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Subcellular architecture and metabolic connection in the planktonic photosymbiosis between Collodaria (radiolarians) and their microalgae

Authors :
Decelle, Johan
Veronesi, G.
LeKieffre, C.
Gallet, B.
Chevalier, F.
Stryhanyuk, Hryhoriy
Marro, S.
Ravanel, S.
Tucoulou, R.
Schieber, N.
Finazzi, G.
Schwab, Y.
Musat, Niculina
Decelle, Johan
Veronesi, G.
LeKieffre, C.
Gallet, B.
Chevalier, F.
Stryhanyuk, Hryhoriy
Marro, S.
Ravanel, S.
Tucoulou, R.
Schieber, N.
Finazzi, G.
Schwab, Y.
Musat, Niculina
Source :
ISSN: 1462-2912
Publication Year :
2021

Abstract

Photosymbiosis is widespread and ecologically important in the oceanic plankton but remains poorly studied. Here, we used multimodal subcellular imaging to investigate the photosymbiosis between colonial Collodaria and their microalga dinoflagellate (Brandtodinium). We showed that this symbiosis is very dynamic whereby symbionts interact with different host cells via extracellular vesicles within the colony. 3D electron microscopy revealed that the photosynthetic apparatus of the microalgae was more voluminous in symbiosis compared to free-living while the mitochondria volume was similar. Stable isotope probing coupled with NanoSIMS showed that carbon and nitrogen were stored in the symbiotic microalga in starch granules and purine crystals, respectively. Nitrogen was also allocated to the algal nucleolus. In the host, low 13C transfer was detected in the Golgi. Metal mapping revealed that intracellular iron concentration was similar in free-living and symbiotic microalgae (ca 40 ppm) and two-fold higher in the host, whereas copper concentration increased in symbionts and was detected in the host cell and extracellular vesicles. Sulfur concentration was around two times higher in symbionts (chromatin and pyrenoid) than their host. This study improves our understanding on the functioning of this oceanic photosymbiosis and paves the way for more studies to further assess its biogeochemical significance.

Details

Database :
OAIster
Journal :
ISSN: 1462-2912
Notes :
ISSN: 1462-2912, Environmental Microbiology 23 (11);; 6569 - 6586, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1406014225
Document Type :
Electronic Resource