Solid phase microextraction (SPME) used for direct fruit-body sampling in comparison with ASE-GC-MS to describing PAH's partitioning in mushroom cultures

Partition of fluorene, phenanthrene and pyrene in the cultures of the basidiomycete Pleurotus ostreatus (oyster mushroom) was determined by GC-MS analysis coupled with SPME directly within the growing mushroom fruit-body. Headspace (HS) SPME of the corresponding straw/mycelium culture provided information on the contents of PAHs remaining, thus enabling the transfer ratios of PAHs to be estimated. ASE-GC-MS was applied by way of comparison.