644 results on '"A. Hamid A. Hadi"'
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2. Effects of Covid-19 Infection on Some Pancreatic Functions in Diabetic Patients at Thi-Qar Province/Iraq
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Hamid S. Hadi and Sabah H. Enayah
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Diabetic, Covid-19, Pancreas ,Physics ,QC1-999 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
People with diabetes mellitus (DM) represent a population group that is at high risk of developing a poor prognosis for COVID-19.Having diabetes can lead to serious illness, ICU stay, and death from COVID-19.The current study included 150 participants with diabetes, 100 of whom had COVID-19 (46 males, 54 females), and 50 had diabetes only (28 males and 22 females), aged between 37 and 69 years. The current situation is in the isolation center for people infected with the Corona virus at Al-Hussein Teaching Hospital and Shatrah General Hospital in Thi-Qar Governorate.The study included the impact of COVID-19 infection and its effect on pancreatic function.The results of the current study recorded a significant increase in fasting blood glucose concentration and cumulative glucose concentration in diabetic patients infected with Covid-19 virus compared to diabetic patients only by gender, the results also indicated an increase in fasting glucose and cumulative glucose levels in both male and female COVID patients compared to only diabetic patients. The concentration of pancreatic hormones also increased significantly in both male and female patients infected with Covid-19 virus compared to diabetic patients. According to age groups and BMI, cumulative and fasting glucose concentrations did not register a significant difference according to age groups, while according to BMI, cumulative and fasting glucose concentrations increased in patients with normal weight. Trypsin concentration increased significantly in the first age group, while glucagon increased in the second age group. Trypsin and glucagon did not score a significant difference according to BMI
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- 2022
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3. Antimalarial ceramicines Q-T from Chisocheton ceramicus
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Alfarius Eko Nugroho, Chin Piow Wong, Yusuke Hirasawa, Toshio Kaneda, Takahiro Tougan, Toshihiro Horii, A. Hamid A. Hadi, and Hiroshi Morita
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Molecular Medicine - Published
- 2023
4. Modelling of Application-Centric IoT Solution for Guard Touring Communication Network
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Faizul, Amirul Hazeim, A. Rashid, Rozeha, Abdul Hamid, Abdul Hadi Fikri, Sarijari, Mohd Adib, Mohd, Alias, Abdullah, Ahmad Shahidan, Barbosa, Simone Diniz Junqueira, Series editor, Chen, Phoebe, Series editor, Filipe, Joaquim, Series editor, Kotenko, Igor, Series editor, Sivalingam, Krishna M., Series editor, Washio, Takashi, Series editor, Yuan, Junsong, Series editor, Zhou, Lizhu, Series editor, Mohamed Ali, Mohamed Sultan, editor, Wahid, Herman, editor, Mohd Subha, Nurul Adilla, editor, Sahlan, Shafishuhaza, editor, Md. Yunus, Mohd Amri, editor, and Wahap, Ahmad Ridhwan, editor
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- 2017
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5. Cycloartane triterpenoid (23R, 24E)-23-acetoxymangiferonic acid inhibited proliferation and migration in B16-F10 melanoma via MITF downregulation caused by inhibition of both β-catenin and c-Raf–MEK1–ERK signaling axis
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Kaneda, Toshio, Matsumoto, Misaki, Sotozono, Yayoi, Fukami, Satoshi, Nugroho, Alfarius Eko, Hirasawa, Yusuke, Hamid A, Hadi A., and Morita, Hiroshi
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- 2019
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6. Caloforines A–G, coumarins from the bark of Calophyllum scriblitifolium
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Ai Ogasawara, Ryo Noguchi, Takuya Shigi, Alfarius Eko Nugroho, Yusuke Hirasawa, Toshio Kaneda, Takahiro Tougan, Toshihiro Horii, A. Hamid A. Hadi, and Hiroshi Morita
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Molecular Medicine - Published
- 2022
7. Service-Oriented Architecture for IoT Home Area Networking in 5G
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Abd Rahim, Mohd Rozaini, primary, Rashid, Rozeha A., additional, Rateb, Ahmad M., additional, Sarijari, Mohd Adib, additional, Abdullah, Ahmad Shahidan, additional, Hamid, Abdul Hadi Fikri Abdul, additional, Sayuti, Hamdan, additional, and Fisal, Norsheila, additional
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- 2018
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8. Correction to: Cycloartane triterpenoid (23R, 24E)-23-acetoxymangiferonic acid inhibited proliferation and migration in B16-F10 melanoma via MITF downregulation caused by inhibition of both β-catenin and c-Raf–MEK1–ERK signaling axis
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Kaneda, Toshio, Matsumoto, Misaki, Sotozono, Yayoi, Fukami, Satoshi, Nugroho, Alfarius Eko, Hirasawa, Yusuke, Hamid A, Hadi A., and Morita, Hiroshi
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- 2020
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9. Development of TelG Mote for Wireless Biomedical Sensor Network (WBSN) Application
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A. Rahim, M. Rozaini, Rashid, Rozeha A., Ariffin, S. H. S., Fisal, N., Fikri A. Hamid, A. Hadi, Sarijari, M. Adib, Mohd, Alias, Abd Manaf, Azizah, editor, Sahibuddin, Shamsul, editor, Ahmad, Rabiah, editor, Mohd Daud, Salwani, editor, and El-Qawasmeh, Eyas, editor
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- 2011
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10. RETRACTED ARTICLE: Gastroprotective effect of desmosdumotin C isolated from Mitrella kentii against ethanol-induced gastric mucosal hemorrhage in rats: possible involvement of glutathione, heat-shock protein-70, sulfhydryl compounds, nitric oxide, and anti-Helicobacter pylori activity
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Heyam Mohamed Ali Sidahmed, Ainnul Hamidah Syahadah Azizan, Syam Mohan, Mahmood Ameen Abdulla, Siddig Ibrahim Abdelwahab, Manal Mohamed Elhassan Taha, A Hamid A Hadi, Kamal Aziz Ketuly, Najihah Mohd Hashim, Mun Fai Loke, and Jamuna Vadivelu
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Nitric Oxide ,Omeprazole ,Gastric Mucosa ,Gastric Ulcer ,Gastric Lesion ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Mitrella kentii (M. kentii) (Bl.) Miq, is a tree-climbing liana that belongs to the family Annonaceae. The plant is rich with isoquinoline alkaloids, terpenylated dihydrochalcones and benzoic acids and has been reported to possess anti-inflammatory activity. The purpose of this study is to assess the gastroprotective effects of desmosdumotin C (DES), a new isolated bioactive compound from M. kentii, on gastric ulcer models in rats. Methods DES was isolated from the bark of M. kentii. Experimental rats were orally pretreated with 5, 10 and 20 mg/kg of the isolated compound and were subsequently subjected to absolute ethanol-induced acute gastric ulcer. Gross evaluation, mucus content, gastric acidity and histological gastric lesions were assessed in vivo. The effects of DES on the anti-oxidant system, non-protein sulfhydryl (NP-SH) content, nitric oxide (NO)level, cyclooxygenase-2 (COX-2) enzyme activity, bcl-2-associated X (Bax) protein expression and Helicabacter pylori (H pylori) were also investigated. Results DES pre-treatment at the administered doses significantly attenuated ethanol-induced gastric ulcer; this was observed by decreased gastric ulcer area, reduced or absence of edema and leucocytes infiltration compared to the ulcer control group. It was found that DES maintained glutathione (GSH) level, decreased malondialdehyde (MDA) level, increased NP-SH content and NO level and inhibited COX-2 activity. The compound up regulated heat shock protein-70 (HSP-70) and down regulated Bax protein expression in the ulcerated tissue. DES showed interesting anti-H pylori effects. The efficacy of DES was accomplished safely without any signs of toxicity. Conclusions The current study reveals that DES demonstrated gastroprotective effects which could be attributed to its antioxidant effect, activation of HSP-70 protein, intervention with COX-2 inflammatory pathway and potent anti H pylori effect.
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- 2013
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11. Panduratin A, a Possible Inhibitor in Metastasized A549 Cells through Inhibition of NF-Kappa B Translocation and Chemoinvasion
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Mohd. Rais Mustafa, Sui-Ting Lee, Siew-Li Lai, Shiau-Chuen Cheah, and A. Hamid A. Hadi
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panduratin A ,NF-κB ,caspase ,MMP-2 ,p53 ,p21 ,Organic chemistry ,QD241-441 - Abstract
In the present study, we investigated the effects of panduratin A (PA), isolated from Boesenbergia rotunda, on apoptosis and chemoinvasion in A549 human non-small cell lung cancer cells. Activation of the executioner procaspase-3 by PA was found to be dose-dependent. Caspase-3 activity was significantly elevated at the 5 µg/mL level of PA treatment and progressed to a maximal level. However, no significant elevated level was detected on procaspase-8. These findings suggest that PA activated caspase-3 but not caspase-8. Numerous nuclei of PA treated A549 cells stained brightly by anti-cleaved PARP antibody through High Content Screening. This result further confirmed that PA induced apoptotic cell death was mediated through activation of caspase-3 and eventually led to PARP cleavage. Treatment of A549 cells with PA resulted in a strong inhibition of NF-κB activation, which was consistent with a decrease in nuclear levels of NF-κB/p65 and NF-κB/p50 and the elevation of p53 and p21. Besides that, we also showed that PA significantly inhibited the invasion of A549 cells in a dose-dependent manner through reducing the secretion of MMP-2 of A549 cells gelatin zymography assay. Our findings not only provide the effects of PA, but may also be important in the design of therapeutic protocols that involve targeting of either p53 or NF-κB.
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- 2013
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12. Aporphine Alkaloids from the Leaves of Phoebe grandis (Nees) Mer. (Lauraceae) and Their Cytotoxic and Antibacterial Activities
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A. Hamid A. Hadi, Ainnul Hamidah Syahadah Azizan, Siddiq Ibrahim Abdelwahab, Noraziah Nordin, Asdren Zajmi, Najihah Mohd. Hashim, Hanita Omar, and Hapipah Mohd Ali
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Phoebe grandis ,Lauraceae ,aporphine alkaloid ,cytotoxic ,antibacterial ,Organic chemistry ,QD241-441 - Abstract
The oxoaporphine alkaloid lysicamine (1), and three proaporphine alkaloids, litsericinone (2), 8,9,11,12-tetrahydromecambrine (3) and hexahydromecambrine A (4) were isolated from the leaves of Phoebe grandis (Nees) Merr. (Lauraceae). Compounds 2 and 3 were first time isolated as new naturally occurring compounds from plants. The NMR data for the compounds 2–4 have never been reported so far. Compounds 1 and 2 showed significant cytotoxic activity against a MCF7 (human estrogen receptor (ER+) positive breast cancer) cell line with IC50 values of 26 and 60 µg/mL, respectively. Furthermore, in vitro cytotoxic activity against HepG2 (human liver cancer) cell line was evaluated for compounds 1–4 with IC50 values of 27, 14, 81 and 20 µg/mL, respectively. Lysicamine (1) displayed strong antibacterial activity against Bacillus subtilis (B145), Staphylococcus aureus (S1434) and Staphylococus epidermidis (a clinically isolated strain) with inhibition zones of 15.50 ± 0.57, 13.33 ± 0.57 and 12.00 ± 0.00 mm, respectively. However, none of the tested pathogenic bacteria were susceptible towards compounds 2 and 3.
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- 2013
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13. Phenolic Compound, Triterpene and Steroids From The Leaves and Bark of Dysoxylum Macrocarpum
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Ibrahim A. Najmuldeen, Kamal Aziz Ketuly, and A. Hamid A. Hadi
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The ,Science - Abstract
The bark and leaves of Dysoxylum macrocarpum (Meliaceace) yielded four compounds, two compounds from the leaves; 5-hydroxy-7-methoxy-2-methyl-4H-chromen-4-one (Eugenin) DM1, which was new as crystal and squalene DM2, while two more compounds were found from the bark; stigmasterol DM3 and sitosterol DM4. The present work involves extraction, isolation and purification of compounds by using column chromatography followed by preparative TLC. Structural elucidation has been done through several spectroscopic methods, notably UV, IR, MS (HRMS, GCMS, and LCMS), 1D, 2D-NMR 1H NMR, 13CNMR, COSY, DEPT, HMQC, HMBC, and single crystal X-ray diffraction analysis.
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- 2013
14. Cholinesterase Enzymes Inhibitors from the Leaves of Rauvolfia Reflexa and Their Molecular Docking Study
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Vikneswaran Murugaiyah, Alireza Basiri, A. Hamid A. Hadi, Yalda Kia, and Mehran Fadaeinasab
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Apocynaceae ,Rauvolfia reflexa ,acetylcholinesterase ,butyrylcholinesterase ,molecular docking ,Organic chemistry ,QD241-441 - Abstract
Plants of the Apocynaceae family have been traditionally used in the treatment of age-related brain disorders. Rauvolfia reflexa, a member of the family, has been used as an antidote for poisons and to treat malaria. The dichloromethane, ethanol and methanol extracts from the leaves of Rauvolfia reflexa showed potential acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities, with IC50 values in the 8.49 to 52.23 g/mL range. Further cholinesterase inhibitory-guided isolation of these extracts afforded four bioactive compounds, namely: (E)-3-(3,4,5-trimethoxyphenyl)acrylic acid (1), (E)-methyl 3-(4-hydroxy-3,5-dimethoxyphenyl) acrylate (2), 17-methoxycarbonyl-14-heptadecaenyl-4-hydroxy-3-methoxycinnamate (3) and 1,2,3,4-tetrahydro-1-oxo-β-carboline (4). The isolated compounds showed moderate cholinesterase inhibitory activity compared to the reference standard, physostigmine. Compounds 1 and 2 showed the highest inhibitory activity against AChE (IC50 = 60.17 µM) and BChE (IC50 = 61.72 µM), respectively. Despite having similar molecular weight, compounds 1 and 2 were structurally different according to their chemical substitution patterns, leading to their different enzyme inhibition selectivity. Compound 2 was more selective against BChE, whereas compound 1 was a selective inhibitor of AChE. Molecular docking revealed that both compounds 1 and 2 were inserted, but not deeply into the active site of the cholinesterase enzymes.
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- 2013
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15. Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro: Involvement of HSP70 and Bcl2/Bax signalling pathways
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Norbaiti Mohd Isa, Ahmad Bustamam Abdul, Siddig Ibrahim Abdelwahab, Rasedee Abdullah, Mohd Aspollah Sukari, Behnam Kamalidehghan, A. Hamid A. Hadi, and Syam Mohan
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Boesenbergia rotunda ,Boesenbergin A ,Apoptosis ,Mitochondria ,HSP70 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The anti-cancer effect of Boesenbergin A (BA) isolated from Boesenbergia rotunda, via the induction of apoptosis resulting from mitochondrial dysfunction was assessed in human non-small cell lung cancer (A549) cells. The apoptotic mechanisms of BA induction on cancer cells were studied in the present study for the first time. Nuclear stain, measuring the accumulation of sub-G1 cell population and DNA ladder were done to determine the apoptosis. Further investigations into the depletion of mitochondrial membrane potential and release of cytochrome c determined that BA treatment induced apoptosis via the regulation of the expression of pro-survival and pro-apoptotic Bcl-2 family members. The involvement of both intrinsic and extrinsic caspases (caspase 3/7, 9 and 8) were significantly increased. Moreover the role of free radicals was significantly found to be elevated with concomitant decrease in HSP70. In conclusion the results from the current study indicated BA could be a promising agent for the treatment of lung cancer.
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- 2013
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16. Free Radical Scavenging, Antimicrobial and Immunomodulatory Activities of Orthosiphon stamineus
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Nabil S. Harmal, Hamid A. Hadi, Suhailah W. Qader, Mohammed A. Alshawsh, Salmah Ismail, Zahra A. Amin, and Mahmood A. Abdulla
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antibacterial ,minimum inhibitory concentration ,peripheral blood mononuclear cells (PBMCs) ,DPPH ,Organic chemistry ,QD241-441 - Abstract
Orthosiphon stamineus is considered an important traditional folk medicine. In this study ethanol and aqueous extracts of O. stamineus were evaluated in vitro for their antioxidant, antimicrobial as well as for their immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). The DPPH radical scavenging method was used for the determination of antioxidant activity, while the antibacterial efficacy was investigated by both disc diffusion method and Minimum Inhibitory Concentration (MIC) against four bacterial strains (Gram-positive and Gram-negative). Furthermore, the immunomodulatory potential of the extracts was investigated through the MTT assay. Aqueous extract of O. stamineus exhibited significant free radical scavenging activity with IC50 9.6 µg/mL, whereas the IC50 for the ethanol extract was 21.4 µg/mL. The best antimicrobial activity was shown by the aqueous extract of O. stamineus against Staphylococcus aureus, with inhibition zone of 10.5 mm and MIC value 1.56 mg/mL. Moreover, the results observed from the MTT assay showed that both plant extracts stimulated the PBMCs proliferation in vitro in a concentration-dependent manner, but the aqueous extract has remarkable activity against PBMCs. These findings indicate that O. stamineus showed high antioxidant activity and may be considered as an immunomodulatory agent.
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- 2012
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17. Antibacterial Evaluation of Some Schiff Bases Derived from 2-Acetylpyridine and Their Metal Complexes
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Thong Kwai Lin, Chai Lay Ching, Cher Lin Ooi, A. Hamid A. Hadi, Mahmood Ameen Abdulla, Nura Suleiman Gwaram, Hapipah Mohd Ali, and Hamid Khaledi
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Schiff bases ,metal complexes ,x-ray crystallography ,anti-bacteria ,Organic chemistry ,QD241-441 - Abstract
A series of Schiff bases derived from 2-acetylpyridne and their metal complexes were characterized by elemental analysis, NMR, FT-IR and UV-Vis spectral studies. The complexes were screened for anti-bacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumanni (AC), Klebsiella pneumonie (KB) and Pseudomonas aeruginosa (PA) using the disc diffusion and micro broth dilution assays. Based on the overall results, the complexes showed the highest activities against MRSA while a weak antibacterial activity was observed against A. baumanii and P. aeruginosa.
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- 2012
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18. Ipomoea aquatica Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity
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A. Hamid A. Hadi, Siddig Ibrahim Abdelwahab, Suzy Munir Salama, Salim Said Alkiyumi, Mahmood Ameen Abdullah, and Ahmed Salim Alrashdi
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Ipomoea aquatica ,hepatoprotective ,thioacetamide ,oxidative stress ,Organic chemistry ,QD241-441 - Abstract
In the Indian system of traditional medicine (Ayurveda) it is recommended to consume Ipomoea aquatica to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of I. aquatica against liver damage were evaluated in thioacetamide (TAA)-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD) rats were orally fed with I. aquatica (250 and 500 mg/kg) for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months). The results showed that the treatment of I. aquatica significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time). The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by I. aquatica treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that I. aquatica has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of I. aquatica in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of I. aquatica for the treatment of liver disorders.
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- 2012
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19. (−)-Kunstleramide, a New Antioxidant and Cytotoxic Dienamide from the Bark of Beilschmiedia kunstleri Gamble
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A. Hamid A. Hadi, Abbas Mollataghi, and Shiau-Chuen Cheah
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Beilschmiedia kunstleri ,lauraceae ,alkaloid ,dienamide ,antioxidant ,cytotoxicity ,Organic chemistry ,QD241-441 - Abstract
A new dienamide, (2E,4E)-7-(3',4'-dimethoxyphenyl)-N-ethyl-6-(R)-hydroxyhepta- 2,4-dienamide, named (-)-kunstleramide (1), were isolated from the bark of Beilschmiedia kunstleri Gamble together with one neolignan: (+)-kunstlerone (2) and seven known alkaloids: (+)-nornuciferine (3), (-)-isocaryachine (4), (+)-cassythicine (5), (+)-laurotetanine (6), (+)-boldine (7), noratherosperminine (8), (+)-N-demethylphyllocaryptine (9). Their structures were established from spectroscopic techniques, most notably 1D- and 2D-NMR, UV, IR, OR, circular dichroism (CD) spectra and LCMS-IT-TOF. (-)-Kunstleramide (1) exhibited very poor dose-dependent inhibition of DPPH activity, with an IC50 value of 179.5 ± 4.4 μg/mL, but showed a moderate cytotoxic effect on MTT assays of A375, A549, HT-29, PC-3 and WRL-68 with EC50 values of 64.65, 44.74, 55.94, 73.87 and 70.95 µg/mL, respectively.
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- 2012
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20. Naucline, a New Indole Alkaloid from the Bark of Nauclea officinalis
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Marc Litaudon, Hiroshi Morita, Mohd Rais Mustafa, Kazumasa Zaima, Khalijah Awang, Sook Yee Liew, A. Hamid A. Hadi, and Mat Ropi Mukhtar
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naucline ,angustine ,angustidine ,nauclefine ,naucletine ,Rubiaceae ,vasorelaxant activity ,Organic chemistry ,QD241-441 - Abstract
A new indole alkaloid, naucline (1) together with four known alkaloids, angustine (2), angustidine (3), nauclefine (4) and naucletine (5), were isolated from the bark of Nauclea officinalis. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1D- and 2D- NMR, IR, UV and LCMS-IT-TOF. In addition to that of alkaloid 1, the complete 13C-NMR data of naucletine (5) were also reported. Naucline (1) showed a moderate vasorelaxant activity (90% relaxation at 1 × 10−5 M) whereas, angustine (2), nauclefine (4), and naucletine (5) showed potent vasorelaxant activity (more than 90% relaxation at 1 × 10−5 M) on an isolated rat aorta.
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- 2012
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21. Acute Toxicity Evaluation, Antibacterial, Antioxidant and Immunomodulatory Effects of Melastoma malabathricum
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A. Hamid A. Hadi, Mohammed A. Alshawsh, Zahra A. Amin Alnajar, Hapipah M. Ali, and Mahmood A. Abdulla
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Melastoma malabathricum ,antioxidant ,immunomodulatory ,antibacterial ,acute toxicity ,Organic chemistry ,QD241-441 - Abstract
Melastoma malabathricum (MM) is a well-known plant in Malaysian traditional medicine, locally known as senduduk. Its ethanol and aqueous extracts have been used in the present investigation to study the immunomodulatory role on human peripheral blood mononuclear cell (PBMC), and the DPPH, ABTS and FRAP free radical scavenging activities were also measured. Total flavonoids and total phenolic contents were assayed and the antibacterial effect was tested against four species of bacteria; two Gram-positive (Staphylococcus aureus and Streptococcus agalactiae) and two Gram-negative (Escherichia coli and Klebsilla pneumonia). The tests were carried out using the disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Moreover, the acute toxicity was evaluated in vivo on the ethanol extract of MM to establish its safety when administered orally. In our results, both extracts of MM showed abilities to scavenge DPPH and ABTS free radicals, IC50 values: (11.599 ± 0.84, 10.573 ± 0.58 µmol/L) and (62.657 ± 0.78, 63.939 ± 0.48 µmol/L) for ethanol and aqueous extracts respectively. Indeed the ethanol extract evidenced high phenolic content (384.33 ± 0.005 mg/g), flavonoids contents (85.8 ± 0.009 mg/g) and ferric reducing antioxidant power (33,590 ± 0.038 mmol/g), with high activity against S. aureus and S. agalactiae (11 ± 0.3 and 12 ± 0.6 mm inhibition zones). Likewise, the percentage of peripheral blood mononuclear cells (PBMC) viability was increased in response to MM, IC50 values (1.781 ± 1.2 and 6.545 ± 0.93 µg/mL) for ethanol and aqueous extracts, respectively. In addition, our results showed that the MM extract is safe even at a high dose of 5,000 mg/kg and has no oral toxicity. These findings suggest the excellent medicinal bioactivity of MM and explain the popularity of this plant in the folk medicine as a remedy for different illnesses.
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- 2012
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22. Synthesis, Characterization, X-ray Crystallography, Acetyl Cholinesterase Inhibition and Antioxidant Activities of Some Novel Ketone Derivatives of Gallic Hydrazide-Derived Schiff Bases
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Hamid Khaledi, Siddig Ibrahim Abdelwahab, Pouya Hassandarvish, A. Hamid A. Hadi, Abeer A. Alhadi, Wageeh A. Yehye, Rozana Othman, Lip Yong Chung, Sri Devi Sukumaran, Mahmood Ameen Abdulla, Nura Suleiman Gwaram, Michael J. C. Buckle, and Hapipah Mohd Ali
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gallic hydrazide Schiff bases ,AChE inhibition ,antioxidant study ,molecular docking ,Organic chemistry ,QD241-441 - Abstract
Alzheimer’s disease (AD) is the most common form of dementia among older people and the pathogenesis of this disease is associated with oxidative stress. Acetylcholinesterase inhibitors with antioxidant activities are considered potential treatments for AD. Some novel ketone derivatives of gallic hydrazide-derived Schiff bases were synthesized and examined for their antioxidant activities and in vitro and in silico acetyl cholinesterase inhibition. The compounds were characterized using spectroscopy and X-ray crystallography. The ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays revealed that all the compounds have strong antioxidant activities. N-(1-(5-bromo-2-hydroxyphenyl)-ethylidene)-3,4,5-trihydroxybenzohydrazide (2) was the most potent inhibitor of human acetyl cholinesterase, giving an inhibition rate of 77% at 100 μM. Molecular docking simulation of the ligand-enzyme complex suggested that the ligand may be positioned in the enzyme’s active-site gorge, interacting with residues in the peripheral anionic subsite (PAS) and acyl binding pocket (ABP). The current work warrants further preclinical studies to assess the potential for these novel compounds for the treatment of AD.
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- 2012
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23. Neonaucline, a New Indole Alkaloid from the Leaves of Ochreinauclea maingayii (Hook. f.) Ridsd. (Rubiaceae)
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Marc Litaudon, Hiroshi Morita, Kazuma Zaima, A. Hamid A. Hadi, Ahmad Kaleem Qureshi, Khalijah Awang, Hazrina Hazni, Mat Ropi Mukhtar, and Norfaizah Osman
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Ochreinauclea maingayii ,neonaucline ,nauledine ,cadamine ,Rubiaceae ,alkaloid ,Organic chemistry ,QD241-441 - Abstract
A new indole alkaloid; neonaucline (1), along with six known compounds–Cadamine (2), naucledine (3), harmane, benzamide, cinnamide and blumenol A–were isolated from the leaves of Ochreinauclea maingayii (Rubiaceae). In addition to that of compound 1, 13C-NMR data of cadamine (2) and naucledine (3) were also reported. Structural elucidations of these alkaloids were performed using spectroscopic methods especially 1D- and 2D-NMR, IR, UV and LCMS-IT-TOF. The excellent vasorelaxant activity on isolated rat aorta was observed for the alkaloids 1–3 after injection of each sample at 1 × 10−5 M.
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- 2011
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24. Synthesis, Characterization, Acetylcholinesterase Inhibition, Molecular Modeling and Antioxidant Activities of Some Novel Schiff Bases Derived from 1-(2-Ketoiminoethyl)piperazines
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A. Hamid A. Hadi, Sri Devi Sukumaran, Michael J. C. Buckle, Lam Kok Wai, Siddig I. Abdelwahab, Mahmood A. Abdullah, Hapipah M. Ali, and Saleh M. Salga
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1-(2-ketoiminoethyl)piperazines ,AChE inhibition ,molecular docking ,antioxidant study ,acute toxicity ,Organic chemistry ,QD241-441 - Abstract
Some novel Schiff bases derived from 1-(2-ketoiminoethyl)piperazines were synthesized and characterized by mass spectroscopy, FTIR, UV-Visible, 1H and 13C-NMR. The compounds were tested for inhibitory activities on human acetylcholinesterase (hAChE), antioxidant activities, acute oral toxicity and further studied by molecular modeling techniques. The study identified the compound (DHP) to have the highest activity among the series in hAChE inhibition and DPPH assay while the compound LP revealed the highest activity in the FRAP assay. The hAChE inhibitory activity of DHP is comparable with that of propidium, a known AChE inhibitor. This high activity of DHP was checked by molecular modeling which showed that DHP could not be considered as a bivalent ligand due to its incapability to occupy the esteratic site (ES) region of the 3D crystal structure of hAChE. The antioxidant study unveiled varying results in 1,1-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. This indicates mechanistic variations of the compounds in the two assays. The potential therapeutic applications and safety of these compounds were suggested for use as human acetylcholinesterase inhibitors and antioxidants.
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- 2011
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25. Mechanistic Studies of the Anti-Ulcerogenic Activity and Acute Toxicity Evaluation of Dichlorido-Copper(II)-4-(2-5-Bromo-benzylideneamino)ethyl) Piperazin-1-ium Phenolate Complex against Ethanol-Induced Gastric Injury in Rats
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A. Hamid A. Hadi, Hapipah Mohd Ali, Mahmood Ameen Abdullah, Siddig Ibrahim Abdelwahab, Pouya Davish Hussain, and Muhammad Saleh Salga
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Schiff bases ,acute toxicity ,anti-ulcer activity ,mechanism ,cytokines ,Organic chemistry ,QD241-441 - Abstract
The compound dichlorido-copper(II)-4-(2-5-bromobenzylideneamino)ethyl) piperazin-1-ium phenolate (CuLBS) was synthesized, characterized and screened for acute toxicity and protective activity against ethanol-induced gastric mucosal injury in rats. Gross microscopic lesions, biochemical and immunological parameters and histochemcial staining of glycogen storage were taken into consideration. Oral administration of CuLBS (30 and 60 mg/Kg) for two weeks dose-dependently flattened gastric mucosa, significantly increased gastric mucus and total acidity, compared with control group (P < 0.01). Serum levels of liver enzymes aspartate (AST) and alanine transaminases (ALT), pro-inflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines in the rats exposed to ethanol induced ulceration have been altered. Administration of CuLBS showed considerable (P < 0.05) protection against ulceration by modulating the acute alterations of cytokines AST, ALT and stomach glycogen. Interestingly, CuLBS did not interfere with the natural release of nitric oxide. CuLBS alone (60 mg/Kg) did not exhibit any ulcerogenic effect as assessed using Adami’s scoring scale. An acute toxicity study showed that rats treated with CuLBS (1,000 and 2,000 mg/Kg) manifested no abnormal signs. These findings therefore, suggested that the gastroprotective activity of CuLBS might contribute in modulating the inflammatory cytokine-mediated oxidative damage to gastric mucosa.
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- 2011
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26. (+)-Kunstlerone, a New Antioxidant Neolignan from the Leaves of Beilschmiedia kunstleri Gamble
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Marc Litaudon, Mat Ropi Mukhtar, Jamaludin Mohamad, Khalijah Awang, A Hamid A Hadi, and Abbas Mollataghi
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Beilschmiedia kunstleri ,alkaloid ,neolignan ,lauraceae ,antioxidant ,Organic chemistry ,QD241-441 - Abstract
A new neolignan, 3,4-dimethoxy-3′,4′-methylenedioxy-2,9-epoxy-6,7-cyclo-1,8-neolign-11-en-5(5H)-one, which has been named (+)-kunstlerone (1), together with six known alkaloids: (+)-norboldine (2), (+)-N-methylisococlaurine (3), (+)-cassythicine (4), (+)-laurotetanine (5), (+)-boldine (6) and (-)-pallidine (7), were isolated from the leaves of Beilschmiedia kunstleri. The structures were established through various spectroscopic methods notably 1D- and 2D-NMR, UV, IR and LCMS-IT-TOF. (+)- Kunstlerone (1) showed a strong antioxidant activity, with an SC50 of 20.0 µg/mL.
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- 2011
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27. BENZYLISOQUINOLINE ALKALOIDS FROM BARK OF Cryptocarya rugulosa
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Nurdin Saidi, Hiroshi Morita, Marc Litaudon, Mat Ropi Mukhtar, Khalijah Awang, and A. Hamid A. Hadi
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Cryptocarya rugulosa ,lauraceae ,benzylisoquinoline alkaloid ,Chemistry ,QD1-999 - Abstract
Seven known Benzylisoquinoline alkaloids, Papraline 1, (+)-norcinnamolaurine 2, (+)-codamine 3, (+)-6-methoxy-1-(3'-methoxybenzyl)-N-methyl-7-isoquinolinol 4, (+)-reticuline N-oxide 5, (+)-reticuline 6 and (-)-N-methylisococlaurine 7 were isolated from bark of Cryptocarya rugulosa. A combination of 1D-NMR (1H, 13C, DEPT, NOE), 2D-NMR (COSY, NOESY, HMQC, HMBC), MS (HRESI+, FAB+ and EI) spectroscopy used to elucidate the structure.
- Published
- 2011
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28. Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis
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Mohd Rais Mustafa, Dharmani Murugan, Lau Yeh-Siang, A. Hamid A. Hadi, and Gopal Subramaniam
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Phoebe grandis ,β-NADH ,isolated rat aorta ,endothelial dysfunction ,superoxide anions ,Organic chemistry ,QD241-441 - Abstract
Generation of reactive oxygen species plays a pivotal role in the development of cardiovascular diseases. The present study describes the effects of the methanolic extract of Phoebe grandis (MPG) stem bark on reactive oxygen species-induced endothelial dysfunction in vitro. Endothelium-dependent (acetylcholine, ACh) and -independent relaxation (sodium nitroprusside, SNP) was investigated from isolated rat aorta of Sprague-Dawley (SD) in the presence of the β-NADH (enzymatic superoxide inducer) and MPG extract. Superoxide anion production in aortic vessels was measured by lucigen chemiluminesence. Thirty minutes incubation of the rat aorta in vitro with β-NADH increased superoxide radical production and significantly inhibited ACh-induced relaxations. Pretreatment with MPG (0.5, 5 and 50 μg/mL) restored the ACh-induced relaxations (Rmax: 92.29% ± 2.93, 91.02% ± 4.54 and 88.31 ± 2.36, respectively) in the presence of β-NADH. MPG was ineffective in reversing the impaired ACh-induced relaxations caused by pyrogallol, a non-enzymatic superoxide generator. Superoxide dismutase (a superoxide scavenger), however, reversed the impaired ACh relaxations induced by both β-NADH and pyrogallol. MPG also markedly inhibited the β-NADH-induced generation of the superoxide radicals. Furthermore, MPG scavenging peroxyl radicals generated by tBuOOH (10−4 M).These results indicate that MPG may improve the endothelium dependent relaxations to ACh through its scavenging activity as well as by inhibiting the NADH/NADPH oxidase induced generation of superoxide anions.
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- 2011
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29. N-Cyanomethylnorboldine: A New Aporphine Isolated from Alseodaphne perakensis (Lauraceae)
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A. Hamid A. Hadi, Marc Litaudon, Khalijah Awang, Hiroshi Morita, Kartini Ahmad, Hanita Omar, Mat Ropi Mukhtar, and Mohd Azlan Nafiah
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Alseodaphne perakensis ,Lauraceae ,aporphine alkaloid ,Organic chemistry ,QD241-441 - Abstract
A phytochemical study of the bark of Alseodaphne perakensis has yielded three aporphine alkaloids: the new compound N-cyanomethylnorboldine (1), and the two known alkaloids N-methyllaurotetanine (2) and norboldine (3). The isolation was achieved by chromatographic techniques and the structural elucidation was performed via spectral methods, notably 1D- and 2D-NMR, UV, IR, and HRFABMS. The vasorelaxation activity of compound 1 has been studied.
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- 2011
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30. Lancifoliaine, a New Bisbenzylisoquinoline from the Bark of Litsea lancifolia
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Kazumasa Zaima, Marc Litaudon, Azeana Zahari, Hazrina Hazni, A Hamid A Hadi, Khalijah Awang, Mat Ropi Mukhtar, Syazreen Nadia Sulaiman, and Hiroshi Morita
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bisbenzylisoquinoline ,lancifoliaine ,N-allyllaurolitsine ,Lauraceae ,vaso-relaxant activity ,Organic chemistry ,QD241-441 - Abstract
A new bisbenzylisoquinoline, lancifoliaine (1), together with seven known alkaloids – N-allyllaurolitsine (2), reticuline (3), actinodaphnine, norboldine, pallidine, cassythicine and boldine – were isolated from the stem bark of Litsea lancifolia (Lauraceae). In addition to that of lancifoliaine, complete 13C-NMR data of N-allyl-laurolitsine (2) was also reported. The alkaloidal structures were elucidated by means of high field 1D- and 2D-NMR IR, UV, and LCMS-IT-TOF spectral data. N-Allyllaurolitsine (2) showed a moderate vasorelaxant activity on isolated rat aorta.
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- 2011
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31. Antifeedant Triterpenoids from the Seeds and Bark of Lansium domesticum cv Kokossan (Meliaceae)
- Author
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Roekmiati Tjokronegoro, Mat Ropi Mukhtar, A. Hamid A. Hadi, Khalijah Awang, Unang Supratman, and Tri Mayanti
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kokosanolides ,tetranortriterpenoid ,onoceranoid ,Lansium domesticum ,meliaceae ,antifeedant activity ,Organic chemistry ,QD241-441 - Abstract
Two tetranortriterpenoids, kokosanolide A (1) and C (2) were isolated from the seeds and three onoceranoid-type triterpenoids: kokosanolide B (3), 8,14-secogammacera-7,14-diene-3,21-dione (4) and a 1.5:0.5 mixture of 8,14-secogammacera-7,14(27)-diene-3,21-dione (5) and compound 4 were isolated from the bark of kokossan (Lansium domesticum). Complete 1H- and 13C-NMR data of the triterpenoids 1-5 are reported. The triterpenoids’ structures were elucidated primarily by means of high field 1D- and 2D-NMR, IR and HRMS spectral data. Triterpenoids 1-5 exhibited moderate to strong antifeedant activity against the fourth instar larvae of Epilachna vigintioctopunctata.
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- 2011
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32. Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation
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May-Lynn Lam, Mohd. Rais Mustafa, A. Hamid A. Hadi, David R. Appleton, Sui-Ting Lee, and Shiau-Chuen Cheah
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Panduratin A ,apoptosis ,High Content Screening ,Real-time Cellular Analyzer ,NF-κB ,Organic chemistry ,QD241-441 - Abstract
In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL (10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/ potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-κB inhibitor.
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- 2011
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33. APHORPINE ALKALOIDS FROM BARK OF Cryptocarya ferrea
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Nurdin Saidi, A. Hamid A. Hadi, Khalijah Awang, and Mat Ropi Mukhtar
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Chemistry ,QD1-999 - Abstract
Isolation, identification and characterization of the compounds isolated from the bark of Cryptocarya ferrea yielded three known aphorpine alkaloids. They are (-)-O-methylisopiline 1, (+)-norlirioferine 2 and (+)-lirioferine 3. Structural elucidation was established through several spectroscopic methods, such as 1D-NMR (1H, 13C, DEPT, NOE), 2D-NMR (COSY, NOESY, HMQC, HMBC), UV, IR, and MS and comparison with the published data. Keywords: Cryptocarya ferrea; (-)-o-methylisopiline, (+)-norlirioferine, (+)-lirioferine
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- 2010
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34. (6,7-Dimethoxy-4-methylisoquinolinyl)-(4’-methoxyphenyl)-methanone, a New Benzylisoquinoline Alkaloid from Beilschmiedia brevipes
- Author
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Pratiwi Pudjiastuti, Mat Ropi Mukhtar, A. Hamid A. Hadi, Nurdin Saidi, Hiroshi Morita, Marc Litaudon, and Khalijah Awang
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Beilschmiedia brevipes ,benzylisoquinoline ,lauraceae ,NMR ,Organic chemistry ,QD241-441 - Abstract
The leaves of Beilschmiedia brevipes provided a new benzylisoquinoline alkaloid: (6,7-dimethoxy-4-methylisoquinolinyl)-(4’-methoxyphenyl)-methanone (1) and O,O-dimethylannocherin A (2), a new natural compound which has been synthesized before. Complete 1H- and 13C-NMR data of both compounds were reported. The structures were established through various spectroscopic methods notably 1D- and 2D-NMR, UV, IR and HRESIMS.
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- 2010
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35. Boronate Derivatives of Functionally Diverse Catechols: Stability Studies
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Kamal Aziz Ketuly and A. Hamid A. Hadi
- Subjects
cyclic benzeneboronate ,catechols ,boronic acids ,Organic chemistry ,QD241-441 - Abstract
Benzeneboronate of catecholic carboxyl methyl esters, N-acetyldopamine, coumarin and catechol estrogens were prepared as crystalline derivatives in high yield. Related catechol compounds with extra polar functional group(s) (OH, NH2) do not form or only partially form unstable cyclic boronate derivatives.
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- 2010
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36. (+)-N-(2-Hydroxypropyl)lindcarpine: A New Cytotoxic Aporphine Isolated from Actinodaphne pruinosa Nees
- Author
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A. Hamid A. Hadi, Hanita Omar, Khalijah Awang, Marc Litaudon, Hiroshi Morita, Soleh Kosela, Muhammad Hanafi, Mat Ropi Mukhtar, Mohd Azlan Nafiah, and Tiah Rachmatiah
- Subjects
Actinodaphne pruinosa ,Lauraceae ,aporphine alkaloid ,cytotoxic ,Organic chemistry ,QD241-441 - Abstract
Onenewalkaloid; (+)-N-(2-hydroxypropyl)lindcarpine (1),together with four known aporphine alkaloids, (+)-boldine (2) (+)-norboldine (3), (+)-lindcarpine (4) and (+)-methyllindcarpine (5) were isolated from the stem bark of Actinodaphne pruinosa Nees (Lauraceae). (+)-N-(2-Hydroxypropyl)lindcarpine (1) exhibited cytotoxic activity against P-388 murine leukemia cells with an IC50 value of 3.9 μg/mL. Structural elucidation of all the compounds were performed by spectral methods such as 1D- and 2D- NMR, IR, UV, and HRESIMS.
- Published
- 2009
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37. Grandine A, a New Proaporphine Alkaloid from the Bark of Phoebe grandis
- Author
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Khalijah Awang, Marc Litaudon, Noel F. Thomas, A. Hamid A. Hadi, Ahmad Nazif Aziz, and Mat Ropi Mukhtar
- Subjects
Oxoproaporphine ,NMR ,Phoebe grandis ,Aporphine ,Dihydrolinearisine ,Organic chemistry ,QD241-441 - Abstract
The stem bark of Phoebe grandis afforded one new oxoproaporphine; (–)-grandine A (1), along with six known isoquinoline alkaloids: (–)-8,9-dihydrolinearisine (2), boldine, norboldine, lauformine, scortechiniine A and scortechiniine B. In addition to that of the new compound, complete 1H- and 13C-NMR data of the tetrahydroproaporphine (–)-8,9-dihydrolinearisine (2) is also reported. The alkaloids’ structures were elucidated primarily by means of high field 1D- and 2D-NMR and HRMS spectral data.
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- 2009
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38. Walsogynes H-O from Walsura chrysogyne
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Saori Nakajima, A. Hamid A. Hadi, Takahiro Tougan, Osamu Shirota, Toshihiro Horii, Hiroshi Morita, Yusuke Hirasawa, Alfarius Eko Nugroho, Toshio Kaneda, and Chin Piow Wong
- Subjects
Limonins ,Original Paper ,Magnetic Resonance Spectroscopy ,Molecular Structure ,Stereochemistry ,Chemistry ,Pharmacology toxicology ,Walsura chrysogyne ,Plasmodium falciparum ,Limonoid ,Antimalarial activity ,Antimalarials ,medicine ,Molecular Medicine ,Meliaceae ,Limonoids ,Two-dimensional nuclear magnetic resonance spectroscopy ,medicine.drug - Abstract
Graphic abstract Eight new limonoids, walsogynes H–O (1–8) were isolated from the barks of Walsura chrysogyne, and their structures were determined on the basis of the 1D and 2D NMR data. Walsogynes H–M (1–6) and O (8) were concluded to be 11,12-seco limonoids with a dodecahydro-1H-naphtho[1,8-bc:3,4-c′]difuran skeleton, and walsogyne N (7) to be 11,12-seco limonoid sharing a unique dodecahydronaphtho[1,8-bc:5,4-b′c′]difuran skeleton. Walsogynes H–O (1–8) exhibited potent antimalarial activity against Plasmodium falciparum 3D7 strain with IC50 value of 2.5, 2.6, 1.6, 2.5, 1.5, 2.6, 2.1, and 1.1 µM, respectively. Supplementary Information The online version contains supplementary material available at 10.1007/s11418-021-01556-4.
- Published
- 2021
39. Bioactive Natural Products
- Author
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A. Hamid A. Hadi, Mehmet Emin Duru, and Ana B. Martin-Diana
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Chemistry ,QD1-999 - Published
- 2013
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40. In vivo antioxidant and antiulcer activity of Parkia speciosa ethanolic leaf extract against ethanol-induced gastric ulcer in rats.
- Author
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Rami Al Batran, Fouad Al-Bayaty, Mazen M Jamil Al-Obaidi, Abdualrahman Mohammed Abdualkader, Hamid A Hadi, Hapipah Mohd Ali, and Mahmood Ameen Abdulla
- Subjects
Medicine ,Science - Abstract
BACKGROUND: The current study was carried out to examine the gastroprotective effects of Parkia speciosa against ethanol-induced gastric mucosa injury in rats. METHODOLOGY/PRINCIPAL FINDINGS: Sprague Dawley rats were separated into 7 groups. Groups 1-2 were orally challenged with carboxymethylcellulose (CMC); group 3 received 20 mg/kg omeprazole and groups 4-7 received 50, 100, 200 and 400 mg/kg of ethanolic leaf extract, respectively. After 1 h, CMC or absolute ethanol was given orally to groups 2-7. The rats were sacrificed after 1 h. Then, the injuries to the gastric mucosa were estimated through assessment of the gastric wall mucus, the gross appearance of ulcer areas, histology, immunohistochemistry and enzymatic assays. Group 2 exhibited significant mucosal injuries, with reduced gastric wall mucus and severe damage to the gastric mucosa, whereas reductions in mucosal injury were observed for groups 4-7. Groups 3-7 demonstrated a reversal in the decrease in Periodic acid-Schiff (PAS) staining induced by ethanol. No symptoms of toxicity or death were observed during the acute toxicity tests. CONCLUSION: Treatment with the extract led to the upregulation of heat-shock protein 70 (HSP70) and the downregulation of the pro-apoptotic protein BAX. Significant increases in the levels of the antioxidant defense enzymes glutathione (GSH) and superoxide dismutase (SOD) in the gastric mucosal homogenate were observed, whereas that of a lipid peroxidation marker (MDA) was significantly decreased. Significance was defined as p
- Published
- 2013
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41. Acute toxicity and gastroprotection studies with a newly synthesized steroid.
- Author
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Kamal A Ketuly, A Hamid A Hadi, Shahram Golbabapour, Maryam Hajrezaie, Pouya Hassandarvish, Hapipah Mohd Ali, Nazia Abdul Majid, and Mahmood A Abdulla
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Synthetic steroids, such as 9α-bromobeclomethasonedipropionate, have shown gastroprotective activity. For example, the potent glucocorticoid steroid, beclomethasone dipropionate, has been used for treatment of bowel ulcerations. The purpose of the present study was to evaluate the effect of a synthetic steroid, (20S)-22-acetoxymethyl-6β-methoxy-3α,5-dihydro-3'H-cyclopropa[3α,5]-5α-pregnane (AMDCP), on ethanol-induced gastric mucosa injuries in rats. METHODOLOGY/PRINCIPAL FINDING: Rats were divided into 8 groups. The negative control and ethanol control groups were administered Tween 20 (10%v/v) orally. The reference control group, 20 mg/kg omeprazole (10% Tween 20, 5 mL/kg), was administrated orally. The experimental groups received 1, 5, 10, 15 or 20 mg/kg of the AMDCP compound (10% Tween 20, 5 mL/kg). After 60 min, Tween 20 and absolute ethanol was given orally (5 mL/kg) to the negative control group and to the rest of the groups, and the rats were sacrificed an hour later. The acidity of gastric content, gastric wall mucus and areas of mucosal lesions were assessed. In addition, histology and immunohistochemistry of the gastric wall were assessed. Prostaglandin E2 (PGE2) and malondialdehyde (MDA) content were also measured. The ethanol control group exhibited severe mucosal lesion compared with the experimental groups with fewer mucosal lesions along with a reduction of edema and leukocyte infiltration. Immunohistochemical staining of Hsp70 and Bax proteins showed over-expression and under-expression, respectively, in the experimental groups. The experimental groups also exhibited high levels of PGE2 as well as a reduced amount of MDA. AMDCP decreased the acidity and lipid peroxidation and increased the levels of antioxidant enzymes. CONCLUSION/SIGNIFICANCE: The current investigation evaluated the gastroprotective effects of AMDCP on ethanol-induced gastric mucosal lesions in rats. This study also suggests that AMDCP might be useful as a gastroprotective agent.
- Published
- 2013
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42. Gastroprotection studies of Schiff base zinc (II) derivative complex against acute superficial hemorrhagic mucosal lesions in rats.
- Author
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Shahram Golbabapour, Nura Suleiman Gwaram, Pouya Hassandarvish, Maryam Hajrezaie, Behnam Kamalidehghan, Mahmood Ameen Abdulla, Hapipah Mohd Ali, A Hamid A Hadi, and Nazia Abdul Majid
- Subjects
Medicine ,Science - Abstract
BACKGROUND: The study was carried out to assess the gastroprotective effect of the zinc (II) complex against ethanol-induced acute hemorrhagic lesions in rats. METHODOLOGY/PRINCIPAL FINDING: The animals received their respective pre-treatments dissolved in tween 20 (5% v/v), orally. Ethanol (95% v/v) was orally administrated to induce superficial hemorrhagic mucosal lesions. Omeprazole (5.790×10(-5) M/kg) was used as a reference medicine. The pre-treatment with the zinc (II) complex (2.181×10(-5) and 4.362×10(-5) M/kg) protected the gastric mucosa similar to the reference control. They significantly increased the activity levels of nitric oxide, catalase, superoxide dismutase, glutathione and prostaglandin E2, and decreased the level of malondialdehyde. The histology assessments confirmed the protection through remarkable reduction of mucosal lesions and increased the production of gastric mucosa. Immunohistochemistry and western blot analysis indicated that the complex might induced Hsp70 up-regulation and Bax down-regulation. The complex moderately increased the gastroprotectiveness in fine fettle. The acute toxicity approved the non-toxic characteristic of the complex (
- Published
- 2013
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43. Acute toxicity and gastroprotection studies of a new schiff base derived copper (II) complex against ethanol-induced acute gastric lesions in rats.
- Author
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Maryam Hajrezaie, Shahram Golbabapour, Pouya Hassandarvish, Nura Suleiman Gwaram, A Hamid A Hadi, Hapipah Mohd Ali, Nazia Majid, and Mahmood Ameen Abdulla
- Subjects
Medicine ,Science - Abstract
BACKGROUND: Copper is an essential element in various metabolisms. The investigation was carried out to evaluate acute gastroprotective effects of the Copper (II) complex against ethanol-induced superficial hemorrhagic mucosal lesions in rats. METHODOLOGY/PRINCIPAL FINDINGS: Rats were divided into 7 groups. Groups 1 and 2 were orally administered with Tween 20 (10% v/v). Group 3 was orally administered with 20 mg/kg omeprazole (10% Tween 20). Groups 4-7 received 10, 20, 40, and 80 mg/kg of the complex (10% Tween 20), respectively. Tween 20 (10% v/v) was given orally to group 1 and absolute ethanol was given orally to groups 2-7, respectively. Rats were sacrificed after 1 h. Group 2 exhibited severe superficial hemorrhagic mucosal lesions. Gastric wall mucus was significantly preserved by the pre-treatment complex. The results showed a significant increase in glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), and Prostaglandin E2 (PGE(2)) activities and a decrease in malondialdehyde (MDA) level. Histology showed marked reduction of hemorrhagic mucosal lesions in groups 4-7. Immunohistochemical staining showed up-regulation of Hsp70 and down-regulation of Bax proteins. PAS staining of groups 4-7 showed intense stain uptake of gastric mucosa. The acute toxicity revealed the non-toxic nature of the compound. CONCLUSIONS/SIGNIFICANCE: The gastroprotective effect of the Copper (II) complex may possibly be due to preservation of gastric wall mucus; increase in PGE(2) synthesis; GSH, SOD, and NO up-regulation of Hsp70 protein; decrease in MDA level; and down-regulation of Bax protein.
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- 2012
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44. Bisleuconothines B–D, Modified Eburnane–Aspidosperma Bisindole Alkaloids from Leuconotis griffithii
- Author
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Chin Piow Wong, Wenjia Zhang, Hiroshi Morita, Yiming Tang, Yusuke Hirasawa, Alfarius Eko Nugroho, Toshio Kaneda, and A. Hamid A. Hadi
- Subjects
Aspidosperma ,Stereochemistry ,Monoterpene ,Dimer ,Pharmaceutical Science ,01 natural sciences ,Indole Alkaloids ,Analytical Chemistry ,Human lung ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Humans ,Pharmacology ,Molecular Structure ,biology ,Indole alkaloid ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,medicine.anatomical_structure ,Complementary and alternative medicine ,A549 Cells ,visual_art ,visual_art.visual_art_medium ,Molecular Medicine ,Bark ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Three new bisindole alkaloids, bisleuconothines B–D (1–3), were isolated from the bark of Leuconotis griffithii. Their structures were elucidated by 1D and 2D NMR spectroscopy and DFT calculations. Bisleuconothine B (1) is the first monoterpene indole alkaloid dimer featuring bridges between both C-16–C-10′ and C-2–O–C-9′. All compounds were deemed noncytotoxic (IC50 > 10 μM) when tested against A549 human lung adenocarcinoma cells.
- Published
- 2018
45. 2-(4-Chlorophenyl)-3,5-dimethyl-1λ6,2-thiazine-1,1-dione
- Author
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Hamid Khaledi, A. Hamid A. Hadi, Kamal Aziz Ketuly, and Rostam R. Braim
- Subjects
Crystallography ,QD901-999 - Abstract
In the title compound, C12H12ClNO2S, the S atom is displaced by 0.708 (2) Å out of the plane through the remaining atoms of the thiazine ring (r.m.s. deviation = 0.0823 Å). This plane makes a dihedral angle of 89.33 (7)° with the phenyl ring. In the crystal, adjacent molecules are connected through C—H...O hydrogen bonds into layers parallel to the bc plane.
- Published
- 2011
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46. 17-Deoxoestrone [estra-1,3,5(10)-trien-3-ol]–methanol (3/1)
- Author
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Kamal Aziz Ketuly, A. Hamid A. Hadi, Seik Weng Ng, and Edward R. T. Tiekink
- Subjects
Crystallography ,QD901-999 - Abstract
Three independent molecules of the title estrone derivative and a molecule of methanol comprise the asymmetric unit of the title compound [systematic name: 13-methyl-6,7,8,9,11,12,13,14,15,16-decahydrocyclopenta[a]phenanthren-3-ol–methanol (3/1)], 3C18H24O·CH3OH. Two of the estrone molecules exhibit 50:50 disorder (one displays whole-molecule disorder and the other partial disorder in the fused five- and six-membered rings) so that five (partial) molecular conformations are discernable. The conformation of the six-membered ring abutting the aromatic ring is close to a half-chair in all five components. The conformation of the six-membered ring fused to the five-membered ring is based on a chair with varying degrees of distortion ranging from minor to significant. Two distinct conformations are found for the five-membered ring: in four molecules, the five-membered ring is twisted about the bond linking it to the six-membered ring, and in the other, the five-membered ring is an envelope with the quaternary C atom being the flap atom. The crystal packing features O—H...O hydrogen bonding whereby the four molecules comprising the asymmetric unit are linked into a supramolecular chain along the b axis.
- Published
- 2011
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47. 3β-Acetoxy-6-hydroxyiminocholestane
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Kamal Aziz Ketuly, A. Hamid A. Hadi, Seik Weng Ng, and Edward R. T. Tiekink
- Subjects
Crystallography ,QD901-999 - Abstract
Two independent molecules comprise the asymmetric unit of the title cholestane derivative, C29H49NO3 {systematic name: (3S,8S,9S,10R,13R,14S,17R)-17-[(1R)-1,5-dimethylhexyl]-6-hydroxyimino-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-yl acetate}. The major differences between the molecules relate to the relative orientations of the terminal acetyl [C—C—O—C torsion angles = −158.8 (3) and −81.7 (3)°] and alkyl groups [C—C—C—C = 168.9 (3) and 65.8 (4)°]. In the crystal, the independent molecules associate via pairs of O—H...N hydrogen bonds, forming dimeric aggregates. Supramolecular layers in the ab plane are mediated by C—H...O interactions.
- Published
- 2011
- Full Text
- View/download PDF
48. Curcumenol from Curcuma zedoaria: a second monoclinic modification
- Author
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Omer Abdalla Ahmed Hamdi, Khalijah Awang, A. Hamid A. Hadi, Devi Rosmy Syamsir, and Seik Weng Ng
- Subjects
Crystallography ,QD901-999 - Abstract
The title compound, systematic name 9-isopropylidene-2,6-dimethyl-11-oxatricyclo[6.2.1.01,5]undec-6-en-8-ol, C15H22O2, which crystallizes with two molecules of similar conformation in the asymmetric unit, features three fused rings, two of which are five-membered and the third six-membered. Of the two five-membered rings, the one with an O atom has a distinct envelope shape (with the O atom representing the flap). The six-membered ring is also envelope-shaped as it shares a common O atom with the five-membered ring. In the crystal, the two independent molecules are linked by a pair of O—H...O hydrogen bonds, generating a dimer.
- Published
- 2010
- Full Text
- View/download PDF
49. Cabraleahydroxylactone from the leaves of Aglaia exima
- Author
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Seik Weng Ng, A. Hamid A. Hadi, Khalijah Awang, Khalit Mohamad, and Xe Min Loong
- Subjects
Crystallography ,QD901-999 - Abstract
Cabraleahydroxylactone, C27H44O3, isolated from the leaves of Aglaia exima, has three six-membered rings fused together that adopt chair conformations. Its two five-membered rings are enveloped shaped. The hydroxy group is in an axial position. It is a hydrogen-bond donor to the carbonyl O atom of an adjacent molecule; the O—H...O interactions lead to the formation of a helical chain that runs along the b axis. There are two independent molecules in the asymmetric unit.
- Published
- 2010
- Full Text
- View/download PDF
50. 3-Methyl-5α-cholest-2-ene
- Author
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Kamal Aziz Ketuly, A. Hamid A. Hadi, Seik Weng Ng, and Edward R. T. Tiekink
- Subjects
Crystallography ,QD901-999 - Abstract
In the title cholestane derivative, C28H48 [systematic name: (1S,2S,7R,10R,11R,14R,15R)-2,5,10,15-tetramethyl-14-[(2R)-6-methylheptan-2-yl]tetracyclo[8.7.0.02,7.011,15]heptadec-4-ene], the cyclohexene ring adopts a half-chair conformation. The parent 5α-cholest-2-ene and the equivalent fragment of the title compound are almost superimposable (r.m.s. deviation = 0.033 Å).
- Published
- 2010
- Full Text
- View/download PDF
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