Photonics of Hydrothermally Treated ß-Lactoglobulin Amyloids

Increased temperature and high pressure are applied to beta-lactoglobulin fibrils in the autoclave, resulting in the acquisition of a composite material comprised of partially disassembled amyloid fibrils and carbon dots. Confirmation of the preservation of the beta-sheet motif attributed to amyloids in the hydrothermally treated fibrils is obtained through wide-angle X-ray scattering and ThT assay. Z-scan analysis reveals a two-photon absorption (2PA) enhancement in the low-lying transition band (La) of tyrosine, while quantum chemical calculations demonstrate a correlation between the yield of 2PA and the interspace distance between aromatic residues. Overall, the intrinsic optical properties of amyloid fibrils treated in a subcritical water environment are found to be linked with the pi-conjugation of tyrosine units and their through-space coupling. The resulting composite material is employed as a coating for a commercial ultraviolet light-emitting diode lamp, showcasing the potential utility of sustainable biomaterials with improved optical properties for photonics applications. By subjecting beta-lactoglobulin fibrils to elevated temperature and pressure in autoclave, partially disassembled fibrils are generated. The study reveals a correlation between fluorescence and two-photon absorption and the spacing of aromatic residues, shedding light on the mechanism behind the improved optical properties of amyloid fibrils. Furthermore, the hydrothermally treated beta-lactoglobulin fibrils are utilized to coat ultraviolet light-emitting diode lamps. image (c) 2024 WILEY-VCH GmbH
Funding Agencies|Polish National Agency for Academic Exchange Bekker programme; National Science Centre, Poland [2021/43/D/ST4/01741]; SNF [RPMA.01.01.00-14-8276/17]; European Union from the European Regional Development Fund under the Regional Operational Programme of the Mazowieckie Voivodeship [262695]; Research Council of Norway through its Centres of Excellence scheme [nn4654k]; NOTUR-The Norwegian Metacenter for Computational Science through a grant of computer time; [200769]