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Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Authors :
Dahl, Anja Lindis
Friis, Martin Barfred
Hallberg, Hanne Wiese
Kristiansen, Gitte Qvist
Holzknecht, Barbara Juliane
Dahl, Anja Lindis
Friis, Martin Barfred
Hallberg, Hanne Wiese
Kristiansen, Gitte Qvist
Holzknecht, Barbara Juliane
Source :
Dahl , A L , Friis , M B , Hallberg , H W , Kristiansen , G Q & Holzknecht , B J 2024 , ' Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth ' , Diagnostic Microbiology and Infectious Disease , vol. 109 , no. 2 , 116233 .
Publication Year :
2024

Abstract

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.<br />Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Details

Database :
OAIster
Journal :
Dahl , A L , Friis , M B , Hallberg , H W , Kristiansen , G Q & Holzknecht , B J 2024 , ' Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth ' , Diagnostic Microbiology and Infectious Disease , vol. 109 , no. 2 , 116233 .
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1439557551
Document Type :
Electronic Resource