Oxidation of lactate to pyruvate mediates the cytotoxic potential of physical plasma-treated saline solutions in ovarian cancer

Epithelial ovarian cancer (EOC) is the most common type of gynecological tumor, presenting poor prognosis at diagnosis and with recurrences being frequently observed. Reactive species generated by physical plasma and transferred into liquids have shown promising results in cancer therapy. Recently, Ringer's lactate solution was exposed to plasma showing selective anticancer activity on EOC cells. In this work, we compared the effect of plasma treatment, using the kINPen plasma jet, on Ringer's saline and Ringer's lactate solution. These two plasma-treated liquids were analyzed chemically by quantifying reactive species and the extent of lactate oxidation. The biological efficiency of the plasma-treated liquids was explored in EOC cells. The results show that lactate is affected by plasma treatment, displaying a reduction of cytotoxic potential.
This publication is based upon work from COST Action Action CA20114 PlasTHER “Therapeutical Applications of Cold Plasmas,” supported by COST. The authors also acknowledge MINECO for PID2019‐103892RB‐I00/AEI/ 12 of 14 | BUCCI ET AL .16128869, 0, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/ppap.202300093 by Readcube (Labtiva Inc.), Wiley Online Library on [13/11/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 10.13039/501100011033 project and for PLEC2022‐ 009277/MCIN/AEI/10.13039/501100011033 funded by UE through NextGenerationEU/PRTR. The authors belong to SGR2017‐1165 (Cristina Canal, Miguel Mateu‐Sanz, and Francesco Tampieri) and acknowledge Generalitat de Catalunya for the ICREA Academia Award for Excellence in Research of Cristina Canal.
Peer Reviewed
Postprint (published version)