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Balanites aegyptiaca (B. aegyptiaca) is an African herb with traditional medical applications. Various pathogenic factors cause hepatic fibrosis and require novel treatment alternatives. Nanoformulation-based natural products can overcome the available drug problems by increasing the efficacy of natural products targeting disease markers. The current study investigated B. aegyptiaca methanolic extract using high-pressure liquid chromatography (HPLC), and B. aegyptiaca/chitosan nanoparticles were prepared. In vivo, evaluation tests were performed to assess the curative effect of the successfully prepared B. aegyptiaca/chitosan nanoparticles. For 30 days, the rats were divided into six groups, typical and fibrosis groups, where the liver fibrosis groups received B. aegyptiaca extract, silymarin, chitosan nanoparticles, and B. aegyptiaca/chitosan nanoparticles daily. In the current investigation, phenolic molecules are the major compounds detected in B. aegyptiaca extract. UV showed that the prepared B. aegyptiaca /chitosan nanoparticles had a single peak at 280 nm, a particle size of 35.0 ± 6.0 nm, and a negative charge at − 8.3 mV. The animal studies showed that the synthetic B. aegyptiaca/chitosan nanoparticles showed substantial anti-fibrotic protective effects against CCl4-induced hepatic fibrosis in rats when compared with other groups through optimization of biochemical and oxidative markers, improved histological changes, and modulated the expression of Col1a1, Acta2 and Cxcl9 genes, which manage liver fibrosis. In conclusion, the current research indicated that the prepared B. aegyptiaca/chitosan nanoparticles improved histological structure and significantly enhanced the biochemical and genetic markers of liver fibrosis in an animal model.

With the increasing impacts of climate change, establishing more sustainable and robust plants such as desert dates (Balanites aegyptiaca) seems to be necessary. Known for its resilience in arid conditions, this tree has the potential to become a more important food source, particularly for its potential to yield edible oil. This study characterized Balanites kernel oil (BKO) as a promising oil source in arid regions, studying the influence of geographical origin and environmental factors. Moroccan and Sudanese BKO samples were analyzed and compared with Mauritanian BKO. In the fatty acid profile, unsaturated fatty acids constituted over 70% of the BKO profile, with a predominance of linoleic acid (Li), oleic acid (Ol), palmitic acid (Pa), and stearic acid (St). Consequently, the predominant triacylglycerols were PaLiLi, PaLiOl, LiLiOl, OlLiOl, and StLiOl. α-Tocopherol dominated the tocochromanol composition (324 to 607 mg/kg), followed by γ-tocopherol (120 to 226 mg/kg), constituting 90% of the total tocochromanols. The total phytosterol content in BKO ranged from 871 to 2218 mg/kg oil, with β-sitosterol dominating (58% to 74%). Principal Component Analysis revealed that the geographical origin significantly influences BKO composition, emphasizing environmental factors, particularly water deficit and/or temperatures. Notably, Moroccan BKO collected from an area characterized by high aridity and relatively low winter temperatures, showcased a unique profile in fatty acid, phytosterols, and tocochromanols. The valorization of BKO presents an opportunity for local agricultural development in arid regions and a role model for plant development and agricultural practices in other parts of the world.

Introduction: Balanites aegyptiaca L. (DEL) is a plant used in Burkina Faso in traditional medicine to treat gastrointestinal parasitosis. The purpose of this study was to assess the in vitro anthelmintic activity of aqueous and hydro-ethanolic macerates (AM and HEM) of the fruit mesocarp from Balanites aegyptiaca against Haemonchus contortus. Methods: Phytochemical screening of the extracts was done using thin-layer chromatography. The egg hatch inhibition assay (EHIA) at 0.125, 0.15, 0.25, 0.625, 1.25, and 5 mg/mL and Adult worms mortality assay (AWMA) at 0.625, 1.25, 2.5, 5, 10, and 15 mg/mL concentrations were evaluated. Albendazole and levamisole were used as standards, and phosphate-buffered saline was used as a negative control. The antioxidant potential of extracts was evaluated using the ABTS [2,2’-azinobis (3-ethyl benzoin-6-sulphonate)] and DPPH (2,2-diphenyl-picrylhydrazine) methods and Fe3+ ion reducing power. The standards used were trolox and ascorbic acid. Results: Several secondary metabolites such as sterols, triterpenes, tannins, flavonoids, coumarins, and saponins were identified. For EHIA, the AM was more effective than HEM with 50% inhibitory concentrations (IC50) of 0.35 mg/mL and 0.43 mg/mL, respectively. AM was even more effective for AWMA than HEM, with 50% lethal concentrations (LC50) of 3.15 mg/mL and 8.37 mg/mL, respectively. The DPPH method gave an IC50 of 834.55 μg/mL and the capacity to reduce ferric ion (Fe3+) to ferrous ion (Fe2+) was 270.4 µmol/Ascorbic acid equivalent. Conclusion: AM and HEM from the mesocarp of B. aegyptiaca have anthelmintic properties. AM is more effective than HEM, which justifies their use with priority for AM.

Several attempts have been made for green synthesis of copper oxide nanoparticles (CuO-NPs) using different plant extracts. The present study revealed that, antibacterial and CuO-NPs were synthesized using ecofriendly and non-toxic Balanites aegyptiaca stem bark extract. The biosynthesized CuO-NPs were characterized by using UV–Vis analysis, Fourier Transform Infrared analysis (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray analysis (EDX) and Transmission Electron Microscopy (TEM) analysis. In addition, the antibacterial activity of green synthesized CuO-NPs was tested using the agar well diffusion method against four bacterial pathogens “viz”: E. coli, V. cholerae, B. substilis and E. faecalis, which were responsible for causing severe infectious diseases in human being. CuO NPs exhibited enhanced antibacterial activity against the pathogen of B. subtilis i.e. 13 mm. Hence, CuO-NPs have the potential to be used as bactericidal component.

Phenytoin-induced liver injury (PHT ILII) is a serious condition that may necessitate discontinuation of the drug. This study investigates the mechanisms of PHT ILII and evaluates the protective effects of Balanites Aegyptiaca (BA) fruit extracts on the liver. We focus on the Nrf2/MAPK/NF-κB/Beclin-1 signaling pathways involved in oxidative stress and inflammation from drug-induced liver injury. Phytochemical analyses of BA fruit extracts (Bu-F and EA-F) are conducted. Molecular docking techniques explore the interaction between phenytoin (PHT) and the Nrf2/MAPK/NF-κB/Beclin-1 pathways. Thirty-six male rats are divided into Control, Bu-F, EA-F, PHT, Bu-F/PHT, and EA-F/PHT groups, and they are observed for 45 days. EA-F extract is rich in phenolics/flavonoids, while Bu-F extract mainly contains saponins.PHT ILII causes histological damage in liver tissues and affects Nrf-2, MAPK, TNF-α, IL-1β, Mcp-1, Beclin-1, iNOS expression, and liver function markers (ALT, AST, ALP). However, EA-F/Bu-F extracts effectively improve the histological structure and significantly reduce biochemical/immunohistochemical parameters, restoring them to near-normal levels. EA-F extract is particularly effective.In conclusion, the Nrf2/MAPK /Beclin-1 pathways play a critical role in the development of PHT ILII. BA fruit extracts show promise as hepato-protective agents, with the EA-F extract demonstrating superior efficacy. These results lay the groundwork for new treatments for PHT ILII and drug-induced liver injuries.

The Balanites aegyptiaca tree is widely used in many countries where it occurs. However, in Morocco its nutritional properties and chemical composition have not been extensively studied. This study aims to investigate its potential health benefits, focusing on bioactive compounds found in the fruit, including the pulp, shell and kernel. The fatty acid composition, phytosterol content, triacylglycerols and tocopherol levels of the lipid fraction extracted from different parts of Balanites fruit were analyzed using chromatographic techniques. Additionally, the antioxidant activity was assessed through the DPPH, ABTS, and FRAP assays. Results showed that the pulp, shell and kernel are rich in oleic acid, with percentages of 47.2%, 45.9%, and 41.9%, respectively. The linoleic acid content was consistent across all three parts. The shell had the highest phytosterols content, with β‐sitosterol being the most abundant in all three parts. The analysis revealed some variability in the identification of tocopherols, with the kernel containing the highest content of tocopherols, mainly α‐Tocopherol at 399 mg kg−1. The fruit, in its entirety, exhibits antioxidant activity as evaluated by three different methods: DPPH, ABTS, and FRAP. Overall, the study demonstrates that the fruit of B. aegyptiaca growing in Morocco possesses properties that could be utilized in the food and medical industries. Practical Application: The fruit parts of the Balanites tree (Balanites aegyptiaca) are rich in fatty acids, tocochromanols, and phytosterols, exhibiting significant antioxidant capacity. This study reveals the potential of the bioactive compounds of the fruit, which offers substantial practical applications as a natural source of antioxidants in food formulations and nutraceuticals. By leveraging the bioactive compounds identified in this study, researchers and industries can investigate the development of functional food and cosmeceuticals formulations to enhance human health and well‐being. [ABSTRACT FROM AUTHOR]

Abstract Background Balanites aegyptiaca (L.) Delile, commonly known as desert date, is a thorny evergreen tree belonging to the family Zygophyllaceae and subfamily Tribuloideae that is widespread in arid and semiarid regions. This plant is an important source of food and medicines and plays an important role in conservation strategies for restoring degraded desert ecosystems. Results In the present study, we sequenced the complete plastome of B. aegyptiaca. The chloroplast genome was 155,800 bp, with a typical four-region structure: a large single copy (LSC) region of 86,562 bp, a small single copy (SSC) region of 18,102 bp, and inverted repeat regions (IRa and IRb) of 25,568 bp each. The GC content was 35.5%. The chloroplast genome of B. aegyptiaca contains 107 genes, 75 of which coding proteins, 28 coding tRNA, and 4 coding rRNA. We did not observe a large loss in plastid genes or a reduction in the genome size in B. aegyptiaca, as found previously in some species belonging to the family Zygophyllaceae. However, we noticed a divergence in the location of certain genes at the IR-LSC and IR-SSC boundaries and loss of ndh genes relative to other species. Furthermore, the phylogenetic tree constructed from the complete chloroplast genome data broadly supported the taxonomic classification of B. aegyptiaca as belonging to the Zygophyllaceae family. The plastome of B. aegyptiaca was found to be rich in single sequence repeats (SSRs), with a total of 240 SSRs. Conclusions The genomic data available from this study could be useful for developing molecular markers to evaluate population structure, investigate genetic variation, and improve production programs for B. aegyptiaca. Furthermore, the current data will support future investigation of the evolution of the family Zygophyllaceae.

Activated carbon is an attractive adsorbent for capturing various environmental pollutants, including CO 2 . Herein, an optimal synthesis and impressive performance of activated carbon made from Balanites aegyptiaca (Desert date) seed shells is reported, which is an abundant agricultural waste in the Middle East and Africa. The synthesis route involved pretreating the biomass with KOH and heating it under a suitable temperature profile. An optimal KOH-to-biomass ratio and multi-stage carbonization yielded activated carbon with a surface area above 3000 m 2 /g and an average pore size of nearly 4.1 nm. At 0 °C, this activated carbon exhibited CO 2 uptake of 11.3 mmol g -1 that surpassed the uptake capacity of previously reported activated carbons. The selectivity towards CO 2 was also found to be significantly higher compared to other gases. Thus, the present approach demonstrates an efficient conversion of agricultural waste to activated carbon for capturing CO 2 and other environmental contaminants., (© 2024 Wiley-VCH GmbH.)

Activated carbon is an attractive adsorbent for capturing various environmental pollutants, including CO2. Herein, an optimal synthesis and impressive performance of activated carbon made from Balanites aegyptiaca (Desert date) seed shells is reported, which is an abundant agricultural waste in the Middle East and Africa. The synthesis route involved pretreating the biomass with KOH and heating it under a suitable temperature profile. An optimal KOH‐to‐biomass ratio and multi‐stage carbonization yielded activated carbon with a surface area above 3000 m2/g and an average pore size of nearly 4.1 nm. At 0 °C, this activated carbon exhibited CO2 uptake of 11.3 mmol g−1 that surpassed the uptake capacity of previously reported activated carbons. The selectivity towards CO2 was also found to be significantly higher compared to other gases. Thus, the present approach demonstrates an efficient conversion of agricultural waste to activated carbon for capturing CO2 and other environmental contaminants. [ABSTRACT FROM AUTHOR]

Microsporum gypseum is a dermatophyte with a geophilic nature that is found all over the globe. It mainly causes tinea in the scalp, arms, and legs in humans. Squalene epoxidase (SE) is a crucial enzyme in M. gypseum for the biosynthesis of ergosterol. The medicinal plant Balanites aegyptiaca is an abundant supply of secondary constituents with great therapeutic values. In this research, the fruit epicarp portion was used to inhibit M. gypseum using experimental and computational techniques. The anti-dermatophytic activity of epicarp extracts on M. gypseum was evaluated using the poison plate method at five different concentrations. At 3 mg/mL, the M. gypseum was completely controlled by the fractioned chloroform extract of epicarp. The compounds from previous research were utilized for docking studies (Abuthakir et al., 2022). The ideal compounds and the drug terbinafine were then docked using Schrödinger’s Glide module. It demonstrates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside outperforms other substances and the drug terbinafine in docking analysis. Desmond, Schrödinger Molecular Dynamics simulations were also performed for (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside-squalene epoxidase complexes. The complex appears to be more stable, according to the MD simulation research. This study indicates that (3E)-7-Hydroxy-3,7-dimethyl-3-octen-1-yl-6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside could be used as a potential inhibitor of M. gypseum growth, and it could be studied further.

The Balanites fruits were used to develop a yellow sparkling indigenous wine, using the musts supplemented with sucrose at 5 % (BaW5), 10 % (BaW10), and 20 % (BaW20). The physicochemical parameters were assessed to monitor the changes during 6-day fermentation; and the physicochemical, sensory, and microbiological properties of the formulated wines were evaluated through 4-week ageing. During fermentation, total soluble solids, specific gravity and pH were negatively correlated to alcohol content (% v/v). Changes in physicochemical properties of aged wines were significant (p

With the increasing impacts of climate change, establishing more sustainable and robust plants such as desert dates ( Balanites aegyptiaca ) seems to be necessary. Known for its resilience in arid conditions, this tree has the potential to become a more important food source, particularly for its potential to yield edible oil. This study characterized Balanites kernel oil (BKO) as a promising oil source in arid regions, studying the influence of geographical origin and environmental factors. Moroccan and Sudanese BKO samples were analyzed and compared with Mauritanian BKO. In the fatty acid profile, unsaturated fatty acids constituted over 70% of the BKO profile, with a predominance of linoleic acid (Li), oleic acid (Ol), palmitic acid (Pa), and stearic acid (St). Consequently, the predominant triacylglycerols were PaLiLi, PaLiOl, LiLiOl, OlLiOl, and StLiOl. α-Tocopherol dominated the tocochromanol composition (324 to 607 mg/kg), followed by γ-tocopherol (120 to 226 mg/kg), constituting 90% of the total tocochromanols. The total phytosterol content in BKO ranged from 871 to 2218 mg/kg oil, with β-sitosterol dominating (58% to 74%). Principal Component Analysis revealed that the geographical origin significantly influences BKO composition, emphasizing environmental factors, particularly water deficit and/or temperatures. Notably, Moroccan BKO collected from an area characterized by high aridity and relatively low winter temperatures, showcased a unique profile in fatty acid, phytosterols, and tocochromanols. The valorization of BKO presents an opportunity for local agricultural development in arid regions and a role model for plant development and agricultural practices in other parts of the world.

This study describes the biosynthesis of silver nanoparticles (AgNPs) using Balanites aegyptiaca (B. aegyptiaca) leaf extract. The biosynthesized AgNPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), X-ray diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy with (SEM-EDS). The AgNPs showed an average size of 10–20 nm, spherical shape, and crystalline nature. The application of these synthesized AgNPs to dye degradation showed that the AgNPs removed the two organic pollutants methylene blue (MB, 93.47%) and congo red (CR, (78.57%). In vitro investigation of the antifungal activity of the AgNPs against Fusarium oxysporum, a phytopathogenic fungus, showed a maximum percent radial growth inhibition of 82.00 ± 1.00% and a spore percent inhibition of 73.66 ± 3.94 for 150 μg/ml of biosynthesized AgNPs.

Biodiesel is a domestically produced, clean-burning, renewable substitute for petroleum diesel. Using biodiesel as a vehicle fuel increases energy security, improves air quality and the environment, and provides safety benefits. . This study extracted, characterized and determined the physicochemical properties of biodiesel produced from Desert date (B. aegyptiaca) seed oil using Soxhlet extraction method with n-hexane as the extraction solvent. The oil obtained was characterized using FTIR and GC-MS analyses and its physicochemical properties determined. The oil obtained was subsequently transesterified using methanol and KOH catalyst. The obtained biodiesel was characterized and its physicochemical properties determined. The result showed that B. aegyptiaca seed oil had density of 0.9 g/cm3, viscosity of 6 cSt at 40 0C and viscosity of 2.5 cSt at 100 0C. The cloud and pour points of the oil was 21 and 9 0C respectively. The oil comprised of eleven fatty acids; predominantly linoleic acid (21.88 %), vaccenic acid (13.90 %) and palmitic acid (10.96 %). The biodiesel had density of 0.88 g/cm3, kinematic viscosity of 4.2 cSt, cloud and pour points of 16 0C and 7 0C respectively. The biodiesel also had free fatty acid (FFA) of 0.79 %, iodine value of 42 mgI2/100g and saponification value of 176. The biodiesel primarily consisted of 9,12-Octadecadienoic acid methyl ester (45.46 %), Hexadecanoic acid methyl ester (20.12 %), Butyl-9,12-Octadecadienoate (12.43 %) and Heptadecanoic acid-16-methyl ester (10.43 %). The properties obtained for the biodiesel were comparable to those of biodiesels reported in literature and thus, shows remarkable potentials to be used as substitute to fossil-based diesel fuel.

Background and Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed. Materials and Methods: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen. Results: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell. Conclusion: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris–egg yolk–based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest.

This study involved analysing the carbon-13 isotope ratio (δ13C) of cellulose, lignin, and whole wood powder of Balanites aegyptiaca to determine the optimal substrate for future ecophysiological studies. From seven different trees (i.e., three from the Sudanian zone and four from the Chadian Sahelian zone), 21 samples were taken for this study. Identical variation trends were observed with cellulose, lignin, and whole wood powder, with δ13C values for cellulose always higher than for the other substrates, although the trees were located under different climatic conditions. The means obtained were −26.0 ‰, −28.7 ‰, and −27.1 ‰, for cellulose, lignin, and whole wood powder, respectively. Spearman's correlation test, which was used to analyse relationships between the results, revealed a strong correlation (r2 = 0.93) between the δ13C cellulose values and those of whole wood powder from B. aegyptiaca, as well as between the δ13C cellulose values and those of lignin (r2 = 0.73). This strong correlation between cellulose and wood powder results, and between cellulose and lignin, suggests that the isolation of cellulose is not necessary for δ13C analysis of B. aegyptiaca wood. [ABSTRACT FROM AUTHOR]

Climate change is one of the biggest challenges facing the planet today. It is important to ensure access to energy to promote quality of life and economic development. It is therefore essential to address this problem in sustainable development. Advances in the development of new technologies have given hope that the goals will be attainable in the field of energy. Currently, biotechnological processes make it possible to promote this fruit of the desert date tree and make available to citizens a new generation of products with high added value such as bioethanol. In this context, various alcoholic fermentation tests at the laboratory level are carried out to determine the dilution rate, in order to optimize the process. We compared the degree of crude alcohol of four dilutions, i.e. the dilution by (six, nine, ten and eleven times) of the mass (110) g of the Balanites fruits pulp. We obtained after the distillation step, the following optimization results for the fermentation of the four substrates at different dilution than the 10-fold dilution of the substrate gave the best yield.