1. Independent and interactive effects of DOF AFFECTING GERMINATION 1 (DAG1) and the DELLA proteins GA INSENSITIVE (GAI) and REPRESSOR OF ga1-3 (RGA) in embryo development and seed germination
- Author
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Paolo Costantino, Jean-Cristophe Palauqui, Katia Belcram, Alessandra Boccaccini, Davide Capauto, Emanuele Minutello, Riccardo Lorrai, Silvia Santopolo, Paola Vittorioso, Department of Biology and Biotechnology 'Charles Darwin', Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, This work was partially supported by research grants from Ministero dell’Istruzione, Università e Ricerca, Progetti di Ricerca di Interesse Nazionale, and from Sapienza Università di Roma to PC, and from Istituto Pasteur Fondazione Cenci Bolognetti to PV., Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' [Rome], and Vittorioso, Paola
- Subjects
Arabidopsis thaliana ,[SDV]Life Sciences [q-bio] ,Mutant ,Arabidopsis ,Plant Science ,DAG1 ,GAI ,RGA ,Seed germination ,Embryogenesis ,transcription factor spatula ,arabidopsis-thaliana ,phytochrome regulation ,negative regulator ,gibberellin ,gene ,light ,expression ,dormancy ,pil5 ,régulation ,DAG1, GAI, seed germination ,MESH: Epistasis, Genetic ,MESH: Embryonic Development ,MESH: Arabidopsis ,Genetics ,Vegetal Biology ,biology ,Biologie du développement ,MESH: Transcription Factors ,Development Biology ,DNA-Binding Proteins ,Phenotype ,MESH: Repressor Proteins ,Germination ,Seeds ,Research Article ,expression des gènes ,MESH: Mutation ,Embryonic Development ,Repressor ,MESH: Arabidopsis Proteins ,MESH: Germination ,MESH: Phenotype ,DNA-binding protein ,régulateur ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Transcription factor ,Alleles ,phytochrome ,lumière ,Arabidopsis Proteins ,MESH: Alleles ,Epistasis, Genetic ,biology.organism_classification ,dormance ,Repressor Proteins ,gibbérelline ,[SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis ,MESH: Seeds ,Mutation ,Dormancy ,facteur de transcription ,Biologie végétale ,MESH: DNA-Binding Proteins ,Transcription Factors - Abstract
Background: The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of seed germination acting downstream of the master repressor PHYTOCROME INTERACTING FACTOR3-LIKE 5 (PIL5). Among others, PIL5 induces the expression of the genes encoding the two DELLA proteins GA INSENSITIVE 1 (GAI) and REPRESSOR OF ga1-3 (RGA). Results: Based on the properties of gai-t6 and rga28 mutant seeds, we show here that the absence of RGA severely increases dormancy, while lack of GAI only partially compensates RGA inactivation. In addition, the germination properties of the dag1rga28 double mutant are different from those of the dag1 and rga28 single mutants, suggesting that RGA and DAG1 act in independent branches of the PIL5-controlled germination pathway. Surprisingly, the dag1gai-t6 double mutant proved embryo-lethal, suggesting an unexpected involvement of (a possible complex between) DAG1 and GAI in embryo development. Conclusions: Rather than overlapping functions as previously suggested, we show that RGA and GAI play distinct roles in seed germination, and that GAI interacts with DAG1 in embryo development.
- Published
- 2014