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Systematically evaluating DOTATATE and FDG as PET immuno-imaging tracers of cardiovascular inflammation

Authors :
Toner, Yohana C.
Ghotbi, Adam A.
Naidu, Sonum
Sakurai, Ken
van Leent, Mandy M.T.
Jordan, Stefan
Ordikhani, Farideh
Amadori, Letizia
Sofias, Alexandros Marios
Fisher, Elizabeth L.
Maier, Alexander
Sullivan, Nathaniel
Munitz, Jazz
Senders, Max L.
Mason, Christian
Reiner, Thomas
Soultanidis, Georgios
Tarkin, Jason M.
Rudd, James H.F.
Giannarelli, Chiara
Ochando, Jordi
Pérez-Medina, Carlos
Kjaer, Andreas
Mulder, Willem J.M.
Fayad, Zahi A.
Calcagno, Claudia
Toner, Yohana C.
Ghotbi, Adam A.
Naidu, Sonum
Sakurai, Ken
van Leent, Mandy M.T.
Jordan, Stefan
Ordikhani, Farideh
Amadori, Letizia
Sofias, Alexandros Marios
Fisher, Elizabeth L.
Maier, Alexander
Sullivan, Nathaniel
Munitz, Jazz
Senders, Max L.
Mason, Christian
Reiner, Thomas
Soultanidis, Georgios
Tarkin, Jason M.
Rudd, James H.F.
Giannarelli, Chiara
Ochando, Jordi
Pérez-Medina, Carlos
Kjaer, Andreas
Mulder, Willem J.M.
Fayad, Zahi A.
Calcagno, Claudia
Source :
Toner , Y C , Ghotbi , A A , Naidu , S , Sakurai , K , van Leent , M M T , Jordan , S , Ordikhani , F , Amadori , L , Sofias , A M , Fisher , E L , Maier , A , Sullivan , N , Munitz , J , Senders , M L , Mason , C , Reiner , T , Soultanidis , G , Tarkin , J M , Rudd , J H F , Giannarelli , C , Ochando , J , Pérez-Medina , C , Kjaer , A , Mulder , W J M , Fayad , Z A & Calcagno , C 2022 , ' Systematically evaluating DOTATATE and FDG as PET immuno-imaging tracers of cardiovascular inflammation ' , Scientific Reports , vol. 12 , no. 1 , 6185 .
Publication Year :
2022

Abstract

In recent years, cardiovascular immuno-imaging by positron emission tomography (PET) has undergone tremendous progress in preclinical settings. Clinically, two approved PET tracers hold great potential for inflammation imaging in cardiovascular patients, namely FDG and DOTATATE. While the former is a widely applied metabolic tracer, DOTATATE is a relatively new PET tracer targeting the somatostatin receptor 2 (SST2). In the current study, we performed a detailed, head-to-head comparison of DOTATATE-based radiotracers and [18F]F-FDG in mouse and rabbit models of cardiovascular inflammation. For mouse experiments, we labeled DOTATATE with the long-lived isotope [64Cu]Cu to enable studying the tracer’s mode of action by complementing in vivo PET/CT experiments with thorough ex vivo immunological analyses. For translational PET/MRI rabbit studies, we employed the more widely clinically used [68Ga]Ga-labeled DOTATATE, which was approved by the FDA in 2016. DOTATATE’s pharmacokinetics and timed biodistribution were determined in control and atherosclerotic mice and rabbits by ex vivo gamma counting of blood and organs. Additionally, we performed in vivo PET/CT experiments in mice with atherosclerosis, mice subjected to myocardial infarction and control animals, using both [64Cu]Cu-DOTATATE and [18F]F-FDG. To evaluate differences in the tracers’ cellular specificity, we performed ensuing ex vivo flow cytometry and gamma counting. In mice subjected to myocardial infarction, in vivo [64Cu]Cu-DOTATATE PET showed higher differential uptake between infarcted (SUVmax 1.3, IQR, 1.2–1.4, N = 4) and remote myocardium (SUVmax 0.7, IQR, 0.5–0.8, N = 4, p = 0.0286), and with respect to controls (SUVmax 0.6, IQR, 0.5–0.7, N = 4, p = 0.0286), than [18F]F-FDG PET. In atherosclerotic mice, [64Cu]Cu-DOTATATE PET aortic signal, but not [18F]F-FDG PET, was hi

Details

Database :
OAIster
Journal :
Toner , Y C , Ghotbi , A A , Naidu , S , Sakurai , K , van Leent , M M T , Jordan , S , Ordikhani , F , Amadori , L , Sofias , A M , Fisher , E L , Maier , A , Sullivan , N , Munitz , J , Senders , M L , Mason , C , Reiner , T , Soultanidis , G , Tarkin , J M , Rudd , J H F , Giannarelli , C , Ochando , J , Pérez-Medina , C , Kjaer , A , Mulder , W J M , Fayad , Z A & Calcagno , C 2022 , ' Systematically evaluating DOTATATE and FDG as PET immuno-imaging tracers of cardiovascular inflammation ' , Scientific Reports , vol. 12 , no. 1 , 6185 .
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1382517965
Document Type :
Electronic Resource