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Aquaporin 11 is related to cryotolerance and fertilising ability of frozen–thawed bull spermatozoa

Authors :
Universidade de Santiago de Compostela. Departamento de Patoloxía Animal
Morató, Roser
Prieto Martínez, Noelia
Muiño Otero, Rodrigo
Hidalgo, Carlos O.
Rodríguez-Gil, Joan E.
Bonet, Sergi
Yeste, Marc
Universidade de Santiago de Compostela. Departamento de Patoloxía Animal
Morató, Roser
Prieto Martínez, Noelia
Muiño Otero, Rodrigo
Hidalgo, Carlos O.
Rodríguez-Gil, Joan E.
Bonet, Sergi
Yeste, Marc
Publication Year :
2018

Abstract

Aquaporins (AQPs) are channel proteins involved in the transport of water and solutes across biological membranes. In the present study we identified and localised aquaporin 11 (AQP11) in bull spermatozoa and investigated the relationship between the relative AQP11 content, sperm cryotolerance and the fertilising ability of frozen–thawed semen. Bull ejaculates were classified into two groups of good and poor freezability and assessed through immunofluorescence and immunoblotting analyses before and after cryopreservation. AQP11 was localised throughout the entire tail and along the sperm head. These findings were confirmed through immunoblotting, which showed a specific band of approximately 50 kDa corresponding to AQP11. The relative amount of AQP11 was significantly (P < 0.05) higher in both fresh and frozen–thawed spermatozoa from bull ejaculates with good freezability compared with those with poorer freezability. In addition, in vitro oocyte penetration rates and non-return rates 56 days after AI were correlated with the relative AQP11 content in fresh spermatozoa. In conclusion, AQP11 is present in the head and tail of bull spermatozoa and its relative amount in fresh and frozen–thawed spermatozoa is related to the resilience of the spermatozoa to withstand cryopreservation and the fertilising ability of frozen–thawed spermatozoa. Further research is needed to elucidate the actual role of sperm AQP11 in bovine fertility.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1428034563
Document Type :
Electronic Resource