were cultivated in a single experimental field to compare their ecological and agronomic behavior (duration of life cycle phases, plant size and biomass allocation, and plant resource-use strategies). Through metabolomic analysis of both vegetative and reproductive parts of the plants we tested the presence of substances of nutraceutical interest and traced seed nutritional profile. The four cultivars had different ecological and agronomic behavior, and nutritional profile. We found several compounds with potential pharmaceutical and nutraceutical values in all parts of the plant (leaves, inflorescences, and stems). JUB resulted the most suitable for seed production while CS showed the highest content of bioactive compounds in flowers and leaves. FUT, showed the best suitability for multi-purpose cultivation, while FEL seemed to be not appropriate for the cultivation in mountain area. The multi-disciplinary approach we adopted was effective in distinguish across hemp cultivars and provided information to farmers for the selection of the best hemp cultivar to select. Hemp had a high potential for cultivation in marginal lands, demonstrating to be an economic resource due to its multi-purpose use and to the possibility to generate high-added values products. Our results could serve as a stimulus for the reintroduction of this culture in the study area and in other similar environments. [ABSTRACT FROM AUTHOR]

Considering fast-changing environment, emerging pathogens, and the imminent need to feed a global population that is predicted to increase to 9–10 billion people by the year 2050, plant breeders are faced with the challenge of exploring more efficient crop improvement strategies. The urgency to enhance crops under these conditions has become a paramount concern for scientists worldwide, as current crop enhancement projects progress at a pace insufficient to meet the growing food demand. Traditional breeding methods, which often take over 10 years to develop high-performing cultivars with desired traits, are proving to be inadequate. However, a new approach known as Speed breeding presents a game-changing opportunity for crop improvement in the face of a changing world offering the potential to significantly accelerate the development, marketing, and commercialization of improved plant varieties. Speed breeding, a methodology that manipulates temperature, light duration, and intensity to accelerate plant development, has emerged as a promising solution for achieving climate resilience, long-term yield, and nutritional security. Recent innovations in breeding technologies, including genotyping, marker-assisted selection (MAS), high throughput phenotyping, genomic selection (GS), overexpression/knock-down transgenic techniques, and genome editing, can be combined with speed breeding to achieve more precise and expedited outcomes in crop enhancement. This review explores the key opportunities and challenges associated with speed breeding to guide pre-breeding and breeding programs. To achieve more efficient outcomes in enhancing major food crops, this review highlights various alternative approaches and strategies adopted for speed breeding. Integrating speed breeding with existing technologies will be essential for future crop breeding success, and concerted efforts and ongoing research holds the potential to pave the way for a resilient and productive agricultural future. [ABSTRACT FROM AUTHOR]

Root and tuber crop breeding is at the front and center of CIP’s science program, which seeks to develop and disseminate sustainable agri-food technologies, information and practices to serve objectives including poverty alleviation, income generation, food security and the sustainable use of natural resources. CIP was established in 1971 in Peru, which is part of potato’s center of origin and diversity, with an initial mandate on potato and expanding to include sweetpotato in 1986. Potato and sweetpotato are among the top 10 most consumed food staples globally and provide some of the most affordable sources of energy and vital nutrients. Sweetpotato plays a key role in securing food for many households in Africa and South Asia, while potato is important worldwide. Both crops grow in a range of conditions with relatively few inputs and simple agronomic techniques. Potato is adapted to the cooler environments, while sweetpotato grows well in hot climates, and hence, the two crops complement each other. Germplasm enhancement (pre-breeding), the development of new varieties and building capacity for breeding and variety testing in changing climates with emphasis on adaptation, resistance, nutritional quality and resource-use efficiency are CIP’s central activities with significant benefits to the poor. Investments in potato and sweetpotato breeding and allied disciplines at CIP have resulted in the release of many varieties some of which have had documented impact in the release countries. Partnership with diverse types of organizations has been key to the centers way of working toward improving livelihoods through crop production in the global South. [ABSTRACT FROM AUTHOR]

The transition from juvenile to adult stage is an important switch in the plant's life cycle. Potato is a temperate staple food crop consumed globally. Potato tuberization is influenced by various environmental factors, such as light and temperature. The complex signaling network comprising hormones, sugars, phloem mobile signals, and transcriptional/post-transcriptional regulators determine potato tuber yield. For instance, StBEL5 and StSP6A translocate from leaves to stolon to activate tuberization. Here, polypyrimidine tract-binding proteins and sugar transporters such as SUT and SWEET11 facilitate the translocation. Additionally, post-transcriptional regulation mediated by miRNAs and small RNAs control the expression of StSP6A under elevated temperatures. This review provides mechanistic insights into the signaling networks comprising transcriptional and post-transcriptional regulators that coordinate potato tuberization under photoperiod regimes and high-temperature conditions. Such signaling networks are under dynamic genetic and epigenetic regulation controlling potato tuberization. Also, the molecular dialogue between the source and sink tissues is highlighted. Such mechanistic information is crucial to develop climate-resilient cultivars to sustain potato productivity under changing climate. Together, genetic manipulation of these critical regulators by implementing modern biotechnological tools can be a promising approach to enhance tuber production in potato. [ABSTRACT FROM AUTHOR]

This study illustrates the salinity tolerance mechanisms in Volkameria inermis (a mangrove-associate), making it an ideal candidate for establishment in saline lands. The plant was exposed to 100, 200, 300, and 400 mM NaCl and the TI value indicates that the stress-imparting concentration was 400 mM. There was a decrease in biomass and tissue water, and a gradual increase in osmolytes like soluble sugars, proline, and free amino acids content was observed in plantlets with the increase in NaCl concentrations. Higher number of lignified cells in the vascular region of the plantlet's leaves treated with NaCl (400 mM) may influence the transport through the conducting tissues. SEM data reveals the presence of thick-walled xylem elements, an increased number of trichomes, and partially/fully closed stomata in the 400 mM NaCl-treated samples of V. inermis. In general, macro and micronutrient distribution tend to be affected in the NaCl-treated plantlets. However, Na content increased remarkably in plantlets treated with NaCl, and the highest accumulation was observed in roots (5.58-fold). Volkameria inermis can be a good option for phytodesalination in salt-affected areas since it is equipped with strong NaCl tolerance strategies and can be exploited for desalinization purpose of salt affected lands. The phytodesalination potential of V. inermis was proved with the aid of physiochemical and anatomical studies, which was not yet revealed. The present study elucidated the level of NaCl tolerance in V. inermis and the development of associated adaptive responses. [ABSTRACT FROM AUTHOR]

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Heat stress (HS) can affect growth performance through alterations in specific gut microbiota, which greatly threatens poultry production. How HS affects the mechanisms of microbial changes in the poultry cecum and the complex interactions between cecal microbial changes and growth performance have not yet been well evaluated. This study was conducted to examine the changes in growth performance and cecal microbiotal community in cyclic heat stress (CHS)–treated broilers. A total of 200 twenty-eight-day-old female Arbor Acres (AA) broilers were equally allotted into neutral ambient temperature group (TN group, 24 ± 1°C, 24 h/day) and CHS group (33 ± 1°C, 8 h/day) with five replicates of 10 broilers each, respectively. Growth performance, cecum microbial diversity, flora composition, and community structure were analyzed on days 35 and 42. The decreased average daily feed intake (ADFI), average daily gain (ADG), and the increased feed/gain ratio (F:G) were observed in heat-stressed broilers on days 35 and 42. The alpha and beta diversity index had no significant changes at the two experimental periods (P > 0.05). At the genus level, CHS significantly increased the relative abundance of Enterococcus at 42 days (P < 0.05). Based on the analysis of linear effect size feature selection, CHS made an enriched Reyranella and a reduced Romboutsia and Ruminiclostridium at 35 days of age (P < 0.05). CHS made an enriched Weissella and Enterococcus at 42 days of age (P < 0.05). The present study revealed that CHS reduces broiler growth performance and alters the microbial community of the cecum microbiota and the abundance of species. These findings are of critical importance to alleviate the negative effects of CHS on broiler chickens’ growth performance by maintaining gut microbial balance. [ABSTRACT FROM AUTHOR]

Sustainable agricultural practices help to manage and use natural resources efficiently. Due to global climate and geospatial land design, soil texture, soil–water content (SWC), and other parameters vary greatly; thus, real time, robust, and accurate soil analytical measurements are difficult to be developed. Conventional statistical analysis tools take longer to analyze and interpret data, which may have delayed a crucial decision. Therefore, this review paper is presented to develop the researcher's insight toward robust, accurate, and quick soil analysis using artificial intelligence (AI), deep learning (DL), and machine learning (ML) platforms to attain robustness in SWC and soil texture analysis. Machine learning algorithms, such as random forests, support vector machines, and neural networks, can be employed to develop predictive models based on available soil data and auxiliary environmental variables. Geostatistical techniques, including kriging and co-kriging, help interpolate and extrapolate soil property values to unsampled locations, improving the spatial representation of the data set. The false positivity in SWC results and bugs in advanced detection techniques are also evaluated, which may lead to wrong agricultural practices. Moreover, the advantages of AI data processing over general statistical analysis for robust and noise-free results have also been discussed in light of smart irrigation technologies. Conclusively, the conventional statistical tools for SWCs and soil texture analysis are not enough to practice and manage ergonomic land management. The broader geospatial non-numeric data are more suitable for AI processing that may soon help soil scientists develop a global SWC database. [ABSTRACT FROM AUTHOR]

Campylobacter infections and campylobacteriosis-associated post-infectious sequelae are a significant global health burden that needs to be addressed from a specific African perspective. We conducted a comprehensive literature search on NCBI PubMed to compile a comprehensive narrative review article on Campylobacter infections in Africa, focusing on key aspects in human and veterinary medicine as well as food hygiene. We specifically focused on the epidemiology of enteropathogenic Campylobacter spp. in sub-Saharan and North Africa considering antimicrobial susceptibility. The most significant sequela resulting from molecular mimicry to Campylobacter surface structures is the Guillain-Barré syndrome, which was mainly examined in the context of limited studies conducted in African populations. A dedicated subsection is allocated to the limited research on the veterinary medically important species Campylobacter fetus. There are significant differences in the composition of the gut microbiome, especially in rural areas, which affect the colonization with Campylobacter spp. and the manifestation of campylobacteriosis. There may be a problem of overdiagnosis due to asymptomatic colonization, particularly in the detection of Campylobacter using molecular biological techniques. To reduce the colonization and infection rate of Campylobacter, we propose implementing several control measures and urge further research to improve the current understanding of the peculiarities of campylobacteriosis in Africa. [ABSTRACT FROM AUTHOR]

This researchers focused at how adding vitamin C (VC) to biochar and replacing it with a mineral supplement affected egg quality and laying hen performance. 50 experimental units were created from 400 laying hens using a 5 × 2 factorial treatment design (10 treatments, 5 repeats, and 8 laying hens per repetition). Biochar levels (0, 25, 50, 75, and 100% replacement with mineral supplements of diet) and VC levels (0 and 100 mg/kg of diet) were some of the studied variables. The results showed that different experimental diets had no significant effect on performance parameters (feed intake, feed conversion ratio, daily weight gain, egg weight, egg production, and egg mass) of laying hens. In the whole of experiment (50–62 weeks of age), dietary treatments had no influence on egg albumen %, Haugh unit, albumen index, yolk %, yolk index, yolk color, egg shell thickness, or egg shell ash. The results revealed that biochar, due to its availability and easy production, can replace mineral supplements in laying hens' diet, with no adverse effects on productive performance and egg quality traits. [ABSTRACT FROM AUTHOR]

It is difficult to reach the water needed in agriculture. For this reason, insufficient irrigation causes yield and quality losses in plants. The aim of this study was to determine some quality parameters of both flesh of melon and melon seed irrigated with different irrigation regimes. Melon cultivar (Cucumis melo L.) was irrigated by the drip method from transplantation to the beginning of vegetative development, flowering-fruit binding, and fruit development-ripening. In this context, 8 irrigation groups were formed. Sugars, total phenolic compounds, titratable acidity, antioxidant activity and color analyzes were performed on flesh of melons. Oil content and fatty acid composition of melon seeds were also determined. The titratable acidity of samples varied from 0.098 to 0.159%. Irrigation regime had a significant effect on sucrose and total sugar content. Total phenolic compounds and antioxidant activities of samples were ranged from 80.6 to 244.8 (mg GAE/kg) and 0.17 to 0.38 (mmol TE/kg), respectively. The groups of non-irrigated and irrigated at vegetative-fruit development-ripening periods had the highest amount of total phenolic compounds and antioxidant activities. Among the color parameters, there were significant differences only in a* and h values. Although palmitic acid and oil content of seeds were not different, linoleic, oleic, and stearic acids were affected significantly by irrigation regime. As a result, in terms of sugar content, amount of phenolic compound and antioxidant activity, irrigation during VF period can be suggested for cultivation of melons in arid and semi-arid regions. [ABSTRACT FROM AUTHOR]

In order to investigate the effects of water stress and nitrogen fertilizer application on remobilization and grain yield of barley genotypes, two separate experiments were conducted at the Agricultural and Natural Resources Research Station of Miandoab during the years of 2014–2016 as a split plot based on randomized complete block design with three replications. The treatments included 5 genotypes and four nitrogen fertilizer levels (control or without fertilizer, 50, 100 and 150 kg ha−1 nitrogen (N) fertilizer). Under normal conditions, the maximum remobilization was obtained at 0 and 50 kg N application levels. N application increased non-significantly the remobilization under water deficit stress. The highest (1.22 g.m−2) and lowest (0.91 g.m−2) remobilization were recorded in 100 kg ha−1 N application and control. Bahman genotype, and Karoon and NK1272 genotypes had the highest remobilization under well irrigation and under water deficit, respectively. The highest remobilization to grain yield was related to 100 and 150 kg ha−1 N application. The comparison of N application levels showed that the highest current photosynthesis contribution from seed yield belongs to N application of 150 kg ha−1. Under water deficit, it was allocated to 50 kg ha−1. In conclusion, the greater grain yield in tolerant genotypes under water deficit was due to remobilization of unstructured carbohydrates from shoot to grain. Thus, it seems that selection of genotypes with higher translocated dry matter and contribution of assimilate in grain filling under water deficit, the suitable way for achieving cultivars with high grain yield under water deficit condition. [ABSTRACT FROM AUTHOR]

Salt stress causes plants to undergo metabolic and physiological disturbances, affecting growth, development, quality, and yield. With the increasing demand for food in the world and this environmental problem, the cultivation of salt-tolerant varieties or lines of crops is needed to compensate for the demands of food for mankind. This study was designed for salt tolerance problems; the four lines of Linum usitatissimum in terms of the plant's agronomic attributes, yield, different metabolites (secondary and primary), antioxidant enzymatic activities, endogenous hormones, lipid peroxidation and ion analysis. This research was completely randomized-design (CRD) and four lines of flax (Alsi ARI-50, Alsi ARI-22, Alsi ARI-20 and Alsi ARI-1) were raised using water (control) and 150 mM NaCl in irrigation water. Salinity had a negative impact on different growth parameters, photosynthetic pigments-level (Chlorophyll a, Chlorophyll b, total chlorophyll and chlorophyll a/b ratio), Indole-3-acetic acid (IAA), and different essential ions. On the other hand, at the same salt concentrations, carotenoid content, sugar level, total protein, lipids, phenolic content, proline amount, flavonoid, tannin, β carotene, antioxidant level, lipid peroxidation, catalase activity, guaiacol peroxidase activity, ascorbic acid, salicylic acid, sodium and chloride ions significantly increased. Based on the present study, it was concluded that Alsi ARI-50 and Alsi ARI-22 showed higher salt tolerance compared to the other lines based on measured parameters. So, these lines are recommended to be used in the salinity area and also in the breeding programme. [ABSTRACT FROM AUTHOR]

This study investigated the effect of locality and axial position on the morphological, physical, and mechanical properties of iron bamboo (Guadua angustifolia Kunt.) and solid bamboo [Dendrocalamus strictus (Roxb.) Nees] from three locations in the Philippines: Laguna, Baguio City, and Nueva Vizcaya. Morphological properties were assessed by measuring culm height, internode length (IL), culm diameter (CD), and culm wall thickness (CWT), whereas physical and mechanical properties were determined using ASTM D143-2019 and ISO 22157-2019. Results showed significant differences in properties based on localities and axial position. Laguna's iron bamboo had 75.29 and 11.87% higher CD and CWT than Baguio's, respectively. Laguna's solid bamboo CD exceeded Nueva Vizcaya's by 38.76%, but Nueva Vizcaya's solid bamboo had a 9.13% higher CWT than Laguna's. Iron bamboo's CD increased by 62.66%, and solid bamboo by 24.41% from bottom to top. Conversely, their CWTs decreased by 47.70 and 55.18%, respectively. Baguio City's iron bamboo exhibited greater relative density (RD), radial (RS), tangential (TS), longitudinal (LS), and volumetric shrinkage (VS) - surpassing Laguna's by 30.50, 42.58, 15.40, 33.96, and 29.31% respectively. However, its moisture content (MC) was 27.04% lower. Meanwhile, Laguna's solid bamboo had an 8.00% higher RD but an 18.46% lower MC compared to Nueva Vizcaya. Notably, solid bamboo from Nueva Vizcaya recorded the highest shrinkage properties. Both bamboo species showed an increasing trend in RD from the bottom to the top but a decreasing trend in MC, RS, TS, and VS. LS was highest in the top portion of iron bamboo and in the bottom portion of solid bamboo. Baguio City and Nueva Vizcaya bamboo exhibited the highest static bending strength than the bamboo from Laguna. In contrast, Laguna's culms showed significantly higher shear (SS) and maximum compression strength (MCS) - 46.09 and 97.09% higher for iron bamboo, and 100 and 123.29% higher for solid bamboo, respectively. The presence of nodes did not significantly affect SS and MCS in either species, but samples without nodes had higher strength than those with nodes. The result indicates that iron and solid bamboo from Laguna is suitable for construction and engineered bamboo applications, whereas bamboo from Baguio City and Nueva Vizcaya is recommended for structural applications that require high strength but not necessarily large culms. The findings highlight the versatility of Philippine-grown iron and solid bamboo as eco-friendly and sustainable raw materials for the bamboo industry in green building practices. [ABSTRACT FROM AUTHOR]

Gallibacterium anatis (G. anatis), a member of the Pasteurellaceae family, normally inhabits the upper respiratory and lower genital tracts of poultry. However, under certain circumstances of immunosuppression, co-infection (especially with Escherichia coli or Mycoplasma), or various stressors, G. anatis caused respiratory, reproductive, and systemic diseases. Infection with G. anatis has emerged in different countries worldwide. The bacterium affects mainly chickens; however, other species of domestic and wild birds may get infected. Horizontal, vertical, and venereal routes of G. anatis infection have been reported. The pathogenicity of G. anatis is principally related to the presence of some essential virulence factors such as Gallibacterium toxin A, fimbriae, haemagglutinin, outer membrane vesicles, capsule, biofilms, and protease. The clinical picture of G. anatis infection is mainly represented as tracheitis, oophoritis, salpingitis, and peritonitis, while other lesions may be noted in cases of concomitant infection. Control of such infection depends mainly on applying biosecurity measures and vaccination. The antimicrobial sensitivity test is necessary for the correct treatment of G. anatis. However, the development of multiple drug resistance is common. This review article sheds light on G. anatis regarding history, susceptibility, dissemination, virulence factors, pathogenesis, clinical picture, diagnosis, and control measures. [ABSTRACT FROM AUTHOR]