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Hepatitis C Virus in people with experience of injection drug use following their displacement to Southern Ukraine before 2020.

Authors :
Yakovleva, Anna
Yakovleva, Anna
Kovalenko, Ganna
Redlinger, Matthew
Smyrnov, Pavlo
Tymets, Olga
Korobchuk, Anna
Kotlyk, Lyudmyla
Kolodiazieva, Anna
Podolina, Anna
Cherniavska, Svitlana
Antonenko, Petro
Strathdee, Steffanie A
Friedman, Samuel R
Goodfellow, Ian
Wertheim, Joel O
Bortz, Eric
Meredith, Luke
Vasylyeva, Tetyana I
Yakovleva, Anna
Yakovleva, Anna
Kovalenko, Ganna
Redlinger, Matthew
Smyrnov, Pavlo
Tymets, Olga
Korobchuk, Anna
Kotlyk, Lyudmyla
Kolodiazieva, Anna
Podolina, Anna
Cherniavska, Svitlana
Antonenko, Petro
Strathdee, Steffanie A
Friedman, Samuel R
Goodfellow, Ian
Wertheim, Joel O
Bortz, Eric
Meredith, Luke
Vasylyeva, Tetyana I
Source :
BMC infectious diseases; vol 23, iss 1, 446; 1471-2334
Publication Year :
2023

Abstract

BackgroundDue to practical challenges associated with genetic sequencing in low-resource environments, the burden of hepatitis C virus (HCV) in forcibly displaced people is understudied. We examined the use of field applicable HCV sequencing methods and phylogenetic analysis to determine HCV transmission dynamics in internally displaced people who inject drugs (IDPWID) in Ukraine.MethodsIn this cross-sectional study, we used modified respondent-driven sampling to recruit IDPWID who were displaced to Odesa, Ukraine, before 2020. We generated partial and near full length genome (NFLG) HCV sequences using Oxford Nanopore Technology (ONT) MinION in a simulated field environment. Maximum likelihood and Bayesian methods were used to establish phylodynamic relationships.ResultsBetween June and September 2020, we collected epidemiological data and whole blood samples from 164 IDPWID (PNAS Nexus.2023;2(3):pgad008). Rapid testing (Wondfo® One Step HCV; Wondfo® One Step HIV1/2) identified an anti-HCV seroprevalence of 67.7%, and 31.1% of participants tested positive for both anti-HCV and HIV. We generated 57 partial or NFLG HCV sequences and identified eight transmission clusters, of which at least two originated within a year and a half post-displacement.ConclusionsLocally generated genomic data and phylogenetic analysis in rapidly changing low-resource environments, such as those faced by forcibly displaced people, can help inform effective public health strategies. For example, evidence of HCV transmission clusters originating soon after displacement highlights the importance of implementing urgent preventive interventions in ongoing situations of forced displacement.

Details

Database :
OAIster
Journal :
BMC infectious diseases; vol 23, iss 1, 446; 1471-2334
Notes :
application/pdf, BMC infectious diseases vol 23, iss 1, 446 1471-2334
Publication Type :
Electronic Resource
Accession number :
edsoai.on1391573685
Document Type :
Electronic Resource