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Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity.
- Source :
- Cell stem cell; vol 21, iss 1, 78-90.e6; 1934-5909
- Publication Year :
- 2017
-
Abstract
- Several cell populations have been reported to possess intestinal stem cell (ISC) activity during homeostasis and injury-induced regeneration. Here, we explored inter-relationships between putative mouse ISC populations by comparative RNA-sequencing (RNA-seq). The transcriptomes of multiple cycling ISC populations closely resembled Lgr5+ ISCs, the most well-defined ISC pool, but Bmi1-GFP+ cells were distinct and enriched for enteroendocrine (EE) markers, including Prox1. Prox1-GFP+ cells exhibited sustained clonogenic growth in vitro, and lineage-tracing of Prox1+ cells revealed long-lived clones during homeostasis and after radiation-induced injury in vivo. Single-cell mRNA-seq revealed two subsets of Prox1-GFP+ cells, one of which resembled mature EE cells while the other displayed low-level EE gene expression but co-expressed tuft cell markers, Lgr5 and Ascl2, reminiscent of label-retaining secretory progenitors. Our data suggest that the EE lineage, including mature EE cells, comprises a reservoir of homeostatic and injury-inducible ISCs, extending our understanding of cellular plasticity and stemness.
Details
- Database :
- OAIster
- Journal :
- Cell stem cell; vol 21, iss 1, 78-90.e6; 1934-5909
- Notes :
- application/pdf, Cell stem cell vol 21, iss 1, 78-90.e6 1934-5909
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1391613337
- Document Type :
- Electronic Resource