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Intraerythrocytic malaria parasites reside within a parasitophorous vacuole membrane (PVM) that closely overlays the parasite plasma membrane. Although the PVM is the site of several transport activities essential to parasite survival, the basis for organisation of this membrane system is unknown. Here, we performed proximity labeling at the PVM with BioID2, which highlighted a group of single-pass integral membrane proteins that constitute a major component of the PVM proteome but whose function remains unclear. We investigated EXP1, the longest known member of this group, by adapting a CRISPR/Cpf1 genome editing system to install the TetR-DOZI-aptamers system for conditional translational control. Importantly, although EXP1 was required for intraerythrocytic development, a previously reported in vitro glutathione S-transferase activity could not account for this essential EXP1 function in vivo. EXP1 knockdown was accompanied by profound changes in vacuole ultrastructure, including apparent increased separation of the PVM from the parasite plasma membrane and formation of abnormal membrane structures. Furthermore, although activity of the Plasmodium translocon of exported proteins was not impacted by depletion of EXP1, the distribution of the translocon pore-forming protein EXP2 but not the HSP101 unfoldase was substantially altered. Collectively, our results reveal a novel PVM defect that indicates a critical role for EXP1 in maintaining proper organisation of EXP2 within the PVM.

The article presents an author's comment on the book "Teaching the Culturally Disadvantaged Pupil," which was reviewed by A. Harry Passow.

This article examines perceptions of how safe airline travel is and respondents’ level of concern over privacy and trust of authorities. These attitudes are then used to understand the choice to travel under passenger screening processes with differing levels of invasiveness. We find that travelers who are more trusting of authorities are more likely to choose to travel internationally, whereas those with low overall feelings of safety while onboard an aircraft are predisposed to not travel. Our analysis reveals what attitudes and potential screening measures are linked to this feeling of overall safety, chief among which is the presence of visible uniformed police. Our results are also novel as they study these attitudes in the context of a spate of recent air-related disasters; finding no desire to accept screening processes that invade privacy beyond what is currently practiced. [ABSTRACT FROM AUTHOR]

Large Mississippi River (MR) diversions (peak water flow >1416 m3/s and sediment loads >165 kg/s) have been proposed as part of a suite of coastal restoration projects and are expected to rehabilitate and rebuild wetlands to alleviate the significant historic wetland loss in coastal Louisiana. These coastal wetlands are undergoing increasing eustatic sea-level rise, land subsidence, climate change, and anthropogenic disturbances. However, the effect of MR diversions on wetland soil organic carbon (SOC) sequestration in receiving basins remains unknown. The rate of SOC sequestration or carbon burial in wetlands is one of the variables used to assess the role of wetland soils in carbon cycling and also to construct wetland carbon budgets. In this study, we examined the effects of MR water and sediment diversions on landscape-scale SOC sequestration rates that were estimated from vertical accretion for the next 50 yr (2010-2060) under two environmental (moderate and less optimistic) scenarios. Our analyses were based on model simulations taken from the Wetland Morphology model developed for Louisiana's 2012 Coastal Master Plan. The master plan modeled a "future-without-action" scenario as well as eight individual MR diversion projects in two of the hydrologic basins (Barataria and Breton Sound). We examined the effects that discharge rates (peak flow) and locations of these individual diversion projects had on SOC sequestration rates. Modeling results indicate that large river diversions are capable of improving basin-wide SOC sequestration capacity (162-222 g C·m-2·yr-1) by up to 14% (30 g C·m-2·yr-1) in Louisiana deltaic wetlands compared to the future-without-action scenario, especially under the less optimistic scenario. When large river diversions are placed in the upper receiving basin, SOC sequestration rates are 3.7-10.5% higher (6-24 g C·m-2·yr-1) than when these structures are placed in the lower receiving basin. Modeling results also indicate that both diversion discharge and location have large effects on SOC sequestration in low-salinity (freshwater and intermediate marshes) as compared to high-salinity marshes (brackish and saline marshes). [ABSTRACT FROM AUTHOR]

The article presents the annual report for the Journal of American History (JHA) for 2010-2011. It examines content featured in the journal during that period, including the September 2010 project "Latino History: An Interchange on Present Realities and Future Prospects,” and notes that the journal conducted interviews with several scholars, including Heather Ann Thompson, Rachel Louise Moran, and Terri L. Snyder. The author expresses gratitude to editorial board members and staff, including John Nieto-Phillips, Khalil G. Muhammad, and Kimberly M. Stanley.

Road pricing as an economic construct is not a new phenomenon in transportation research. Whilst fuel taxation and tolling of roads are common ways of raising revenue in many countries, these initiatives are primarily aimed at road infrastructure financing. Worldwide there has been growing interest in pricing structures designed to also manage the growing levels of traffic congestion and, in recent times, an increasing focus on generating reductions in carbon emissions from vehicle ownership and use. This paper presents a stated choice experiment undertaken in response to the increasing interest in the environmental externalities of travel behaviour. The aim of this choice experiment is to identify the potential to switch to more fuel efficient cars that emit lower emissions, under differing pricing and technology scenarios. Results indicate that annual and variable emissions surcharges targeted very specifically to vehicle emission rates have a noticeable role in modifying vehicle purchasing decisions. [ABSTRACT FROM AUTHOR]

The choice of automobile purchases in households often involves participation of more than one household member, each of which exerts some degree of influence on the final choice outcome. The influence of more than one agent has been recognised for many years, and yet the majority of automobile choice studies develop choice models as if a single agent is involved in the preference revelation process. What is not clear is whether it makes any substantive difference in preference revelation according to who is interviewed in a household. Using a generalised mixed logit framework that accounts for preference and scale heterogeneity, we estimate a series of models to investigate whether there are significant differences between the preferences of each individual in a household when assessed in isolation from other household members, as well as their joint preferences when expressing their preferences through a group choice task. The context is choosing amongst petrol, diesel and hybrid fuelled vehicles (associated with specific levels of fuel efficiency and engine capacity) when faced with a mix of vehicle prices, fuel prices, fixed annual registration fees, annual emission surcharges and vehicle kilometre emission surcharges. Using a stated choice experiment, we find that sampling a single individual as a representative of the household's preferences is less appropriate than utilising preference information from the relevant group of decision makers in the household. [ABSTRACT FROM AUTHOR]

This article provides a model for incorporating a student-run campus awareness week into the course objectives of a sociology class. It reflects on four semesters in which students in a sociology course on eating disorders create social change by developing and implementing a campus-based awareness week. Although the article focuses specifically on a course about eating disorders, the method may be generalized to any course in which students have the potential to educate the college community with the knowledge they have learned over the course of a semester. This article describes the course and addresses the design of the project, including student responsibilities and expectations, the specific contributions of students, and previous week themes, and it offers an evaluation of the effectiveness of this assignment. [ABSTRACT FROM PUBLISHER]