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Fully automated disc diffusion for rapid antibiotic susceptibility test results: a proof-of-principle study
- Source :
- Hombach, Michael; Jetter, Marion; Blöchliger, Nicolas; Kolesnik-Goldmann, Natalia; Böttger, Erik C (2017). Fully automated disc diffusion for rapid antibiotic susceptibility test results: a proof-of-principle study. Journal of Antimicrobial Chemotherapy, 72(6):1659-1668.
- Publication Year :
- 2017
-
Abstract
- Background Antibiotic resistance poses a significant threat to patients suffering from infectious diseases. Early readings of antibiotic susceptibility test (AST) results could be of critical importance to ensure adequate treatment. Disc diffusion is a well-standardized, established and cost-efficient AST procedure; however, its use in the clinical laboratory is hampered by the many manual steps involved, and an incubation time of 16-18 h, which is required to achieve reliable test results. Methods We have evaluated a fully automated system for its potential for early reading of disc diffusion diameters after 6-12 h of incubation. We assessed availability of results, methodological precision, categorical agreement and interpretation errors as compared with an 18 h standard. In total, 1028 clinical strains (291 Escherichia coli , 272 Klebsiella pneumoniae , 176 Staphylococcus aureus and 289 Staphylococcus epidermidis ) were included in this study. Disc diffusion plates were streaked, incubated and imaged using the WASPLab TM automation system. Results and conclusions Our results demonstrate that: (i) early AST reading is possible for important pathogens; (ii) methodological precision is not hampered at early timepoints; and (iii) species-specific reading times must be selected. As inhibition zone diameters change over time and are phenotype/drug combination dependent, specific cut-offs and expert rules will be essential to ensure reliable interpretation and reporting of early susceptibility testing results.
Details
- Database :
- OAIster
- Journal :
- Hombach, Michael; Jetter, Marion; Blöchliger, Nicolas; Kolesnik-Goldmann, Natalia; Böttger, Erik C (2017). Fully automated disc diffusion for rapid antibiotic susceptibility test results: a proof-of-principle study. Journal of Antimicrobial Chemotherapy, 72(6):1659-1668.
- Notes :
- application/pdf, info:doi/10.5167/uzh-137904, English, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1416164024
- Document Type :
- Electronic Resource