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Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins
- Source :
- Sensors 10(2010), 4739-4755
- Publication Year :
- 2010
-
Abstract
- The fabrication of novel uranyl (UO2 2+) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO2 2+ ions and showed minor interference from Ni2+, Cs+, Cd2+ and Co2+. Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO2 2+ binding, showing that both moieties are involved in the recognition to UO2 2+.
Details
- Database :
- OAIster
- Journal :
- Sensors 10(2010), 4739-4755
- Notes :
- application/pdf, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1415605933
- Document Type :
- Electronic Resource