1. Metabolomic profiling of COVID-19 using serum and urine samples in intensive care and medical ward cohorts
- Author
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Ana Isabel Tristán, Cristina Jiménez-Luna, Ana Cristina Abreu, Francisco Manuel Arrabal-Campos, Ana del Mar Salmerón, Firma Isabel Rodríguez, Manuel Ángel Rodríguez Maresca, Antonio Bernardino García, Consolación Melguizo, Jose Prados, and Ignacio Fernández
- Subjects
COVID-19 ,Metabolomics ,NMR ,Serum ,Urine ,Biomarkers ,Medicine ,Science - Abstract
Abstract The COVID-19 pandemic remains a significant global health threat, with uncertainties persisting regarding the factors determining whether individuals experience mild symptoms, severe conditions, or succumb to the disease. This study presents an NMR metabolomics-based approach, analysing 80 serum and urine samples from COVID-19 patients (34 intensive care patients and 46 hospitalized patients) and 32 from healthy controls. Our research identifies discriminant metabolites and clinical variables relevant to COVID-19 diagnosis and severity. These discriminant metabolites play a role in specific pathways, mainly “Phenylalanine, tyrosine and tryptophan biosynthesis”, “Phenylalanine metabolism”, “Glycerolipid metabolism” and “Arginine and proline metabolism”. We propose a three-metabolite diagnostic panel—comprising isoleucine, TMAO, and glucose—that effectively discriminates COVID-19 patients from healthy individuals, achieving high efficiency. Furthermore, we found an optimal biomarker panel capable of efficiently classify disease severity considering both clinical characteristics (obesity/overweight, dyslipidemia, and lymphocyte count) together with metabolites content (ethanol, TMAO, tyrosine and betaine).
- Published
- 2024
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