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Protocol for mapping double-stranded DNA break sites across the genome with translocation capture sequencing.
- Source :
- STAR Protocols; vol 4, iss 2
- Publication Year :
- 2023
-
Abstract
- Translocation sequencing can be used to assess mechanisms of DNA repair and identify genome-wide double-strand breaks (DSBs) accessible to DNA repair machinery. Here, we present a protocol for mapping double-strand DNA break sites across the genome with translocation capture sequencing. Bait DSBs are introduced using a Cas9 nuclease and repaired by the host cell, connecting bait DSBs to other DSBs. Repair sites are detected by isolating bait site DNA, cleaving normal sequence to enrich off-site repair, and next-generation sequencing. For complete details on the use and execution of this protocol, please refer to Switonski et al. (2021).1.
Details
- Database :
- OAIster
- Journal :
- STAR Protocols; vol 4, iss 2
- Notes :
- application/pdf, STAR Protocols vol 4, iss 2
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1401034896
- Document Type :
- Electronic Resource