Practical Teaching of Modeling Tools for Ion-Exchange Chromatography: A Case Study

The utilization of modeling tools has gained significant attention recently. These models typically involve a series of partial differential equations, which can be challenging for novice modelers. This paper presented an approach in practical teaching of modeling tools for ion-exchange chromatography with three parts: model introduction, model calibration through group learning using different calibration strategies, and model applications. This approach was integrated into the traditional bioseparation engineering curriculum as an activity, using the separation of monomer-dimer mixtures of monoclonal antibodies as an example. Results of competitive group learning and the Wilcoxon test revealed that the parameter-by-parameter method was more user-friendly than the Yamamoto method for novice modelers to obtain reasonable model parameters quickly. Then, students used the well-fitted model for process optimization and explored the effects of process parameters and material input variation on the chromatography process, which helped students appreciate the critical role of time and material savings achieved through modeling tools. Finally, the student questionnaire results revealed that over two-thirds of the students gave positive feedback on the activity. Through this practical teaching, students became familiar with chromatography modeling tools, moving away from the tedious formulaic descriptions found in traditional modeling courses. This well-designed activity can be expanded from academia to industry, transforming the novice modelers into experienced modelers who can meet the high demands of the modern biopharmaceutical industry.