Spatial transcriptomics reveals distinct white matter pathology modulated by neutrophil/monocyte signaling following a single, focal cortical contusion injury in mice

Differential expressed gene (DEG) changes in the WM are consistent with a significant response to trauma in the GM and CC+EC WM but not AC Spatial transcriptomics reveals distinct white matter pathology modulated by neutrophil/monocyte signaling following a single, focal cortical contusion injury in mice Savannah K Kounelis-Wuillaume1, Camille Alba1,2, Andrew Frank3, Emilie Goguet4, William Brooks5Gauthaman Sukumar2, Matthew D. Wilkerson1,6, Clifton L. Dalgard1,2, Joseph McCabe1, Martin L. Doughty1. 1Department of Anatomy, Physiology, & Genetics, Uniformed Services University; 2The American Genome Center; 3Henry M. Jackson Foundation; 4Department Microbiology, Uniformed Services University; 5School of Medicine, Uniformed Services University; 6Center for Military Precision Health The Injured Cortex has a Substantial (Sub)Acute Neuroinflammatory Response Disclaimer. The opinions and assertions expressed herein are those of the author(s) and do not reflect the official policy or position of the Uniformed Services University of the Health Sciences or the Department of Defense. The authors have no conflicts of interest. This work was supported by the Brain Injury Association of America (BIAA) & a Cosmos Club Foundation Scholar Award (Kounelis-Wuillaume), a USU Award (Doughty) and the Department of Defense Program Objective Memorandum HU0001-20-20037 (Dalgard). Comparison 2 days post-injury 7days post-injury CCI Cortex vs Sham Cortex 6143 868 CCI CC+EC vs Sham CC+EC 1197 244 CCI AC vs Sham AC 43 34 CCI CC-EC vs CCI AC 632 1086 Sham CC-EC vs Sham AC 292 167  Damage to grey matter (GM) and white matter (WM) in traumatic brain injury (TBI) initiates spatiotemporally distinct pathophysiological processes  An early modulator of GM and WM injury are peripheral immune cells (PICs) that infiltrate damaged brain tissue during the acute post-injury period  We used Visium Spatial Transcriptomics (10x Genomics) to identify: 1. Gene expression changes in the damaged WM and GM of TB
RITM0038957
Damage to grey matter (GM) and white matter (WM) in traumatic brain injury (TBI) initiates spatiotemporally distinct pathophysiological processes ? An early modulator of GM and WM injury are peripheral immune cells (PICs) that infiltrate damaged brain tissue during the acute post-injury period ? We used Visium Spatial Transcriptomics (10x Genomics) to identify: 1. Gene expression changes in the damaged WM and GM of TBI mice 2. How these gene changes are affected by the infiltration of neutrophils and monocytes into the damaged brain