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Concepts in Biochemistry: Chemical Synthesis of DNA.

Authors :
Caruthers, Marvin H.
Source :
Journal of Chemical Education. Jul 1989 66(7):577-580.
Publication Year :
1989

Abstract

Outlines the chemistry of the rapid synthesis of relatively large DNA fragments (100-200 monomers each) with yields exceeding 99 percent per coupling. DNA synthesis methodologies are outlined and a polymer-supported synthesis of DNA using deoxynucleoside phosphoramidites is described with structural formulas. (YP)

Details

Language :
English
Volume :
66
Issue :
7
Database :
ERIC
Journal :
Journal of Chemical Education
Publication Type :
Academic Journal
Accession number :
EJ398276
Document Type :
Journal Articles<br />Reports - Descriptive