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21 results on '"Dudczig, Stefanie"'

1. Unraveling the evolutionary origin of the complex Nuclear Receptor Element (cNRE), a cis-regulatory module required for preferential expression in the atrial chamber

Author

Nunes Santos, Luana, Sousa Costa, Ângela Maria, Nikolov, Martin, Carvalho, João E., Coelho Sampaio, Allysson, Stockdale, Frank E., Wang, Gang Feng, Andrade Castillo, Hozana, Bortoletto Grizante, Mariana, Dudczig, Stefanie, Vasconcelos, Michelle, Rosenthal, Nadia, Jusuf, Patricia Regina, Nim, Hieu T., de Oliveira, Paulo, Guimarães de Freitas Matos, Tatiana, Nikovits, Jr., William, Tambones, Izabella Luisa, Figueira, Ana Carolina Migliorini, Schubert, Michael, Ramialison, Mirana, and Xavier-Neto, José

Published
2024
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2. The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Author

Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan, and Hogan, Benjamin M.

Published
2021
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4. Unraveling the evolutionary origin of the complex Nuclear Receptor Element (cNRE), a cis-regulatory module required for preferential expression in the atrial chamber

Author

Neto, Jose Xavier, primary, Santos, Luana Nunes, additional, Costa, Angela Maria Sousa, additional, Nikolov, Martin, additional, Carvalho, João, additional, Sampaio, Allysson Coelho, additional, Stockdale, Frank, additional, Castillo, Hozana Andrade, additional, Grizante, Mariana Bortoletto, additional, Dudczig, Stefanie, additional, Vasconcelos, Michelle, additional, Rosenthal, Nadia, additional, Jusuf, Patricia, additional, Nim, Hieu, additional, Lopes-de-Oliveira, Paulo, additional, Matos, Tatiana Guimaraes de Freitas, additional, Nikovits, William, additional, Schubert, Michael, additional, and Ramialison, Mirana, additional

Published
2023
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5. Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth, additional, Yu, Hujun, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I, additional, Paterson, Scott, additional, Rondon Galeano, Maria, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne K, additional, Powell, Joseph, additional, Smith, Kelly A, additional, Okuda, Kazuhide S, additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M, additional

Published
2023
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7. Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, Mason, Elizabeth, Yu, Oliver, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Okuda, Kazuhide, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Koltowska, Katarzyna, Hogan, Benjamin M., Grimm, Lin, Mason, Elizabeth, Yu, Oliver, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Okuda, Kazuhide, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Koltowska, Katarzyna, and Hogan, Benjamin M.

Abstract

During development, the lymphatic vasculature forms as a second, new vascular network derived from blood vessels. The transdifferentiation of embryonic venous endothelial cells (VECs) into lymphatic endothelial cells (LECs) is the first step in this process. Specification, differentiation and maintenance of LEC fate are all driven by the transcription factor Prox1, yet downstream mechanisms remain to be elucidated. We present a single cell transcriptomic atlas of lymphangiogenesis in zebrafish revealing new markers and hallmarks of LEC differentiation over four developmental stages. We further profile single cell transcriptomic and chromatin accessibility changes in zygotic prox1a mutants that are undergoing a VEC-LEC fate reversion during differentiation. Using maternal and zygotic prox1a/prox1b mutants, we determine the earliest transcriptomic changes directed by Prox1 during LEC specification. This work altogether reveals new transcriptional targets and regulatory regions of the genome downstream of Prox1 in LEC maintenance, as well as showing that Prox1 specifies LEC fate primarily by limiting blood vascular and hematopoietic fate. This extensive single cell resource provides new mechanistic insights into the enigmatic role of Prox1 and the control of LEC differentiation in development.

Published
2023

8. Author Reply to Peer Reviews of Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth A, additional, Yu, Oliver, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I, additional, Paterson, Scott, additional, Okuda, Kazuhide, additional, Rondon Galeano, Maria, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne, additional, Powell, Joseph, additional, Smith, Kelly A, additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M, additional

Published
2022
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9. Single cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Author

Grimm, Lin, primary, Mason, Elizabeth, additional, Yu, Oliver, additional, Dudczig, Stefanie, additional, Panara, Virginia, additional, Chen, Tyrone, additional, Bower, Neil I., additional, Paterson, Scott, additional, Okuda, Kazuhide, additional, Galeano, Maria Rondon, additional, Kobayashi, Sakurako, additional, Senabouth, Anne, additional, Lagendijk, Anne K., additional, Powell, Joseph, additional, Smith, Kelly A., additional, Koltowska, Katarzyna, additional, and Hogan, Benjamin M., additional

Published
2022
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10. A tripartite structure, the complex nuclear receptor element (cNRE), is a cis-regulatory module of viral origin required for atrial chamber preferential gene expression

Author

Nunes Santos, Luana, primary, Costa, Angela, additional, Nikolov, Martin, additional, Sampaio, Allysson Coelho, additional, Stockdale, Frank, additional, Castillo, Hozana Andrade, additional, Grizante, Mariana Bortoletto, additional, Dudczig, Stefanie, additional, Vasconcelos, Michelle, additional, Rosenthal, Nadia, additional, Jusuf, Patricia Regina, additional, Lopes-de-Oliveira, Paulo, additional, Matos, Tatiana, additional, Nikovits, Wiliam, additional, Schubert, Michael, additional, Ramialison, Mirana, additional, and Xavier-Neto, Jose, additional

Published
2021
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12. The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Author

Hubrecht Institute with UMC, Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan, Hogan, Benjamin M., Hubrecht Institute with UMC, Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan, and Hogan, Benjamin M.

Published
2021

15. The chemokine receptor cxcr5 regulates the regenerative neurogenesis response in the adult zebrafish brain

Author

Kizil Caghan, Dudczig Stefanie, Kyritsis Nikos, Machate Anja, Blaesche Juliane, Kroehne Volker, and Brand Michael

Subjects
Adult zebrafish telencephalon, cxcr5, Radial glia, Proliferation, Regenerative neurogenesis, Adult neurogenesis, Differentiation, Neurology. Diseases of the nervous system, RC346-429
Abstract

Abstract Background Unlike mammals, zebrafish exhibits extensive neural regeneration after injury in adult stages of its lifetime due to the neurogenic activity of the radial glial cells. However, the genes involved in the regenerative neurogenesis response of the zebrafish brain are largely unknown. Thus, understanding the underlying principles of this regeneration capacity of the zebrafish brain is an interesting research realm that may offer vast clinical ramifications. Results In this paper, we characterized the expression pattern of cxcr5 and analyzed the function of this gene during adult neurogenesis and regeneration of the zebrafish telencephalon. We found that cxcr5 was upregulated transiently in the RGCs and neurons, and the expression in the immune cells such as leukocytes was negligible during both adult neurogenesis and regeneration. We observed that the transgenic misexpression of cxcr5 in the ventricular cells using dominant negative and full-length variants of the gene resulted in altered proliferation and neurogenesis response of the RGCs. When we knocked down cxcr5 using antisense morpholinos and cerebroventricular microinjection, we observed outcomes similar to the overexpression of the dominant negative cxcr5 variant. Conclusions Thus, based on our results, we propose that cxcr5 imposes a proliferative permissiveness to the radial glial cells and is required for differentiation of the RGCs to neurons, highlighting novel roles of cxcr5 in the nervous system of vertebrates. We therefore suggest that cxcr5 is an important cue for ventricular cell proliferation and regenerative neurogenesis in the adult zebrafish telencephalon. Further studies on the role of cxcr5 in mediating neuronal replenishment have the potential to produce clinical ramifications in efforts for regenerative therapeutic applications for human neurological disorders or acute injuries.

Published
2012
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17. The Independent Probabilistic Firing of Transcription Factors: A Paradigm for Clonal Variability in the Zebrafish Retina

Author

Boije, Henrik, Rulands, Steffen, Dudczig, Stefanie, Simons, Benjamin D, Harris, William A, Rulands, Steffen [0000-0001-6398-1553], Simons, Benjamin [0000-0002-3875-7071], Harris, William [0000-0002-9995-8096], and Apollo - University of Cambridge Repository

Subjects
Cellbiologi, Stem Cells, Gene Expression Regulation, Developmental, Cell Differentiation, Cell Biology, Zebrafish Proteins, Article, Retina, Animals, Cell Lineage, sense organs, Zebrafish, Transcription Factors, Developmental Biology
Abstract

Summary Early retinal progenitor cells (RPCs) in vertebrates produce lineages that vary greatly both in terms of cell number and fate composition, yet how this variability is achieved remains unknown. One possibility is that these RPCs are individually distinct and that each gives rise to a unique lineage. Another is that stochastic mechanisms play upon the determinative machinery of equipotent early RPCs to drive clonal variability. Here we show that a simple model, based on the independent firing of key fate-influencing transcription factors, can quantitatively account for the intrinsic clonal variance in the zebrafish retina and predict the distributions of neuronal cell types in clones where one or more of these fates are made unavailable., Graphical Abstract, Highlights • A simple quantitative model can explain clonal variability in the retina • This model is based on the firing probabilities of key transcription factors • These probabilities are shown to be largely independent of each other • The environment has only a minor effect on these probabilities, Boije et al. show that a simple model, based on independent and probabilistic firing of key fate-influencing transcription factors, can quantitatively account for intrinsic clonal variance in zebrafish retina. Robust distributions generated through stochastic processes can explain how one retina is almost invariant from the next despite high clonal variability.

Published
2015
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