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Abstract Aim  The clinical usefulness of 2-deoxy-2-[F-18]fluoro-D-glucose-positron emission tomography (FDG-PET) in head and neck squamous cell carcinoma (HNSCC) is now well-documented. However, its sensitivity is greater than its specificity due to false-positive results in inflammatory or infectious lesions, which are frequent in this area, in particular after treatment by surgery and/or radiotherapy. O-2-fluoro-(18F)-ethyl-L-thyrosine (FET) has been reported not to be taken up by such lesions, and a preliminary study indicated that this may be clinically useful in HNSCC. We performed a prospective study to compare the diagnostic performances of FDG and FET PET/CT in the different settings of HNSCC. Materials and Methods  Twenty-seven patients (20 men and seven women, aged 48–76, among 30 patients included) and 69 suspected cancer sites are now evaluable on basis of postsurgical histology and/or follow-up greater than 6 months; 15 patients were referred for initial staging and 12 during posttherapy follow-up, a recurrence being suspected in eight of them. FDG and FET PET/CT were performed on two different days, the patient fasting for 6 h, 1 h after injection of 5 MBq/kg of body mass of each radiopharmaceutical. Both PET/CT examinations were blind read more than 6 months after the end of inclusions in a random order for each tracer and with a time interval greater than 1 month between FDG and FET PET/CT blind readings. Results  Overall diagnostic performances, derived from blind reading: FDG PET/CT on a per patient basis: sensitivity 100%, specificity 71%, accuracy 93%; FDG PET/CT on a per site basis: sensitivity 95%, specificity 63%, accuracy 83%; FET PET/CT on a per patient basis: sensitivity 70%, specificity 100%, accuracy 78%; FET PET/CT on a per site basis: sensitivity 64%, specificity 100%, accuracy 78%. At site level, sensitivity was significantly greater with FDG (p p  Conclusion  Although its good specificity was confirmed, FET did not appear to be suited as a first-line PET tracer in HNSCC imaging and cannot replace FDG for staging due to insufficient sensitivity. However, it was useful in a few selected cases to favor a wait and see attitude when a FDG FET− focus was discovered in patients referred for systematic FDG PET during follow-up. In contrast, second primary cancers should not be ruled out if FDG was clearly positive in the lungs or the digestive tract. [ABSTRACT FROM AUTHOR]

This study addresses how salience shapes the perceptual organization of an auditory scene. A psychophysical task that was introduced previously by Susini, Jiaouan, Brunet, Houix, and Ponsot [(2020). Sci. Rep. 10(1), 16390] was adapted to assess how the ability of non-musicians and expert musicians to detect local/global contour changes in simple hierarchically-organized tone sequences is affected by the relative salience of local information in the timbre dimension. Overall, results show that salience enhanced local processing capacities, at the cost of global processing, suggesting a bottom-up reallocation of attention. Interestingly, for non-musicians, salience caused a reversal of the basic global-over-local processing prioritization as it is typically observed in expert musicians. [ABSTRACT FROM AUTHOR]

The ability to manufacture complex 3D structures with nanometer-scale resolution, such as Fresnel Zone Plates (FZPs), is crucial to achieve state-of-the-art control in X-ray sources for use in a diverse range of cutting-edge applications. This study demonstrates a novel approach combining Electron Beam Lithography (EBL) and cryoetching to produce silicon-based FZP prototypes as a test bench to assess the strong points and limitations of this fabrication method. Through this method, we obtained FZPs with 100 zones, a diameter of 20 µm, and an outermost zone width of 50 nm, resulting in a high aspect ratio that is suitable for use across a range of photon energies. The process incorporates a chromium mask in the EBL stage, enhancing microstructure precision and mitigating pattern collapse challenges. This minimized issues of under- and over-etching, producing well-defined patterns with a nanometer-scale resolution and low roughness. The refined process thus holds promise for achieving improved optical resolution and efficiency in FZPs, making it viable for the fabrication of high-performance, nanometer-scale devices. [ABSTRACT FROM AUTHOR]

Binaural audio recordings become increasingly popular in multimedia repositories, posing new challenges in indexing, searching, and retrieval of such excerpts in terms of their spatial audio scene characteristics. This paper presents a new method for the automatic estimation of one of the most important spatial attributes of binaural recordings of music, namely "ensemble width." The method has been developed using a repository of 23,040 binaural excerpts synthesized by convolving 192 multi-track music recordings with 30 sets of head-related transfer functions (HRTF). The synthesized excerpts represented various spatial distributions of music sound sources along a frontal semicircle in the horizontal plane. A binaural auditory model was exploited to derive the standard binaural cues from the synthesized excerpts, yielding a dataset representing interaural level and time differences, complemented by interaural cross-correlation coefficients. Subsequently, a regression method, based on gradient-boosted decision trees, was applied to the formerly calculated dataset to estimate ensemble width values. According to the obtained results, the mean absolute error of the ensemble width estimation averaged across experimental conditions amounts to 6.63° (SD 0.12°). The accuracy of the method is the highest for the recordings with ensembles narrower than 30°, yielding the mean absolute error ranging between 0.8° and 10.2°. The performance of the proposed algorithm is relatively uniform regardless of the horizontal position of an ensemble. However, its accuracy deteriorates for wider ensembles, with the error reaching 25.2° for the music ensembles spanning 90°. The developed method exhibits satisfactory generalization properties when evaluated both under music-independent and HRTF-independent conditions. The proposed method outperforms the technique based on "spatiograms" recently introduced in the literature. [ABSTRACT FROM AUTHOR]

Multilevel diffractive lenses (MDLs) has undergone considerable advancements, marked by their exceptional efficiency and diverse focusing capabilities, resulting in their widespread use in optical systems. In recent times, MDLs have consistently been juxtaposed with metalenses, which have experienced swift progress over the last decade. Concurrently, MDLs have continued to evolve, propelled by their distinct advantages, such as cost-effective production and adaptability for mass manufacturing. This article explores the evolution and foundational concepts of MDLs, highlighting the advantages of their circular symmetry in enhancing simulation and optimization efficiency. Furthermore, we present several innovative fabrication methods for MDLs that capitalize on the latest advancements in 3D printing technology. We also show the practical applications and potential future developments of MDLs. [ABSTRACT FROM AUTHOR]

Objective: The objective of this work was to conduct a systematic study to evaluate the performance of citric acid (CA), an example of a commonly used organic acid in hair cosmetics, on different types of chemically treated hair and to better understand how CA works., Methods: Consumer-centric routines were used to prepare the chemically treated hair, namely high-lift bleached, middle-lift bleached, permanently coloured and a combination of permed and coloured hair. Hair was subsequently treated with CA and hair reinforcement was investigated by physical techniques: High Pressure Differential Scanning Calorimetry (HPDSC), Miniature Tensile Test (MTT), Cyclic Fatigue Tensile Test (CFTT) & Micro X-ray Diffraction (μXRD) as well as chemical analysis (elemental, amino acids)., Results: CA treatment demonstrably enhances the structural integrity of chemically treated hair. DSC revealed increased crosslinking density of keratin-associated proteins (KAPs) in the hair cortex, evidenced by a 6.7% to 15.0% increase in denaturation temperature, varying with the specific chemical treatment. This improvement translates to enhanced mechanical properties, with tensile elastic modulus increasing by 7.5% to 23.5% and fatigue resistance (median cycles to break) improving by a remarkable 56% to 124%. Furthermore, CA treatment significantly reduced hair calcium levels by 21% to 42%. XRD analysis confirmed a reduction in the structure and quantity of crystallized calcium soaps following CA treatment. The observed performance improvements likely stem from a combination of mechanisms, including calcium chelation, the treatment's pH, and reinforcement of the non-covalent protein network. The precise interplay of these factors appears to be influenced by the type of pre-existing chemical treatment on the hair., Conclusion: CA effectively reinforces hair damaged by various common chemical treatments. However, these findings highlight the complex relationship between hair damage, chemical treatment type, and the efficacy of reinforcement strategies. The observed dependence of CA's performance on the level of pre-existing damage underscores the need for further research into developing targeted actives for consumers with specific and severe hair damage profiles. This opens exciting avenues for innovation in hair care tailored to individual needs., (© 2025 Society of Cosmetic Scientists and Societe Francaise de Cosmetologie.)

Simple Summary: Among head–neck tumors, squamous cell carcinoma is the most frequent histotype and includes a range of malignancies with different sites of origin as well as different therapeutic strategies and clinical outcomes. In daily practice, patients with head–neck squamous cell carcinoma are seen in various clinical settings, requiring a multidisciplinary approach to therapeutic decisions and clinical care. 18F-FDG PET/CT plays a well-defined role in the management of these tumors for pre-treatment staging and radiotherapy planning as well as treatment-response assessment and post-therapy follow-up. This paper is an overview of the standard use of 18F-FDG PET/CT in the various clinical scenarios of head–neck squamous cell carcinoma. Also, emerging applications will be reviewed, including the use of radiopharmaceuticals other than 18F-FDG, PET/MRI implementation in clinical practice, and the use of radiomics and machine learning. This article provides an overview of the use of 18F-FDG PET/CT in various clinical scenarios of head–neck squamous cell carcinoma, ranging from initial staging to treatment-response assessment, and post-therapy follow-up, with a focus on the current evidence, debated issues, and innovative applications. Methodological aspects and the most frequent pitfalls in head–neck imaging interpretation are described. In the initial work-up, 18F-FDG PET/CT is recommended in patients with metastatic cervical lymphadenectomy and occult primary tumor; moreover, it is a well-established imaging tool for detecting cervical nodal involvement, distant metastases, and synchronous primary tumors. Various 18F-FDG pre-treatment parameters show prognostic value in terms of disease progression and overall survival. In this scenario, an emerging role is played by radiomics and machine learning. For radiation-treatment planning, 18F-FDG PET/CT provides an accurate delineation of target volumes and treatment adaptation. Due to its high negative predictive value, 18F-FDG PET/CT, performed at least 12 weeks after the completion of chemoradiotherapy, can prevent unnecessary neck dissections. In addition to radiomics and machine learning, emerging applications include PET/MRI, which combines the high soft-tissue contrast of MRI with the metabolic information of PET, and the use of PET radiopharmaceuticals other than 18F-FDG, which can answer specific clinical needs. [ABSTRACT FROM AUTHOR]

Objectives: This study aims to examine the effects of autologous platelet-rich plasma (PRP), which increases new connective tissue synthesis and revascularisation, on healing in parotid surgery wounds, prevention of salivary fistula formation, drain removal time and hospitalisation in the postoperative period., Materials and Methods: Fifty-four patients who had an operation on partial parotidectomy were randomised, and then two groups were created. PRP was obtained by centrifuging the blood taken from the patients in the study group at the end of the surgery. This obtained PRP was injected into the surgical site, and then the wound flap was closed by suturing. Patients were evaluated for parameters such as the development of salivary fistula, duration of drain removal, discharge time and all other complications during the postoperative 4 weeks., Results: Drain removal and discharge times of the PRP group cases were statistically shorter than those in the control group. The rate of development of a salivary fistula was remarkably high in the control group, but it was not statistically significant. A statistically significant correlation was found between the location of the compared tumour, the volume of material removed and the incidence of all complications., Conclusions: PRP reduced the duration of drain removal and discharge times for those who had an operation on partial parotidectomy. Thus, the decreased discharge time provides both reduced health costs and reduced risk of developing nosocomial infections. Although it was not statistically significant, a significant difference was observed in the rates of salivary fistula development., (© 2024 John Wiley & Sons Ltd.)

The problem of characterizing voice quality has long caused debate and frustration. The richness of the available descriptive vocabulary is overwhelming, but the density and complexity of the information voices convey lead some to conclude that language can never adequately specify what we hear. Others argue that terminology lacks an empirical basis, so that language-based scales are inadequate a priori. Efforts to provide meaningful instrumental characterizations have also had limited success. Such measures may capture sound patterns but cannot at present explain what characteristics, intentions, or identity listeners attribute to the speaker based on those patterns. However, some terms continually reappear across studies. These terms align with acoustic dimensions accounting for variance across speakers and languages and correlate with size and arousal across species. This suggests that labels for quality rest on a bedrock of biology: We have evolved to perceive voices in terms of size/arousal, and these factors structure both voice acoustics and descriptive language. Such linkages could help integrate studies of signals and their meaning, producing a truly interdisciplinary approach to the study of voice. [ABSTRACT FROM AUTHOR]

Marine Protected Areas (MPAs) are vital for biodiversity conservation, yet their effectiveness in preserving foundation seaweeds remains understudied. This study investigates the diversity and distribution of Cystoseira sensu lato (including Cystoseira, Ericaria, and Gongolaria, hereafter referred to as Cystoseira s.l.) populations in an MPA located in the central Mediterranean Sea, comparing them with those in two unprotected sites. We hypothesized MPA Cystoseira s.l. populations would display higher diversity and structure compared to outside unprotected sites. Results revealed a total of 19 Cystoseira s.l. species at depths of 0–20 m, with the MPA exhibiting a higher diversity than unprotected sites. Thus, MPAs can play a crucial role in fostering the diversity of Cystoseira s.l. populations. However, no significant differences were observed among the MPA's protection zones, raising questions about the zoning effectiveness. Additionally, our survey uncovered a substantial presence of non-indigenous seaweeds within the MPA. In conclusions, while MPAs improved Cystoseira s.l. diversity compared to unprotected sites, the varying efficacy of protection within MPA zones suggested a necessity for site-specific conservation strategies. The presence of non-indigenous seaweeds emphasizes ongoing challenges. This study provides a baseline for understanding Cystoseira s.l. population dynamics, crucial for future monitoring and conservation efforts in the face of global change. [ABSTRACT FROM AUTHOR]

Auditory salience is a fundamental property of a sound that allows it to grab a listener's attention regardless of their attentional state or behavioral goals. While previous research has shed light on acoustic factors influencing auditory salience, the semantic dimensions of this phenomenon have remained relatively unexplored owing both to the complexity of measuring salience in audition as well as limited focus on complex natural scenes. In this study, we examine the relationship between acoustic, contextual, and semantic attributes and their impact on the auditory salience of natural audio scenes using a dichotic listening paradigm. The experiments present acoustic scenes in forward and backward directions; the latter allows to diminish semantic effects, providing a counterpoint to the effects observed in forward scenes. The behavioral data collected from a crowd-sourced platform reveal a striking convergence in temporal salience maps for certain sound events, while marked disparities emerge in others. Our main hypothesis posits that differences in the perceptual salience of events are predominantly driven by semantic and contextual cues, particularly evident in those cases displaying substantial disparities between forward and backward presentations. Conversely, events exhibiting a high degree of alignment can largely be attributed to low-level acoustic attributes. To evaluate this hypothesis, we employ analytical techniques that combine rich low-level-mappings from acoustic profiles with high-level embeddings extracted from a deep neural network. This integrated approach captures both acoustic and semantic attributes of acoustic scenes along with their temporal trajectories. The results demonstrate that perceptual salience is a careful interplay between low-level and high- level attributes that shapes which moments stand out in a natural soundscape. Furthermore, our findings underscore the important role of longer-term context as a critical component of auditory salience, enabling us to discern and adapt to temporal regularities within an acoustic scene. The experimental and model- based validation of semantic factors of salience paves the way for a complete understanding of auditory salience. Ultimately, the empirical and computational analyses have implications for developing large-scale models for auditory salience and audio analytics. [ABSTRACT FROM AUTHOR]

The worldwide decline of macroalgal forests is raising major concerns for the potentially negative consequences on biodiversity and ecosystem functions, pushing for the definition of specific conservation and restoration measures. Protecting and restoring these habitats requires detailed information on their distribution, ecological status, and drivers of decline. Here, we provide the most updated available information on the distribution of Mediterranean Cystoseira s.l. forests by conducting a comprehensive bibliographic survey of literature published from 2009 to 2021, complemented by unpublished data. We also provide insights into the ecological status of these forests and the stressors affecting them across the Mediterranean basin. Our results show that most Mediterranean coasts remain un(der)studied and that the available information is concentrated in spatially limited coastal areas, restricted to very few species. When the ecological status is reported, data is highly heterogeneous, making any comparisons problematic, what claims for the description and use of easy and standardized monitoring methods for comparative purposes. Drivers of decline of Cystoseira s.l. forest have been even less investigated and, therefore, still poorly characterized. Our results highlight that our current knowledge is still insufficient to implement effective conservation and restoration strategies at the basin scale but also regionally. We call for the urgent need for mapping and standardized monitoring of Cystoseira s.l. forests to obtain baseline information for future management strategies involving their conservation, the mitigation of the stressors threatening them and the restoration of the degraded forests. [ABSTRACT FROM AUTHOR]

This study explored the biodiversity of Fucales along the islands of Lipari and Vulcano, almost ten years after the last studies on the phytobenthos of these islands. To carry out this research, a monitoring activity was conducted through both scuba dives and snorkelling activities. Through this study, a total of 13 species (four belonging to Cystoseira, three to Ericaria, two to Gongolaria, and four to Sargassum) was observed. When comparing the present data with past studies, nine species were previously reported, ten taxa were not found in the present study, and four taxa were reported here for the first time in the Aeolian Archipelago. The alpha (local) diversity was higher in Vulcano than in Lipari. Moreover, the beta (turnover) diversity showed a different assemblage in species grouping between the two islands. Since some of the observed species are currently considered rare or in regression, their finding in the Aeolian Archipelago is of particular interest. Therefore, they should receive special consideration, particularly in light of the possible future establishment of a marine protected area (MPA) in the Aeolian Archipelago. [ABSTRACT FROM AUTHOR]

The habitation and environment are affected by the stable isotopes of caesium (Cs) and strontium (Sr), as well as by their radioactive isotopes. The current work gives insight on Alstonia scholaris' capacity to phytoextract stable caesium (Cs) and strontium (Sr), as well as the plant's ability to protect against the toxicity of both elements. Experiments with Cs [0–5 mM (CsCl)] and Sr [0–3 mM (SrCl2. 6H2O)] dosing in controlled light, temperature, and humidity condition in greenhouse for 21 days were undertaken. Cs and Sr accumulation in different plant parts was quantified with atomic absorption spectroscopy (AAS) and inductively coupled plasma-optical emission spectrometry (ICP-OES) respectively. Hyper-accumulation capacity for Cs and Sr was estimated with indices like transfer factor (TF) and translocation factors (TrF). The uptake pattern of caesium in Alstonia scholaris is 5452.8–24,771.4 mg/kg DW (TF = 85.2–57.6) and in the case of Sr is 1307.4–8705.7 mg/kg DW (TF = 85.3–1.46). The findings demonstrated the plant's ability to transfer Cs and Sr to aboveground biomass on the basis of dry weight, with the majority of the metals being deposited in the shoot rather than the root portion of the plant. For Cs and Sr, with increasing concentration, the plants exhibited the enzymatic expression for defence against metal toxicity by free radicals compared to control. Field emission electron microscopy with energy-dispersive spectroscopy (FESEM with EDS) was employed to assess the spatial distribution of Cs and Sr in plant leaf, indicating the accumulation of Cs, Sr, and their homologous components. [ABSTRACT FROM AUTHOR]

Attention allows the listener to select relevant information from their environment, and disregard what is irrelevant. However, irrelevant stimuli sometimes manage to capture it and stand out from a scene because of bottom-up processes driven by salient stimuli. This attentional capture effect was observed using an implicit approach based on the additional singleton paradigm. In the auditory domain, it was shown that sound attributes such as intensity and frequency tend to capture attention during auditory search (cost to performance) for targets defined on a different dimension such as duration. In the present study, the authors examined whether a similar phenomenon occurs for attributes of timbre such as brightness (related to the spectral centroid) and roughness (related the amplitude modulation depth). More specifically, we revealed the relationship between the variations of these attributes and the magnitude of the attentional capture effect. In experiment 1, the occurrence of a brighter sound (higher spectral centroid) embedded in sequences of successive tones produced significant search costs. In experiments 2 and 3, different values of brightness and roughness confirmed that attention capture is monotonically driven by the sound features. In experiment 4, the effect was found to be symmetrical: positive or negative, the same difference in brightness had the same negative effect on performance. Experiment 5 suggested that the effect produced by the variations of the two attributes is additive. This work provides a methodology for quantifying the bottom-up component of attention and brings new insights on attention capture and auditory salience. [ABSTRACT FROM AUTHOR]

Fucales (Phaeophyceae) are ecosystem engineers and forest-forming macroalgae whose populations are declining dramatically. In the Mediterranean Sea, Cystoseira sensu lato (s.l.)—encompassing the genera Cystoseira sensu stricto, Ericaria, and Gongolaria—is the most diverse group, and many species have been shown to be locally extinct in many areas, resulting in a shift toward structurally less complex habitats with the consequent loss of ecosystem functions and services. In this study, we report on the extensive occurrence of healthy and dense marine forests formed by Fucales in the Santa Maria di Castellabate Marine Protected Area in Cilento, Italy (Tyrrhenian Sea, Mediterranean). On a total area of 129.45 ha, 10 Cystoseira s.l. taxa were detected using a combined morphological and molecular approach, with an average cover of more than 70%. One of these taxa has been sequenced for the first time. These findings underline the high ecological value of this area as a hotspot of benthic biodiversity and highlight the importance of marine protected area management and regional monitoring programs to ensure the conservation of these valuable yet fragile coastal ecosystems. [ABSTRACT FROM AUTHOR]

The uptake of radiocesium (RCs) by plants is key to the assessment of its environmental risk. However, the transfer process of RCs in the water-vegetable system still remains unclear. In this work, the uptake and accumulation processes of Cs+ (0-10 mM) in lettuce were explored under different conditions by using hydroponics. The results showed that the higher exposure concentration of Cs+ could lead to a faster uptake rate and would be beneficial to the uptake and accumulation of Cs+. The uptake of K+ by roots and leaves was inhibited significantly when Cs+ concentration increased, but unapparent for Ca2+ and Mg2+. It was found that the higher K+ and Ca2+ concentration was, the higher inhibition was found for the uptake of Cs+ in root. The uptake of Cs+ leads the decrease of chlorophyll content and brought a negative effect on plant photosynthesis, consequently, a negative effect on lettuce morphology and obvious decrease of biomass and root length. The contents of glutathione (GSH), malondialdehyde (MDA), and root vitality were increasing during the growth following stress of high concentrations of Cs+, which caused stresses on the antioxidant system of lettuce. The enrichment coefficient for Cs+ in leaves was in the range of 8-217. Moreover, the transfer factor was in the range of 0.114-0.828, which suggested that the high Cs+ concentration could enhance the transfer of Cs+ from lettuce root to leaf. This study provides more information on the transfer of RCs from water to food chain, promoting the understanding of the potential risk of RCs. [ABSTRACT FROM AUTHOR]

In this work, we set out to provide an experimental setup, using Cs-131, with associated dosimetry for studying relative biological effectiveness (RBE) of Auger emitters. Cs-131 decays by 100% electron capture producing K- (9%) and L- (80%) Auger electrons with mean energies of 26 keV and 3.5 keV, respectively, plus ≈ 9.4 very low energy electrons (<0.5 keV) per decay. Cs-131 accumulates in the cells through the Na+/K+-ATPase. By this uptake mechanism and the alkali chemistry of Cs+, we argue for its intracellular homogeneous distribution. Cs-131 was added to the cell culture medium of HeLa and V79 Cells. The bio-kinetics of Cs-131 (uptake, release, intracellular distribution) was examined by measuring its intracellular activity concentration over time. Taking advantage of the 100% confluent cellular monolayer, we developed a new and robust dosimetry that is entrusted to a quantity called SC-value. The SC-values evaluated in the cell nucleus are almost independent of the nuclear size and geometry. We obtained dose-rate controlled RBE-values for intracellular Cs-131 decay. Using the γH2AX assay, the RBE was 1 for HeLa cells. Using the clonogenic cell survival, it was 3.9 for HeLa cells and 3.2 for V79 cells. This experimental setup and dosimetry provides reliable RBE-values for Auger emitters in various cell lines. [ABSTRACT FROM AUTHOR]

Cystoseira sensu lato marine forests, which are among the most productive and diverse systems in rocky intertidal and subtidal habitats of the Mediterranean Sea, are experiencing a widespread decline throughout the basin due to increasing human pressures. Yet it is still unclear whether Marine Protected Areas (MPAs) may represent effective tools for conservation of these important habitat formers and their associated assemblages. Here, we compared the biomass of intertidal stands of Ericaria amentacea (C. Agardh) Molinari and Guiry and their understory assemblages between the no-take zone and control sites in two Mediterranean MPAs. We did not find evidence supporting a significant effect of full protection in increasing the biomass of E. amentacea stands and associated assemblages, except for macroalgae of the understory in one of the investigated MPAs, raising concerns on the potential effectiveness of MPAs in mitigating human impacts on these marine forests. Such findings call for major efforts to implement long-term monitoring programs of protected Cystoseira s.l. forests in order to inform an adaptive management of conservation measures within MPAs and eventually to set active interventions of restoration. [ABSTRACT FROM AUTHOR]

Improving the effectiveness of marine spatial management is crucial to preserve marine ecosystems and also to support the fisheries economy. In this study, we aimed to determine the spatial distribution and suitable habitats (spawning and nursery sites) of Sciaena umbra and Dentex dentex using catches data in Corsica Island. Random forest models have been used as they are highly accurate and are suited to predict rare species distribution. Predictive maps of spatial distribution based on presence/absence data showed a high degree of spatial variability between species. Suitable habitats were close to shore in shallow waters for S. umbra and deeper and further offshore for D. dentex. We also observed that spawning sites were mainly located on the west coast, while nursery areas were distributed all around the island for D. dentex. In addition, we demonstrated the important contribution of environmental factors and local habitats (depth and Posidonia oceanica meadows) in the selection of their suitable habitats. Our results provide new knowledge for understanding ecological processes such as the selection of suitable habitats by fish species. Based on this information, spatial mapping can be implemented to improve the effectiveness of resource management over the long term. [ABSTRACT FROM AUTHOR]

These results match with the condition of I G. barbata i thalli during the extremely warm temperatures in the S'cuza lagoon, where temperatures can exceed 30 °C and reach up to 34 °C up to several days. In this study considerable differences were found between the main morphological features of the two forms of I G. barbata i thalli compared with I G. barbata i thalli at the same depth from the central part of the Istrian Coast ([28]). Graph: Figure 2: Thallus of Gongolaria barbata: (A) Attached thallus on a pebble; (B) detached thallus with the holdfast; (C) detached thallus without a holdfast. Keywords: attached form; detached form; Gongolaria barbata; lagoon; northern Adriatic EN attached form detached form Gongolaria barbata lagoon northern Adriatic 255 268 14 08/09/22 20220801 NES 220801 1 Introduction Fucalean species in the Mediterranean Sea are mainly represented by members of the genera I Cystoseira i , I Ericaria i and I Gongolaria i ([2]; [3]; [4]; [24]; [25]; [34]; [51]). [Extracted from the article]

Rationale: The first‐row transition metals Cu, Ni, and Co show a strong binding affinity to natural organic matter. Compared to dissolved elements and stable water isotopes, they may be transported rapidly through the soil and host rock into caves in response to infiltration events. This study aims to assess the potential of transition metal ratios as indicators for infiltration changes in response to the seasonal and/or event‐based rainfall variation. Methods: We developed a protocol to analyze Cu, Ni, and Co in the cave drip water using collision cell ICP‐QMS without extensive sample pretreatment. The high Ca matrix leads to significant isobaric interferences on all isotope masses. Our method includes a correction of these matrix effects and yields results with comparable accuracy and reproducibility to other published methods. We applied this protocol to drip water samples from Larga Cave (Puerto Rico) covering at least two full annual cycles between 2014 and 2019 on a bimonthly scale. Results: The analysis of external reference materials yielded a reproducibility between 4.7% and 9.2% (relative standard deviation), validating the accuracy of the matrix correction method. The limit of detection is <0.04 ppb for Cu, <0.02 ppb for Ni, and <0.008 ppb for Co. The analysis of drip water samples from Larga Cave reveals pronounced changes of several orders of magnitude in all Element (El) to Ca, Cu/Ni, and Cu/Co ratios in response to seasonal infiltration changes. In addition, we observe a partly even stronger response after major tropical storms and heavy precipitation events of the period of record, for example, tropical storm "Bertha" (2014) and the category 5 hurricanes "Irma" and "Maria" (both 2017). Conclusions: Transition metal ratios can be accurately measured in cave drip waters with high Ca matrix. At our tropical site, these are promising tracers of infiltration changes in response to changes in the amount of rainfall, providing the first step toward tropical cyclone reconstruction using trace elements in speleothems. [ABSTRACT FROM AUTHOR]

Hybrid optical elements, which combine refractive and diffractive optical components to enhance optical performance by taking advantage of the optical characteristics of the individual components, have enormous potential for next-generation optical devices. However, there have not been many reports on the simulation methodology to characterize such hybrid optical systems. Here, we present a method for simulating a hybrid optical element realized by attaching an ultra-thin, flexible diffractive optics array onto a refractive optical element. The ultra-thin diffractive optical element is fabricated by direct-laser-writing using a femtosecond pulsed laser as the light source. A systematic investigation of the proposed simulation method, which does not require extensive hardware resources or computational time, but retains resolution and accuracy, is presented. The proposed scheme is validated by comparing simulation and experimental results. The simulation and experimental results on the spot size and focal length for the diffractive Fresnel zone plate (FZP) match well, with typical errors of less than 6%. The aspect ratio of the focal spot sizes at the compound and FZP focal planes of the hybrid optical system from the simulation and experiment also match quite well, with typical errors below 7%. This simulation scheme will expedite the designs for novel hybrid optical systems with optimal optical performances for specific applications, such as microfluidics and aberration-controlled optics. [ABSTRACT FROM AUTHOR]

The terrestrial and marine Port-Cros National (PCNP) was established in 1963; it was then made up only of the Archipelago of Port-Cros. Since 2012, it has been extended to include a vast land and sea area, including not only islands but also part of the mainland, the new PCNP (N-PCNP); the marine core area and the adjacent marine area cover approximately 120,000 ha and extend over 63 km as the crow flies, from east to west. Taxon richness is just one descriptor of biodiversity among others (e.g., functional and ecosystem diversity), and is far from being the most reliable one; however, it deserves to be taken into consideration, provided that certain prerequisites are met, because it constitutes a convenient measure of, e.g., the research effort and the diversity of habitats. The number of reported macrophyte taxa amounts to 502: 73 green algae, 316 red algae, 104 brown algae and 9 magnoliophyta and other taxa. Two new combinations are proposed: Ericaria brachycarpa var. claudiae and Gongolaria montagnei var. compressa. This gamma species diversity is far from being exceptionally high, but rather is within the norm for the Mediterranean, if we take into account the size of the area considered. The number of reported taxa per site is highly heterogeneous throughout the N-PCNP area; it is, as expected, correlated with the number of studies per site. The research effort peaked in the 1970–1980s, and then irregularly declined, which may seem surprising in this era of biodiversity launched at the 1992 Rio Summit. The exceptionally extensive database available, covering more than a century, provides the basis for a critical analysis of the concept of biodiversity, as proclaimed by the general public and the 'greens', which can be naive or biased, and of the concept of 'heritage value species', which the authors of this article consider to be a 'toxic concept', as opposed to 'ordinary biodiversity', which enables ecosystem functioning. However, this database, straddling both areas highly impacted by humans (coastal development, tourist resorts) and areas that are effectively protected, does not highlight obvious changes over time. [ABSTRACT FROM AUTHOR]

We report on the fabrication and characterization of high-resolution gratings with high efficiency in the extreme ultraviolet (EUV) and soft x-ray ranges using spin-on-carbon (SOC) underlayers. We demonstrate the fabrication of diffraction gratings down to 20 nm half-pitch (HP) on Si3N4 membranes with a bilayer of hydrogen silsesquioxane (HSQ) and spin-on-carbon and show their performance as a grating mask for extreme ultraviolet interference lithography (EUV-IL). High-resolution patterning of HSQ is possible only for thin films due to pattern collapse. The combination of this high-resolution resist with SOC circumvents this problem and enables the fabrication of high aspect ratio nanostructures. Rigorous coupled-wave analysis shows that the bilayer gratings exhibit higher diffraction efficiency than what is feasible with a grating made of HSQ. We also demonstrate a simple and accurate method to experimentally measure the diffraction efficiency of high-resolution gratings by measuring the relative ratio of the dose-to-clear curves of the photoresist. The measured diffraction efficiencies are in good agreement with the theoretically predicted values. Furthermore, we verify our calculations and measurements by printing line/space patterns in chemically amplified resists down to 10 nm HP with both HSQ and bilayer grating masks using EUV-IL. The improved diffraction efficiency of the bilayers is expected to have applications not only in gratings for interference lithography, but also in Fresnel zone plates and gratings for spectroscopy in the EUV and soft x-ray ranges. [ABSTRACT FROM AUTHOR]

One of the greatest challenges in the development of binaural machine audition systems is the disambiguation between front and back audio sources, particularly in complex spatial audio scenes. The goal of this work was to develop a method for discriminating between front and back located ensembles in binaural recordings of music. To this end, 22, 496 binaural excerpts, representing either front or back located ensembles, were synthesized by convolving multi-track music recordings with 74 sets of head-related transfer functions (HRTF). The discrimination method was developed based on the traditional approach, involving hand-engineering of features, as well as using a deep learning technique incorporating the convolutional neural network (CNN). According to the results obtained under HRTF-dependent test conditions, CNN showed a very high discrimination accuracy (99.4%), slightly outperforming the traditional method. However, under the HRTF-independent test scenario, CNN performed worse than the traditional algorithm, highlighting the importance of testing the algorithms under HRTF-independent conditions and indicating that the traditional method might be more generalizable than CNN. A minimum of 20 HRTFs are required to achieve a satisfactory generalization performance for the traditional algorithm and 30 HRTFs for CNN. The minimum duration of audio excerpts required by both the traditional and CNN-based methods was assessed as 3 s. Feature importance analysis, based on a gradient attribution mapping technique, revealed that for both the traditional and the deep learning methods, a frequency band between 5 and 6 kHz is particularly important in terms of the discrimination between front and back ensemble locations. Linear-frequency cepstral coefficients, interaural level differences, and audio bandwidth were identified as the key descriptors facilitating the discrimination process using the traditional approach. [ABSTRACT FROM AUTHOR]

Oral malignant melanoma (OMM) is the most common neoplasm of the canine oral cavity. It is characterized by its aggressive local disease as well as its high rate of lymphatic invasion and distant metastasis. OMM carries a poor prognosis, with most patients succumbing to the disease due to progression of the neoplasm. Histopathologically, OMM is characterized by significant nuclear atypia, a mitotic index of greater than 4/10 hpf, and evidence of vascular invasion or metastasis. Clinically, these lesions can become locally invasive, causing lysis of bones and severe inflammation of the surrounding soft tissue. With time, these lesions can spread to the regional lymph node and to the lungs and other organs. Prognosis can vary depending on the size of the primary tumor, regional node involvement, and distant metastatic disease; however, multiple studies report a relatively short median survival time ranging from less than 4 months to 8 months. Histologically well- differentiated melanocytic neoplasms (HWDMN) are a variant of OMM and sometimes referred to as canine oral melanocytic neoplasms of low malignant potential. Unlike OMM, patients with HWDMN have longer survival times. Histopathologically, HWDMNs have well-differentiated melanocytes with a low mitotic index of 3 or less per 10 hpf and minimal nuclear atypia. HWDMNs have better prognosis with a mean survival time of up to 34 months. This article is a comparative review of OMM and its less aggressive counterpart. [ABSTRACT FROM AUTHOR]

Photosynthetic features and dark respiration of different species of dominant canopy‐forming algae of the Order Fucales (Phaeophyceae) consistently change with depth. Photosynthetic efficiency (α) and light at compensation (Ic) change according to the amount of light available at each depth, α increasing and Ic decreasing with depth. However, the values of light at saturation (Ik) do not show any depth pattern. Deep‐water species show higher photosynthesis at saturation (Pmax) and lower dark respiration (Rd) than shallow water ones. This result contrasts with the expected reduction of Pmax in low light environments shown by terrestrial plants but is consistent with other studies performed in macroalgae, suggesting that Pmax and α show the same trend of variation in the marine environment. The positive correlation between Pmax and α cannot be explained by differences in thickness as all tested species show an extremely high morphological similarity. The increase in Pmax with depth is also coupled to an increase of N and P content in branches, which points to nutrient‐limited photosynthesis in shallow water species. [ABSTRACT FROM AUTHOR]

Numerous advances have been made in X-ray technology in recent years. X-ray imaging plays an important role in the nondestructive exploration of the internal structures of objects. However, the contrast of X-ray absorption images remains low, especially for materials with low atomic numbers, such as biological samples. X-ray phase-contrast images have an intrinsically higher contrast than absorption images. In this review, the principles, milestones, and recent progress of X-ray phase-contrast imaging methods are demonstrated. In addition, prospective applications are presented. [ABSTRACT FROM AUTHOR]

Algal diversity in aquatic environments can help assess the health status of ecosystems, provide information about invasions of new species and inform species diversity changes according to environmental conditions. An algal diversity survey (2016–19) of Nador Lagoon (northern Morocco) revealed the presence for the first time of the brown alga Cystoseira aurantia. Since 2015 this alga has been found in high abundance in the northwestern part of the lagoon, mainly around Attalayoun and Nador City. The thalli of larger specimens reached 60 cm in length, and all plants present were free-floating. The species was collected from the water surface to depths not exceeding 2 cm. Floating patches were usually found suspended above sandy and muddy substrates. Increased abundance of this species in the lagoon corresponds with new physico-chemical conditions and ecological parameters, principally resulting from increased water ventilation and improved water quality since 2012. The new communities of C. aurantia may offer a novel resource for the lagoon but high abundance could pose future threats to attached vegetation such as seagrasses and benthic algae by shading and the accumulation of organic detritus. Attention is drawn to taxonomic issues involving C. aurantia. [ABSTRACT FROM AUTHOR]

Musical performance is a multimodal experience, for performers and listeners alike. This paper reports on a pilot study which constitutes the first step toward a comprehensive approach to the experience of music as performed. We aim at bridging the gap between qualitative and quantitative approaches, by combining methods for data collection. The purpose is to build a data corpus containing multimodal measures linked to high-level subjective observations. This will allow for a systematic inclusion of the knowledge of music professionals in an analytic framework, which synthesizes methods across established research disciplines. We outline the methods we are currently developing for the creation of a multimodal data corpus dedicated to the analysis and exploration of instrumental music performance from the perspective of embodied music cognition. This will enable the study of the multiple facets of instrumental music performance in great detail, as well as lead to the development of music creation techniques that take advantage of the cross-modal relationships and higher-level qualities emerging from the analysis of this multi-layered, multimodal corpus. The results of the pilot project suggest that qualitative analysis through stimulated recall is an efficient method for generating higher-level understandings of musical performance. Furthermore, the results indicate several directions for further development, regarding observational movement analysis, and computational analysis of coarticulation, chunking, and movement qualities in musical performance. We argue that the development of methods for combining qualitative and quantitative data are required to fully understand expressive musical performance, especially in a broader scenario in which arts, humanities, and science are increasingly entangled. The future work in the project will therefore entail an increasingly multimodal analysis, aiming to become as holistic as is music in performance. [ABSTRACT FROM AUTHOR]

Aims: We tested if chicory acquires nutrients from soil layers down to 3.5 m depth and whether the deep nutrient uptake increases as a result of drought or intercropping with ryegrass and black medic. We also tested whether application of the trace elements Cs, Li, Rb, Sr and Se, as tracers, result in similar uptake rates. Methods: The methodological tests were carried out in a pilot experiment where the tracers were applied to 1 m depth in lucerne and red beet grown in tube rhizotrons. The dynamics of deep nutrient uptake in chicory was studied in large 4 m deep rhizoboxes. A drought was induced when roots had reached around 2 m depth. Results: Chicory acquired 15N from 3.5 m depth and trace element tracers from 2.3 m depth. We found no compensatory tracer uptake with depth during drought. We found some indications of a compensatory tracer uptake from 2.3 and 2.9 m depth in intercropped chicory. Application of equimolar amounts of trace elements resulted in similar excess tracer concentrations within species. Conclusion: Chicory demonstrates nutrient uptake from below 3 m but does not increase deep nutrient uptake as a response to limited topsoil nutrient availability induced by drought or intercropping. [ABSTRACT FROM AUTHOR]

Somatostatin (SST) is a cyclic peptide that is understood to inhibit the release of hormones and neurotransmitters from a variety of cells by binding to one of five canonical G protein-coupled SST receptors (SSTR1 to SSTR5). Recently, SST was also observed to interact with the amyloid beta (Aβ) peptide and affect its aggregation kinetics, raising the possibility that it may bind other brain proteins. Here we report on an SST interactome analysis that made use of human brain extracts as biological source material and incorporated advanced mass spectrometry workflows for the relative quantitation of SST binding proteins. The analysis revealed SST to predominantly bind several members of the P-type family of ATPases. Subsequent validation experiments confirmed an interaction between SST and the sodium-potassium pump (Na+/K+-ATPase) and identified a tryptophan residue within SST as critical for binding. Functional analyses in three different cell lines indicated that SST might negatively modulate the K+ uptake rate of the Na+/K+-ATPase. [ABSTRACT FROM AUTHOR]

The aim of the study was to develop a method for automatic classification of the three spatial audio scenes, differing in horizontal distribution of foreground and background audio content around a listener in binaurally rendered recordings of music. For the purpose of the study, audio recordings were synthesized using thirteen sets of binaural-room-impulse-responses (BRIRs), representing room acoustics of both semi-anechoic and reverberant venues. Head movements were not considered in the study. The proposed method was assumption-free with regards to the number and characteristics of the audio sources. A least absolute shrinkage and selection operator was employed as a classifier. According to the results, it is possible to automatically identify the spatial scenes using a combination of binaural and spectro-temporal features. The method exhibits a satisfactory classification accuracy when it is trained and then tested on different stimuli but synthesized using the same BRIRs (accuracy ranging from 74% to 98%), even in highly reverberant conditions. However, the generalizability of the method needs to be further improved. This study demonstrates that in addition to the binaural cues, the Mel-frequency cepstral coefficients constitute an important carrier of spatial information, imperative for the classification of spatial audio scenes. [ABSTRACT FROM AUTHOR]

Marine Protected Areas (MPAs) are a major tool to conserve marine ecosystems but are also strongly attractive to tourists, the increased numbers of which can cause environmental issues if not properly managed.Proposing an MPA and then failing to establish it risks advertising the beauty of a marine area without managing the unavoidable increase in tourism. This is what happened at Gallinara Island (Ligurian Sea, north‐western Mediterranean), where an MPA was proposed in 1990 but has not yet been established.Benthic data collected soon after the proposal (1990–92) have been compared with similar information obtained in subsequent periods (2009 and 2016): change has been assessed both visually and statistically, using multivariate and univariate techniques.The Gallinara sea floor supported five epibenthic communities in 1991, four of which corresponded to European Nature Information System (EUNIS) habitats; the fifth epibenthic community, characterized by the erect sponge Axinella polypoides, should be included in the next revision of EUNIS.In the 25 years since the original MPA proposal, all epibenthic communities have exhibited a reduction in their three‐dimensional structure and biotic homogenization, and severe decreases in species diversity, and the sea floor has been littered with derelict fishing gear and other waste from boats.In absence of the MPA, proposals to develop a management plan for a marine Site of Community Importance (SCI) have been provided, to reduce sea‐based human pressures. The dramatic alteration of Gallinara sea floor after 25 years of negligence and inaction is a sad warning for other unmanaged yet beautiful natural areas within urbanized regions of the world. [ABSTRACT FROM AUTHOR]

Due to multiple impacts, Cystoseira forests are experiencing a significant decline, which is affecting the ecosystem services they provide. Despite conservation efforts, there is an urgent need to develop best practices and large-scale restoration strategies. To implement restoration actions, we developed an ex situ protocol for the cultivation of Cystoseira. amentacea var. stricta, aimed at reducing the time needed for laboratory culture, thus avoiding prolonged maintenance and minimizing costs. Specifically, we tested the effects of temperature, light and substratum on settlement and growth of early life stages using a factorial experiment. Temperature (20 and 24°C) and photoperiod (15L:9D) were selected to reflect the conditions experienced in the field during the reproductive period. Two light intensities (125 and 250 μmol photons m−2s−1) were selected to mimic the condition experienced in the absence of canopy (i.e. barren—higher light intensity) or in the understory (lower light intensity) during gamete release. The tested substrata were flat polished pebbles and rough clay tiles. The release of gametes and the successive survival and development of embryo and germlings were followed for two weeks. Regardless of the culture conditions, rougher tiles showed higher zygote settlement, but the substrata did not affect the successive development. Zygote mortality after one week averaged 50% and at the end of the second week, embryonic survival was higher under lower light and temperature conditions, which also determined the growth of larger embryos. [ABSTRACT FROM AUTHOR]

The current status of the TwinMic beamline at Elettra synchrotron light source, that hosts the European twin X-ray microscopy station, is reported. The X-ray source, provided by a short hybrid undulator with source size and divergence intermediate between bending magnets and conventional undulators, is energy-tailored using a collimated plane-grating monochromator. The TwinMic spectromicroscopy experimental station combines scanning and full-field imaging in a single instrument, with contrast modes such as absorption, differential phase, interference and darkfield. The implementation of coherent diffractive imaging modalities and ptychography is ongoing. Typically, scanning transmission X-ray microscopy images are simultaneously collected in transmission and differential phase contrast and can be complemented by chemical and elemental analysis using across-absorption-edge imaging, X-ray absorption near-edge structure or low-energy X-ray fluorescence. The lateral resolutions depend on the particular imaging and contrast mode chosen. The TwinMic range of applications covers diverse research fields such as biology, biochemistry, medicine, pharmacology, environment, geochemistry, food, agriculture and materials science. They will be illustrated in the paper with representative results. [ABSTRACT FROM AUTHOR]

Sensing and mapping element distributions in plant tissues and its growth environment has great significance for understanding the uptake, transport, and accumulation of nutrients and harmful elements in plants, as well as for understanding interactions between plants and the environment. In this study, we developed a 3-dimensional elemental mapping system based on laser-induced breakdown spectroscopy that can be deployed in- field to directly measure the distribution of multiple elements in living plants as well as in the soil. Mapping is performed by a fast scanning laser, which ablates a micro volume of a sample to form a plasma. The presence and concentration of specific elements are calculated using the atomic, ionic, and molecular spectral characteristics of the plasma emission spectra. Furthermore, we mapped the pesticide residues in maize leaves after spraying to demonstrate the capacity of this method for trace elemental mapping. We also used the system to quantitatively detect the element concentrations in soil, which can be used to further understand the element transport between plants and soil. We demonstrate that this method has great potential for elemental mapping in plant tissues and soil with the advantages of 3-dimensional and multi-elemental mapping, in situ and in vivo measurement, flexible use, and low cost. [ABSTRACT FROM AUTHOR]

The present study evaluated the impact of cesium (Cs) at four concentrations (0, 0.001, 0.01, and 0.1 mg L) on growth, concentrations of chlorophyll and carotenoid pigments, and oxidative stress responses in the charophyte, Nitella pseudoflabellata, over 30 days. Oxidative stress was quantified by measuring anti-oxidant enzyme activities and HO content. When compared with the control, significantly elevated activity levels of the anti-oxidative enzymes ascorbic peroxidase, catalase and guaiacol peroxidase were observed at 0.1 mg L (all p < 0.05), even though the HO level was not significantly elevated. Carotenoid and chlorophyll a and b pigment levels were significantly reduced (all p < 0.05) at Cs exposures of 0.01 and 0.1 mg L. Photosynthetic efficiency (i.e., F/F) was significantly reduced ( p < 0.05) at Cs concentrations ≥0.001 mg L. Significant reduction ( p < 0.05) of plant growth (i.e., shoot length) was also observed after 1 week of exposure at Cs concentrations ≥0.001 mg L. Our results suggested that Cs exposure reduced plant growth and affected plant functioning via activating the defense mechanism against oxidative stress in Nitella. [ABSTRACT FROM AUTHOR]