194 results on '"Mabire M"'
Search Results
2. Monoacylglycerol lipase reprograms hepatocytes and macrophages to promote liver regeneration.
- Author
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Allaire M, Al Sayegh R, Mabire M, Hammoutene A, Siebert M, Caër C, Cadoux M, Wan J, Habib A, Le Gall M, de la Grange P, Guillou H, Postic C, Paradis V, Lotersztajn S, and Gilgenkrantz H
- Abstract
Background & Aims: Liver regeneration is a repair process in which metabolic reprogramming of parenchymal and inflammatory cells plays a major role. Monoacylglycerol lipase (MAGL) is an ubiquitous enzyme at the crossroad between lipid metabolism and inflammation. It converts monoacylglycerols into free fatty acids and metabolises 2-arachidonoylglycerol into arachidonic acid, being thus the major source of pro-inflammatory prostaglandins in the liver. In this study, we investigated the role of MAGL in liver regeneration., Methods: Hepatocyte proliferation was studied in vitro in hepatoma cell lines and ex vivo in precision-cut human liver slices. Liver regeneration was investigated in mice treated with a pharmacological MAGL inhibitor, MJN110, as well as in animals globally invalidated for MAGL (MAGL
-/- ) and specifically invalidated in hepatocytes (MAGLHep-/- ) or myeloid cells (MAGLMye-/- ). Two models of liver regeneration were used: acute toxic carbon tetrachloride injection and two-thirds partial hepatectomy. MAGLMye-/- liver macrophages profiling was analysed by RNA sequencing. A rescue experiment was performed by in vivo administration of interferon receptor antibody in MAGLMye-/- mice., Results: Precision-cut human liver slices from patients with chronic liver disease and human hepatocyte cell lines exposed to MJN110 showed reduced hepatocyte proliferation. Mice with global invalidation or mice treated with MJN110 showed blunted liver regeneration. Moreover, mice with specific deletion of MAGL in either hepatocytes or myeloid cells displayed delayed liver regeneration. Mechanistically, MAGLHep-/- mice showed reduced liver eicosanoid production, in particular prostaglandin E2 that negatively impacts on hepatocyte proliferation. MAGL inhibition in macrophages resulted in the induction of the type I interferon pathway. Importantly, neutralising the type I interferon pathway restored liver regeneration of MAGLMye-/- mice., Conclusions: Our data demonstrate that MAGL promotes liver regeneration by hepatocyte and macrophage reprogramming., Impact and Implications: By using human liver samples and mouse models of global or specific cell type invalidation, we show that the monoacylglycerol pathway plays an essential role in liver regeneration. We unveil the mechanisms by which MAGL expressed in both hepatocytes and macrophages impacts the liver regeneration process, via eicosanoid production by hepatocytes and the modulation of the macrophage interferon pathway profile that restrains hepatocyte proliferation., (© 2023 The Author(s).)- Published
- 2023
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3. MAIT cell inhibition promotes liver fibrosis regression via macrophage phenotype reprogramming.
- Author
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Mabire M, Hegde P, Hammoutene A, Wan J, Caër C, Sayegh RA, Cadoux M, Allaire M, Weiss E, Thibault-Sogorb T, Lantz O, Goodhardt M, Paradis V, de la Grange P, Gilgenkrantz H, and Lotersztajn S
- Subjects
- Humans, Male, Mice, Animals, Liver Cirrhosis pathology, Macrophages, Liver pathology, Fibrosis, Phenotype, Mice, Inbred C57BL, Mucosal-Associated Invariant T Cells, Non-alcoholic Fatty Liver Disease pathology
- Abstract
Recent data have shown that liver fibrosis can regress even at later stages of cirrhosis and shifting the immune response from pro-inflammatory towards a resolutive profile is considered as a promising option. The immune regulatory networks that govern the shift of the inflammatory phenotype and thus potential reversal of liver fibrosis are lesser known. Here we show that in precision-cut human liver slices obtained from patients with end-stage fibrosis and in mouse models, inhibiting Mucosal-Associated Invariant T (MAIT) cells using pharmacological or antibody-driven approaches, limits fibrosis progression and even regresses fibrosis, following chronic toxic- or non-alcoholic steatohepatitis (NASH)-induced liver injury. Mechanistic studies, combining RNA sequencing, in vivo functional studies (performed in male mice) and co-culture experiments indicate that disruption of the MAIT cell-monocyte/macrophage interaction results in resolution of fibrosis both by increasing the frequency of restorative Ly6C
lo at the expenses of pro-fibrogenic Ly6Chi monocyte-derived macrophages and promoting an autophagic phenotype in both subsets. Thus, our data show that MAIT cell activation and the consequential phenotype shift of liver macrophages are important pathogenic features of liver fibrosis and could be targeted by anti-fibrogenic therapy., (© 2023. The Author(s).)- Published
- 2023
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4. Functional relevance and pro-fibrogenic properties of mucosal-associated invariant T cells (MAIT) during chronic liver diseases
- Author
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Hegde, P., primary, Weiss, E., additional, Paradis, Valérie, additional, Wan, J., additional, Mabire, M., additional, Sukriti, S., additional, Rautou, P.-E., additional, Albuquerque, M., additional, Picq, O., additional, Gupta, A., additional, Ferrere, G., additional, Gilgenkrantz, Hélène, additional, Kiaf, B., additional, Toubal, A., additional, Beaudoin, L., additional, Letteron, P., additional, Moreau, R., additional, Lehuen, A., additional, and Lotersztajn, S., additional
- Published
- 2018
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5. Mucosal-associated invariant T cells are functionally impaired during chronic liver diseases and promote fibrosis
- Author
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Hegde, P., primary, Weiss, E., additional, Wan, J., additional, Paradis, V., additional, Rautou, P.-E., additional, Mabire, M., additional, Ferrere, G., additional, Gupta, A., additional, Letteron, P., additional, Kiaf, B., additional, Toubal, A., additional, Moreau, R., additional, Lehuen, A., additional, and Lotersztajn, S., additional
- Published
- 2017
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6. LC3-associated phagocytosis in myeloid cells, a fireman that restrains inflammation and liver fibrosis, via immunoreceptor inhibitory signaling.
- Author
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Wan J, Weiss E, Ben Mkaddem S, Mabire M, Choinier PM, Thibault-Sogorb T, Hegde P, Bens M, Broer L, Gilgenkrantz H, Moreau R, Saveanu L, Codogno P, Monteiro RC, and Lotersztajn S
- Subjects
- Humans, Inflammation blood, Liver Cirrhosis blood, Inflammation pathology, Liver Cirrhosis pathology, Microtubule-Associated Proteins metabolism, Myeloid Cells metabolism, Myeloid Cells pathology, Phagocytosis, Signal Transduction
- Abstract
Control of systemic and hepatic inflammation, in particular originating from monocytes/macrophages, is crucial to prevent liver fibrosis and its progression to end-stage cirrhosis. LC3-associated phagocytosis (LAP) is a non-canonical form of autophagy that shifts the monocyte/macrophage phenotype to an anti-inflammatory phenotype. In a recent study, we uncovered LAP as a protective mechanism against inflammation-driven liver fibrosis and systemic inflammation in the context of cirrhosis. We observed that LAP is enhanced in blood and liver monocytes from patients with liver fibrosis or those who progress to cirrhosis. Combining studies in which LAP was pharmacologically or genetically inactivated, we found that LAP limits inflammation in monocytes from cirrhotic patients, and the hepatic inflammatory profile in mice with chronic liver injury, resulting in anti-fibrogenic effects. Mechanistically, LAP-induced anti-inflammatory and antifibrogenic signaling results from enhanced expression of the Fc immunoreceptor FCGR2A/FcγRIIA and activation of an FCGR2A-mediated PTPN6/SHP-1 anti-inflammatory pathway, leading to increased engulfment of IgG into LC3
+ phagosomes. In patients with cirrhosis progressing to multi-organ failure (acute-on chronic liver failure), LAP is lost in monocytes, and can be restored by targeting FCGR2A-mediated PTPN6/SHP-1 signaling. These data suggest that sustaining LAP may open novel therapeutic perspectives for patients with end-stage liver disease.- Published
- 2020
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7. LC3-associated phagocytosis protects against inflammation and liver fibrosis via immunoreceptor inhibitory signaling.
- Author
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Wan J, Weiss E, Ben Mkaddem S, Mabire M, Choinier PM, Picq O, Thibault-Sogorb T, Hegde P, Pishvaie D, Bens M, Broer L, Gilgenkrantz H, Moreau R, Saveanu L, Codogno P, Monteiro RC, and Lotersztajn S
- Subjects
- Animals, Humans, Inflammation, Mice, Mice, Inbred C57BL, Microtubule-Associated Proteins, Liver Cirrhosis, Phagocytosis, Signal Transduction
- Abstract
Sustained hepatic and systemic inflammation, particularly originating from monocytes/macrophages, is a driving force for fibrosis progression to end-stage cirrhosis and underlies the development of multiorgan failure. Reprogramming monocyte/macrophage phenotype has emerged as a strategy to limit inflammation during chronic liver injury. Here, we report that LC3-associated phagocytosis (LAP), a noncanonical form of autophagy, protects against hepatic and systemic inflammation during chronic liver injury in rodents, with beneficial antifibrogenic effects. LAP is enhanced in blood and liver monocytes from patients with fibrosis and cirrhosis. Pharmacological inhibition of LAP components in human monocytes from patients with cirrhosis or genetic disruption of LAP in mice with chronic liver injury exacerbates both the inflammatory signature in isolated human monocytes and the hepatic inflammatory profile in mice, resulting in enhanced liver fibrosis. Mechanistically, patients with cirrhosis showed increased monocyte expression of Fc fragment of IgG receptor IIA (FcγRIIA) and enhanced engulfment of immunoglobulin G in LC3
+ phagosomes that triggers an FcγRIIA/Src homology region 2 domain-containing phosphatase-1 (SHP-1) inhibitory immunoreceptor tyrosine-based activation motif (ITAMi) anti-inflammatory pathway. Mice overexpressing human FcγRIIA in myeloid cells show enhanced LAP in response to chronic liver injury and resistance to inflammation and liver fibrosis. Activation of LAP is lost in monocytes from patients with multiorgan failure and restored by specifically targeting ITAMi signaling with anti-FcγRIIA F(ab')2 fragments, or with intravenous immunoglobulin (IVIg). These data suggest the existence of an ITAMi-mediated mechanism by which LAP might protect against inflammation. Sustaining LAP may open therapeutic perspectives for patients with chronic liver disease., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)- Published
- 2020
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8. FRI-063 - Functional relevance and pro-fibrogenic properties of mucosal-associated invariant T cells (MAIT) during chronic liver diseases
- Author
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Hegde, P., Weiss, E., Paradis, Valérie, Wan, J., Mabire, M., Sukriti, S., Rautou, P.-E., Albuquerque, M., Picq, O., Gupta, A., Ferrere, G., Gilgenkrantz, Hélène, Kiaf, B., Toubal, A., Beaudoin, L., Letteron, P., Moreau, R., Lehuen, A., and Lotersztajn, S.
- Published
- 2018
- Full Text
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9. Inhibition of monoacylglycerol lipase, an anti-inflammatory and antifibrogenic strategy in the liver.
- Author
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Habib A, Chokr D, Wan J, Hegde P, Mabire M, Siebert M, Ribeiro-Parenti L, Le Gall M, Lettéron P, Pilard N, Mansouri A, Brouillet A, Tardelli M, Weiss E, Le Faouder P, Guillou H, Cravatt BF, Moreau R, Trauner M, and Lotersztajn S
- Subjects
- Animals, Anti-Inflammatory Agents pharmacology, Autophagy drug effects, Carbamates pharmacology, Carbamates therapeutic use, Carbon Tetrachloride, Cell Count, Cells, Cultured, Cytokines metabolism, Disease Progression, Drug Evaluation, Preclinical methods, Hydrolases metabolism, Inflammation Mediators antagonists & inhibitors, Inflammation Mediators metabolism, Liver Cirrhosis, Experimental chemically induced, Liver Cirrhosis, Experimental enzymology, Liver Cirrhosis, Experimental pathology, Macrophages drug effects, Macrophages metabolism, Macrophages physiology, Male, Mice, Inbred C57BL, Mice, Knockout, Molecular Targeted Therapy methods, Monoacylglycerol Lipases physiology, Receptor, Cannabinoid, CB2 metabolism, Succinimides pharmacology, Succinimides therapeutic use, Anti-Inflammatory Agents therapeutic use, Liver enzymology, Liver Cirrhosis, Experimental drug therapy, Monoacylglycerol Lipases antagonists & inhibitors
- Abstract
Objective: Sustained inflammation originating from macrophages is a driving force of fibrosis progression and resolution. Monoacylglycerol lipase (MAGL) is the rate-limiting enzyme in the degradation of monoacylglycerols. It is a proinflammatory enzyme that metabolises 2-arachidonoylglycerol, an endocannabinoid receptor ligand, into arachidonic acid. Here, we investigated the impact of MAGL on inflammation and fibrosis during chronic liver injury., Design: C57BL/6J mice and mice with global invalidation of MAGL (MAGL
-/- ), or myeloid-specific deletion of either MAGL (MAGLMye-/- ), ATG5 (ATGMye-/- ) or CB2 (CB2Mye-/- ), were used. Fibrosis was induced by repeated carbon tetrachloride (CCl4 ) injections or bile duct ligation (BDL). Studies were performed on peritoneal or bone marrow-derived macrophages and Kupffer cells., Results: MAGL-/- or MAGLMye-/- mice exposed to CCl4 or subjected to BDL were more resistant to inflammation and fibrosis than wild-type counterparts. Therapeutic intervention with MJN110, an MAGL inhibitor, reduced hepatic macrophage number and inflammatory gene expression and slowed down fibrosis progression. MAGL inhibitors also accelerated fibrosis regression and increased Ly-6Clow macrophage number. Antifibrogenic effects exclusively relied on MAGL inhibition in macrophages, since MJN110 treatment of MAGLMye-/- BDL mice did not further decrease liver fibrosis. Cultured macrophages exposed to MJN110 or from MAGLMye-/- mice displayed reduced cytokine secretion. These effects were independent of the cannabinoid receptor 2, as they were preserved in CB2Mye-/- mice. They relied on macrophage autophagy, since anti-inflammatory and antifibrogenic effects of MJN110 were lost in ATG5Mye-/- BDL mice, and were associated with increased autophagic flux and autophagosome biosynthesis in macrophages when MAGL was pharmacologically or genetically inhibited., Conclusion: MAGL is an immunometabolic target in the liver. MAGL inhibitors may show promising antifibrogenic effects during chronic liver injury., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.)- Published
- 2019
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10. Mucosal-associated invariant T cells are a profibrogenic immune cell population in the liver.
- Author
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Hegde P, Weiss E, Paradis V, Wan J, Mabire M, Sukriti S, Rautou PE, Albuquerque M, Picq O, Gupta AC, Ferrere G, Gilgenkrantz H, Kiaf B, Toubal A, Beaudoin L, Lettéron P, Moreau R, Lehuen A, and Lotersztajn S
- Subjects
- Adult, Aged, Animals, Cell Count, Cells, Cultured, Coculture Techniques, Female, Humans, Liver immunology, Liver pathology, Liver Cirrhosis blood, Liver Cirrhosis pathology, Male, Mice, Middle Aged, Non-alcoholic Fatty Liver Disease blood, Non-alcoholic Fatty Liver Disease pathology, Liver Cirrhosis immunology, Macrophages immunology, Mucosal-Associated Invariant T Cells immunology, Non-alcoholic Fatty Liver Disease immunology
- Abstract
Liver fibrosis is the common response to chronic liver injury, and leads to cirrhosis and its complications. Persistent inflammation is a driving force of liver fibrosis progression. Mucosal-associated invariant T (MAIT) cells are non-conventional T cells that display altered functions during chronic inflammatory diseases. Here, we show that circulating MAIT cells are reduced in patients with alcoholic or non-alcoholic fatty liver disease-related cirrhosis while they accumulate in liver fibrotic septa. Using two models of chronic liver injury, we demonstrate that MAIT cell-enriched mice show increased liver fibrosis and accumulation of hepatic fibrogenic cells, whereas MAIT cell-deficient mice are resistant. Co-culture experiments indicate that MAIT cells enhance the proinflammatory properties of monocyte-derived macrophages, and promote mitogenic and proinflammatory functions of fibrogenic cells, via distinct mechanisms. Our results highlight the profibrogenic functions of MAIT cells and suggest that targeting MAIT cells may constitute an attractive antifibrogenic strategy during chronic liver injury.
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- 2018
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11. Role of the type 3 cytokines IL-17 and IL-22 in modulating metabolic dysfunctionassociated steatotic liver disease.
- Author
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Abdelnabi, Mohamed N., Hassan, Ghada S., and Shoukry, Naglaa H.
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HEPATIC fibrosis ,MYELOID cells ,LIVER cells ,INNATE lymphoid cells ,T cells - Abstract
Metabolic dysfunction-associated steatotic liver disease (MASLD) comprises a spectrum of liver diseases that span simple steatosis, metabolic dysfunctionassociated steatohepatitis (MASH) and fibrosis and may progress to cirrhosis and cancer. The pathogenesis of MASLD is multifactorial and is driven by environmental, genetic, metabolic and immune factors. This review will focus on the role of the type 3 cytokines IL-17 and IL-22 in MASLD pathogenesis and progression. IL-17 and IL-22 are produced by similar adaptive and innate immune cells such as Th17 and innate lymphoid cells, respectively. IL-17-related signaling is upregulated during MASLD resulting in increased chemokines and proinflammatory cytokines in the liver microenvironment, enhanced recruitment of myeloid cells and T cells leading to exacerbation of inflammation and liver disease progression. IL-17 may also act directly by activating hepatic stellate cells resulting in increased fibrosis. In contrast, IL-22 is a pleiotropic cytokine with a dominantly protective signature in MASLD and is currently being tested as a therapeutic strategy. IL-22 also exhibits beneficial metabolic effects and abrogates MASH-related inflammation and fibrosis development via inducing the production of anti-oxidants and anti-apoptotic factors. A sex-dependent effect has been attributed to both cytokines, most importantly to IL-22 in MASLD or related conditions. Altogether, IL-17 and IL-22 are key effectors in MASLD pathogenesis and progression. We will review the role of these two cytokines and cells that produce them in the development of MASLD, their interaction with host factors driving MASLD including sexual dimorphism, and their potential therapeutic benefits. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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12. Advancements of non‐invasive imaging technologies for the diagnosis and staging of liver fibrosis: Present and future.
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Huang, Wenpeng, Peng, Yushuo, and Kang, Lei
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HEPATIC fibrosis ,SINGLE-photon emission computed tomography ,MAGNETIC resonance imaging ,DIAGNOSTIC ultrasonic imaging - Abstract
Liver fibrosis is a reparative response triggered by liver injury. Non‐invasive assessment and staging of liver fibrosis in patients with chronic liver disease are of paramount importance, as treatment strategies and prognoses depend significantly on the degree of fibrosis. Although liver fibrosis has traditionally been staged through invasive liver biopsy, this method is prone to sampling errors, particularly when biopsy sizes are inadequate. Consequently, there is an urgent clinical need for an alternative to biopsy, one that ensures precise, sensitive, and non‐invasive diagnosis and staging of liver fibrosis. Non‐invasive imaging assessments have assumed a pivotal role in clinical practice, enjoying growing popularity and acceptance due to their potential for diagnosing, staging, and monitoring liver fibrosis. In this comprehensive review, we first delved into the current landscape of non‐invasive imaging technologies, assessing their accuracy and the transformative impact they have had on the diagnosis and management of liver fibrosis in both clinical practice and animal models. Additionally, we provided an in‐depth exploration of recent advancements in ultrasound imaging, computed tomography imaging, magnetic resonance imaging, nuclear medicine imaging, radiomics, and artificial intelligence within the field of liver fibrosis research. We summarized the key concepts, advantages, limitations, and diagnostic performance of each technique. Finally, we discussed the challenges associated with clinical implementation and offer our perspective on advancing the field, hoping to provide alternative directions for the future research. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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13. Lipid homeostasis in diabetic kidney disease.
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Ying Wang, Tongtong Liu, Yun Wu, Lin Wang, Shaowei Ding, Baoluo Hou, Hailing Zhao, Weijing Liu, and Ping Li
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- 2024
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14. Liver Fibrosis: From Basic Science towards Clinical Progress, Focusing on the Central Role of Hepatic Stellate Cells.
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Akkız, Hikmet, Gieseler, Robert K., and Canbay, Ali
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HEPATIC fibrosis ,LIVER cells ,HEPATITIS ,EXTRACELLULAR matrix ,VITAMIN A ,CELL death ,CHEMOKINE receptors - Abstract
The burden of chronic liver disease is globally increasing at an alarming rate. Chronic liver injury leads to liver inflammation and fibrosis (LF) as critical determinants of long-term outcomes such as cirrhosis, liver cancer, and mortality. LF is a wound-healing process characterized by excessive deposition of extracellular matrix (ECM) proteins due to the activation of hepatic stellate cells (HSCs). In the healthy liver, quiescent HSCs metabolize and store retinoids. Upon fibrogenic activation, quiescent HSCs transdifferentiate into myofibroblasts; lose their vitamin A; upregulate α-smooth muscle actin; and produce proinflammatory soluble mediators, collagens, and inhibitors of ECM degradation. Activated HSCs are the main effector cells during hepatic fibrogenesis. In addition, the accumulation and activation of profibrogenic macrophages in response to hepatocyte death play a critical role in the initiation of HSC activation and survival. The main source of myofibroblasts is resident HSCs. Activated HSCs migrate to the site of active fibrogenesis to initiate the formation of a fibrous scar. Single-cell technologies revealed that quiescent HSCs are highly homogenous, while activated HSCs/myofibroblasts are much more heterogeneous. The complex process of inflammation results from the response of various hepatic cells to hepatocellular death and inflammatory signals related to intrahepatic injury pathways or extrahepatic mediators. Inflammatory processes modulate fibrogenesis by activating HSCs and, in turn, drive immune mechanisms via cytokines and chemokines. Increasing evidence also suggests that cellular stress responses contribute to fibrogenesis. Recent data demonstrated that LF can revert even at advanced stages of cirrhosis if the underlying cause is eliminated, which inhibits the inflammatory and profibrogenic cells. However, despite numerous clinical studies on plausible drug candidates, an approved antifibrotic therapy still remains elusive. This state-of-the-art review presents cellular and molecular mechanisms involved in hepatic fibrogenesis and its resolution, as well as comprehensively discusses the drivers linking liver injury to chronic liver inflammation and LF. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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15. The Impact of the CB 2 Cannabinoid Receptor in Inflammatory Diseases: An Update.
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Rakotoarivelo, Volatiana, Mayer, Thomas Z., Simard, Mélissa, Flamand, Nicolas, and Di Marzo, Vincenzo
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CELL receptors ,ONLINE databases ,CELL migration ,MODERN society ,RHEUMATISM ,CANNABINOID receptors - Abstract
The emergence of inflammatory diseases is a heavy burden on modern societies. Cannabis has been used for several millennia to treat inflammatory disorders such as rheumatism or gout. Since the characterization of cannabinoid receptors, CB
1 and CB2 , the potential of cannabinoid pharmacotherapy in inflammatory conditions has received great interest. Several studies have identified the importance of these receptors in immune cell migration and in the production of inflammatory mediators. As the presence of the CB2 receptor was documented to be more predominant in immune cells, several pharmacological agonists and antagonists have been designed to treat inflammation. To better define the potential of the CB2 receptor, three online databases, PubMed, Google Scholar and clinicaltrial.gov, were searched without language restriction. The full texts of articles presenting data on the endocannabinoid system, the CB2 receptor and its role in modulating inflammation in vitro, in animal models and in the context of clinical trials were reviewed. Finally, we discuss the clinical potential of the latest cannabinoid-based therapies in inflammatory diseases. [ABSTRACT FROM AUTHOR]- Published
- 2024
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16. Generation, Characterisation and Identification of Bioactive Peptides from Mesopelagic Fish Protein Hydrolysates Using In Silico and In Vitro Approaches.
- Author
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Hayes, Maria, Naik, Azza, Mora, Leticia, Iñarra, Bruno, Ibarruri, Jone, Bald, Carlos, Cariou, Thibault, Reid, David, Gallagher, Michael, Dragøy, Ragnhild, Galino, Jorge, Deyà, Alba, Albrektsen, Sissel, Thoresen, Lars, and Solstad, Runar G.
- Abstract
This study generated bioactive hydrolysates using the enzyme Alcalase and autolysis from mesopelagic fish, including Maurolicus muelleri and Benthosema glaciale. Generated hydrolysates were investigated for their bioactivities using in vitro bioassays, and bioactive peptides were identified using mass spectrometry in active hydrolysates with cyclooxygenase, dipeptidyl peptidase IV and antioxidant activities. In silico analysis was employed to rank identified peptide sequences in terms of overall bioactivity using programmes including Peptide Ranker, PrepAIP, Umami-MRNN and AntiDMPpred. Seven peptides predicted to have anti-inflammatory, anti-type 2 diabetes or Umami potential using in silico strategies were chemically synthesised, and their anti-inflammatory activities were confirmed using in vitro bioassays with COX-1 and COX-2 enzymes. The peptide QCPLHRPWAL inhibited COX-1 and COX-2 by 82.90% (+/−0.54) and 53.84%, respectively, and had a selectivity index greater than 10. This peptide warrants further research as a novel anti-inflammatory/pain relief peptide. Other peptides with DPP-IV inhibitory and Umami flavours were identified. These offer potential for use as functional foods or topical agents to prevent pain and inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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17. Cell metabolism-based therapy for liver fibrosis, repair, and hepatocellular carcinoma.
- Author
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Gilgenkrantz H, Paradis V, and Lotersztajn S
- Abstract
Progression of chronic liver injury to fibrosis, abnormal liver regeneration, and HCC is driven by a dysregulated dialog between epithelial cells and their microenvironment, in particular immune, fibroblasts, and endothelial cells. There is currently no antifibrogenic therapy, and drug treatment of HCC is limited to tyrosine kinase inhibitors and immunotherapy targeting the tumor microenvironment. Metabolic reprogramming of epithelial and nonparenchymal cells is critical at each stage of disease progression, suggesting that targeting specific metabolic pathways could constitute an interesting therapeutic approach. In this review, we discuss how modulating intrinsic metabolism of key effector liver cells might disrupt the pathogenic sequence from chronic liver injury to fibrosis/cirrhosis, regeneration, and HCC., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2023
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18. Regulation of MAIT cells through host-derived antigens.
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Emi Ito and Sho Yamasaki
- Abstract
Mucosal-associated invariant T (MAIT) cells are a major subset of innate-like T cells that function at the interface between innate and acquired immunity. MAIT cells recognize vitamin B2-related metabolites produced by microbes, through semi-invariant T cell receptor (TCR) and contribute to protective immunity. These foreign-derived antigens are presented by a monomorphic antigen presenting molecule, MHC class I-related molecule 1 (MR1). MR1 contains a malleable ligand-binding pocket, allowing for the recognition of compounds with various structures. However, interactions between MR1 and self-derived antigens are not fully understood. Recently, bile acid metabolites were identified as host-derived ligands for MAIT cells. In this review, we will highlight recent findings regarding the recognition of self-antigens by MAIT cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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19. A review of MASLD-related hepatocellular carcinoma: progress in pathogenesis, early detection, and therapeutic interventions.
- Author
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Yang Ma, Jinguo Wang, Wenping Xiao, and Xiaoming Fan
- Published
- 2024
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20. Macrophage Perspectives in Liver Diseases: Programmed Death, Related Biomarkers, and Targeted Therapy.
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Qian, Zibing, Xiong, Wanyuan, Mao, Xiaorong, and Li, Junfeng
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LIVER diseases ,MACROPHAGES ,BIOMARKERS ,CELL death ,PHENOTYPIC plasticity ,HOMEOSTASIS - Abstract
Macrophages, as important immune cells of the organism, are involved in maintaining intrahepatic microenvironmental homeostasis and can undergo rapid phenotypic changes in the injured or recovering liver. In recent years, the crucial role of macrophage-programmed cell death in the development and regression of liver diseases has become a research hotspot. Moreover, macrophage-targeted therapeutic strategies are emerging in both preclinical and clinical studies. Given the macrophages' vital role in complex organismal environments, there is tremendous academic interest in developing novel therapeutic strategies that target these cells. This review provides an overview of the characteristics and interactions between macrophage polarization, programmed cell death, related biomarkers, and macrophage-targeted therapies. It aims to deepen the understanding of macrophage immunomodulation and molecular mechanisms and to provide a basis for the treatment of macrophage-associated liver diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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21. Can Nutraceuticals Support the Treatment of MASLD/MASH, and thus Affect the Process of Liver Fibrosis?
- Author
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Sokal-Dembowska, Aneta, Jarmakiewicz-Czaja, Sara, Ferenc, Katarzyna, and Filip, Rafał
- Subjects
HEPATIC fibrosis ,OMEGA-3 fatty acids ,VITAMIN C ,FATTY liver ,LIVER diseases ,BEHAVIOR therapy - Abstract
Currently, metabolic dysfunction-associated steatotic liver disease (MASLD) and metabolic dysfunction-associated steatohepatitis (MASH) are considered to be the main causes of fibrosis. In turn, fibrosis may lead to the development of hepatocellular carcinoma or advanced cirrhosis, i.e., potentially life-threatening conditions. It is likely that therapy aimed at reducing the risk of developing hepatic steatosis and inflammation could be helpful in minimizing the threat/probability of organ fibrosis. In recent years, increasing attention has been paid to the influence of nutraceuticals in the prevention and treatment of liver diseases. Therefore, the aim of this review was to describe the precise role of selected ingredients such as vitamin C, beta-carotene, omega-3 fatty acids, and curcumin. It is likely that the use of these ingredients in the treatment of patients with MASLD/MASH, along with behavioral and pharmacological therapy, may have a beneficial effect on combating inflammation, reducing oxidative stress, and thereby preventing liver damage. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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22. Nicotinamide Supplementation Mitigates Oxidative Injury of Bovine Intestinal Epithelial Cells through Autophagy Modulation.
- Author
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Guo, Yihan, Feng, Changdong, Zhang, Yiwei, Hu, Kewei, Wang, Chong, and Wei, Xiaoshi
- Subjects
NICOTINAMIDE ,EPITHELIAL cells ,INTESTINAL injuries ,OXIDANT status ,TIGHT junctions ,AUTOPHAGY - Abstract
Simple Summary: The small intestine of ruminants is an important organ in the digestion and absorption of rumen indigestible nutrients while it is prone to oxidative stress, causing barrier damage, thus intestinal absorption and metabolic disorders. Nicotinamide was previously found to have antioxidant properties. The aims of this study were to investigate the effect of nicotinamide on intestinal oxidative damage and explore its potential mechanism. The study revealed that nicotinamide effectively reduced oxidative damage in bovine intestinal epithelial cells through autophagy. This protective effect was evidenced by enhanced antioxidant capacity and increased expression of tight junction proteins, suggesting nicotinamide's role in improving cellular defense and intestinal barrier integrity. The small intestine is important to the digestion and absorption of rumen undegradable nutrients, as well as the barrier functionality and immunological responses in ruminants. Oxidative stress induces a spectrum of pathophysiological symptoms and nutritional deficits, causing various gastrointestinal ailments. Previous studies have shown that nicotinamide (NAM) has antioxidant properties, but the potential mechanism has not been elucidated. The aim of this study was to explore the effects of NAM on hydrogen peroxide (H
2 O2 )-induced oxidative injury in bovine intestinal epithelial cells (BIECs) and its potential mechanism. The results showed that NAM increased the cell viability and total antioxidant capacity (T-AOC) and decreased the release of lactate dehydrogenase (LDH) in BIECs challenged by H2 O2 . The NAM exhibited increased expression of catalase, superoxide dismutase 2, and tight junction proteins. The expression of autophagy-related proteins was increased in BIECs challenged by H2 O2 , and NAM significantly decreased the expression of autophagy-related proteins. When an autophagy-specific inhibitor was used, the oxidative injury in BIECs was not alleviated by NAM, and the T-AOC and the release of LDH were not affected. Collectively, these results indicated that NAM could alleviate oxidative injury in BIECs by enhancing antioxidant capacity and increasing the expression of tight junction proteins, and autophagy played a crucial role in the alleviation. [ABSTRACT FROM AUTHOR]- Published
- 2024
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23. Evaluation of mucosal-associated invariant T-cells as a potential biomarker to predict infection risk in liver cirrhosis.
- Author
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Bengtsson, Bonnie, Maucourant, Christopher, Sandberg, Johan K., Björkström, Niklas K., and Hagström, Hannes
- Subjects
CIRRHOSIS of the liver ,BIOMARKERS ,BACTERIAL diseases ,INFECTION ,FLOW cytometry ,ASCITIC fluids ,T cells - Abstract
Background and aims: Infection is a serious complication in patients with cirrhosis. Mucosal-associated invariant T (MAIT) cells are involved in the immune defense against infections and known to be impaired in several chronic conditions, including cirrhosis. Here, we evaluated if MAIT cell levels in peripheral blood are associated with risk of bacterial infections in patients with cirrhosis. Methods: Patients with cirrhosis seen at the Karolinska University Hospital, Stockholm, Sweden, between 2016 and 2019 were included. Levels of MAIT cells in peripheral blood were determined using flow cytometry. Baseline and follow-up data after at least two years of follow-up were collected by chart review for the primary outcome (bacterial infection) and secondary outcomes (decompensation and death). Competing risk and Cox regression were performed. Results: We included 106 patients with cirrhosis. The median MAIT cells fraction in the circulation was 0.8% in cirrhosis compared to 6.1% in healthy controls. In contrast to our hypothesis, we found an association in the adjusted analysis between relatively preserved MAIT cell levels, and a slightly higher risk to develop bacterial infections (adjusted subdistribution hazard ratio (aSHR) 1.15 (95%CI = 1.01–1.31). However, MAIT cell levels were not associated with the risk of hepatic decompensation (aSHR 1.19 (95%CI = 0.91–1.56)) nor with death (adjusted hazard ratio 1.10 (95%CI = 0.97–1.22)). Conclusions: Relatively preserved MAIT cell levels in blood of patients with cirrhosis were associated with a somewhat higher risk of bacterial infections. The clinical relevance of this might not be strong. MAIT cells might however be an interesting biomarker to explore in future studies. [ABSTRACT FROM AUTHOR]
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- 2024
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24. Does Autophagy have a Role in the Pathogenesis of Pediatric Hepatic Steatosis?
- Author
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Gadallah, Marwa Salah, Kandil, Mona, Holah, Nanis Shawky, Sobhy, Gihan Ahmed, Ahmed, Mohamed Mohamady, El-Gammal, Shaymaa Sabry, and Ehsan, Nermine Ahmed
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- 2024
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25. Vaccine Responses in Patients with Liver Cirrhosis: From the Immune System to the Gut Microbiota.
- Author
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Airola, Carlo, Andaloro, Silvia, Gasbarrini, Antonio, and Ponziani, Francesca Romana
- Subjects
VACCINE effectiveness ,IMMUNE system ,COVID-19 ,GUT microbiome ,HEPATITIS B virus - Abstract
Vaccines prevent a significant number of deaths annually. However, certain populations do not respond adequately to vaccination due to impaired immune systems. Cirrhosis, a condition marked by a profound disruption of immunity, impairs the normal immunization process. Critical vaccines for cirrhotic patients, such as the hepatitis A virus (HAV), hepatitis B virus (HBV), influenza, pneumococcal, and coronavirus disease 19 (COVID-19), often elicit suboptimal responses in these individuals. The humoral response, essential for immunization, is less effective in cirrhosis due to a decline in B memory cells and an increase in plasma blasts, which interfere with the creation of a long-lasting response to antigen vaccination. Additionally, some T cell subtypes exhibit reduced activation in cirrhosis. Nonetheless, the persistence of memory T cell activity, while not preventing infections, may help to attenuate the severity of diseases in these patients. Alongside that, the impairment of innate immunity, particularly in dendritic cells (DCs), prevents the normal priming of adaptive immunity, interrupting the immunization process at its onset. Furthermore, cirrhosis disrupts the gut–liver axis balance, causing dysbiosis, reduced production of short-chain fatty acids (SCFAs), increased intestinal permeability, and bacterial translocation. Undermining the physiological activity of the immune system, these alterations could impact the vaccine response. Enhancing the understanding of the molecular and cellular factors contributing to impaired vaccination responses in cirrhotic patients is crucial for improving vaccine efficacy in this population and developing better prevention strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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26. Phenotype and function of MAIT cells in patients with alveolar echinococcosis.
- Author
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Jintian Li, Hanyue Zhao, Guodong Lv, Aimulajiang, Kalibixiati, Liang Li, Renyong Lin, and Aji, Tuerganaili
- Subjects
CELL physiology ,ECHINOCOCCOSIS ,ECHINOCOCCUS multilocularis ,HEPATIC fibrosis ,CHEMOKINE receptors ,HEPATIC echinococcosis - Abstract
Mucosal-associated invariant T (MAIT) cells are a subpopulation of unconventional T cells widely involved in chronic liver diseases. However, the potential role and regulating factors of MAIT cells in alveolar echinococcosis (AE), a zoonotic parasitic disease by Echinococcus multilocularis (E. multilocularis) larvae chronically parasitizing liver organs, has not yet been studied. Blood samples (n=29) and liver specimens (n=10) from AE patients were enrolled. The frequency, phenotype, and function of MAIT cells in peripheral blood and liver tissues of AE patients were detected by flow cytometry. The morphology and fibrosis of liver tissue were examined by histopathology and immunohistochemistry. The correlation between peripheral MAIT cell frequency and serologic markers was assessed by collecting clinicopathologic characteristics of AE patients. And the effect of in vitro stimulation with E. multilocularis antigen (Emp) on MAIT cells. In this study, MAIT cells are decreased in peripheral blood and increased in the close-to-lesion liver tissues, especially in areas of fibrosis. Circulating MAIT exhibited activation and exhaustion phenotypes, and intrahepatic MAIT cells showed increased activation phenotypes with increased IFN-γ and IL-17A, and high expression of CXCR5 chemokine receptor. Furthermore, the frequency of circulating MAIT cells was correlated with the size of the lesions and liver function in patients with AE. After excision of the lesion site, circulating MAIT cells returned to normal levels, and the serum cytokines IL-8, IL-12, and IL-18, associated with MAIT cell activation and apoptosis, were altered. Our results demonstrate the status of MAIT cell distribution, functional phenotype, and migration in peripheral blood and tissues of AE patients, highlighting their potential as biomarkers and therapeutic targets. [ABSTRACT FROM AUTHOR]
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- 2024
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27. Molecular Imaging of Fibrosis in Benign Diseases: An Overview of the State of the Art.
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Zhang, Yongbai, Huang, Wenpeng, Jiao, Hao, Song, Lele, and Kang, Lei
- Subjects
FIBROSIS ,DISEASE progression ,MOLECULAR probes ,FOOTBALL techniques ,MOLECULAR spectroscopy - Abstract
Fibrosis is a progressive pathological process participating in the progression of many diseases and can ultimately result in organ malfunction and failure. Around 45% of deaths in the United States are believed to be attributable to fibrotic disorders, and there are no favorable treatment regiments available to meet the need of blocking fibrogenesis, reversing established fibrosis, and curing diseases, especially in the terminal stage. Therefore, early detection and continuous monitoring provide valuable benefits for patients. Among all the advanced techniques developed in recent years for fibrosis evaluation, molecular imaging stands out with its distinct advantage of visualizing biochemical processes and patterns of target localization at the molecular and cellular level. In this review, we summarize the current state of the art in molecular imaging of benign fibrosis diseases. We will first introduce molecular pathways underlying fibrosis processes and potential targets. We will then elaborate on molecular probes that have been developed thus far, expounding on their mechanisms and current states of translational advancement. Finally, we will delineate the extant challenges impeding further progress in this area and the prospective benefits after overcoming these problems. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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28. Mucosal-associated invariant T cells in infectious diseases of respiratory system: recent advancements and applications.
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Lin, Xue, Wang, Ye, and He, Yanqi
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RESPIRATORY diseases ,RESPIRATORY organs ,T cells ,MAJOR histocompatibility complex ,HOMEOSTASIS ,VITAMIN B complex ,T cell receptors ,LYMPHOCYTE subsets - Abstract
Mucosal-associated invariant T (MAIT) cells are an atypical subset of T lymphocytes, which have a highly conserved semi-constant αβ chain of T-cell receptor (TCR) and recognize microbe-derived vitamin B metabolites via major histocompatibility complex class I related-1 molecule (MR1). MAIT cells get activated mainly through unique TCR-dependent and TCR-independent pathways, and express multiple functional and phenotypic traits, including innate-like functionality, T helper (Th) 1 cell immunity, Th 17 cell immunity, and tissue homing. Given the functions, MAIT cells are extensively reported to play a key role in mucosal homeostasis and infectious diseases. In the current work, we review the basic characteristics of MAIT cells and their roles in mucosal homeostasis and development of respiratory infectious diseases as well as their potential therapeutic targets. [ABSTRACT FROM AUTHOR]
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- 2024
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29. Developing a straightforward route toward the synthesis of arylaminomaleimides by palladium-catalyzed arylation of one-pot synthesized aminomaleimides.
- Author
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Taheri, Elmira and Jafarpour, Farnaz
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- 2024
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30. Empowering Support for Family Members of Patients With Traumatic Brain Injury During the Acute Care: Insights From Family Members and Nurses.
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Lindlöf J, Turunen H, Coco K, Huhtakangas J, Verhaeghe S, and Välimäki T
- Abstract
Aim: To investigate the perceptions of family members (FMs) of patients with traumatic brain injury (TBI) and nurses on empowering support and its implementation during the acute phase within Finnish neurosurgical and neurological care in hospital settings, focusing on identifying similarities and differences in their viewpoints., Design: Participatory qualitative descriptive study., Methods: Data were collected from seven FMs and 11 nurses using the World Café method in November 2019. An abductive approach was employed for data analysis, combining deductive interpretation within the conceptual framework of empowering support and inductive content analysis., Results: Four main themes were identified: (1) FMs' diverse information and guidance needs of TBI, treatment and its impact on family life, (2) support based on empowering FMs in participation, competence and decision-making, (3) empowering FMs through collaborative nursing practices and interprofessional support, and (4) internal and external hospital support enhancing and promoting the empowerment of FMs., Conclusion: The perceptions of FMs and nurses regarding empowering support were largely consistent, yet diverged in its implementation in nursing practice. Nurses play a crucial role in fostering the empowerment of FMs; however, further research is needed to explore the impact of organisational and community factors on the implementation of empowering support., Impact: Our study contributes to advancing nursing practices by underscoring the necessity for a paradigm shift towards a family-centred approach. Furthermore, it emphasises the urgency for standardising nursing practices to ensure equitable access to empowering support for FMs, applicable across various care settings for patients with TBI., Public Contribution: This review is part of a larger research project in which FMs of patients with TBI and nurses were involved in designing the project., Reporting Method: This study was reported using the Consolidated Criteria for Reporting Qualitative Checklist for qualitative studies., (© 2024 The Author(s). Journal of Advanced Nursing published by John Wiley & Sons Ltd.)
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- 2024
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31. Natural Killer T Cell Diversity and Immunotherapy.
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Tognarelli, Eduardo I., Gutiérrez-Vera, Cristián, Palacios, Pablo A., Pasten-Ferrada, Ignacio A., Aguirre-Muñoz, Fernanda, Cornejo, Daniel A., González, Pablo A., and Carreño, Leandro J.
- Subjects
COMMUNICABLE diseases ,INFLAMMATION ,KILLER cells ,IMMUNOTHERAPY - Abstract
Simple Summary: Cell-based oncotherapies are gaining considerable terrain in the last years thanks to the development of safe and promising treatments against cancer. A better understanding of the roles and functional capacities of cellular immune components displaying antitumor activity will likely potentiate these encouraging results. In this review, we summarize the properties and uses of a particular type of immune cells that recognize lipid-derived determinants presented on the cell surface. Such immune cells, known as invariant Natural Killer T (iNKT) cells have the potential to significantly favor anti-tumoral adaptive immune responses. Currently, they are being extensively evaluated in preclinical settings and may soon reach clinical trials to ameliorate anti-cancer treatments. Invariant natural killer T cells (iNKTs), a type of unconventional T cells, share features with NK cells and have an invariant T cell receptor (TCR), which recognizes lipid antigens loaded on CD1d molecules, a major histocompatibility complex class I (MHC-I)-like protein. This interaction produces the secretion of a wide array of cytokines by these cells, including interferon gamma (IFN-γ) and interleukin 4 (IL-4), allowing iNKTs to link innate with adaptive responses. Interestingly, molecules that bind CD1d have been identified that enable the modulation of these cells, highlighting their potential pro-inflammatory and immunosuppressive capacities, as required in different clinical settings. In this review, we summarize key features of iNKTs and current understandings of modulatory α-galactosylceramide (α-GalCer) variants, a model iNKT cell activator that can shift the outcome of adaptive immune responses. Furthermore, we discuss advances in the development of strategies that modulate these cells to target pathologies that are considerable healthcare burdens. Finally, we recapitulate findings supporting a role for iNKTs in infectious diseases and tumor immunotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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32. Effect of Docosahexaenoic Acid Encapsulation with Whey Proteins on Rat Growth and Tissue Endocannabinoid Profile.
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Wang, Jun, Ossemond, Jordane, Le Gouar, Yann, Boissel, Françoise, Dupont, Didier, and Pédrono, Frédérique
- Abstract
Modifying the food structure allows a nutrient to be delivered differently, which can modify not only its digestion process but also its subsequent metabolism. In this study, rats received 3 g of omelette daily containing docosahexaenoic acid (DHA) as crude oil or previously encapsulated with whey proteins, whereas a control group received a DHA-free omelette. The results showed that DHA encapsulation markedly induced a different feeding behaviour so animals ate more and grew faster. Then, after four weeks, endocannabinoids and other N-acyl ethanolamides were quantified in plasma, brain, and heart. DHA supplementation strongly reduced endocannabinoid derivatives from omega-6 fatty acids. However, DHA encapsulation had no particular effect, other than a great increase in the content of DHA-derived docosahexaenoyl ethanolamide in the heart. While DHA supplementation has indeed shown an effect on cannabinoid profiles, its physiological effect appears to be mediated more through more efficient digestion of DHA oil droplets in the case of DHA encapsulation. Thus, the greater release of DHA and other dietary cannabinoids present may have activated the cannabinoid system differently, possibly more locally along the gastrointestinal tract. However, further studies are needed to evaluate the synergy between DHA encapsulation, fasting, hormones regulating food intake, and animal growth. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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33. Dysfunctions of Circulating Adaptive Immune Cells in End-Stage Liver Disease.
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Liu, Tong, Chouik, Yasmina, Lebossé, Fanny, and Khamri, Wafa
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INFECTION risk factors ,B cells ,INFLAMMATION ,IMMUNOSUPPRESSION ,CIRRHOSIS of the liver ,CELL physiology ,BLOOD circulation ,T cells ,LIVER failure ,PHENOTYPES - Abstract
End-stage liver disease (ESLD) from acute liver failure to compensated advanced chronic liver disease and decompensated cirrhosis at different stages (chronic decompensation, acute decompensation with or without acute-on-chronic liver failure) has high disease severity and poor patient outcome. Infection is a common complication in patients with ESLD and it is associated with a high mortality rate. Multiple mechanisms are involved in this marked susceptibility to infections, noticeably the inadequate immune response known as immune paresis, as part of cirrhosis-associated immune dysfunction (CAID). Specifically in the adaptive immune arm, lymphocyte impairments—including inadequate activation, reduced ability to secrete effector molecules and enhanced immune suppressive phenotypes—result in compromised systemic immune responses and increased risk of infections. This review summarises current knowledge of alterations in adaptive immune responsiveness and their underlying mechanisms in ESLD. Understanding these mechanisms is of crucial importance in the identification of potential therapeutic targets and applications of targeted treatments beyond antimicrobials, such as immunotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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34. Cu‐Catalyzed C(sp2)−H Bond Aminosulfonylation of Maleimides.
- Author
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Cen, Kaili, Zeng, Zhouting, Shen, Wangzhen, Feng, Yuting, Yan, Pingzhi, Wei, Jiahao, Hou, Qian, Fu, Junjie, Xia, Xiaotong, and Cai, Jinhui
- Subjects
MALEIMIDES ,RADICALS (Chemistry) ,ATOMOXETINE ,FLUOXETINE ,DRUGS ,COPPER - Abstract
A copper‐catalyzed direct C(sp2)−H bond aminosulfonylation of maleimides is demonstrated. This protocol enables concurrent construction of C(sp2)−N and C(sp2)−SO2 bonds in one‐step and features high efficiency, broad substrate scopes, good functional‐group tolerance, and mild reaction conditions. Preliminary mechanistic studies indicate that the reaction probably involves a radical way. Significantly, this method is applicable to synthesize derivatives of pharmaceuticals such as Desloratadine, Fluoxetine, Atomoxetine, and Maprotiline. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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35. Role of TRP Channels in Liver-Related Diseases.
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Liu, Yusheng, Lyu, Yihan, Zhu, Lijuan, and Wang, Hongmei
- Subjects
TRP channels ,HEPATIC fibrosis ,VASCULAR endothelial cells ,LIVER cells ,KUPFFER cells ,THERAPEUTICS - Abstract
The liver plays a crucial role in preserving the homeostasis of an entire organism by metabolizing both endogenous and exogenous substances, a process that relies on the harmonious interactions of hepatocytes, hepatic stellate cells (HSCs), Kupffer cells (KCs), and vascular endothelial cells (ECs). The disruption of the liver's normal structure and function by diverse pathogenic factors imposes a significant healthcare burden. At present, most of the treatments for liver disease are palliative in nature, rather than curative or restorative. Transient receptor potential (TRP) channels, which are extensively expressed in the liver, play a crucial role in regulating intracellular cation concentration and serve as the origin or intermediary stage of certain signaling pathways that contribute to liver diseases. This review provides an overview of recent developments in liver disease research, as well as an examination of the expression and function of TRP channels in various liver cell types. Furthermore, we elucidate the molecular mechanism by which TRP channels mediate liver injury, liver fibrosis, and hepatocellular carcinoma (HCC). Ultimately, the present discourse delves into the current state of research and extant issues pertaining to the targeting of TRP channels in the treatment of liver diseases and other ailments. Despite the numerous obstacles encountered, TRP channels persist as an extremely important target for forthcoming clinical interventions aimed at treating liver diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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36. Impact of Lipid Metabolism on Macrophage Polarization: Implications for Inflammation and Tumor Immunity.
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Vassiliou, Evros and Farias-Pereira, Renalison
- Subjects
LIPID metabolism ,PHAGOCYTOSIS ,FOAM cells ,MACROPHAGES ,IMMUNITY ,INFLAMMATORY mediators ,LIPIDS - Abstract
Macrophage polarization is influenced by lipids, which also exert significant control over macrophage functions. Lipids and their metabolites are players in intricate signaling pathways that modulate macrophages' responses to pathogens, phagocytosis, ferroptosis, and inflammation. This review focuses on lipid metabolism and macrophage functions and addresses potential molecular targets for the treatment of macrophage-related diseases. While lipogenesis is crucial for lipid accumulation and phagocytosis in M1 macrophages, M2 macrophages likely rely on fatty acid β-oxidation to utilize fatty acids as their primary energy source. Cholesterol metabolism, regulated by factors such as SREBPs, PPARs, and LXRs, is associated with the cholesterol efflux capacity and the formation of foam cells (M2-like macrophages). Foam cells, which are targets for atherosclerosis, are associated with an increase in inflammatory cytokines. Lipolysis and fatty acid uptake markers, such as CD36, also contribute to the production of cytokines. Enhancing the immune system through the inhibition of lipid-metabolism-related factors can potentially serve as a targeted approach against tumor cells. Cyclooxygenase inhibitors, which block the conversion of arachidonic acid into various inflammatory mediators, influence macrophage polarization and have generated attention in cancer research. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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37. Programmed cell death and lipid metabolism of macrophages in NAFLD.
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Zhun Xiao, Minghao Liu, Fangming Yang, Guangwei Liu, Jiangkai Liu, Wenxia Zhao, Suping Ma, and Zhongping Duan
- Subjects
APOPTOSIS ,LIPID metabolism ,NON-alcoholic fatty liver disease ,HEPATITIS ,MACROPHAGES - Abstract
Non-alcoholic fatty liver disease (NAFLD) has now become the leading chronic liver disease worldwide with lifestyle changes. This may lead to NAFLD becoming the leading cause of end-stage liver disease in the future. To date, there are still no effective therapeutic drugs for NAFLD. An in-depth exploration of the pathogenesis of NAFLD can help to provide a basis for new therapeutic agents or strategies. As the most important immune cells of the liver, macrophages play an important role in the occurrence and development of liver inflammation and are expected to become effective targets for NAFLD treatment. Programmed cell death (PCD) of macrophages plays a regulatory role in phenotypic transformation, and there is also a certain connection between different types of PCD. However, how PCD regulates macrophage polarization has still not been systematically elucidated. Based on the role of lipid metabolic reprogramming in macrophage polarization, PCD may alter the phenotype by regulating lipid metabolism. We reviewed the effects of macrophages on inflammation in NAFLD and changes in their lipid metabolism, as well as the relationship between different types of PCD and lipid metabolism in macrophages. Furthermore, interactions between different types of PCD and potential therapeutic agents targeting of macrophages PCD are also explored. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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38. Classic costimulatory interactions in MAIT cell responses: from gene expression to immune regulation.
- Author
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Wang, Nicole I, Ninkov, Marina, and Haeryfar, S M Mansour
- Subjects
GENETIC regulation ,KILLER cells ,T helper cells ,TUMOR necrosis factors ,B cells ,LYMPHATIC metastasis ,AUTOIMMUNE diseases - Abstract
Summary: Mucosa-associated invariant T (MAIT) cells are evolutionarily conserved, innate-like T lymphocytes with enormous immunomodulatory potentials. Due to their strategic localization, their invariant T cell receptor (iTCR) specificity for major histocompatibility complex-related protein 1 (MR1) ligands of commensal and pathogenic bacterial origin, and their sensitivity to infection-elicited cytokines, MAIT cells are best known for their antimicrobial characteristics. However, they are thought to also play important parts in the contexts of cancer, autoimmunity, vaccine-induced immunity, and tissue repair. While cognate MR1 ligands and cytokine cues govern MAIT cell maturation, polarization, and peripheral activation, other signal transduction pathways, including those mediated by costimulatory interactions, regulate MAIT cell responses. Activated MAIT cells exhibit cytolytic activities and secrete potent inflammatory cytokines of their own, thus transregulating the biological behaviors of several other cell types, including dendritic cells, macrophages, natural killer cells, conventional T cells, and B cells, with significant implications in health and disease. Therefore, an in-depth understanding of how costimulatory pathways control MAIT cell responses may introduce new targets for optimized MR1/MAIT cell-based interventions. Herein, we compare and contrast MAIT cells and mainstream T cells for their expression of classic costimulatory molecules belonging to the immunoglobulin superfamily and the tumor necrosis factor (TNF)/TNF receptor superfamily, based not only on the available literature but also on our transcriptomic analyses. We discuss how these molecules participate in MAIT cells' development and activities. Finally, we introduce several pressing questions vis-à-vis MAIT cell costimulation and offer new directions for future research in this area. The CD40L-CD40 costimulatory axis modulates mucosa-associated invariant T (MAIT) cells' functions and mediates their bidirectional crosstalk with other important immunocytes. Activated MAIT cells rely on this pathway to promote dendritic cell maturation, resulting in type-I cytokine production as well as follicular helper T cell expansion in lymph nodes. In addition, CD40L signaling in MAIT cells enhances B cell activation and antibody responses. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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39. Paeoniflorin Coordinates Macrophage Polarization and Mitigates Liver Inflammation and Fibrogenesis by Targeting the NF-κB/HIF-1α Pathway in CCl4-Induced Liver Fibrosis.
- Author
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Liu, Yang, He, Chun-Yu, Yang, Xue-Mei, Chen, Wei-Cong, Zhang, Ming-Jia, Zhong, Xiao-Dan, Chen, Wei-Guang, Zhong, Bing-Lian, He, Song-Qi, and Sun, Hai-Tao
- Subjects
INFLAMMATION prevention ,IN vitro studies ,PROTEINS ,ALBUMINS ,REVERSE transcriptase polymerase chain reaction ,STATISTICS ,MEDICINAL plants ,IN vivo studies ,ANIMAL experimentation ,WESTERN immunoblotting ,ONE-way analysis of variance ,CIRRHOSIS of the liver ,MACROPHAGES ,NF-kappa B ,CELLULAR signal transduction ,GENE expression ,RATS ,CELL survival ,HYALURONIC acid ,FLUORESCENT antibody technique ,DESCRIPTIVE statistics ,RESEARCH funding ,PLANT extracts ,MOLECULAR structure ,LIVER cells ,DATA analysis software ,DATA analysis ,ASPARTATE aminotransferase ,ALANINE aminotransferase ,BILIRUBIN - Abstract
Liver fibrosis is a disease largely driven by resident and recruited macrophages. The phenotypic switch of hepatic macrophages can be achieved by chemo-attractants and cytokines. During a screening of plants traditionally used to treat liver diseases in China, paeoniflorin was identified as a potential drug that affects the polarization of macrophages. The aim of this study was to evaluate the therapeutic effects of paeoniflorin in an animal model of liver fibrosis and explore its underlying mechanisms. Liver fibrosis was induced in Wistar rats via an intraperitoneal injection of CCl
4 . In addition, the RAW264.7 macrophages were cultured in the presence of CoCl2 to simulate a hypoxic microenvironment of fibrotic livers in vitro. The modeled rats were treated daily with either paeoniflorin (100, 150, and 200 mg/kg) or YC-1 (2 mg/kg) for 8 weeks. Hepatic function, inflammation and fibrosis, activation of hepatic stellate cells (HSC), and extracellular matrix (ECM) deposition were assessed in the in vivo and in vitro models. The expression levels of M1 and M2 macrophage markers and the NF- κ B/HIF-1 α pathway factors were measured using standard assays. Paeoniflorin significantly alleviated hepatic inflammation and fibrosis, as well as hepatocyte necrosis in the CCl4 -induced fibrosis model. Furthermore, paeoniflorin also inhibited HSC activation and reduced ECM deposition both in vivo and in vitro. Mechanistically, paeoniflorin restrained M1 macrophage polarization and induced M2 polarization in the fibrotic liver tissues as well as in the RAW264.7 cells grown under hypoxic conditions by inactivating the NF- κ B/HIF-1 α signaling pathway. In conclusion, paeoniflorin exerts its anti-inflammatory and anti-fibrotic effects in the liver by coordinating macrophage polarization through the NF- κ B/HIF-1 α pathway. [ABSTRACT FROM AUTHOR]- Published
- 2023
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40. Autophagy and mitophagy: physiological implications in kidney inflammation and diseases.
- Author
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Bhatia, Divya and Choi, Mary E.
- Subjects
AUTOPHAGY ,APOPTOSIS ,CHRONIC kidney failure ,CELL survival ,ACUTE kidney failure - Abstract
Autophagy is a ubiquitous intracellular cytoprotective quality control program that maintains cellular homeostasis by recycling superfluous cytoplasmic components (lipid droplets, protein, or glycogen aggregates) and invading pathogens. Mitophagy is a selective form of autophagy that by recycling damaged mitochondrial material, which can extracellularly act as damageassociated molecular patterns, prevents their release. Autophagy and mitophagy are indispensable for the maintenance of kidney homeostasis and exert crucial functions during both physiological and disease conditions. Impaired autophagy and mitophagy can negatively impact the pathophysiological state and promote its progression. Autophagy helps in maintaining structural integrity of the kidney. Mitophagy-mediated mitochondrial quality control is explicitly critical for regulating cellular homeostasis in the kidney. Both autophagy and mitophagy attenuate inflammatory responses in the kidney. An accumulating body of evidence highlights that persistent kidney injury-induced oxidative stress can contribute to dysregulated autophagic and mitophagic responses and cell death. Autophagy and mitophagy also communicate with programmed cell death pathways (apoptosis and necroptosis) and play important roles in cell survival by preventing nutrient deprivation and regulating oxidative stress. Autophagy and mitophagy are activated in the kidney after acute injury. However, their aberrant hyperactivation can be deleterious and cause tissue damage. The findings on the functions of autophagy and mitophagy in various models of chronic kidney disease are heterogeneous and cell type- and context-specific dependent. In this review, we discuss the roles of autophagy and mitophagy in the kidney in regulating inflammatory responses and during various pathological manifestations. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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41. The "Domino effect" in MASLD: The inflammatory cascade of steatohepatitis.
- Author
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Mladenić K, Lenartić M, Marinović S, Polić B, and Wensveen FM
- Subjects
- Adult, Humans, B-Lymphocytes, Inflammation, Tumor Microenvironment, Metabolic Diseases, Fatty Liver, Liver Neoplasms
- Abstract
Metabolic dysfunction-associated steatotic liver disease (MASLD) is an increasingly common complication of obesity, affecting over a quarter of the global adult population. A key event in the pathophysiology of MASLD is the development of metabolic-associated steatohepatitis (MASH), which greatly increases the chances of developing cirrhosis and hepatocellular carcinoma. The underlying cause of MASH is multifactorial, but accumulating evidence indicates that the inflammatory process in the hepatic microenvironment typically follows a pattern that can be roughly divided into three stages: (1) Detection of hepatocyte stress by tissue-resident immune cells including γδ T cells and CD4
- CD8- double-negative T cells, followed by their secretion of pro-inflammatory mediators, most notably IL-17A. (2) Recruitment of pro-inflammatory cells, mostly of the myeloid lineage, and initiation of inflammation through secretion of effector-type cytokines such as TNF, TGF-β, and IL-1β. (3) Escalation of the inflammatory response by recruitment of lymphocytes including Th17, CD8 T, and B cells leading to chronic inflammation, hepatic stellate cell activation, and fibrosis. Here we will discuss these three stages and how they are consecutively linked like falling domino tiles to the pathophysiology of MASH. Moreover, we will highlight the clinical potential of inflammation as a biomarker and therapeutic target for the treatment of MASLD., (© 2024 Wiley‐VCH GmbH.)- Published
- 2024
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42. Regulatory mechanism of FCGR2A in macrophage polarization and its effects on intervertebral disc degeneration.
- Author
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Luo J, Jin G, Cui S, Wang H, and Liu Q
- Subjects
- Animals, Gene Expression Profiling, Macrophages, NF-kappa B genetics, NF-kappa B metabolism, Nucleus Pulposus metabolism, Humans, Rats, Intervertebral Disc Degeneration genetics, Intervertebral Disc Degeneration metabolism, Receptors, IgG metabolism
- Abstract
Intervertebral disc degeneration (IDD) poses a significant health burden, necessitating a deeper understanding of its molecular underpinnings. Transcriptomic analysis reveals 485 differentially expressed genes (DEGs) associated with IDD, underscoring the importance of immune regulation. Weighted gene co-expression network analysis (WGCNA) identifies a yellow module strongly correlated with IDD, intersecting with 197 DEGs. Protein-protein interaction (PPI) analysis identifies ITGAX, MMP9 and FCGR2A as hub genes, predominantly expressed in macrophages. Functional validation through in vitro and in vivo experiments demonstrates the pivotal role of FCGR2A in macrophage polarization and IDD progression. Mechanistically, FCGR2A knockdown suppresses M1 macrophage polarization and NF-κB phosphorylation while enhancing M2 polarization and STAT3 activation, leading to ameliorated IDD in animal models. This study sheds light on the regulatory function of FCGR2A in macrophage polarization, offering novel insights for IDD intervention strategies. KEY POINTS: This study unveils the role of FCGR2A in intervertebral disc (IVD) degeneration (IDD). FCGR2A knockdown mitigates IDD in cellular and animal models. Single-cell RNA-sequencing uncovers diverse macrophage subpopulations in degenerated IVDs. This study reveals the molecular mechanism of FCGR2A in regulating macrophage polarization. This study confirms the role of the NF-κB/STAT3 pathway in regulating macrophage polarization in IDD., (© 2024 The Authors. The Journal of Physiology © 2024 The Physiological Society.)
- Published
- 2024
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43. Glycation-driven matrix crosslinking in cirrhosis.
- Author
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Kollet, Orit and Sagi, Irit
- Published
- 2023
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44. Fluorinated Benzofuran and Dihydrobenzofuran as Anti-Inflammatory and Potential Anticancer Agents.
- Author
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Ayoub, Abeer J., El-Achkar, Ghewa A., Ghayad, Sandra E., Hariss, Layal, Haidar, Razan H., Antar, Leen M., Mallah, Zahraa I., Badran, Bassam, Grée, René, Hachem, Ali, Hamade, Eva, and Habib, Aida
- Subjects
BENZOFURAN ,INFLAMMATORY mediators ,ANTINEOPLASTIC agents ,NITRIC-oxide synthases ,ESTERS ,PHARMACEUTICAL chemistry ,BENZOFURANS - Abstract
Benzofuran and 2,3-dihydrobenzofuran scaffolds are heterocycles of high value in medicinal chemistry and drug synthesis. Targeting inflammation in cancer associated with chronic inflammation is a promising therapy. In the present study, we investigated the anti-inflammatory effects of fluorinated benzofuran and dihydrobenzofuran derivatives in macrophages and in the air pouch model of inflammation, as well as their anticancer effects in the human colorectal adenocarcinoma cell line HCT116. Six of the nine compounds suppressed lipopolysaccharide-stimulated inflammation by inhibiting the expression of cyclooxygenase-2 and nitric oxide synthase 2 and decreased the secretion of the tested inflammatory mediators. Their IC
50 values ranged from 1.2 to 9.04 µM for interleukin-6; from 1.5 to 19.3 µM for Chemokine (C-C) Ligand 2; from 2.4 to 5.2 µM for nitric oxide; and from 1.1 to 20.5 µM for prostaglandin E2 . Three novel synthesized benzofuran compounds significantly inhibited cyclooxygenase activity. Most of these compounds showed anti-inflammatory effects in the zymosan-induced air pouch model. Because inflammation may lead to tumorigenesis, we tested the effects of these compounds on the proliferation and apoptosis of HCT116. Two compounds with difluorine, bromine, and ester or carboxylic acid groups inhibited the proliferation by approximately 70%. Inhibition of the expression of the antiapoptotic protein Bcl-2 and concentration-dependent cleavage of PARP-1, as well as DNA fragmentation by approximately 80%, were described. Analysis of the structure–activity relationship suggested that the biological effects of benzofuran derivatives are enhanced in the presence of fluorine, bromine, hydroxyl, and/or carboxyl groups. In conclusion, the designed fluorinated benzofuran and dihydrobenzofuran derivatives are efficient anti-inflammatory agents, with a promising anticancer effect and a combinatory treatment in inflammation and tumorigenesis in cancer microenvironments. [ABSTRACT FROM AUTHOR]- Published
- 2023
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45. Autophagy prevents early proinflammatory responses and neutrophil recruitment during Mycobacterium tuberculosis infection without affecting pathogen burden in macrophages.
- Author
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Kinsella, Rachel L., Kimmey, Jacqueline M., Smirnov, Asya, Woodson, Reilly, Gaggioli, Margaret R., Chavez, Sthefany M., Kreamalmeyer, Darren, and Stallings, Christina L.
- Subjects
MYCOBACTERIUM tuberculosis ,MYCOBACTERIAL diseases ,NEUTROPHILS ,AUTOPHAGY ,MACROPHAGES ,CELL culture ,CHEMOKINE receptors - Abstract
The immune response to Mycobacterium tuberculosis infection determines tuberculosis disease outcomes, yet we have an incomplete understanding of what immune factors contribute to a protective immune response. Neutrophilic inflammation has been associated with poor disease prognosis in humans and in animal models during M. tuberculosis infection and, therefore, must be tightly regulated. ATG5 is an essential autophagy protein that is required in innate immune cells to control neutrophil-dominated inflammation and promote survival during M. tuberculosis infection; however, the mechanistic basis for how ATG5 regulates neutrophil recruitment is unknown. To interrogate what innate immune cells require ATG5 to control neutrophil recruitment during M. tuberculosis infection, we used different mouse strains that conditionally delete Atg5 in specific cell types. We found that ATG5 is required in CD11c
+ cells (lung macrophages and dendritic cells) to control the production of proinflammatory cytokines and chemokines during M. tuberculosis infection, which would otherwise promote neutrophil recruitment. This role for ATG5 is autophagy dependent, but independent of mitophagy, LC3-associated phagocytosis, and inflammasome activation, which are the most well-characterized ways that autophagy proteins regulate inflammation. In addition to the increased proinflammatory cytokine production from macrophages during M. tuberculosis infection, loss of ATG5 in innate immune cells also results in an early induction of TH 17 responses. Despite prior published in vitro cell culture experiments supporting a role for autophagy in controlling M. tuberculosis replication in macrophages, the effects of autophagy on inflammatory responses occur without changes in M. tuberculosis burden in macrophages. These findings reveal new roles for autophagy proteins in lung resident macrophages and dendritic cells that are required to suppress inflammatory responses that are associated with poor control of M. tuberculosis infection. The immune response to Mycobacterium tuberculosis infection determines tuberculosis disease outcomes. This study identifies new roles for the autophagy pathway in regulating proinflammatory responses of lung macrophages to M. tuberculosis infection, without controlling pathogen replication. [ABSTRACT FROM AUTHOR]- Published
- 2023
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- View/download PDF
46. Major roles of kupffer cells and macrophages in NAFLD development.
- Author
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Soo-Jeung Park, Diaz, Josefina Garcia, Um, Eugene, and Hahn, Young S.
- Subjects
KUPFFER cells ,NON-alcoholic fatty liver disease ,MACROPHAGES - Abstract
Non-alcoholic fatty liver disease (NAFLD) is an important public health problem with growing numbers of NAFLD patients worldwide. Pathological conditions are different in each stage of NAFLD due to various factors. Preclinical and clinical studies provide evidence for a crucial role of immune cells in NAFLD progression. Liver-resident macrophages, kupffer cells (KCs), and monocytes-derived macrophages are the key cell types involved in the progression of NAFLD, non-alcoholic steatohepatitis (NASH), and hepatocellular carcinoma (HCC). Their unique polarization contributes to the progression of NAFLD. KCs are phagocytes with self-renewal abilities and play a role in regulating and maintaining homeostasis. Upon liver damage, KCs are activated and colonized at the site of the damaged tissue. The secretion of inflammatory cytokines and chemokines by KCs play a pivotal role in initiating NAFLD pathogenesis. This review briefly describes the role of immune cells in the immune system in NAFLD, and focuses on the pathological role and molecular pathways of KCs and recruited macrophages. In addition, the relationship between macrophages and insulin resistance is described. Finally, the latest therapeutics that target KCs and macrophages are summarized for the prevention and treatment of NAFLD. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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47. A Light‐Induced Decarboxylative‐Elimination of Substituted Maleimides as a Strategy Towards Triggered Photorelease.
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Malde, Roshni, Richards, Daniel A., Salerno, Luigia, Zhao, Yanbo, Karu, Kersti, Chudasama, Vijay, and Baker, James R.
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MALEIMIDES ,NUCLEOPHILIC substitution reactions ,ELIMINATION reactions ,DOUBLE bonds ,CARBOXYLIC acids - Abstract
Herein we report on photodecarboxylations of various substituted maleimides, resulting in an elimination reaction. Furthermore, we establish facile wavelength tunability through modulation of the maleimide double bond substituents. We envisage that these versatile reagents, which are readily constructed and diversified by nucleophilic substitution reactions on bromomaleimides, will offer new opportunities for triggered photorelease. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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48. Single-cell phenotypes of peripheral blood im- mune cells in early and late stages of non-alcoholic fatty liver disease.
- Author
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Waller, Kathryn Jane, Saihi, Hajar, Wenhao Li, Brindley, James Hallimond, Anja De Jong, Wing-kin Syn, Bessant, Conrad, and Alazawi, William
- Published
- 2023
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49. Psoriasisarthritis: Klinische Herausforderungen und medikamentöses Management.
- Author
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Dauth, Stephanie, Klippstein, Maximilian, and Köhm, Michaela
- Abstract
Copyright of Zeitschrift für Rheumatologie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
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50. The Roles of Myeloid Cells in Aging-related Liver Diseases.
- Author
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Jinghao Yao, Yang Li, and Hua Wang
- Published
- 2023
- Full Text
- View/download PDF
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