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A bioaugmentation strategy to recover methane production under sulfate-stressed conditions:Highlights on targeted sulfate-reducing bacteria and DIET-related species
- Source :
- Giangeri , G , Tsapekos , P , Gaspari , M , Ghofrani-Isfahani , P , Treu , L , Kougias , P , Campanaro , S & Angelidaki , I 2024 , ' A bioaugmentation strategy to recover methane production under sulfate-stressed conditions : Highlights on targeted sulfate-reducing bacteria and DIET-related species ' , Applied Energy , vol. 362 , 122940 .
- Publication Year :
- 2024
-
Abstract
- Bioaugmentation has been recognized as a key strategy to improve the anaerobic digestion efficiency in organic waste treatment. Methanosarcina barkeri possesses direct interspecies electron transfer capability, a characteristic that allows it to outcompete other unwanted species such as sulfate-reducing bacteria. This study investigated the effects of bioaugmentation with Methanosarcina barkeri DSM800 on two continuous-stirred tank reactors fed with a sulfate-rich feedstock. One of the two reactors was supplemented with magnetite to facilitate direct interspecies electron transfer. Time series quantitative polymerase chain reactions were performed to evaluate the absolute abundance of crucial species, including the augmented Methanosarcina. Results showed increased and stabilized methane production of 22% and 21% in the reactor amended with magnetite and in the control reactor, respectively. Moreover, volatile fatty acids were almost completely consumed in the magnetite-supplemented reactor. The quantitative polymerase chain reaction was used to analyze the abundance of targeted species in response to bioaugmentation. Specifically, Methanosarcina barkeri was not retained in either reactor after one hydraulic retention time. Direct interspecies electron transfer-associated microorganisms showed opposite trends in the two reactors, highlighting the different interactions with Methanosarcina barkeri in the presence and absence of magnetite. Sulfate-reducing bacteria following the dissimilatory sulfate reduction pathway exhibited an opposite behavior in the reactor amended with magnetite, in contrast to those employing the assimilatory sulfate reduction pathway. Overall, the study demonstrated that bioaugmentation with exogenous archaea can considerably alter the microbial community, but the introduced species is not able to establish itself in a stable microbiome. In addition, the strategy could be further tested to control
Details
- Database :
- OAIster
- Journal :
- Giangeri , G , Tsapekos , P , Gaspari , M , Ghofrani-Isfahani , P , Treu , L , Kougias , P , Campanaro , S & Angelidaki , I 2024 , ' A bioaugmentation strategy to recover methane production under sulfate-stressed conditions : Highlights on targeted sulfate-reducing bacteria and DIET-related species ' , Applied Energy , vol. 362 , 122940 .
- Notes :
- application/pdf, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1439390041
- Document Type :
- Electronic Resource