1. Analysis of the Basic Characteristics of Osteogenic and Chondrogenic Cell Lines Important for Tissue Engineering Implants.
- Author
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Astakhova NM, Korel' AV, Shchelkunova EI, Orishchenko KE, Nikolaev SV, Zubairova US, and Kirilova IA
- Subjects
- Alkaline Phosphatase genetics, Alkaline Phosphatase metabolism, Animals, Biomarkers metabolism, Bone and Bones metabolism, Calcium metabolism, Cartilage metabolism, Cell Differentiation, Cell Line, Cell Movement, Cell Size, Chondrocytes metabolism, Collagen Type II genetics, Collagen Type II metabolism, Gene Expression, Mitotic Index, Osteoblasts metabolism, Swine, Swine, Miniature, Bone and Bones cytology, Cartilage cytology, Chondrocytes cytology, Chondrogenesis genetics, Osteoblasts cytology, Osteogenesis genetics
- Abstract
We isolated and characterized cultures of bone and cartilage tissue cells of laboratory minipigs. The size and morphological features of adherent osteogenic and chondrogenic cells were specified. During long-term culturing under standard conditions, the studied cultures expressed specific markers that were detected by immunohistochemical staining: alkaline phosphatase and calcium deposits in osteoblasts and type II collagen and cartilage extracellular matrix in chondrogenic cells. Proliferative potential (mitotic index) of both cell types was 4.64% of the total cell number. Cell motility, i.e. the mean velocity of cell motion was 49 pixels/h for osteoblasts and 47 pixels/h for chondroblasts; the mean migration distance was 2045 and 2118 pixels for chondroblasts and osteoblasts, respectively. The obtained cell lines are now used as the control for evaluation of optimal biocompatibility of scaffold materials in various models. Characteristics of the motility of the bone and cartilage tissue cells can be used for modeling and estimation of the rate of cells population of 3D scaffolds made of synthetic and biological polymers with different internal structure and physicochemical properties during designing in vitro tissue implants.
- Published
- 2018
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