Description of four new Salinibacter species, two cultivated and named following the rules of the bacteriological code: Salinibacter pepae sp. nov., Salinibacter grassmerensis sp. nov.; and two uncultivated and named following the rules of the SeqCode: Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov. [Dataset]

Figure S1. Geographical distribution of the six locations studied here and their inter-location distances (given in the table). The distances given in each location refer to Mallorca. The hypersaline here are: Mallorca (Es Trenc and S’Avall and separated by 3.5 km) and Santa Pola in Spain, Fără Fund in Romania, Great Salt Lake (USA), Pampa (Laguna Colorada Chica and Laguna Colorada Grande, separated by 23 Km), and Lake Grassmere (New Zealand). Table S1. Statistics of Salinibacter MAGs recovered using different bioinformatic tools. MAGs selected for further analysis and taxonomic classification are marked in red. Text S1: In addition to the taxonomic study, here we assessed the different tools SPADES and MegaHIT assemblers, and MetaBAT binning tools selecting contigs with length > 2,000 pbs or > 5,000 pbs to retrieve the best quality MAG (Sup. Table S1). We observed that different tools and combinations rendered different qualities of the MAGs. The selection of contigs with length > 5,000 pbs binning rendered MAGs with a lower genome size, lower completeness and similar contamination. From the FF metagenome (Romania), the highest quality MAG was retrieved using the assembler SPADES and MetaBAT binning tool using a minimum contig length of 2,000 pbs. From CCH and CG metagenomes (Argentina), the highest quality MAGs were retrieved using the assembler MegaHIT and MetaBAT binning tool using a minimum contig length of 2,000 pbs (Sup. Table S1). Figure S2. Genomic clustering based on Average Amino-acid Identity (AAI) of genomes included in the study. Figure S3. Pangenome hierarchical clustering based on the presence (grey) or absence (black) of orthologous genes. Table S2. Pangenome statistics between genomes belonging to the same species. Table S3: CRISPR-Cas systems found in the Salinibacter pepae and Salinibacter grassmerensis genomes. Table S4. Cell morphologies of the new isolates. All cells have been cultivated onto MA agar. for 10 days at 12ºC. The morphology was ob