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1. SARS-CoV-2 in Animal Companions: A Serosurvey in Three Regions of Southern Italy

Author

Bianco, Angelica, primary, Bortolami, Alessio, additional, Miccolupo, Angela, additional, Sottili, Roldano, additional, Ghergo, Paola, additional, Castellana, Stefano, additional, Del Sambro, Laura, additional, Capozzi, Loredana, additional, Pagliari, Matteo, additional, Bonfante, Francesco, additional, Ridolfi, Donato, additional, Bulzacchelli, Carmela, additional, Giannico, Anna, additional, and Parisi, Antonio, additional

Published
2023
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2. High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

Author

Bianco, Angelica, primary, Normanno, Giovanni, additional, Capozzi, Loredana, additional, Del Sambro, Laura, additional, Di Fato, Laura, additional, Miccolupo, Angela, additional, Di Taranto, Pietro, additional, Caruso, Marta, additional, Petruzzi, Fiorenza, additional, Ali, Ashraf, additional, and Parisi, Antonio, additional

Published
2023
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3. Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods

Author

Mancini, Maria Emanuela, primary, Beverelli, Matteo, additional, Donatiello, Adelia, additional, Didonna, Antonella, additional, Dattoli, Luigi, additional, Faleo, Simona, additional, Occhiochiuso, Gilda, additional, Galante, Domenico, additional, Rondinone, Valeria, additional, Del Sambro, Laura, additional, Bianco, Angelica, additional, Miccolupo, Angela, additional, and Goffredo, Elisa, additional

Published
2022
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9. Large genetic diversity of Arcobacter butzleri isolated from raw milk in Southern Italy

Author

Miccolupo Angela, Mottola Anna, Di Pinto Angela, Parisi Antonio, Caruso Marta, Normanno Giovanni, Difato Laura, Santagada Gianfranco, Capozzi Loredana, and Bonerba Elisabetta

Subjects
Arcobacter, 0303 health sciences, Veterinary medicine, Genetic diversity, Food Safety, 030306 microbiology, Genetic Variation, Raw milk, Biology, biology.organism_classification, Microbiology, Arcobacter butzleri, 03 medical and health sciences, Milk, Italy, Food Microbiology, Multilocus sequence typing, Bulk tank, Animals, Typing, Genotyping, 030304 developmental biology, Food Science, Multilocus Sequence Typing
Abstract

Arcobacter butzleri is a zoonotic foodborne pathogen able to cause enteric and extraintestinal diseases. Its occurrence in foodstuff is well recognized worldwide but data on its presence in foods from Southern Italy are scarce. In this study the results on the occurrence and genotyping of Arcobacter spp. in bulk milk samples collected in Southern Italy are reported. Out of 484 samples, 64 (13.2%) resulted positive for the presence of Arcobacter spp. Using Real Time PCR but as few as 31.2% of these samples turned out as positive by using the cultural method, showing an overall prevalence of 4.1%. All isolates were identified as A. cryaerophilus using the biochemical identification whilst the sequencing of the atpA gene revealed that all the isolates were A. butzleri. Among the confirmed isolates, 16 different Sequence Types (ST) were identified using the Multi Locus Sequence Typing (MLST), 14 (87.5%) of which were previously unreported. Our survey reveals the presence of A. butzleri in bulk tank milk from Southern Italy and highlights the discrepancy between the two approaches used both for the detection (i.e., real time PCR vs cultural method) and the identification (i.e., biochemical test vs aptA sequencing) of Arcobacter spp In addition, a large genetic diversity among the isolates was detected and this makes the identification of source of the infections very challenging in outbreaks investigation.

Published
2019

10. MRSA in swine, farmers and abattoir workers in Southern Italy

Author

Parisi, Antonio, primary, Caruso, Marta, additional, Normanno, Giovanni, additional, Latorre, Laura, additional, Miccolupo, Angela, additional, Fraccalvieri, Rosa, additional, Intini, Francesco, additional, Manginelli, Teresa, additional, and Santagada, Gianfranco, additional

Published
2019
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12. Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products.

Author

Bianco, Angelica, Capozzi, Loredana, Miccolupo, Angela, Iannetti, Simona, Danzetta, Maria Luisa, Del Sambro, Laura, Caruso, Marta, Santagada, Gianfranco, and Parisi, Antonio

Subjects
BACILLUS cereus, DAIRY products, FOOD poisoning, GENES, PUBLIC safety
Abstract

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products. [ABSTRACT FROM AUTHOR]

Published
2020
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14. High Occurrence of Methicillin-ResistantStaphylococcus aureusin Horses at Slaughterhouses Compared with Those for Recreational Activities: A Professional and Food Safety Concern?

Author

Parisi, Antonio, primary, Caruso, Marta, additional, Normanno, Giovanni, additional, Latorre, Laura, additional, Miccolupo, Angela, additional, Fraccalvieri, Rosa, additional, Intini, Francesco, additional, Manginelli, Teresa, additional, and Santagada, Gianfranco, additional

Published
2017
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15. Antimicrobial Susceptibility and Multilocus Sequence Typing of Listeria monocytogenesIsolated Over 11 Years from Food, Humans, and the Environment in Italy

Author

Caruso, Marta, Fraccalvieri, Rosa, Pasquali, Frédérique, Santagada, Gianfranco, Latorre, Laura M., Difato, Laura M., Miccolupo, Angela, Normanno, Giovanni, and Parisi, Antonio

Abstract

Due to the increasing number of studies reporting the detection of antimicrobial-resistant isolates of Listeria monocytogenes, we sought to determine the antimicrobial susceptibility of L. monocytogenesisolates collected in Italy and find potential correlations to their serotypes and multilocus sequence types (MLST). The antimicrobial susceptibility of 317 L. monocytogenesisolates collected from food, humans, and the environment from 1998 to 2009 was assessed by minimum inhibitory concentration (MIC). Serotyping and MLST was also performed on all isolates. Potential correlations among antimicrobial resistance profiles, serotyping, and MLST were statistically evaluated. Twenty-four percent of L. monocytogenesisolates were resistant to oxacillin, 28.7% intermediate to clindamycin, and 24.3% to ciprofloxacin. The majority of isolates with elevated MIC to oxacillin was of environmental origin and belonged to serotype 4b/4e and ST2. Isolates with intermediate MIC values to clindamycin and ciprofloxacin were mostly of food and human origin and belonged to serotype 4b/4e and ST9. Regarding the time frame of isolate collection, comparing the last 3 years (2007–2009) to previous years (1998–2006), an increase was observed in the percentage of resistant and intermediate isolates per year. This trend strongly suggests the need for increasing attention on the prevalence of antimicrobial resistance in L. monocytogenesin Italy. To predict future resistance trends, the monitoring of clinical intermediate resistance might represent a useful tool especially for antibiotics associated to multiple-step mechanisms of acquired resistance. A specific focus should be addressed to antimicrobial-resistant isolates of serotype 4b, repeatedly associated with food-borne outbreaks.

Published
2020
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17. Arcobacter spp. in bovine milk: An emerging pathogen with potential zoonotic risk.

Author

Caruso, Marta, Latorre, Laura, Santagada, Gianfranco, Fraccalvieri, Rosa, Difato, Laura Maria, Miccolupo, Angela, Capozzi, Loredana, Bonerba, Elisabetta, Mottola, Anna, and Parisi, Antonio

Subjects
ARCOBACTER, MILK microbiology, ZOONOSES
Abstract

The aim of the present study was to assess the prevalence and genetic characteristics of Arcobacter spp. in bovine bulk tank milk produced in Apulia Region (Italy). Samples collected from 396 dairy farms, after enrichment in a selective broth, were subjected to an Arcobacter genus - specific Real Time PCR. Positive broths, previously filtered, were seeded on Karmali, MCCD and Columbia Blood Agar plates; presumptive Arcobacter spp. colonies were identified using an amplification and sequencing method and then characterized by Multi-Locus Sequence Typing (MLST). Prevalence of Arcobacter spp. in bovine milk samples was 5% (20/396); A. butzleri was the only isolated species, in agreement with previous studies that reported A. butzleri as the most commonly recovered species in milk and dairy products. MLST analysis of the 20 A. butzleri strains identified 81 alleles and 16 STs. Consistent with previous studies, MLST revealed a high level of heterogeneity between the A. butzleri isolates and confirmed the high discriminatory power of this method and its suitability for epidemiological investigations. This study confirmed the importance of raw milk as a possible source of Arcobacter spp. for humans. [ABSTRACT FROM AUTHOR]

Published
2018
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20. Comparative Genomics ofListeriaSensu Lato: Genus-Wide Differences in Evolutionary Dynamics and the Progressive Gain of Complex, Potentially Pathogenicity-Related Traits through Lateral Gene Transfer

Author

Chiara, Matteo, primary, Caruso, Marta, additional, D’Erchia, Anna Maria, additional, Manzari, Caterina, additional, Fraccalvieri, Rosa, additional, Goffredo, Elisa, additional, Latorre, Laura, additional, Miccolupo, Angela, additional, Padalino, Iolanda, additional, Santagada, Gianfranco, additional, Chiocco, Doriano, additional, Pesole, Graziano, additional, Horner, David S., additional, and Parisi, Antonio, additional

Published
2015
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22. High Occurrence of Methicillin-Resistant Staphylococcus aureusin Horses at Slaughterhouses Compared with Those for Recreational Activities: A Professional and Food Safety Concern?

Author

Parisi, Antonio, Caruso, Marta, Normanno, Giovanni, Latorre, Laura, Miccolupo, Angela, Fraccalvieri, Rosa, Intini, Francesco, Manginelli, Teresa, and Santagada, Gianfranco

Abstract

AbstractThe epidemiology of methicillin-resistant Staphylococcus aureus(MRSA) in horses and its zoonotic potential is poorly understood. The objective of this study is to provide data on the prevalence and genetic characteristics of MRSA isolated from horses on farms, at racecourses, and at slaughterhouses in Italy, using standard and molecular methods. In addition, we report the prevalence of MRSA in horse handlers. Among 388 horses tested by nasal swabs, 27 (7%) were positive for MRSA ST398 (t011, t899, t1255) and ST1 (t127). The prevalence of MRSA in horses tested at slaughterhouses was significantly higher (p< 0.001) compared with those tested on farms and racecourses. Five (7%) out of 67 staff members working in close contact with horses (2 from slaughterhouse, 2 from riding stable, and 1 from racecourse) were carriers of MRSA ST398 (t011, t034) and ST1 (t127). The isolates from horses and humans carried SCCmecIVa or V and were pvlnegative and piapositive. All the isolates from both horses and humans were resistant to at least two antimicrobial classes. The circulation of MRSA in horses and in humans working in close contact with them should be considered an emerging public health issue. In fact, it represents a potential risk for people who work in close contact with horses, and for horse meat consumers.

Published
2017
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25. Genetic analysis of canine parvovirus type 2c

Author

Decaro, Nicola, primary, Desario, Costantina, additional, Parisi, Antonio, additional, Martella, Vito, additional, Lorusso, Alessio, additional, Miccolupo, Angela, additional, Mari, Viviana, additional, Colaianni, Maria Loredana, additional, Cavalli, Alessandra, additional, Di Trani, Livia, additional, and Buonavoglia, Canio, additional

Published
2009
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26. Comparative Genomics of Listeria Sensu Lato: Genus-Wide Differences in Evolutionary Dynamics and the Progressive Gain of Complex, Potentially Pathogenicity-Related Traits through Lateral Gene Transfer.

Author

Chiara, Matteo, Caruso, Marta, D'Erchia, Anna Maria, Manzari, Caterina, Fraccalvieri, Rosa, Goffredo, Elisa, Latorre, Laura, Miccolupo, Angela, Padalino, Iolanda, Santagada, Gianfranco, Chiocco, Doriano, Pesole, Graziano, Horner, David S., and Parisi, Antonio

Subjects
GENOMES, LISTERIA, GENETIC transformation, MOLECULAR genetics, GRAM-positive bacteria
Abstract

Historically, genome-wide and molecular characterization of the genus Listeria has concentrated on the important human pathogen Listeriamonocytogenes and asmall number of closely related species, together termed Listeria sensu strictu.More recently, a number of genome sequences for more basal, and nonpathogenic, members of the Listeria genus have become available, facilitating a wider perspective on the evolution of pathogenicity and genome level evolutionary dynamics within the entire genus (termed Listeria sensu lato). Here, we have sequenced the genomes of additional Listeria fleischmannii and Listeria newyorkensis isolates and explored the dynamics of genome evolution in Listeria sensu lato. Our analyses suggest that acquisition of genetic material through gene duplication and divergence as well as through lateral gene transfer (mostly from outside Listeria) is widespread throughout the genus. Novel genetic material is apparently subject to rapid turnover. Multiple lines of evidence point to significant differences in evolutionary dynamics between themost basal Listeria subclade and all other congeners, including both sensu strictu and other sensu lato isolates. Strikingly, these differences are likely attributable to stochastic, population-level processes and contribute to observed variation in genome size across the genus. Notably, our analyses indicate that the common ancestor of Listeria sensu lato lacked flagella, whichwere acquired by lateral gene transfer by a common ancestor of Listeria grayi and Listeria sensu strictu, whereas a recently functionally characterized pathogenicity island, responsible for the capacity to produce cobalamin and utilize ethanolamine/propane-2-diol, was acquired in an ancestor of Listeria sensu strictu. [ABSTRACT FROM AUTHOR]

Published
2015
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27. Comparison between Salmonella entericaSerotype Enteritidis Genotyping Methods and Phage Type

Author

De Cesare, Alessandra, Krishnamani, Keshav, Parisi, Antonio, Ricci, Antonia, Luzzi, Ida, Barco, Lisa, Lucchi, Alex, Miccolupo, Angela, and Manfreda, Gerardo

Abstract

ABSTRACTA quantitative comparison between discriminatory indexes and concordance among multilocus variable-number tandem-repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and phage typing has been performed, testing 238 Salmonella entericaserotype Enteritidis isolates not epidemiologically correlated. The results show that MLVA is the best choice, but each typing method provides a piece of information for establishing clonal relationships between the isolates.

Published
2015
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