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Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer

Authors :
Universidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicas
Herrero Filgueira, Carolina
Fuente González, Alexandre de la
Casas Arozamena, Carlos
Sebastián, Víctor
Prieto, Martín
Arruebo, Manuel
Abalo, Alicia
Colás, Eva
Moreno Bueno, Gema
Gil Moreno, Antonio
Vilar, Ana
Cueva, Juan
Abal Posada, Miguel
Muinelo Romay, Laura
Universidade de Santiago de Compostela. Departamento de Cirurxía e Especialidades Médico-Cirúrxicas
Herrero Filgueira, Carolina
Fuente González, Alexandre de la
Casas Arozamena, Carlos
Sebastián, Víctor
Prieto, Martín
Arruebo, Manuel
Abalo, Alicia
Colás, Eva
Moreno Bueno, Gema
Gil Moreno, Antonio
Vilar, Ana
Cueva, Juan
Abal Posada, Miguel
Muinelo Romay, Laura
Publication Year :
2019

Abstract

Tumor-derived extracellular vesicles (EVs) are secreted in large amounts into biological fluids of cancer patients. The analysis of EVs cargoes has been associated with patient´s outcome and response to therapy. However, current technologies for EVs isolation are tedious and low cost-efficient for routine clinical implementation. To explore the clinical value of circulating EVs analysis we attempted a proof-of-concept in endometrial cancer (EC) with ExoGAG, an easy to use and highly efficient new technology to enrich EVs. Technical performance was first evaluated using EVs secreted by Hec1A cells. Then, the clinical value of this strategy was questioned by analyzing the levels of two well-known tissue biomarkers in EC, L1 cell adhesion molecule (L1CAM) and Annexin A2 (ANXA2), in EVs purified from plasma in a cohort of 41 EC patients and 20 healthy controls. The results demonstrated the specific content of ANXA2 in the purified EVs fraction, with an accurate sensitivity and specificity for EC diagnosis. Importantly, high ANXA2 levels in circulating EVs were associated with high risk of recurrence and non-endometrioid histology suggesting a potential value as a prognostic biomarker in EC. These results also confirmed ExoGAG technology as a robust technique for the clinical implementation of circulating EVs analyses

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1400986992
Document Type :
Electronic Resource