Operon-Expresser: The Innovated Gene Expression-Based Algorithm For Operon Structures In-Ference

Current biotechnological research of bacterial genomes has huge potential due to the useof next-generation sequencing (NGS) platforms. NGS era opens paths for analysis of data for sufficientdescription of microorganisms with ecology and biotechnology potential in the future. Althoughsome tools for inference of specific structures in bacterial genomes exist, their pipelines andmethodologies are often based only on searching bacterial genome databases and comparison withmodel microorganisms. This paper deals with the design of a new algorithm for operon structuresinference in bacterial genomes. The algorithm combines searching in bacterial databases and geneexpression information processing. The algorithm was implemented in R language and tested onClostridium beijerinckii NRRL B-598. The bacterium is a typical performer in the field of biofuelsproduction due to its ability to produce butanol. Thanks to that this bacterial organism can be ofgreat potential from an ecological and biotechnological point of view. The paper also provides acomparison of operon structures derived by Operon-mapper and its extending by Operon-expresser.