Search

The binding ability of human serum albumin (HSA) on active pharmaceutical ingredients (APIs) is one of the most important parameters in the early stages of drug discovery. In this study, an immobilized HSA-based tool was developed for the rapid and easy in vitro screening of API binding. The work explored the serious incompleteness in the identification of HSA used for in vitro screening published in the last five years. To mitigate this problem, a comprehensive analysis and immobilization studies were performed on the most used HSA types. Serious differences in the colloidal stability of HSAs and their API binding ability on a selected set of APIs were observed. HSAs were immobilized on magnetic nanoparticles with glutardialdehyde (GDA) or cyclohexyl-diglycidyl ether (CDGE) linkers, which have never been used for HSA immobilization before. The HSA-MNP-CDGE complexes achieved a higher immobilization yield and preserved API binding ability; however, the esterase-like enzymatic activity of HSA reduced significantly.

Background: Building extensive drug candidate libraries as early in the development pipeline as possible, with high-throughput in vitro absorption, distribution, metabolism, and excretion (ADME) profiling, is crucial for the selection of lead compounds to guide subsequent research and production phases. Traditionally, the analysis of metabolic stability assays heavily relies on high-throughput LC-MS/MS (liquid chromatography tandem mass spectrometry) techniques to meet with the lead profiling demands. Laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS) is a quick and efficient technique for characterizing complex biological samples without laborious sample preparation. Objective: In this study, using an automated LA-REIMS well plate reader, achieving an 8 s per sample measurement time, the oxidative metabolic stability of active drug agents was assessed using biomimetic metalloporphyrin-based oxidative model reactions. Results: The results obtained using the novel LA-REIMS-based protocol were compared to and corroborated by those obtained using conventional HPLC-UV-MS (high performance liquid chromatography with ultra-violet detection coupled with mass spectrometry) measurements. Conclusions: LA-REIMS emerges as a promising technique, demonstrating potential suitability for semi-quantitative high-throughput metabolic stability in an optimized solvent environment.

Human tyrosine hydroxylase (hTH) has key role in the production of catecholamine neurotransmitters. The structure, function and regulation of hTH has been extensively researched area and the possibility of enzyme replacement therapy (ERT) involving hTH through nanocarriers has been raised as well. However, our understanding on how hTH may interact with nanocarriers is still lacking. In this work, we attempted to investigate the immobilization of hTH on magnetic nanoparticles (MNPs) with various surface linkers in quantitative and mechanistic detail. Our results showed that the activity of hTH was retained after immobilization via secondary and covalent interactions as well. The colloidal stability of hTH could be also enhanced proved by Dynamic light scattering and Zeta potential analysis and a homogenous enzyme layer could be achieved, which was investigated by Raman mapping. The covalent attachment of hTH on MNPs via aldehyde or epoxy linkers provide irreversible immobilization and 38.1 % and 16.5 % recovery (ER). The hTH-MNPs catalyst had 25 % ER in average in simulated nasal electrolyte solution (SNES). This outcome highlights the relevance of immobilization applying MNPs as a potential formulation tool of sensitive therapeutic enzymes offering new opportunities for ERT related to neurodegenerative disorders., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)

The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications.

The investigation of liver-related metabolic stability of a drug candidate is a widely used key strategy in early-stage drug discovery. Metalloporphyrin-based biomimetic catalysts are good and well-described models of the function of CyP450 in hepatocytes. In this research, the immobilization of an iron porphyrin was performed on nanoporous silica particles via ionic interactions. The effect of the metalloporphyrin binding linkers was investigated on the catalytic efficiency and the metabolic profile of chloroquine as a model drug. The length of the amino-substituted linkers affects the chloroquine conversion as well as the ratio of human major and minor metabolites. While testing the immobilized catalysts in the continuous-flow reactor, results showed that the presented biomimetic system could be a promising alternative for the early-stage investigation of drug metabolites regarding analytical or synthetic goals as well.

Riverine floods cause increasingly severe damages to human settlements and infrastructure. Ecosystems have a natural capacity to decrease both severity and frequency of floods. Natural flood regulation processes along freshwaters can be attributed to two different mechanisms: flood prevention that takes place in the whole catchment and flood mitigation once the water has accumulated in the stream. These flood regulating mechanisms are not consistently recognized in major ecosystem service (ES) classifications. For a balanced landscape management, it is important to assess the ES flood regulation so that it can account for the different processes at the relevant sites. We reviewed literature, classified them according to these mechanisms, and analysed the influencing ecosystem characteristics. For prevention, vegetation biomass and forest extent were predominant, while for mitigation, the available space for water was decisive. We add some aspects on assessing flood regulation as ES, and suggest also to include flood hazard into calculations., (© 2022. The Author(s).)

Maintaining and, where possible, improving the ecological status of our water resources are of particular importance for the future. So, one of the main drivers of landscape design must be to protect our waters. In this study, we carried out an evaluation of four hydrologic ecosystem services (HES) in the Zala River catchment area, the largest tributary of Lake Balaton (more than half of the lake's surface inflow comes from the Zala River), Hungary. The lake has great ecological, economic and social importance to the country. We used the cell-based InVEST model to quantify the spatial distribution of flood control, erosion control and nutrient retention ecosystem services for phosphorus and nitrogen; then, we carried out an aggregated evaluation. Thereby, we localized the hot spots of service delivery and tested the effect of focused land use changes in critical areas of low performance on the examined four HES. Forests proved to have the best aggregated result, while croplands near the stream network performed poorly. The modelled change in land use resulted in significant improvement on nutrient filtration and moderate to minimal but improving change for the other HES in most cases. The applied method is suitable as a supporting tool at the watershed level for decision-makers and landscape designers with the aim of protecting water bodies., (© 2020. The Author(s).)

The dual functionalization of magnetic nanoparticles with inert (methyl) and reactive (aminopropyl) groups enables efficient immobilization of synthetic metalloporphyrins (such as 5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)iron(II) porphyrin and 5,10,15,20-tetrakis-(4-sulfonatophenyl)iron(II) porphyrin) via covalent or ionic interactions. The proportion of reactive function on the surface has significant effect on the biomimetic activity of metalloporphyrins. The optimized magnetic nanocatalyst containing porphyrin was successfully applied for biomimetic oxidation of antihypertensive drug Amlodipine in batch and continuous-flow reactors as well.

Groundwater (GW) in many regions is essential for agricultural productivity, especially during drought periods. The shrinking of GW is an important but rarely documented component of the recent global environmental crisis and may threaten food security. The problem cannot be put in proper perspective, because we rarely have datasets long and detailed enough to scrutinise the unfolding effects at regional scales. To address this knowledge gap, we used a 50-y long (1961-2010) and spatially extensive (283 GW wells) dataset from Hungary to examine the GW trends and the sensitivity of the yields of two important crops to GW fluctuations. During 1986-2010, GW levels were significantly (0.21-0.60 m) lower than during 1961-1985 in every region of Hungary and every month of the year. The decrease was 2.24 cm y -1 at the country level. Linear and bootstrap resampling tests indicated weak relationship between GW levels and wheat yields but decreasing GW levels accounted for 18-38% of maize yield variability during the 'climate change affected' period of 1986-2010. Calculating the impact of GW on potential food production, a 100 mm higher GW levels would have increased annual maize yields by 0.23 t ha -1 on the Hungarian Plain. However, the registered GW decrease caused an estimated maize yield loss of 0.65 t ha -1 , i.e. 11.6% of the average annual yield during 1986-2010. GW level fluctuations on the plain showed a significant correlation with August-October soil moisture gridded data over much of the agricultural landscapes of Central and Western Europe, indicating a similar situation in a wider European context. To mitigate the cumulative negative impact of GW decrease and the rising temperature, GW recharge via infiltration of retained water would be an adequate solution. Areas of former floodplains with low agroecological suitability, amounting to almost a quarter of the Hungarian Plain could serve as such water retention areas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)

Biomimetic oxidation of drugs catalyzed by metalloporphyrins can be a novel and promising way for the effective and sustainable synthesis of drug metabolites. The immobilization of 5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)iron(II) porphyrin (FeTPFP) and 5,10,15,20-tetrakis-(4-sulfonatophenyl)iron(II) porphyrin (FeTSPP) via stable covalent or rapid ionic binding on aminopropyl-functionalized magnetic nanoparticles (MNPs-NH 2 ) were developed. These immobilized catalysts could be efficiently applied for the synthesis of new pharmaceutically active derivatives and liver related phase I oxidative major metabolite of an antiarrhythmic drug, amiodarone integrated in a continuous-flow magnetic chip reactor (Magnechip)., Competing Interests: The authors declare no conflict of interest.

A medium-throughput screening (MTS) of biomimetic drug metabolite synthesis is developed by using an iron porphyrin catalyst. The microplate method, in combination with HPLC-MS analysis, was shown to be a useful tool for process development and parameter optimization in the production of targeted metabolites and/or oxidation products of forty-three different drug substances. In the case of the biomimetic oxidation of amiodarone, the high quantity and purity of the isolated products enabled detailed HRMS and NMR spectroscopic studies. In addition to identification of known metabolites, several new oxidation products of the drug that was studied were characterized. Fast degradation and poor recovery of the catalyst under batch conditions was overcome by immobilization of 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin iron(III) chloride (FeTSPP) on the surface of 3-aminopropyl-functionalized silica by electrostatic interaction. The supported catalyst was successfully applied in a packed-bed reactor under continuous-flow reaction conditions for the large-scale synthesis of amiodarone metabolites., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Abstract: The impacts of global warming are the subject of intense research. However, it is difficult to agree on ways to prevent and mitigate adverse changes until the root causes of the problem can be formulated in simple and understandable terms. The aim of this study is to highlight changes in the hydrological cycle using a new graphical method, the Climatic Energy Balance Diagram (CEBD), or climate diagram for short. The key terms of the energy balance equation determine the annual operating points or working points of the climatic energy distribution process. The easy-to-use visualization tool promotes evaporative fraction (EF) as a key metrics, expressing the efficiency of the climatic energy distribution process. Analysis has shown that keeping it at around 0.7 in the Danube basin requires increased evaporation. The 30-year average value of evaporation has increased by 29 mm (from 482 mm to 507 mm) while that of sensible heat by 23 mm (from 196 mm to 219 mm) between 1961–1990 and 1991–2020, both expressed in water-depth equivalents. A normalised version of the CEBD can be used to compare areas in different climatic conditions. The diagram highlights the key role of water and evaporation in the process of distributing climatic energy to ensure environmental sustainability and can facilitate better communications between different research areas and stakeholders (farmers, regulators, investors, policy makers) while maintaining a multidisciplinary approach. [ABSTRACT FROM AUTHOR]

This study investigates the immobilization of Lactobacillus paracasei AAL (LpAAL) protein onto polyvinyl alcohol/nylon 6/chitosan nanofiber membranes using dextran polyaldehyde as a biodegradable cross-linker. Immobilization enhanced the enzyme's stability, shifting its optimal reaction conditions from 40 °C to 45 °C and pH from 8.0 to 8.5. While immobilization slightly reduced its catalytic efficiency, it significantly improved enzyme stability and reusability. The immobilized enzyme retained 85% of its initial activity after 7 days of storage at room temperature, compared to 55% for the free enzyme. Reusability tests demonstrated that immobilized LpAAL protein maintained approximately 50% of its activity after six consecutive reaction cycles, highlighting its robustness over repeated use. These results underscore the advantages of nanofiber-based immobilization in enhancing enzyme stability and utility for industrial applications, offering a practical approach to overcoming the limitations associated with free enzyme systems. [ABSTRACT FROM AUTHOR]

Assessments of the agro-climatic resources of Northern Kazakhstan are urgently needed in the face of climate change and increasing threats to food security in the world, and they can provide valuable information for specialists in the field of agriculture. To assess the agro-climatic conditions of Northern Kazakhstan, the following agro-climatic indices were used: heat availability, moisture availability, and aridity of the growing season for the period 1991–2023. The research results rendered it possible to build maps of the spatial distribution of agro-climatic indicators, and five agro-climatic zones, ranging from "moderately humid moderately warm" in the north to "very arid moderately hot" in the south of Northern Kazakhstan, were identified. Recommendations were developed with respect to the agro-climatic zoning of main crops, taking into account the climatic resources of Northern Kazakhstan. The data obtained will be used for the strategic planning of the agricultural crop industry in Northern Kazakhstan. [ABSTRACT FROM AUTHOR]

This study investigated the implementation of agroecological principles on three organic farms in Hungary, focusing on four resource-focused, nature conservation-related agroecological basic elements identified by the FAO: biodiversity, interactions, recycling, and resilience. This research employed a mixed-methods approach, utilising in-depth interviews as a technique to explore farmers' practices and a questionnaire survey as a tool to assess consumer perspectives (with 63 respondents). The interviews facilitated a qualitative exploration of how agroecological practices are applied on farms, providing rich insights into the farmers' experiences. Meanwhile, the questionnaire survey served as a structured instrument to measure consumer awareness and motivations concerning environmentally friendly farming methods. NVivo 12 software was employed for qualitative data analysis, assisting in coding and organizing responses to better understand recurring themes and patterns. The researchers found that all farms exhibited high biodiversity levels, facilitated through practices such as companion planting, crop rotation, and maintaining natural habitat patches. Agroecological farmers focus on practices suited to the landscape, fostering beneficial organisms and enhancing interactions between nature and agriculture. Integrating farm components (e.g., crops, livestock, water) promotes synergies that improve productivity and reduce reliance on external inputs. Recycling resources (like organic waste) within the farm increases efficiency, while resilience is strengthened through biodiversity, allowing farms to better withstand environmental stress. Direct marketing builds connections between producers and consumers, raising awareness of conservation practices. Consumer awareness regarding environmentally friendly agricultural practices was notably high, with findings indicating that health and ecological conservation motivations drive their purchasing decisions. This study highlights the context-dependent nature of agroecological practices, revealing that while implementation is robust, economic sustainability constraints may limit the extent to which all elements can be effectively applied. Since this research has certain limitations due to the limited sample size, expanding the study to include more farms would strengthen the findings. Nonetheless, these findings underscore the importance of integrating agroecological principles in organic farming to enhance biodiversity and foster sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Accurately delineating sediment export dynamics using high-quality vegetation factors remains challenging due to the spatio-temporal resolution imbalance of single remote sensing data and persistent cloud contamination. To address these challenges, this study proposed a new framework for estimating and analyzing monthly sediment inflow to rivers in the cloud-prone Minjiang River Basin. We leveraged multi-source remote sensing data and the Continuous Change Detection and Classification model to reconstruct monthly vegetation factors at 30 m resolution. Then, we integrated the Chinese Soil Loss Equation model and the Sediment Delivery Ratio module to estimate monthly sediment inflow to rivers. Lastly, the Optimal Parameters-based Geographical Detector model was harnessed to identify factors affecting sediment export. The results indicated that: (1) The simulated sediment transport modulus showed a strong Coefficient of Determination (R2 = 0.73) and a satisfactory Nash–Sutcliffe Efficiency coefficient (0.53) compared to observed values. (2) The annual sediment inflow to rivers exhibited a spatial distribution characterized by lower levels in the west and higher in the east. The monthly average sediment value from 2016 to 2021 was notably high from March to July, while relatively low from October to January. (3) Erosive rainfall was a decisive factor contributing to increased sediment entering the rivers. Vegetation factors, manifested via the quantity (Fractional Vegetation Cover) and quality (Leaf Area Index and Net Primary Productivity) of vegetation, exert a pivotal influence on diminishing sediment export. [ABSTRACT FROM AUTHOR]

Astragalus is the vegetation of many mountains of Iran's plateau and plays a major role in providing ecosystem services due to its pillow shape and deep rooting system, they facilitate the control and penetration of precipitation into the soil. The correlation of Astragalus ecosystems with arid and semi-arid climates has made them vulnerable to climate change. In this study, a runoff yield map based on the Budyco curve under current and future conditions of climate change (2050) was prepared using climate and temperature data from the Chelsea site (CanESM2 GCM) in TerrSet software and by using maps of sub-watersheds, annual precipitation, annual potential evapotranspiration, soil depth, plant accessible water and the current and future "Land Cover - Land Use" map, with a combination of field methods and species distribution models at the local scale of the Shur River watershed of Dehaghan (Central Zagros). Finally, the excess runoff damage produced due to climate change was estimated using the replacement cost method. The results indicated an increase in the annual runoff volume of the watershed from 70 million cubic meters to 105 million cubic meters under climate change conditions for the RCP26 scenario in 2050. Taking into account the cost of 10 million Rials for controlling 530 cubic meters of runoff through various watershed management projects, preventing the damages of excess runoff produced requires a credit amounting to 660 billion Rials based on the present value. This study proved the ability of TerrSet software to predict and produce an ecosystem service map of runoff yield under climate changes or land use changes and with the purpose of valuation on a local scale. Also, the above valuation can be the basis for planning and providing credit for the study and implementation of watershed management projects to deal with the threats of climate change. [ABSTRACT FROM AUTHOR]

Copyright of Environmental & Social Management Journal / Revista de Gestão Social e Ambiental is the property of Environmental & Social Management Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Hydrological Ecosystem Services (HES) are crucial components of environmental sustainability and provide indispensable benefits. The present study identifies critical hot and cold spots areas of HES in the Aglar watershed of the Indian Himalayan Region using six HES descriptors, namely water yield (WYLD), crop yield factor (CYF), sediment yield (SYLD), base flow (LATQ), surface runoff (SURFQ), and total water retention (TWR). The analysis was conducted using weightage-based approaches under two methods: (1) evaluating six HES descriptors individually and (2) grouping them into broad ecosystem service categories. Furthermore, the study assessed pixel-level uncertainties that arose because of the distinctive methods used in the identification of hot and cold spots. The associated synergies and trade-offs among HES descriptors were examined too. From method 1, 0.26% area of the watershed was classified as cold spots and 3.18% as hot spots, whereas method 2 classified 2.42% area as cold spots and 2.36% as hot spots. Pixel-level uncertainties showed that 0.57 km2 and 6.86 km2 of the watershed were consistently under cold and hot spots, respectively, using method 1, whereas method 2 identified 2.30 km2 and 6.97 km2 as cold spots and hot spots, respectively. The spatial analysis of hot spots showed consistent patterns in certain parts of the watershed, primarily in the south to southwest region, while cold spots were mainly found on the eastern side. Upon analyzing HES descriptors within broad ecosystem service categories, hot spots were mainly in the southern part, and cold spots were scattered throughout the watershed, especially in agricultural and scrubland areas. The significant synergistic relation between LATQ and WYLD, and sediment retention and WYLD and trade-offs between SURFQ and HES descriptors like WYLD, LATQ, sediment retention, and TWR was attributed to varying factors such as land use and topography impacting the water balance components in the watershed. The findings underscore the critical need for targeted conservation efforts to maintain the ecologically sensitive regions at watershed scale. [ABSTRACT FROM AUTHOR]

Climatic conditions are the main factor influencing the suitability of agricultural land for crop production. Therefore, the evaluation of climate change impact on crop suitability using the best possible methods and data is needed for successful agricultural climate change adaptation. This study presents the application of a multi-criteria evaluation approach to assess climate suitability for maize production in Poland, for a baseline period (BL, 1981–2010) and two future periods 2041–2070 (2050s) and 2071–2100 (2080s) under two RCP (Representative Concentration Pathways) scenarios: RCP4.5 and RCP8.5. The analyses incorporated expert knowledge using the Analytical Hierarchy Process (AHP) into the evaluation of criteria weights. The results showed that maturity and frost stress were the most limiting factors in assessing the climatic suitability of maize cultivation in Poland, with 30% and 11% of Poland classified as marginally suitable or not suitable for maize cultivation, respectively. In the future climate, the area limited by maturity and frost stress factors is projected to decrease, while the area of water stress and heat stress is projected to increase. For 2050 climate projections, water stress limitation areas occupy 7% and 8% of Poland for RCP4.5 and RCP8.5, respectively, while for 2080 projections, the same areas occupy 12% and 32% of the country, respectively. By 2080, heat stress will become a limiting factor for maize cultivation; according to our analysis, 3% of the Polish area under RCP8.5 will be marginally suitable for maize cultivation because of heat stress. The overall analyses showed that most of Poland in the BL climate is in the high suitability class (62%) and 38% is moderately suitable for maize cultivation. This situation will improves until 2050, but will worsen in the 2080s under the RCP8.5 scenario. Under RCP8.5, by the end of the century (2080s), the highly suitable area will decrease to 47% and the moderately suitable area will increase to 53%. [ABSTRACT FROM AUTHOR]

The Czech Republic has diverse soil conditions, which cause notable differences in crop water balance (WB). To assess the long-term crop WB and crop water stress (CWS) intensity in rainfed conditions, four conventional eight- and ten-year crop rotations (CRs) with perennial forage (alfalfa), cereals, oilseeds, root crops and legumes were proposed for a heterogeneous lowland soil region (six texture classes) in eastern Bohemia. Two of the CRs were selected irrespective of the WB (eight-year, C-8; ten-year, C-10), and the other two were designated according to soil water resources and crop water requirements (CWRs) as water-saving (W-S) and water-demanding (W-D) for this region. All CRs had a negative WB on average (i.e., CWRs exceeded the available water resources), with varying degrees of CWS (categories 1 (low) to 4 (high)). The W-S CR reduced the WB deficit relative to the other CRs by omitting maize, sunflower and sugar beet and including sorghum, and expanded the proportion of the area not affected by CWS (categories 1–2) to 33% for predominantly loamy soils. In contrast, categories 1–2 in the C-8, C-10 and W-D CRs represented only 15%, 14% and 7% of the area, respectively. Other areas were significantly affected by CWS (categories 3–4) and showed a high risk of yield reduction. These results may help in implementing sustainable farming systems that consider environmental perspectives related to agricultural water use in Central Europe. [ABSTRACT FROM AUTHOR]

Spring wheat (Triticum aestivum L.) remains an important alternative to winter wheat cultivation at Northern latitudes due to high risk of overwintering or delayed sowing of winter wheat. We studied nine major agronomic traits in a set of 299 spring wheat genotypes in trials across 12-year-site combinations in Lithuania, Latvia, Estonia, and Norway for three consecutive years. The dataset analyzed here consisted of previously published phenotypic data collected in 2021 and 2022, supplemented with additional phenotypic data from the 2023 field season collected in this study. We combined these phenotypic datasets with previously published genotypic data generated using a 25K single nucleotide polymorphism (SNP) array that yielded 18,467 markers with a minor allele frequency above 0.05. Analysis of these datasets via genome-wide association study revealed 18 consistent quantitative trait loci (QTL) replicated in two or more trials that explained more than 5% of phenotypic variance for plant height, grain protein content, thousand kernel weight, or heading date. The most consistent markers across the tested environments were detected for plant height, thousand kernel weight, and days to heading in eight, five, and six trials, respectively. No beneficial effect of the semi-dwarfing alleles Rht-B1b and Rht-D1b on grain yield performance was observed across the 12 tested trials. Moreover, the cultivars carrying these alleles were low yielding in general. Based on principal component analysis, wheat genotypes developed in the Northern European region clustered separately from those developed at the southern latitudes, and markers associated with the clustering were identified. Important phenotypic traits, such as grain yield, days to heading, grain protein content, and thousand kernel weight were associated with this clustering of the genotype sets. Interestingly, despite being adapted to the Nordic environment, genotypes in the Northern set demonstrated lower grain yield performance across all tested environments. The results indicate that spring wheat germplasm harbors valuable QTL/alleles, and the identified traitmarker associations might be useful in improving Nordic-Baltic spring wheat germplasm under global warming conditions. [ABSTRACT FROM AUTHOR]

Both globally and in Hungary, agriculture is one of the industries that is most vulnerable to weather and climate extremes. Intense temperature rises, spatial and temporal variations in precipitation, and significant changes in extreme climatological and weather parameters have contributed to changes in the conditions of cropland, crop losses, and impacts on crop quality in recent years. This paper depicts the transformation of the domestic agricultural sector due to the extreme drought shock of 2022, as well exploring the adaptation strategies applied. The research is based on official agro-climate database and crop data, and the temperature, precipitation, and radiation during the growing season are all examined. The agro-meteorological properties in Hungary had to be investigated for the entire year and all four of its seasons, with indicator analysis projected onto the ever-increasing and dormant seasons. Long-term climate analysis is necessary to understand the historic drought of 2022 and the success of future adaptation and mitigation techniques. The results can help smallholders effectively reduce the adverse impacts of drought conditions, thereby increasing their adaptation to similar shocks. [ABSTRACT FROM AUTHOR]

The digitization of agriculture is widely discussed today. But despite proven benefits, its acceptance in agricultural practice remains low. In small-structured areas, this trend is even more pronounced. There are even known cases where farmers initially purchased and used technology, but then stopped using it due to lack of profitability or other reasons. Interestingly, despite extensive research on precision agriculture technologies (PATs), the processes of adoption and phase-out with their associated economic impacts have never been studied. This paper provides a methodological framework for evaluating the economics of PAT deployment, taking into account changes during the period of use; the framework provides decision rules for determining the appropriate time to phase out technology. Using a selected PAT, a farm model, and defined entry and exit scenarios, it was shown that farms with outdated technology and farms with retrofittable technology are at a significant economic disadvantage during implementation compared to farms already using technology suitable for site-specific fertilization or farms relying on the use of a contractor. And even in the event of a phase-out, the two disadvantaged starting conditions face significantly greater uncertainties and costs. Moreover, the decision to phase out in time is difficult, as making an informed and fact-based decision is not possible after the first year of use. Therefore, it is advisable that farmers are not only accompanied before and during phase-in, but also receive professional support during use. [ABSTRACT FROM AUTHOR]

Climate extremes are on the rise. Impacts of extreme climate and weather events on ecosystem services and ultimately human well‐being can be partially attenuated by the organismic, structural, and functional diversity of the affected land surface. However, the ongoing transformation of terrestrial ecosystems through intensified exploitation and management may put this buffering capacity at risk. Here, we summarize the evidence that reductions in biodiversity can destabilize the functioning of ecosystems facing climate extremes. We then explore if impaired ecosystem functioning could, in turn, exacerbate climate extremes. We argue that only a comprehensive approach, incorporating both ecological and hydrometeorological perspectives, enables us to understand and predict the entire feedback system between altered biodiversity and climate extremes. This ambition, however, requires a reformulation of current research priorities to emphasize the bidirectional effects that link ecology and atmospheric processes. Plain Language Summary: Climate extremes are increasing and impacting both nature and people. We hypothesize that intact ecosystems, particularly via their biodiversity, can mitigate the impacts of climate extremes. What happens when biodiversity decreases? Could this loss make the effects of climate extremes even worse or change how these events occur? We explore these two questions and summarize the current state of knowledge. We conclude that targeted research efforts at the interface of ecology and atmospheric sciences are needed to answer these questions conclusively. Key Points: Mounting evidence suggests that an ecosystem's capacity to buffer the impacts of climate extremes depends on its biodiversityNumerous mechanisms suggest that a reduction in biodiversity could exacerbate climate extremesA series of research gaps need to be addressed to understand the full feedback between biodiversity change and climate extremes [ABSTRACT FROM AUTHOR]

Land use and land cover (LULC) changes alter the structure and functioning of natural ecosystems, impacting the potential and flow of ecosystem services. Ecological restoration projects aiming to enhance native vegetation have proven effective in mitigating the impacts of LULC changes on ecosystem services. A key element in implementing these projects has been identifying priority areas for restoration, considering that resources allocated to such projects are often limited. This study proposes a novel methodological framework to identify priority areas for restoration and guide LULC planning to increase the provision of water ecosystem services (WESs) in a watershed in southeastern Brazil. To do so, we combined biophysical models and multicriteria analysis to identify priority areas for ecological restoration, propose environmental zoning for the study area, and quantify the effects of LULC changes and of a planned LULC scenario (implemented environmental zoning) on WES indicators. Previous LULC changes, from 1985 to 2019, have resulted in a nearly 20% increase in annual surface runoff, a 50% increase in sediment export, a 22% increase in total nitrogen (TN) export, and a 53% increase in total phosphorus (TP) export. Simultaneously, they reduced the provision of WESs (baseflow −27%, TN retention −10%, and TP retention −16%), except for sediment retention, which increased by 35% during the analyzed period. The planned LULC scenario successfully increased the provision of WESs while reducing surface runoff and nutrient and sediment exports. The methodology employed in this study proved to be effective in guiding LULC planning for improving WES. The obtained results provide a scientific foundation for guiding the implementation of WES conservation policies in the studied watershed. This method is perceived to be applicable to other watersheds. [ABSTRACT FROM AUTHOR]

The study of lubricating oil is paramount for the optimal functioning of modern engines, and it has generated intensive research in the automotive industry. The aim is to improve the tribological properties of lubricants by including nanomaterials as additives in base oils. This article presents an exhaustive bibliographic review of the experiments carried out to optimize the tribological properties of nano-lubricants in order to identify the nanoparticles and experimental processes used and analyze the results obtained. The methodology adopted combines inductive and deductive elements. It begins with the formulation of a general theory on the application of nanoparticles in lubricants, followed by the collection of specific data on the conceptualization and preparation of nano-lubricants. A total of 176 articles focused on the application of nanoparticles in lubricants, especially to reduce the coefficient of friction, are reviewed. These works, with impact levels Q1 and Q2, delve into the application and are analyzed to review the obtained results. Most researchers worked with a nanoparticle concentration range of 0% to 1% by volume. [ABSTRACT FROM AUTHOR]

The uncertainty in soil hydraulic parameters is often not taken into account in process‐based hydrological modeling. Performing runs with 104 stochastic parameter realizations, we evaluated the propagation of uncertainty in the Van Genuchten–Mualem (VGM) parameters into estimates of the threshold values of soil water content used to calculate the total and readily available water, and on the long‐term (30 years) simulations of evaporation, transpiration, bottom flux, and runoff by the SWAP hydrological model. The simulated scenarios included weather data from a location in southeast Brazil and seven soils from the same region cropped with maize, comprising a wide range of texture classes. The results showed that uncertainties in VGM parameters affect the estimates of total and readily available water. Water balance components obtained by a deterministic simulation with average VGM parameters did not always agree with the average or median of stochastic simulations, and stochastic simulations including parameter uncertainties should be preferred. Variations in yearly rainfall characteristics were more important for bottom flux and evaporation, while transpiration and runoff were more strongly influenced by the variations in soil hydraulic properties. Core Ideas: Hydraulic parameter uncertainty and correlations can be used in stochastic simulations with a hydrological model.Parameter uncertainty propagates more strongly into estimates of plant available water than in its threshold water contents.Stochastic averages of simulated water balance components may differ from deterministically obtained values.Bottom flux and evaporation respond to rainfall, while transpiration and runoff depend most on soil properties. [ABSTRACT FROM AUTHOR]

Copyright of Gazdálkodás is the property of Herman Otto Intezet Nonprofit Kft. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The global escalation in cereal production, essential to meet growing population demands, simultaneously augments the generation of cereal crop residues, estimated annually at approximately 3107 × 106 Mg/year. Among different crop residue management approaches, returning them to the soil can be essential for various ecological benefits, including nutrient recycling and soil carbon sequestration. However, the recalcitrant characteristics of cereal crop residues pose significant challenges in their management, particularly in the decomposition rate. Therefore, in this review, we aim to summarize the influence of different agricultural practices on enhancing soil microbial decomposer communities, thereby effectively managing cereal crop residues. Moreover, this manuscript provides indirect estimates of cereal crop residue production in Northern Europe and Lithuania, and highlights the diverse roles of lignocellulolytic microorganisms in the decomposition process, with a particular focus on enzymatic activities. This review bridges the knowledge gap and indicates future research directions concerning the influence of agricultural practices on cereal crop residue-associated microbial consortia. [ABSTRACT FROM AUTHOR]

Central and Eastern Europe (CEE) is a pillar of global wheat and maize production. However, certain areas within the CEE region have become climate change hotspots, experiencing intensifying water deficits and drought pressure, rising mean and maximum temperatures. This study focuses on the long-term statistical relationships between climatic factors and rain-fed wheat and maize yields for different landscape types in Hungary over 30-year time windows between 1921 and 2010. The relationship between the variances of the detrended climatic parameters and crop yields was tested employing both simple and multifactorial linear models according to landscape types and periods. The sensitivity of wheat yields to spring–summer mean temperature shifted dynamically from the western part of the country to east (from cooler and wetter hilly landscapes to plains) between the periods 1921–1950 and 1981–2010. The cooling observed in summer temperature between the periods 1921–1950 and 1951–1980 supported an increase in wheat yields by an estimated 0.11–0.43 t ha−1 year−1, while the 0.9–1.2 °C warming of May–July temperature may have cut wheat yields by an estimated 0.44–0.56 t ha−1 year−1 in various regions over 1981–2010. That being said, the regional sensitivity of wheat yields to May–July mean temperature did not display substantial differences between the periods 1921–1950 and 1981–2010. Besides negative effects, climate change had a positive impact on wheat yields, since increasing January–March mean temperatures mitigated the negative impact of warming summer temperatures on wheat yields by an estimated 16–34% over 1981–2010. In this 30-year period, increasing mean temperature together with decreasing precipitation explained 46–75% of the variances in maize yields reducing annual maize harvests by an estimated 11.1–12.4% year−1. [ABSTRACT FROM AUTHOR]

The 2022 drought highlighted Hungary's vulnerability to climate change, especially the Great Hungarian Plain. Soil moisture, which is crucial for agriculture, depends on the position of the shallow groundwater table. This study investigated the effects of climate change on groundwater table fluctuations in more than 500 wells on the plain. An integrated vertical hydrological model, assuming negligible horizontal subsurface flows, employed the Dunay–Varga-Haszonits methodology for evaporation and Kovács approach for the water retention curve. Verified with two meteorological databases, the model was accepted for 463 wells based on NSE > 0.4 and RMSE < 0.5 m criteria. The FORESEE HUN v1.0 dataset proved suitable after spatial consistency tests. Examining 28 bias- and discontinuity-corrected climate model projections on these wells revealed a general decline in the groundwater table. Differences between trends to 2050 and 2100 suggested lower groundwater levels by mid-century. This research highlights climate change impacts in a crucial Central-European agricultural region in the Carpathian Basin and emphasizes the importance of modeling climate change-induced changes in shallow groundwater levels in water resources management. [ABSTRACT FROM AUTHOR]

The extreme drought in Europe in 2022 also hit hard the Great Hungarian Plain. In this short overview article, we summarize the natural environmental conditions of the region and the impact of river control works on the water-retention capacity of the landscape. In this respect, we also review the impact of intensive agricultural cultivation on soil structure and on soil moisture in light of the meteorological elements of the 2022 drought. The most important change is that the soil stores much less moisture than in the natural state; therefore, under the meteorological conditions of summer 2022, the evapotranspiration capacity was reduced. As a result, the low humidity in the air layers above the ground is not sufficient to trigger summer showers and thunderstorms associated with weather fronts and local heat convection anymore. Our proposed solution is to restore about one-fifth of the area to the original land types and usage before large-field agriculture. Low-lying areas should be transformed into a mosaic-like landscape with good water supply and evapotranspiration capacity to humidify the lower air layers. Furthermore, the unfavorable soil structure that has resulted from intensive agriculture should also be converted into more permeable soil to enhance infiltration. [ABSTRACT FROM AUTHOR]

Due to unsustainable land management and climate change, floods have become more frequent and severe over the past few decades and the problem is exacerbated in urban environments. In the context of climate-proofing cities, the importance of nature-based solutions (NBSs), obtaining relevant outcomes in the form of ecosystem services, has been highlighted. Although the role of ecosystem services in building resilience against negative climate change effects is widely recognized and there is an identified need to better integrate ecosystem services into urban planning and design, this has proven difficult to operationalize. A critical limitation is that modeling is a time-consuming and costly exercise. The purpose is to roughly estimate the ecosystem service of water run-off mitigation through simplified, cost-effective, and user-friendly modelling at three nested biophysical scales, under four climate change scenarios. Using the Swedish city of Gothenburg as an example, we propose an approach for navigating NBS-oriented flooding adaptation strategies, by quantifying the ecosystem service of water run-off mitigation at three nested biophysical scales, under four climate change scenarios, hence, proposing an approach for how to navigate nature-based solutions in a multi-scale, social–ecological urban planning context against present and future flooding events. Our findings validate the effectiveness of employing an ecosystem service approach to better comprehend the significant climate change issue of flooding through user-friendly and cost-efficient modeling. [ABSTRACT FROM AUTHOR]

The Yangtze River Basin (YZRB) and the Yellow River Basin (YRB), which are crucial for ecology and economy in China, face growing challenges to ecosystem service (ES) functions due to global population growth, urbanization, and climate change. This study assessed the spatiotemporal dynamics of ESs in the YZRB and the YRB between 2001 and 2021, comprehensively encompassing essential aspects such as water yield (WY), carbon sequestration (CS), soil conservation (SC), and habitat quality (HQ) while also analyzing the trade-offs and synergies among these ESs at the grid cells. The GeoDetector was employed to ascertain individual or interactive effects of natural and anthropogenic factors on these ESs and their trade-offs/synergies. The results showed that (1) from 2001 to 2021, the four ESs exhibited significant spatial disparities in the distribution within two basins, with the overall trend of ESs mainly increasing. YZRB consistently exhibited substantially higher ES values than the YRB. (2) Complex trade-offs and synergies among these ESs were apparent in both basins, characterized by distinct spatial heterogeneity. The spatial relationships of WY–CS, WY–SC, CS–SC, and CS–HQ were mainly synergistic. (3) Precipitation, potential evapotranspiration, elevation, land use and land cover (LULC), and slope influenced ESs in both basins. Notably, interactive factors, particularly the interactions involving LULC and other factors, demonstrated more robust explanatory power for ESs and their trade-offs/synergies than individual drivers. These findings significantly affect the refined ecosystem management and sustainable development decision-making in large rivers or regions. [ABSTRACT FROM AUTHOR]

This document is a summary of a special issue of the journal Sustainability titled "Assessment of Ecosystem Services at Different Scales." The issue includes nine articles that focus on the assessment of ecosystem services (ESs) using various methodologies at different spatial scales. The articles cover topics such as the assessment of ESs at national and regional scales, the use of economic valuation methods, participatory mapping of ESs, and the integration of ESs in environmental impact assessments. The papers highlight the importance of ES assessment for informing decision-making and advancing sustainable land use and habitat conservation. The authors acknowledge the challenges and limitations of ES assessments but emphasize the value of using primary data, secondary data, and expert judgments to inform policy-making. [Extracted from the article]

Purpose: According to standard procedure recommended by the Water Framework Directive (WFD), dissolved concentrations of potentially toxic elements (PTEs) in river water are determined by inductively coupled plasma mass spectrometry (ICP-MS) in filtered (0.45 µm) and acidified (pH 2) samples. Properly prepared and stored composite samples can enhance the temporal representativity of monitoring without increasing analytical costs. For this purpose, the WFD recommends freezing, which can preserve the species integrity and prevent adsorption processes of PTEs. Methods: Low storage temperature in hard water samples can trigger precipitation of calcium carbonate (CaCO3) and subsequent co-precipitation of PTEs. To test and determine to what extent co-precipitation with CaCO3 can influence the determination of PTE concentrations, composite river water samples from two case study catchments in Hungary (Zagyva and Koppány) were prepared following two different sample preservation procedures. To study the behavior of PTEs in river water during storage, in the first procedure, samples were frozen, and they were thawed, filtered, and acidified directly prior to the analysis. In the second procedure, samples were filtered on-site and acidified prior to freezing to prevent precipitation of CaCO3 and then only thawed to carry out the chemical analyses. Concentrations of PTEs were determined by ICP-MS. Results: A statistical evaluation of the results using Student's t-test revealed significant differences between the two sample preservation procedures, suggesting that PTEs were largely co-precipitated with CaCO3 if the samples were not acidified prior to freezing. Conclusion: When establishing protocols for sample preservation procedures, the phenomenon of co-precipitation of PTEs with CaCO3 should be considered if the samples were not acidified before freezing. Therefore, to prevent co-precipitation of PTEs with CaCO3, samples should be filtered and acidified before freezing. [ABSTRACT FROM AUTHOR]

Changes in land cover affect ecosystems and the services they provide. The aim of this study was to assess the spatial changes in land use from 1990 to 2018 and analyze the changes in ecosystem service value (ESV) in response to the changes in landscape structure in Lithuania. Croplands provided the majority of the ESV, followed by forests, grasslands, and wetlands. The total ESV in Lithuania was USD 29 billion year−1 in 2018, and land use had a significant impact on ESV, with a total decrease of USD 438 million year−1 between 1990 and 2018. The total ESV change was mainly influenced by the decrease in provisioning (USD 426 million year−1) and regulating (USD 208 million year−1) services. The reduction in cropland area was a major factor in the loss of ESV. The increase in the value of habitat and cultural services was not large enough to offset the reduction of the ESV. The highest elasticities were in the urban municipalities, indicating a significant change in ESV due to land use change. The results of this study provide valuable insight into the potential of the sustainable management and regeneration of ecosystems. [ABSTRACT FROM AUTHOR]

The large-scale loss of ecosystem assets around the world, and the resultant reduction in the provision of nature's benefits to people, underscores the urgent need for better metrics of ecological performance as well as their integration into decision-making. Gross ecosystem product (GEP) is a measure of the aggregate monetary value of final ecosystem-related goods and services in a specific area and for a given accounting period. GEP accounting captures the use of many ecosystem services in production processes across the economy, which are then valued in terms of their benefits to society. GEP has five key elements that make it transparent, trackable, and readily understandable: (1) a focus on nature's contributions to people; (2) the measurement of ecosystem assets as stocks and ecosystem services as flows; (3) the quantification of ecosystem service use; (4) an understanding of ecosystem service supply chains through value realization; and (5) the disaggregation of benefits across groups. Correspondingly, a series of innovative policies based on GEP have been designed and implemented in China. The theoretical and practical lessons provided by these experiences can support continued policy innovation for green and inclusive development around the world. [ABSTRACT FROM AUTHOR]

Floodplains are important ecosystems that contribute to the ecological stability of the landscape. A number of ecosystem functions and services are significantly influenced by ecological aspects of floodplain habitats. This article focuses on the ecological quality and estimated amount of carbon stored in the biomass of habitats located in the studied watersheds, with an emphasis on floodplains. The habitats and their ecological quality were determined and assessed using the Biotope Valuation Method (BVM), an expert method for evaluating habitat (biotope) types based on eight ecological characteristics, mainly concerning various aspects of their biodiversity and vulnerability. The objective of this study is to compare the resulting assessments of habitats located in floodplains with assessments of habitats situated in the surrounding landscape. The study was carried out on three selected small stream watersheds in the South Moravian Region of the Czech Republic, which differ from each other in terms of the predominant land use and the overall level of anthropogenic pressure on the landscape. The results indicate that floodplains have a higher ecological value compared to the surrounding landscape, except for floodplains in areas with intensive agriculture. The ability of floodplains to store carbon in biomass turned out to be higher in the watershed with a higher percentage of tree stands, where woody plants store significantly more carbon in the biomass compared to other types of vegetation. It has been shown that human pressure on floodplains and land use significantly affects ecosystem functions and services. In addition to the intensity of agriculture, these were, in particular, pressures from an expansion of built-up areas and infrastructure developments, and forest management. In this study, forest stands in floodplain were more stable and had a more beneficial species composition than forests in the surrounding landscape. [ABSTRACT FROM AUTHOR]

In recent years, a large-scale afforestation campaign has been implemented in Inner Mongolia, China, to control desertification and soil erosion. However, the water consumption associated with large-scale afforestation significantly impacts the water resources in Inner Mongolia, resulting in a major ecological risk. This study aimed to evaluate the ecological risk of water resources caused by afforestation in the region. In this study, using land cover data, normalized difference vegetation index (NDVI) data, and meteorological data, we performed trend analysis and used the water balance equation and water security index (WSI) to analyze the ecological risks of water resources caused by afforestation in Inner Mongolia from 2000 to 2020. The results show that (1) the afforestation area in Inner Mongolia was 5.37 × 104 km2 in 2000–2020; (2) afforestation in arid and semi-arid areas led to a reduction in water resources; (3) afforestation reduced water resources in the study area by 62 million cubic meters (MCM) per year; and (4) ~76% of afforestation regions faced ecological risks related to water resources. This study provides scientific suggestions for the sustainable development of regional water resources and afforestation. [ABSTRACT FROM AUTHOR]

Enzyme replacement therapy (ERT) is a therapeutic approach that involves the administration of specific enzymes to the patient in order to correct metabolic defects caused by enzyme deficiency. The formulation of ERTs involves the production, purification, and formulation of the enzyme into a stable and biologically active drug product, often using recombinant DNA technology. Non-systemic ERTs often involve the immobilization of the enzyme on a carrier, such as hydrogels, liposomes, or nanoparticles. ERT holds great promise for the treatment of a wide range of genetic disorders, and its success regarding lysosomal storage diseases, such as Fabry disease, Gaucher disease, and Pompe disease has paved the way for the development of similar therapies for other genetic disorders too. [ABSTRACT FROM AUTHOR]

Urban populations are rising globally, and more extreme climate events are occurring, which means more people are exposed to flood hazards such as pluvial, fluvial, coastal and compound floods. Cities located in flood-prone areas beside coasts, rivers, or both are at risk because such extreme events are often coupled with insufficient drainage capacity to offload peak discharge and withstand the surge levels. Further, the combined drivers of non-climatic factors, such as increasing urbanisation and social-economic development, and climatic drivers such as increasing extreme rainfall patterns, storms, surges, and global mean sea-level rise are unstoppable. This makes it problematic to continue to rely on improving flood protection to secure resilience. This review focuses on the lessons from recent major flood events in Europe, S Asia, E Asia, Australia, America and Africa, including the causes of the events and the post-flood responses. These responses and options are core values to understand both the importance of addressing flood resilience, by responding to floods and the explicit ways to improve risk communication among stakeholders, administration and the public which seem to be the keys to minimising flood impacts on communities. Given the continuous growth of human exposure, we suggest an urgent call for authorities to enact better flood preparation and response strategies in their flood disaster risk reduction plans and policies. This review provides implications for improving the resilience of Chinese cities and elsewhere. [ABSTRACT FROM AUTHOR]

This study was carried out to assess the effects of main and interactive groundwater salinities (GWS) (GWS1:0.38, GWS2: 5.0, and GWS3: 10 dS m−1) and groundwater depths (GWD) (GWD1: 0.30, GWD2: 0.55, and GWD3: 0.80 m) on yield and growth parameters of sweet corn. The canned seed yield in the GWS2 and GWS3 treatments reduced by 21.97% and 54.87%, respectively, as compared to the GWS1. Increasing GWS from 0.38 to 5.0 and 10.0 dS m−1 decreased aboveground fresh biomass by 16.67 and 37.95%, respectively. The groundwater contribution to ET varied from 26.80% to 87.10%, and capillary rise from groundwater to plant root zone decreased significantly with increasing GWD. The results showed that plant growth was not significantly affected under 0.38 dS m−1 GWS conditions at 0.55 m GWD, while the groundwater contribution to ET was about 52%. Stomata and SPAD values increased by 36.36% and 4.34%, respectively, in GWD3 treatment compared to GWD1 treatment. Besides, GWS decreased the leaf's K+, K+/Na+, and Ca2+/Na+ ratios at different depths. In conclusion, it can be suggested that GWD should be at least 0.80 m to achieve high sweet corn production in regions with moderately or highly saline groundwater. [ABSTRACT FROM AUTHOR]

Fluidic microphysiological systems (MPS) are microfluidic cell culture devices that are designed to mimic the biochemical and biophysical in vivo microenvironments of human tissues better than conventional petri dishes or well‐plates. MPS‐grown tissue cultures can be used for probing new drugs for their potential primary and secondary toxicities as well as their efficacy. The systems can also be used for assessing the effects of environmental nanoparticles and nanotheranostics, including their rate of uptake, biodistribution, elimination, and toxicity. Pumpless MPS are a group of MPS that often utilize gravity to recirculate cell culture medium through their microfluidic networks, providing some advantages, but also presenting some challenges. They can be operated with near‐physiological amounts of blood surrogate (i.e., cell culture medium) that can recirculate in bidirectional or unidirectional flow patterns depending on the device configuration. Here we discuss recent advances in the design and use of both pumped and pumpless MPS with a focus on where pumpless devices can contribute to realizing the potential future role of MPS in evaluating nanomaterials. This article is categorized under:Therapeutic Approaches and Drug Discovery > Emerging TechnologiesToxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials [ABSTRACT FROM AUTHOR]

Climate change and water scarcity increase the vulnerability of crop production and other ecosystem services (ES) in flood-protected lowlands under a continental climate. Restoration of wetlands leads to a higher water-buffering capacity of the landscape, strengthening various ecosystem services, and fostering adaptation to climatic, ecological, and agricultural challenges. Such restoration efforts require extensive land-use change, leading to trade-offs in provisioning and regulating ES. However, knowledge is limited about these situations, especially in the case of lowland areas. Here, we introduce a hydrological analysis in a 243 km2 flood-protected catchment in the Great Hungarian Plain, mapping the potential hydrological effects of water-retention scenarios on groundwater levels. We point out how the simulated groundwater levels will be used for estimating the changes in crop yields and tree growth (provisioning services). The introduced hydrological analysis and preliminary results for crop-yield estimates suggest a significant and scalable capacity for a nature-based hydrological adaptation: the extent of inundated areas could be increased stepwise and water retention could locally compensate dry periods due to the buffering effect of inundated meanders. [ABSTRACT FROM AUTHOR]