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Amelioration of human acute lymphoblastic leukemia (ALL) cells by ZnO-TiO2-Chitosan-Amygdalin nanocomposites

Authors :
Elderdery, Abozer Y.
Alzahrani, Badr
Alanazi, Fehaid
Hamza, Siddiqa M.A.
Elkhalifa, Ahmed M.E.
Alhamidi, Abdulaziz H.
Alabdulsalam, Abdulrahim A.
Mohamedain, A.
Kumar, Suresh S.
Pooi, Ling Mok
Elderdery, Abozer Y.
Alzahrani, Badr
Alanazi, Fehaid
Hamza, Siddiqa M.A.
Elkhalifa, Ahmed M.E.
Alhamidi, Abdulaziz H.
Alabdulsalam, Abdulrahim A.
Mohamedain, A.
Kumar, Suresh S.
Pooi, Ling Mok
Publication Year :
2022

Abstract

The present study aims to study the cytotoxicity of ZnO-TiO2-Chitosan-Amygdalin nanocomposites (ZnO-TiO2-Chitosan-Amygdalin) on T lymphoblast cancer cells (MOLT-4). In a study, nanocomposites containing 2.5 to 15 µg/ml MTT were screened for their anticancer activity. Its anticancer properties were significantly higher than those of other nanocomposites with an IC50 value of 10.34 µg/ml. We studied the mechanism of action for cytotoxic cell death by fluorescence microscopy using Acridine Orange/EtBr (AO/EtBr) and Rhodamine 123 staining procedures. Using DCFH-DA, ZnO-TiO2-Chitosan-Amygdalin nanocomposites were analyzed to determine ROS production. The change in apoptotic protein expression for the 24 h following treatment with MOLT-4 cells for Caspase-3, 8, and 9. Nanocomposites containing ZnO-TiO2-Chitosan-Amygdalin increased the number of early and late apoptotic cells in MOLT-4 cells. ZnO-TiO2-Chitosan-Amygdalin nanocomposites also enhanced mitochondrial apoptosis through Caspase cascade signaling. MOLT-4 cells phosphorylated Caspase cascade in response to ZnO-TiO2-Chitosan-Amygdalin nanocomposites. Compared to the control group, the cancer cells treated with ZnO-TiO2-Chitosan-Amygdalin nanocomposites significantly arrest the proliferation and induces cleavage of pro-apoptotic proteins which leads to apoptotic cell death. Accordingly, ZnO-TiO2-Chitosan-Amygdalin nanocomposites might be effective against T lymphoblast cancer.

Details

Database :
OAIster
Publication Type :
Electronic Resource
Accession number :
edsoai.on1453488977
Document Type :
Electronic Resource