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Copyright of Fogorvosi Szemle is the property of Hungarian Dental Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Long-term photo-acoustic stimulation is used for the induction of altered states of consciousness for both therapeutic and experimental purposes. Long-term photo-acoustic stimulation also leads to changes in the composition of saliva which have a key contribution to the efficiency of this technique in easing mucosal symptoms of oral psychosomatic patients. The aim of this study is to find out whether there is any cumulative effect of repeated stimulation and whether there are any detectable differences between diverse stimulatory patterns of long lasting photo-acoustic stimulation on the phenomenology of the appearing trance state and on salivary secretion. There was significant cumulative effect in relation with the appearance of day dreaming as phenomenological parameter, and in relation with protein output and amylase/protein ratio as salivary parameter. Pattern specific effect was detectable in relation with salivary flow rate only. Although our results clearly indicate the existence of certain cumulative and stimulation-pattern specific effects of repeated photo-acoustic stimulation, the absolute values of all these effects were relatively small in this study. Therefore, in spite of their theoretical importance there are no direct clinical consequences of these findings. However, our data do not exclude at all the possibility that repeated stimulation with other stimulatory parameters may lead to more pronounced effects. Further studies are needed to make clear conclusion in this respect., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

The gustatory system plays a critical role in determining food preferences and food intake, in addition to nutritive, energy and electrolyte balance. Fine tuning of the gustatory system is also crucial in this respect. The exact mechanisms that fine tune taste sensitivity are as of yet poorly defined, but it is clear that various effects of saliva on taste recognition are also involved. Specifically those metabolic polypeptides present in the saliva that were classically considered to be gut and appetite hormones (i.e., leptin, ghrelin, insulin, neuropeptide Y, peptide YY) were considered to play a pivotal role. Besides these, data clearly indicate the major role of several other salivary proteins, such as salivary carbonic anhydrase (gustin), proline-rich proteins, cystatins, alpha-amylases, histatins, salivary albumin and mucins. Other proteins like glucagon-like peptide-1, salivary immunoglobulin-A, zinc-α-2-glycoprotein, salivary lactoperoxidase, salivary prolactin-inducible protein and salivary molecular chaperone HSP70/HSPAs were also expected to play an important role. Furthermore, factors including salivary flow rate, buffer capacity and ionic composition of saliva should also be considered. In this paper, the current state of research related to the above and the overall emerging field of taste-related salivary research alongside basic principles of taste perception is reviewed.

The aim of the authors was to investigate whether living as a minority has an influence on the dental fear and anxiety values. In this study 201 volunteers (n = 201, inside border Hungarians 144, outside border Hungarians 57, male 90, female 111; age 8 to 83 years, mean 44 +/- 16 yrs.) were investigated. Our methods included collection of demographic data (gender, age, marital status, profession), and administration of the Hungarian versions of dental fear and anxiety related scales namely: DAS, DAQ, DASQ, DFS, DBS, STAI-S, STAI-T and Expectation Scale. Mean values of the scales were: DAS: 10,34 +/- 3,54; DAQ: 2,3 +/- 1,15; DASQ: 12,58 +/- 4,55; DFS: 40,37 +/- 15,67; DBS: 32,89 +/- 12,94; Expectation Scale: 2,87 +/- 3,56, STAI-S: 39,51 +/- 10,68; STAI-T 41,65 +/- 9,08. The mean scores of all the scales were higher in the case of Hungarians living inside the borders of Hungary. The differences were significant in the case of DAS, DAQ, DASQ and DFS scales (p < 0,05). Data of our study indicate that living as a minority not necessarily leads to the increase of dental fear and anxiety.

There are numerous defense proteins present in the saliva. Although some of these molecules are present in rather low concentrations, their effects are additive and/or synergistic, resulting in an efficient molecular defense network of the oral cavity. Moreover, local concentrations of these proteins near the mucosal surfaces (mucosal transudate), periodontal sulcus (gingival crevicular fluid) and oral wounds and ulcers (transudate) may be much greater, and in many cases reinforced by immune and/or inflammatory reactions of the oral mucosa. Some defense proteins, like salivary immunoglobulins and salivary chaperokine HSP70/HSPAs (70 kDa heat shock proteins), are involved in both innate and acquired immunity. Cationic peptides and other defense proteins like lysozyme, bactericidal/permeability increasing protein (BPI), BPI-like proteins, PLUNC (palate lung and nasal epithelial clone) proteins, salivary amylase, cystatins, prolin-rich proteins, mucins, peroxidases, statherin and others are primarily responsible for innate immunity. In this paper, this complex system and function of the salivary defense proteins will be reviewed.

In the presented case extensive tooth damage has been caused by an eating disorder (bulimia nervosa coupled with frequent vomiting). Although the patient's premised disorder has been treated successfully, palatal and occlusal surfaces of the upper teeth were profoundly damaged due to previous acidic erosion. There was also a significant hypersensitivity of upper teeth, and the patient was dissatisfied with the aesthetics of the upper teeth as well. In order to treat pain symptom, replace lost hard dental tissues, and restore aesthetics, porcelain covered zirconium dioxide crowns of upper teeth were prepared. Tooth hypersensitivity disappeared as a result of the insertion of crowns following a careful minimal-invasive preparation. Patient's high aesthetic needs were also fulfilled by using high quality materials. At the 6-months-follow-up examination disappearance of tooth sensitivity and satisfaction with the aesthetics were reported by the patient. Long run gingival and mucosal health as well as proper condition of dental hard tissues and dentures were determined by the intraoral control examination. The patient also presented high level of oral hygiene.

Aim: To investigate if there is an influence of being a disabled people (with disturbed body-image), dental fear and anxiety on several form/shape parameters of drawings., Subjects: Disabled (46) and healthy (33) volunteers (n = 79; male 39, female 40; age: 18.16 +/- 3.01 yrs.) were investigated., Methods: Collection of demographic data (gender, age, marital status, profession), and administration of the Hungarian versions of dental fear and anxiety related scales namely: DAS, DAQ, DASQ, DFS, DBS, STAI-S, STAI-T and Expectation scale. Free drawing test portraying a person, a mouth, and a tooth were also administered, and form/shape parameters were analyzed., Results: Mean values of the scales were: DAS: 10.22 +/- 3.1; DAQ: 2.21 +/- 0.9; DASQ: 12.44 +/- 3.9; DFS: 40.98 +/- 14,0; Expectation Scale: 2.32 +/- 0.7, DBS: 34.50 +/- 9.0; STAI-S: 37.56 +/-10.7; STAI-T 42.41 +/-10.2. Disables subjects scored higher in all of the scales but there was a significant difference (t-probe, p < 0.05) in the case of DBS scale only. Being a disabled influenced 2 of person-, 5 of tooth-, and 3 of mouth-drawing parameters significantly (chi2 probe, p < 0.05). Dental fear related scales influenced 5 of person-, 5 of tooth-, and 1 of mouth-drawing parameters significantly (one-way ANOVA, p < 0.05). Anxiety influenced 2 of person-, 1 of tooth-, and none of mouth-drawing parameters significantly (one-way ANOVA, p < 0.05)., Conclusion: Interestingly, the influence of being a disabled people was the strongest in case of tooth-drawings, and the influence of dental fear on drawing parameters was stronger than that of anxiety.

Spondyloarthropathies consist of chronic inflammatory disorders genetically linked with each other through HLA-B27 molecules, and are connected with the destruction of periarticular bone and also with systemic bone loss in many cases. Expected molecular mechanisms behind these conditions overlap the functions of Hsp70s, a group of major molecular chaperones and cytokines. Hsp70s may control disease progression via inhibition of unfolded HLA-B27 protein accumulation and alteration of ER stress signaling. Further, Hsp70s may improve disease related malfunction of antigen presentation, and may induce nitric oxide (NO) release from macrophages which probably protective against spondyloarthropathies as well. Considering premised possible influence of Hsp70s on core mechanisms of spondyloarthropathies it may be expected that, increased expression of Hsp70s advantageously retards disease progress, or may lead to remission. On the other hand Hsp70s as danger signals induce the secretion of proinflammatory cytokines playing major role in the progression of spondyloarthropathy induced bone loss. Consequently, the effect of Hsp70s on the progression of spondyloarthropathic bone loss is "Janus-faced" in some respect: increase of Hsp70s' level is likely advantageous regarding to the core of disorder; but it may facilitate existing bone resorption processes.

There is an increasingly growing interest world-wide for the genomics, transcriptomics and proteomics of saliva and the oral cavity, since they provide a non-invasive source of unprecedently rich genetic information. The complexity of oral systems biology goes much beyond the human genome, transcriptome and proteome revealed by oral mucosal cells, gingival crevicular fluid, and saliva, and includes the complexity of the oral microbiota, the symbiotic assembly of bacterial, fungal and other microbial flora in the oral cavity. In our review we summarize the recent information on oral genomics, transcriptomics and proteomics, of both human and microbial origin. We also give an introduction and practical advice on sample collection, handling and storage for analysis. Finally, we show the usefulness of salivary and oral genomics in early diagnosis of cancer, as well as in uncovering other systemic diseases, infections and oral disorders. We close the review by highlighting a number of possible exploratory pathways in this emerging, hot research field.

The possible influence of fear of death and attitude toward death were studied related to dental anxiety in Hungarian elementary and secondary school subjects (n = 277; 114 males, 163 females; age between 8 and 18 years). Dental fear and anxiety scores were DAS: 10.8 +/- 3.6; DFS: 40.6 +/- 15.6; STAI-S: 38.0 +/- 11.0; STAI-T: 40.3 +/- 10.0. Lester's Attitude Toward Death Scale scores were 6.3 +/- 1.3. Girls scored higher on DAS, STAI-S, and STAI-T scales (P < or = 0.05). Age influenced STAI-S, STAI-T, and Lester's Scale scores (P < or = 0.05). Lester's Scale scores influenced the expectations of the subjects about the dental fear of their surrounding people (parents, brother, sister, friends) (P < or = 0.05). A percentage of 7.22 of the subjects indicated a rather strong connection between dental fear and fear of death. These subjects had significantly higher dental fear and anxiety scores as compared to others (P < or = 0.01). Death-related content was found in 4.3% of drawings and in 10.5% of free associations (couplings) related to teeth (in 12.6% either in drawings or in couplings). The appearance of death-related content was higher with higher age, and higher expected dental fear of surrounding people (P < or = 0.01). Our data indicate a detectable influence of fear of death on dental fear, especially in subjects with higher dental fear scores.

Molecular chaperones were considered to be intracellular, but there is increasing evidence demonstrating their cytoprotective and immune modulator properties outside the cell. The major extracellular chaperone (Hsp70) was also found in saliva, indicating a possible effect of Hsp70 on mucosal surfaces. Here we summarize the immune-modulatory role of the 70-kDa stress protein family, with special attention on the potential impact of salivary Hsp70 on oral defense mechanisms. There are three major facets of Hsp70-induced immune activation: 1) the appearance of Hsp70 on the surface of certain tumor cells or virally infected cells, leading to their phagocytosis and subsequent lysis; 2) the role of extracellular uncomplexed Hsp70 as a danger signal, leading to the secretion of proinflammatory cytokines from antigen-presenting cells and T lymphocytes and of nitric oxide from macrophages as well as to complement activation; 3) receptor-mediated uptake of peptide-loaded Hsp70 to antigen-presenting cells and cross-presentation of the Hsp70-peptide complex as an antigen to cytotoxic T cells and natural killer lymphocytes. The immune-activating effect of salivary Hsp70 may also be highly important in oral defense, especially in areas where molecular and cellular participants of the immune response appear on the surface of the oral cavity (i.e. several lesions of the mucosa and the periodontal tissues).

Copyright of Fogorvosi Szemle is the property of Hungarian Dental Association and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The connection between unconscious subjects and psycho-somatic manifestation is well known in the literature. Although more and more data is known about this connection related to the oro-facial region as well, investigation of this interesting problem is still has theoretical and clinical relevance. To collect more data about this field, 257 of tooth drawings of 8-18 years old schoolchildren from normal population were studied as a pilot to establish further investigations related to drawing-projections in connection with the oro-facial region. This first analysis of the data indicated that, gender correlates significantly with the size of the drawings (p< or =0.05), and localisation of the drawings (p< or =0.05). Differences between elementary schoolchildren and the more mature middle school children were detected as well related to subject (p< or =0.05) and localisation (p< or =0.05) of the drawings.

Free association (coupling) of 97 Hungarian primary school children (age: 8-15 yrs, 44 male, 53 female) about their teeth was collected and analysed related to lexicologic parameters, as a pilot to establish further investigations. In some cases significant (p < or = 0.05) differences within the groups related to several topics were detected in the case of the length of the text and in the case of the distribution of etymons (root of word). Gender significantly influenced the length of the text as well. Some effect of dental fear and anxiety on the length of the text, and on the etymon's distribution may also be possible. The analysis of the most frequently used words indicated some coupling of pain and fear, and the importance of the mother in how the children see dental life events.

In this study 549 school children of the Hungarian minority in Transylvania were investigated (n=549, 342 female, 207 male, age between: 12-19 years). Mean dental fear scores were high: DAS: 12.6 +/- 3.3, DFS: 50.6 +/- 25.1. Girls scored significantly higher (p< or =0.01) in both scales. Scores increased between age of 12 and 16 confluently in both scales (DAS, DFS; p< or =0.05). There was a strong Pearson's correlation between DAS and DFS scales: (r= 0.70; p< or =0.01), and a somewhat lower correlation between these scales and the general anxiety scores. Mean DBS score (n = 203 only) was: 44.9 +/- 10.2.

Although there were drastic changes in the XX-th century, still 70% of the Hungarian population believe in God, and 10-15% can be counted as active believer. A percentage of 44% of the hospitalised patients indicated a need of pastoral counseling during the treatment time. In the field of psychiatry and psychotherapy the percentage of the need of religious care (pastoral psychology, pastoral counseling) may be even higher. In the field of dentistry the increasing number of psychosomatic patients justify the introduction of such religious treatment methods into the dental care system as well. In this review authors try to collect the main points of this special, religious type of therapy to help dental professionals working in the field of psychosomatic dentistry in the orientation in this interesting and important field.

Free association (coupling) of 139 Hungarian secondary school students (90 females, 49 males, age between 14-18 yr.) about their teeth was collected. Dental fear (DAS, DFS) and general anxiety scores were measured. Typical dental events (i.e.: simple and traumatising dental treatments, etc.) were coupled by the participants in 36,7% of the cases. Functions and importance of the teeth and oral hygiene were described in 7,2% of the cases. Simple, grotesque, or magical stories and tales about teeth were found in 16,5% of the cases. No answer was found in 39,6% of the cases. Highest dental fear and general anxiety scores were found in the group coupled traumatising dental treatment. Age had no influence on the sense of the association (coupling).

Saliva plays an important role in oral health, regulating and maintaining the integrity of the oral hard tissues and soft tissues. This paper reviews the role of saliva in the oral defence mechanisms. The most important salivary immune and non-immune defence proteins and constituents that take part in the pellicle formation and re-mineralisation processes are introduced. General health conditions, medications and salivary gland disorders influencing the defence potential of the saliva are discussed. Treatment possibilities of such conditions, and the future perspective of saliva research is also presented.

25 "problem-patients" of the Clinic of Prosthetic Dentistry participated in this study (21 female, 4 male, aged between 32-81 yr.; mean age: 56.92+/-12.09). All included therapies were provided by dentists specialised in psychotherapy, and the efficacy of our model to treat denture intolerance patients was analysed based on the time need, and other parameters. Psychiatric diagnostic (ICD-10) categories were: F20-29: 12%; F30-39: 40%, F40-48: 36%; F60-69: 12%. The efficacy of the provided psychotherapy was: recovery in 44% (patient accepted the denture); improvement in 32% (patient compromised to accept the denture); no effect in 24% (the patient did not accept the denture). Number of psychotherapeutic sittings ranged: 1-10 in 24%; 10-20 in 28%, 20-30 in 16%, 30-40 in 8%; 40-50 in 8%; 50-60 in 12%; and 80-90 in 4% of the cases. Our results indicate that, the efficacy of dentists specialised in psychotherapy is appropriate to treat denture intolerance patients. However, treatments are rather time consuming, and 24% of the cases are unsuccessful. Because of this, the prevention and early diagnosis of this problems are crucial.

Free associations (coupling) of 139 Hungarian primary school children about their teeth was collected. Dental fear (DAS, DFS) and general anxiety scores were measured. Typical dental events (i.e.: loss of deciduous teeth, simple and traumatising dental treatments, tooth fractures) were coupled by the participants in 41.0% of the cases. Functions and importance of the teeth and oral hygiene were described in 20.1% of the cases. Simple, grotesque, or magical stories and tales about teeth were found in 8.6% of the cases. No answer was given in 30.2% of the cases. Highest dental fear and general anxiety scores were found in the group coupled traumatising dental treatment. Traumatising loss of deciduous teeth was caused by the dentist or by the father of the child, and was associated with higher dental fear and general anxiety comparing to simple loss of deciduous teeth. Higher dental fear and general anxiety scores were found in the group coupled functions and importance of the teeth comparing to the group coupled simple, grotesque, or magical stories and tales, or the group giving no answer.

100 dental patients waiting for hypnotic dental treatment (n = 100, 58 female, 42 male, mean age: 36.4 +/- 10.6 yr.) was investigated about their perceived origins of dental anxiety. Dental anxiety levels (DAS, DFS) and general anxiety were measured as well. Mean dental anxiety scores were high (DAS: 12.5 +/- 3.3; DFS: 47.9 +/- 17.3). The most frequent reason of high dental anxiety was previous painful dental treatment (20.0%), dislike of dentist's behaviour (15.0%), treatment error (5.0%), and "other reasons" (4.0%). A large amount of the patients (48.0%) did not know what to expect, and 8.0% indicate no fear related to dentistry. Previous painful dental treatment induced the highest dental anxiety (DAS: 15.1 +/- 3.1; DFS: 58.1 +/- 20.3), followed by the "other reasons" (DAS: 14.0 +/- 0.8; DFS: 50.5 +/- 13.5), treatment error (DAS: 13.0 +/- 3.7; DFS: 49.0 +/- 16.1), and dislike of dentist's behaviour (DAS: 11.4 +/- 2.8; DFS: 45.0 +/- 12.5). Increased general anxiety was found in the groups indicated previous painful dental treatment, "other reasons", and no expectation.

Long-term photo-acoustic stimulation leads to changes in the composition of saliva, which may have a key contribution to the effectivity of this technique in easing mucosal symptoms of psychosomatic patients. In the present study a significant (P

Authors describe a 58-sitting psychotherapy of a 46-year-old female patient with psychogenic atypical facial pain. Sexual trauma in the childhood was found as a cause of the psychogenic pain symptoms, leading to series of unnecessary extraction of teeth in this case. Psychotherapeutic treatment led to the recovery of the somatic symptoms, and a moderate improvement of the behaviour related to men of this patient.

Authors investigated the dental fear scores (DAS, DFS) and anxieties of 362 persons from several sub-populations living in Budapest. The subjects were: 253 females, 109 males, aged 14 to 73. Dental fear scores were rather high (DAS: 11.0 +/- 3.6; DFS: 42.0 +/- 16.0). Age influenced the dental fear scales (DAS and DFS) only; sex, and marital status influenced both dental fear scales and general anxiety scales (one-way ANOVA). A strong correlation between DAS and DFS, and somewhat lower correlation between these scales and the general anxiety scales were also demonstrated.

The Hungarian version of DBS was analysed on 100 participants (64 females, 36 males, mean age: 36.4 +/- 14.3) from 5 subgroups: dental students (n = 20), other intellectuals (n = 20), dental nurses (n = 19), dental patients (n = 20), family doctor's patients (n = 21). Mean values were: DBS: 29.1 +/- 10.8; DAS: 10.6 +/- 3.9; DFS: 39.0 +/- 16.7. Family doctor's patients scored higher (p < or = 0.05) on DBS than all other groups, and dental students scored lower on DBS than all other groups (p < or = 0.05; except other intellectuals). DBS scores of age group between 21-30 yrs. differed significantly from age groups 31-40 yrs. (p < or = 0.05) and 41-50 yrs. (p < or = 0.01). There were no differences related to gender and marital status related to DBS scores. Positive Pearson's correlation (p < or = 0.05) of DBS was stronger related to dental anxiety scores (DAS: r = 0.56, DFS: r = 0.63) than related to general anxiety scores, whereas Pearson's correlation between DAS and DFS scores was even more prominent (r = 0.82, p < or = 0.05).

The frequency of occurrence of amnesia, analgesia and time distortion during hypnotic dental treatments (n = 60) was investigated on high dental anxiety patients. Hypnosis with and without standardised direct suggestions related to amnesia, analgesia and time distortion were compared. Treatment of alert patients without direct suggestions (n = 10) were also used for comparison. Amnesia and time distortion was higher (p < or = 0.05) with the use of suggestions under hypnotic conditions, but analgesia was not significantly different. Alert appearance of the events were in all cases less (p < or = 0.01) than under hypnotic conditions.

Effect of photo-acoustic stimulation on the flow rate and protein concentration of whole saliva was investigated. 10 medical students' and 11 edentulous patients' salivary volume and protein concentrations were measured before, during, and after stimulation. The flow rate of the students' group was significantly higher (p < or = 0.01) before and after the treatment, whereas the protein concentration was significantly lower (p < or = 0.05) before, during and after treatment comparing to the patients' group. The flow rate of the students' groups significantly decreased during stimulation (p < or = 0.05). Salivary protein concentration of the students' group significantly increased (p < or = 0.05) after stimulation. There were no significant changes in the group of patients. Repeated stimulation combined with hypnotic relaxation was used in the case of 4 psychosomatic patients. Resting salivary flow and protein concentration significantly increased in 2 cases (p < or = 0.05) as a result of the therapy.

Dental treatments of high dental anxiety patients (n = 20) under hypnotic conditions were investigated. Time need of main hypnotic events like rapport; induction: deepening; calming: developing imagination; dehypnosis; discussion was measured. Total time was significantly higher (p < or = 0.05) than pure dental treatment time, but the time the patients percepted was significantly less then total (p < or = 0.05), even somewhat less then pure dental treatment time. Time distortion correlated significantly (negative Pearson's correlation; p < or = 0.05) with the time need of rapport (-0.50), calming (-0.51) and dehipnosis (-0.46), and with the time need of developing delightful imagination (+0.57) (positive Pearson's correlation; p < or = 0.05). There was no significant correlation between time distortion and the time need of hypnotic induction, deepening, total time need of hypnosis, or pure dental treatment time.

The possible use of a modified photo-acoustic stimulation technique was investigated in the explorative part of the psychotherapy of atypical oro-facial pain patients (n = 20; 13 females, 7 males; age between 39-59; psychiatric diagnostic categories in ICD-10: F41.2, F45.1) Since the stimulatory effects of this method cause difficulties to induce free imaginations, previously the main indication of this technique was the ego-strengthening of the patients, to help them to recognise and to verbalise emotions. In the modified technique, specific suggestions coming from detected changes of the patients' emotions, their symptoms, or their social activities are used. Our early results suggest that the modified technique advantageously speeded up the therapeutic process, but did not change the final therapeutic outcome.

Authors investigated the dental fear scores (DAS, DFS) of 139 primary school children in Budapest, Hungary (72 females, 67 males, ages between 8-15 years). Sex and age had no effect on the scales. Mean dental fear scores were high (DAS: 10.7 +/- 3.7, DFS: 40.4 +/- 15.3). A strong correlation between DAS and DFS, and somewhat lower correlation between these scales and the general anxiety scales were demonstrated. Children's evaluation of the dental fear of the family and relations strongly correlated with dental fear and moderately with general anxiety. The results indicate that, dental fear is influenced by dental fear of family and relations, and general anxiety, but much less influenced by other demographic variables (i.e. age, sex) in primary-school children.

Authors investigated the Dental Fear Survey scores (DFS) of 362 persons from several subpopulations living in Budapest. Subjects were: 253 females, 109 males, aged 14 to 73 years. Scores of the items related to dental handpiece and anaesthetic needle were the highest. The group the participants belonged to influenced 19 items, marital status and age influenced 13 items, and sex influenced 10 items of the DFS significantly (one-way ANOVA, p < or = 0.05). Scores related to dental handpiece were higher than scores related to the anaesthetic needle, and the scores of the 20th item ("overall fear of dentistry") were near to the scores of most fearful items, indicating some "phobic character" of the Hungarian population.

Background: There is increasing evidence that chaperones are also present outside the cell, exerting cytokine-like effects and influencing immune recognition. Hsp70 has been found to be present in human blood sera. Chaperonins Cpn10 and Cpn60 are present in pancreatic juice, but Hsp70 is not. These observations raise the possibility that molecular chaperones may be present in other secretory fluids, such as human saliva., Material/methods: Human whole saliva was collected from six participants under resting conditions and secretory stimulation. The samples were precleared by centrifugation and sterile filtered. Salivary volume, protein concentration and amylase activity were determined. For detection of Hsp70 saliva proteins were separated on a 12.5% SDS-PAGE. Semi-dry Western blot analysis was used with a primary antibody against the inducible form of Hsp70. Hsp70 bands were detected with a horseradish peroxidase-linked secondary antibody and ECL-Western blotting analysis., Results: A single band was recognized around 70 kDa in the saliva of all the participants. There was a significant decrease of Hsp70, and a non-significant decrease of total protein concentration during stimulation, whereas the activity of salivary amylase increased significantly. Stimulation significantly increased the Hsp70, total protein and amylase outputs as well as the amylase/protein ratio, and decreased the Hsp70/amylase and Hsp70/protein ratios., Conclusions: Hsp70 is secreted to saliva, but unlike amylase is not transported by the exocytotic secretory mechanisms of acinar cells. Passive transport mechanisms of Hsp70 from blood serum or from salivary gland cells may be major routes of salivary Hsp70 secretion.

The overactive gag reflex is one of the etiologic categories of psychosomatic symptoms, which most often arise from environmental stressors. If organic disturbances, anatomic anomalies, or biomechanical inadequacies of existing prostheses are not key causes, the services of trained specialists are needed to help with behavioural management of the problem. Hypnosis can provide the clinician with a set of techniques, which may be used to augment or facilitate a particular course of treatment. In the case report, the patient's history and her overactive gag reflex suggested to use hypnosis therapy. The responsibility of a dentist can be found in his possible recognition of eventually necessary psychotherapy when consulting a patient.

The specificity of the oral psychogenic manifestations is based on the unique psychological and depth-psychological function of the mouth, and teeth during infancy (breasting), sexuality, and aggressivity. Fixed dentures, implants, and especially removable dentures can modify strongly these psychological functions and activate oral fixations leading to psychogenic symptoms. In the present paper the great importance of the oral phase of the development of personality and the symbolical values of the mouth, teeth, tongue, and face in the oro-facial conversive symptom manifestations are discussed.

Authors present a case report of an adult patient treated with the combination of orthodontic and prosthetic treatment methods. The importance of periodontal and psychological management of such patients is pointed out.

Photo-acoustic stimulation provides a powerful stimulation of the central nervous system, whereas it relaxes the body. Authors found it useful to eliminate simple anxiety, and to reduce salivary secretion in the oral cavity. The combination of photo-acoustic stimulation with hypnotic techniques has been found as an excellent method to treat oro-facial psychogenic symptoms and denture intolerance. In the case of odonto-phobic patients, the combined method has been found less effective than hypnotic techniques alone.

Authors translated the "Dental Fear Survey" (DFS) into Hungarian. 196 persons have been investigated and the DFS values have been compared to DAS, STAI-S, STAI-T values. Mean values were: DFS: 46.27; DAS: 12.24; STAI-S: 41.58; STAI-T: 42.68. Authors found all values higher in the case of women comparing to men. Positive correlation has been found between DAS and DFS, but STAI-S and STAI-T increased only moderately comparing to the DAS and DFS values.

Authors translated the Corah's "Dental Anxiety Scale" (DAS) into Hungarian. 143 dental patients, and 74 dental student's, were investigated and the DAS values were compared to the State-Trait Anxiety Invertory (STAI-S and STAI-T) values. Mean values were: DAS: 9.92, STAI-S: 39.17, STAI-T: 41.34. Authors found lower scores in the case of dental students comparing to patients (p < 0.001), and women comparing to men (p < 0.001). DAS values increased slowly with age, and decreased at 60 years old, or older patients. Married patients had higher DAS and STAI-S scores, but lower STAI-T scores comparing to the unmarried and divorced patients.

The frequency of occurrence of spontaneous amnesia analgesia and time distortion was investigated in 42 cases during dental treatment carried out under hypnosis. Amnesia occurred in 53.5% analgesia in 57.8% and time distortion in 64.0%.

The use of meditative state for hypnotic induction in the dental practice was investigated in 45 cases. In 38 cases (84.4%) the hypnosis was found as a useful additional method. It was especially useful to reduce anxiety, and in some psychosomatic diseases.

The relationship between dental fluorosis and alterations in the salivary proteome remains inadequately elucidated. This study aimed to investigate the salivary proteome and fluoride concentrations in urine and drinking water among Thai individuals afflicted with severe dental fluorosis. Thirty-seven Thai schoolchildren, aged 6–16, were stratified based on Thylstrup and Fejerskov fluorosis index scores: 10 with scores ranging from 5 to 9 (SF) and 27 with a score of 0 (NF). Urinary and water fluoride levels were determined using an ion-selective fluoride electrode. Salivary proteomic profiling was conducted via LC–MS/MS, followed by comprehensive bioinformatic analysis. Results revealed significantly elevated urinary fluoride levels in the SF group (p = 0.007), whereas water fluoride levels did not significantly differ between the two cohorts. Both groups exhibited 104 detectable salivary proteins. The NF group demonstrated notable upregulation of LENG9, whereas the SF group displayed upregulation of LDHA, UBA1, S100A9, H4C3, and LCP1, all associated with the CFTR ion channel. Moreover, the NF group uniquely expressed 36 proteins, and Gene Ontology and pathway analyses suggested a link with various aspects of immune defense. In summary, the study hypothesized that the CFTR ion channel might play a predominant role in severe fluorosis and highlighted the depletion of immune-related salivary proteins, suggesting compromised immune defense in severe fluorosis. The utility of urinary fluoride might be a reliable indicator for assessing excessive fluoride exposure. [ABSTRACT FROM AUTHOR]

Dental caries is a multifactorial chronic disease resulting from the intricate interplay among acid‐generating bacteria, fermentable carbohydrates, and several host factors such as saliva. Saliva comprises several proteins which could be utilized as biomarkers for caries prevention, diagnosis, and prognosis. Mass spectrometry‐based salivary proteomics approaches, owing to their sensitivity, provide the opportunity to investigate and unveil crucial cariogenic pathogen activity and host indicators and may demonstrate clinically relevant biomarkers to improve caries diagnosis and management. The present review outlines the published literature of human clinical proteomics investigations on caries and extensively elucidates frequently reported salivary proteins as biomarkers. This review also discusses important aspects while designing an experimental proteomics workflow. The protein–protein interactions and the clinical relevance of salivary proteins as biomarkers for caries, together with uninvestigated domains of the discipline are also discussed critically. [ABSTRACT FROM AUTHOR]

Sensory systems mediate our social interactions, food intake, livelihoods, and other essential daily functions. Age-related decline and disease in sensory systems pose a significant challenge to healthy aging. Research on sensory decline in humans is informative but can often be difficult, subject to sampling bias, and influenced by environmental variation. Study of animal models, including mice, rats, rabbits, pigs, cats, dogs, and non-human primates, plays a complementary role in biomedical research, offering advantages such as controlled conditions and shorter lifespans for longitudinal study. Various species offer different advantages and limitations but have provided key insights in geroscience research. Here we review research on age-related decline and disease in vision, hearing, olfaction, taste, and touch. For each sense, we provide an epidemiological overview of impairment in humans, describing the physiological processes and diseases for each sense. We then discuss contributions made by research on animal models and ideas for future research. We additionally highlight the need for integrative, multimodal research across the senses as well as across disciplines. Long-term studies spanning multiple generations, including on species with longer life spans, are also highly valuable. Overall, integrative studies of appropriate animal models have high translational potential for clinical applications, the development of novel diagnostics, therapies, and medical interventions and future research will continue to close gaps in these areas. Research on animal models to improve understanding of the biology of the aging senses and improve the healthspan and additional research on sensory systems hold special promise for new breakthroughs., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to American Aging Association.)

Laryngotracheal aspiration has a widely-held reputation as a primary cause of lower respiratory infections, such as pneumonia, and is a major concern of care providers of the seriously ill orelderly frail patient. Laryngeal mechanical inefficiency resulting in aspiration into the lower respiratory tract, by itself, is not the cause of pneumonia. It is but one of several factors that must be present simultaneously for pneumonia to develop. Aspiration of oral and gastric contentsoccurs often in healthy people of all ages and without significant pulmonary consequences. Inthe seriously ill or elderly frail patient, higher concentrations of pathogens in the contents of theaspirate are the primary catalyst for pulmonary infection development if in an immunocompromised lower respiratory system. The oral cavity is a complex and ever changing eco-environment striving to maintain homogeneity among the numerous microbial communities inhabiting its surfaces. Poor maintenance of these surfaces to prevent infection can result inpathogenic changes to these microbial communities and, with subsequent proliferation, can altermicrobial communities in the tracheal and bronchial passages. Higher bacterial pathogen concentrations mixing with oral secretions, or with foods, when aspirated into an immunecompromised lower respiratory complex, may result in bacterial aspiration pneumonia development, or other respiratory or systemic diseases. A large volume of clinical evidence makes it clear that oral cleaning regimens, when used in caring for ill or frail patients in hospitals and long-term care facilities, drastically reduce the incidence of respiratory infection and death. The purpose of this narrative review is to examine oral health as a required causative companionin bacterial aspiration pneumonia development, and the effectiveness of oral infection control inthe prevention of this disease. [ABSTRACT FROM AUTHOR]

The precise identification of Alzheimer's disease and other prevalent neurodegenerative diseases remains a difficult issue that requires the development of early detection of the disease and inexpensive biomarkers that can replace the present cerebrospinal fluid and imaging biomarkers. Blood biomarkers, such as amyloid and neurofilament light, have been emphasized as an important and practical tool in a testing or examination procedure thanks to advancements in ultra‐sensitive detection techniques. Although saliva is not currently being researched for neurodegenerative diseases, it is an important source of biomarkers that can be used for the identification of diseases and has some advantages over other biofluids. While this may be true for most people, getting saliva from elderly people presents some significant challenges. In this overview, we will first discuss how saliva is created and how aging‐related illnesses may affect the amount and kind of saliva produced. The findings support the use of salivary amyloid protein, tau species, and novel biomarkers in the diagnosis of Alzheimer's disease. [ABSTRACT FROM AUTHOR]

In this review, we address the interplay between the complement system and host microbiomes in health and disease, focussing on oral bacteria known to contribute to homeostasis or to promote dysbiosis associated with dental caries and periodontal diseases. Host proteins modulating complement activities in the oral environment and expression profiles of complement proteins in oral tissues were described. In addition, we highlight a sub-set of bacterial proteins involved in complement evasion and/or dysregulation previously characterized in pathogenic species (or strains), but further conserved among prototypical commensal species of the oral microbiome. Potential roles of these proteins in host-microbiome homeostasis and in the emergence of commensal strain lineages with increased virulence were also addressed. Finally, we provide examples of how commensal bacteria might exploit the complement system in competitive or cooperative interactions within the complex microbial communities of oral biofilms. These issues highlight the need for studies investigating the effects of the complement system on bacterial behaviour and competitiveness during their complex interactions within oral and extra-oral host sites. [ABSTRACT FROM AUTHOR]

In order to explore the potential contributions of the siesta on nocturnal sleeping quality and respiratory immunity under thermoneutral environments, an experimental study was conducted under the thermoneutral environment. In this experiment, 20 subjects were recruited and divided into two groups, one of which had siesta while the other did not but acted as a control group. During the experiment at 25°C, the sleeping patterns, including the slow wave sleeping (SWS) duration, light sleeping (N1, N2 periods) duration, as well as the rapid eye movement (REM) duration, were recorded continuously. Meanwhile, the concentration of the salivary secretory immunoglobulin E (S-IgE), which acted as a biomarker of respiratory immunity, was also measured. Results showed that the mean skin temperature (MST) of the siesta group was 0.37°C lower than that of the control group. In addition, a slight increase (0.1°C) in core temperature was detected in the siesta group. Furthermore, results indicated that both the SWS and REM sleeping durations of the siesta group were reduced, while elevations were observed during the light sleeping duration. However, the subjective evaluation of the nocturnal sleeping quality of the siesta group was improved by 2.9 points. Finally, a higher S-IgE concentration was detected in the siesta group. This study revealed that the siesta might affect the nocturnal sleeping patterns; meanwhile, the changes in S-IgE might correspond to an improvement in respiratory immunity. [ABSTRACT FROM AUTHOR]

Silane chemistry has emerged as a powerful tool for surface modification, offering a versatile means to enhance the properties of various substrates, such as dental implant abutment materials. In this study, we investigated the stability of the 3‐aminopropyldiisopropylethoxysilane (APDS) layer on yttria‐partially stabilized zirconia (Y‐TZP) surfaces after mechanical, acid, and thermal treatment in order to simulate fluctuations within the oral cavity. To accomplish that, the viability of human gingival fibroblasts on APDS‐modified surfaces after applied treatment strategies was assessed by live/dead staining. Moreover, the hydrolysis stability and enzymatic degradation resistance of crosslinked fibronectin to the APDS layer was examined by immunostaining and western blot. The results revealed that the applied modifications were not affected by the different treatment conditions and could withstand the fluctuations in the oral cavity. Furthermore, crosslinked fibronectin on silanized Y‐TZP was stable against hydrolysis over 21 days and enzymatic degradation. We thus can conclude that the proposed functionalization method has high potential to tolerate harmful effects within the oral cavity and remains unchanged on the surface. [ABSTRACT FROM AUTHOR]