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Abstract Background Sunflower (Helianthus annuus) is one of the most important economic crops in oilseed production worldwide. The different cultivars exhibit variability in their resistance genes. The NAC transcription factor (TF) family plays diverse roles in plant development and stress responses. With the completion of the H. annuus genome sequence, the entire complement of genes coding for NACs has been identified. However, the reference genome of a single individual cannot cover all the genetic information of the species. Results Considering only a single reference genome to study gene families will miss many meaningful genes. A pangenome-wide survey and characterization of the NAC genes in sunflower species were conducted. In total, 139 HaNAC genes are identified, of which 114 are core and 25 are variable. Phylogenetic analysis of sunflower NAC proteins categorizes these proteins into 16 subgroups. 138 HaNACs are randomly distributed on 17 chromosomes. SNP-based haplotype analysis shows haplotype diversity of the HaNAC genes in wild accessions is richer than in landraces and modern cultivars. Ten HaNAC genes in the basal stalk rot (BSR) resistance quantitative trait loci (QTL) are found. A total of 26 HaNAC genes are differentially expressed in response to Sclerotinia head rot (SHR). A total of 137 HaNAC genes are annotated in Gene Ontology (GO) and are classified into 24 functional groups. GO functional enrichment analysis reveals that HaNAC genes are involved in various functions of the biological process. Conclusions We identified NAC genes in H. annuus (HaNAC) on a pangenome-wide scale and analyzed S. sclerotiorum resistance-related NACs. This study provided a theoretical basis for further genomic improvement targeting resistance-related NAC genes in sunflowers.

As education advances with the times, traditional English EFL teaching can no longer fully adapt to the current educational situation. This paper studies the mining of the Civics element in English teaching integrates it into EFL classroom teaching, and constructs the morality cultivation EFL critical thinking ability teaching strategy based on the critical thinking ability model. The effects of applying this teaching strategy are analyzed and compared to the six dimensions of critical thinking ability: interpretation, analysis, evaluation, reasoning, explanation, and self-regulation ability. The exploration of critical thinking ability development and individual teacher factors is conducted. After the teaching experiment, the experimental group improved by 12.08 points, while the control group only improved by 0.46 points, indicating a significant difference between the two groups’ scores. The Sig. The values of the experimental group in the six dimensions are less than 0.05, and the Sig. The values of the control group are greater than 0.05. The teaching strategy of moral cultivation in EFL critical thinking ability has an obvious effect on improving students’ critical thinking abilities. There is a correlation between the development of critical thinking ability and teachers’ education, and teachers with master’s degrees are better than teachers with bachelor’s degrees.

CircRNAs have been proven to play a pivotal role in cancer progression. The present study aims to explore the roles and related mechanisms of circSETD2 in breast cancer proliferation, migration and invasion. The expression of circSETD2 in BC was assessed by the GEO database and qRT‒PCR. The biological function and underlying molecular mechanism of circSETD2 in BC were explored using in vitro and in vivo experiments, including CCK8, transwell, RIP, western blot, and xenograft mouse models. The expression of circSETD2 was downregulated in BC tumors, in accordance with the GEO database. Overexpression of circSETD2 significantly suppressed cell growth, cell migration and invasion. Mechanistically, circSETD2 reduced the stabilization of YAP1 by competitively binding with HuR, resulting in inactivation of downstream targets such as CTGF, myc and Slug. Our work suggests that the novel signaling axis circSETD2/HuR/YAP1 plays an important role in BC progression. The molecular mechanism underlying this signaling axis may provide a potential therapeutic target for BC treatment.

We establish the formulation for quantum current. Given a symmetry group G, let $\mathcal{C}$:=Rep G be its representation category. Physically, symmetry charges are objects of $\mathcal{C}$ and symmetric operators are morphisms in $\mathcal{C}$. The addition of charges is given by the tensor product of representations. For any symmetric operator O crossing two subsystems, the exact symmetry charge transported by O can be extracted. The quantum current is defined as symmetric operators that can transport symmetry charges over an arbitrary long distance. A quantum current exactly corresponds to an object in the Drinfeld center $Z_1(\mathcal{C})$. The condition for quantum currents to be \TL{superconducting} is also specified, which corresponds to condensation of anyons in one higher dimension. To express the local conservation, the internal hom must be used to compute the charge difference, and the framework of enriched category is inevitable. To illustrate these ideas, we develop a rigorous scheme of renormalization in one-dimensional lattice systems and analyse the fixed-point models. It is proved that in the fixed-point models, superconducting quantum currents form a Lagrangian algebra in $Z_1(\mathcal{C})$ and the boundary-bulk correspondence is verified in the enriched setting. Overall, the quantum current provides a natural physical interpretation to the holographic categorical symmetry.