In vitro biological effects of clonal red wines

This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.