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Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca2+ signals.

Authors :
Crapart, Cécile
Crapart, Cécile
Scott, Zubenelgenubi
Konno, Tasuku
Parutto, Pierre
Bailey, David
Westrate, Laura
Avezov, Edward
Koslover, Elena
Sharma, Aman
Crapart, Cécile
Crapart, Cécile
Scott, Zubenelgenubi
Konno, Tasuku
Parutto, Pierre
Bailey, David
Westrate, Laura
Avezov, Edward
Koslover, Elena
Sharma, Aman
Source :
Proceedings of the National Academy of Sciences; vol 121, iss 13
Publication Year :
2024

Abstract

The endoplasmic reticulum (ER) forms an interconnected network of tubules stretching throughout the cell. Understanding how ER functionality relies on its structural organization is crucial for elucidating cellular vulnerability to ER perturbations, which have been implicated in several neuronal pathologies. One of the key functions of the ER is enabling Ca[Formula: see text] signaling by storing large quantities of this ion and releasing it into the cytoplasm in a spatiotemporally controlled manner. Through a combination of physical modeling and live-cell imaging, we demonstrate that alterations in ER shape significantly impact its ability to support efficient local Ca[Formula: see text] releases, due to hindered transport of luminal content within the ER. Our model reveals that rapid Ca[Formula: see text] release necessitates mobile luminal buffer proteins with moderate binding strength, moving through a well-connected network of ER tubules. These findings provide insight into the functional advantages of normal ER architecture, emphasizing its importance as a kinetically efficient intracellular Ca[Formula: see text] delivery system.

Details

Database :
OAIster
Journal :
Proceedings of the National Academy of Sciences; vol 121, iss 13
Notes :
application/pdf, Proceedings of the National Academy of Sciences vol 121, iss 13
Publication Type :
Electronic Resource
Accession number :
edsoai.on1432081381
Document Type :
Electronic Resource